12 results about "MOLECULAR BIOLOGY METHODS" patented technology

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

The invention relates to a specific real-time fluorescent PCR (polymerase chain reaction) amplification primer, a probe and a detection method for identifying lutjanus purpureus, and belongs to the technical field of molecular detection and species identification. According to the invention, the specific primer and the probe for lutjanus purpuratus are designed, and the real-time fluorescent PCR technology is combined, so that the lutjanus purpuratus can be quickly and accurately identified. The method has the advantages of high specificity, high sensitivity, simplicity and convenience in operation and the like, and a reliable molecular biological method is provided for species identification of lutjanus purpurea.

The application discloses a pangolin species identification method based on nail piece element characteristics, which comprises the following steps: step S1: collecting nail piece samples covering existing 10 kinds of pangolin species, and obtaining initial data of multi-element characteristics of the nail piece samples; step S2: identifying the species of the nail piece samples by a molecular biology method, and obtaining the species labels of each nail piece sample; step S3: constructing an "element characteristic-species label" standard data set; step S4: selecting a table prior fitting network as a core machine learning algorithm, training a model by using training set data, and constructing the model; and step S5: identifying whether a to-be-detected sample belongs to a pangolin nail piece according to morphological judgment. The method has a single sample data collection time of less than 60 seconds, does not need to damage the sample, is used for nail piece identification of samples without prior clues, and the overall accuracy of a global ten-species classification model reaches 0.93.

A novel circRNA, its uses, and a reagent kit are disclosed, relating to the field of animal genetics and breeding. The nucleotide sequence of Circ-06958 of this invention is shown in SEQ ID NO.1 of the sequence listing. Circ-06958 is used in the preparation of a reagent for the proliferation of porcine skeletal muscle satellite cells. This invention successfully cloned and identified a novel circRNA sequence—Circ-06958. Molecular biological methods confirmed that Circ-06958 is a marker for the proliferation of porcine skeletal muscle satellite cells, and increasing the expression level of Circ-06958 can promote the proliferation of porcine skeletal muscle satellite cells.

The application of Circ-21490 in proliferation of pig skeletal muscle satellite cells relates to the field of molecular genetics, the application of circRNA in proliferation of pig skeletal muscle satellite cells, the circRNA is Circ-21490, the nucleotide sequence of Circ-21490 is shown as SEQ ID No:1.The application successfully clones and identifies a new circRNA sequence-Circ-21490, determines that Circ-21490 is a marker for proliferation of pig skeletal muscle satellite cells through a molecular biology method, and overexpression of Circ-21490 can promote proliferation of pig skeletal muscle satellite cells.A detection kit containing a specific primer pair of Circ-21490 is designed, the expression level of Circ-21490 is analyzed by using the kit, so as to identify the proliferation potential of pig skeletal muscle satellite cells.

The invention discloses a Wikstrolide E mediated psoriasis animal model and a construction method thereof, and belongs to the technical field of animal models. Aiming at the problem that the application of the existing psoriasis model is limited, a novel psoriasis animal model is constructed by utilizing a compound Wikstrolide E, and psoriasis characteristics which can be simulated by the animal model are evaluated through PASI scoring, histopathology, dermoscopy and a molecular biological method. The model is expected to become an extremely valuable tool in the fields of psoriasis research and drug discovery.

This invention relates to the fields of microbial technology and animal pathogen control technology, specifically to an endophytic fungal extract and its application against *Glassonella parasuis*. The specific technical solution is as follows: the endophytic fungal strain is derived from *Fritillaria cirrhosa* from Hubei Province, identified as a *Talaromyces* fungus by molecular biological methods, and deposited at the China Center for Type Culture Collection (CCTCC). Studies have shown that the fermentation product of this strain and the extract obtained by ethyl acetate extraction are effective against *Glassonella parasuis* (…). Glaesserella parasuis It exhibits significant inhibitory effects, effectively suppressing its growth and reducing its pathogenic risk. The endophytic fungal strains and their extracts described in this invention can be used to prepare antibacterial agents against *Glassonella parasuis*, showing promising application prospects in the field of livestock disease control.

The application discloses application of MST1 interfering RNA in promoting in-vitro differentiation of ESCs into insulin-secreting cells, and belongs to the technical field of biotechnology. In the process of in-vitro directional differentiation of rat ESCs into beta cells, the application infects by using an MST1 shRNA lentivirus vector, and indexes are detected and functions are determined by using cell biology and molecular biology methods, and it is found for the first time that the efficiency of inducing ESCs to differentiate into beta cell-like cells can be improved by reducing the expression of MST1. Meanwhile, how MST1 realizes the regulation of beta cell maturation by means of PDX1 in the process of directional differentiation of rat ESCs into beta cells is described, the reason affecting in-vitro maturation of beta cells is discussed, and thus the system of directional differentiation of ESCs into mature beta cells is optimized, which has important significance for obtaining beta cells with normal insulin secretion function in-vitro.

Vibrio parahaemolyticus serotype O10 is an important foodborne pathogen with a very high isolation rate in my country in recent years. This invention provides a specific oligonucleotide sequence for detecting Vibrio parahaemolyticus serotype O10 and its application. The oligonucleotides are DNA fragments selected from the IS1 and orf9 genes of the O antigen gene cluster of Vibrio parahaemolyticus serotype O10. This invention also establishes a PCR detection method for Vibrio parahaemolyticus serotype O10 using the above-mentioned oligonucleotides, establishing for the first time a technique for detecting this bacterium using molecular biology methods. This is of great significance for the identification and epidemiological monitoring of this important foodborne pathogen.

This invention discloses a molecular biology method and its application for simultaneously detecting five poultry-derived components. Using primers shown in SEQ ID NO.1 and SEQ ID NO.2, genomic DNA of the sample to be tested is amplified by PCR. After capillary electrophoresis, sequence alignment is performed. A peak value of 234 bp indicates a chicken-derived component; a peak value of 271 bp indicates a quail-derived component; a peak value of 229 bp indicates a turkey-derived component; a peak value of 252 bp indicates a partridge-derived component; and a peak value of 223 bp indicates a pheasant-derived component. Peak values ​​of 234 bp, 271 bp, 229 bp, 252 bp, and 223 bp indicate the simultaneous presence of five poultry-derived components. The detection kit developed based on this method can generate considerable economic benefits and good social value.

The invention belongs to the technical field of insect resistance research, and discloses a method for enhancing insect resistance of poplar by using a leaf aging related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene: (2) designing a guide gRNA sequence; (3) constructing a targeted PtNAC109 gene editing carrier plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) infecting the populus tomentosa plants with agrobacterium; (6) screening calluses with resistance; (7) inducing, prolonging and rooting resistant buds; (8) verifying a gene editing effect by utilizing a molecular biology means; and (9) obtaining a poplar gene editing new germplasm with enhanced insect resistance.

The application provides a compound for treating epilepsy and a preparation method thereof. The compound of the application exhibits significant therapeutic effect in the field of epilepsy treatment, has a strong and effective inhibitory effect on the onset of epilepsy, and has extremely excellent therapeutic effect and leads the CBD group in all indexes according to the data analysis of the detection of the expression of apoptosis-related proteins from the evaluation of the molecular biology method. In addition, the compound can effectively prolong the latency of the onset of epilepsy, thereby reducing the frequency of taking medicine and greatly reducing the toxic side effects possibly caused by the medicine. The new compound provided by the application improves the safety and effectiveness of the treatment of epilepsy in many aspects, and opens up a new direction with great potential in the field of epilepsy treatment.