25 results about "MOLECULAR BIOLOGY METHODS" patented technology

The invention discloses application of 4-isopropyltoluene to improvement of depression-like behaviors of mice. An experiment adopts a chronic constraint stress method to construct a depression model, through behavioral detection such as a sucrose preference test, a tail suspension test and a novel inhibition ingestion test, and in combination with molecular biology means such as immunofluorescent staining and Western blot, the anti-depression effect and potential mechanism of 4-isopropyltoluene are systematically evaluated, and the anti-depression effect and potential mechanism of 4-isopropyltoluene are evaluated. Results show that 4-isopropyltoluene plays a significant anti-depression role by inhibiting neuroinflammation, enhancing glial cell support function and activating AKT signal pathway, and has the potential of being developed into a novel anti-depression candidate drug.

A molecular biology method, by which relative (normalized to an internal control) N6-methyladenine (6mA) level at a selected specific genomic site in a tissue sample containing numerous individual cellular genomes is accurately determined, and this level is projected to a reference “6mA level-age” curve determined earlier. The relative 6mA level of the examined individual assigns her / his (biological) age on the curve. The reference curve was previously established by determining 6mA levels of a large (>1000) number of healthy individuals with known age. On the reference curve, the relative 6mA level correlates with age; the higher the 6mA level at the tested genomic site, the higher the age of the individual analyzed. The accuracy of the measurement depends on the accuracy of the method by which 6mA level is determined.

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

The invention relates to a specific real-time fluorescent PCR (polymerase chain reaction) amplification primer, a probe and a detection method for identifying lutjanus purpureus, and belongs to the technical field of molecular detection and species identification. According to the invention, the specific primer and the probe for lutjanus purpuratus are designed, and the real-time fluorescent PCR technology is combined, so that the lutjanus purpuratus can be quickly and accurately identified. The method has the advantages of high specificity, high sensitivity, simplicity and convenience in operation and the like, and a reliable molecular biological method is provided for species identification of lutjanus purpurea.

The invention discloses a wheat heat resistance related protein TaHSC702 as well as a coding gene and application thereof, the function of the wheat heat resistance related protein TaHSC702 is deeply researched through genetics and molecular biology methods, the effect of the TaHSC702 gene in regulating and controlling the heat resistance of wheat is clarified, two different types of TaHSC702 mutants are created through EMS mutagenesis, and the wheat heat resistance related protein TaHSC702 is obtained. The two mutant proteins can significantly improve the heat resistance of wheat. The important significance is realized on the breeding of the heat-resistant wheat.

The application discloses a pangolin species identification method based on nail piece element characteristics, which comprises the following steps: step S1: collecting nail piece samples covering existing 10 kinds of pangolin species, and obtaining initial data of multi-element characteristics of the nail piece samples; step S2: identifying the species of the nail piece samples by a molecular biology method, and obtaining the species labels of each nail piece sample; step S3: constructing an "element characteristic-species label" standard data set; step S4: selecting a table prior fitting network as a core machine learning algorithm, training a model by using training set data, and constructing the model; and step S5: identifying whether a to-be-detected sample belongs to a pangolin nail piece according to morphological judgment. The method has a single sample data collection time of less than 60 seconds, does not need to damage the sample, is used for nail piece identification of samples without prior clues, and the overall accuracy of a global ten-species classification model reaches 0.93.

A novel circRNA, its uses, and a reagent kit are disclosed, relating to the field of animal genetics and breeding. The nucleotide sequence of Circ-06958 of this invention is shown in SEQ ID NO.1 of the sequence listing. Circ-06958 is used in the preparation of a reagent for the proliferation of porcine skeletal muscle satellite cells. This invention successfully cloned and identified a novel circRNA sequence—Circ-06958. Molecular biological methods confirmed that Circ-06958 is a marker for the proliferation of porcine skeletal muscle satellite cells, and increasing the expression level of Circ-06958 can promote the proliferation of porcine skeletal muscle satellite cells.

The invention discloses a wheat heat resistance related protein TaHSC701 as well as an encoding gene and application thereof, the function of the wheat heat resistance related protein TaHSC701 is deeply researched through genetics and molecular biology methods, the effect of the TaHSC701 gene in regulating and controlling the heat resistance of wheat is defined, and the wheat heat resistance related protein TaHSC701 has important significance in breeding of heat-resistant wheat.

The invention belongs to the technical field of microbiological detection, and particularly relates to a method for rapidly detecting viable salmonella and application. The method comprises the following steps: mixing a sample to be detected with Felix O-1 bacteriophage, incubating at 37 DEG C to enable the Felix O-1 bacteriophage to infect and proliferate in the sample to be detected, and performing splitting decomposition treatment after infection proliferation is finished to obtain proliferated Felix O-1 bacteriophage DNA (Deoxyribose Nucleic Acid); felix O-1 bacteriophage DNA is used as a template for real-time fluorescence RPA amplification, and whether viable salmonella exists or not is judged through fluorescence signal intensity. According to the method provided by the invention, the defects that the traditional bacterial culture and biochemical confirmation method is long in time consumption and the original molecular biological method cannot conveniently distinguish dead and live are overcome. The method is short in detection time, the RPA reaction is carried out under a constant-temperature condition, equipment requirements are simple, and the method is suitable for field detection.

The application of Circ-21490 in proliferation of pig skeletal muscle satellite cells relates to the field of molecular genetics, the application of circRNA in proliferation of pig skeletal muscle satellite cells, the circRNA is Circ-21490, the nucleotide sequence of Circ-21490 is shown as SEQ ID No:1.The application successfully clones and identifies a new circRNA sequence-Circ-21490, determines that Circ-21490 is a marker for proliferation of pig skeletal muscle satellite cells through a molecular biology method, and overexpression of Circ-21490 can promote proliferation of pig skeletal muscle satellite cells.A detection kit containing a specific primer pair of Circ-21490 is designed, the expression level of Circ-21490 is analyzed by using the kit, so as to identify the proliferation potential of pig skeletal muscle satellite cells.

The invention discloses a mitochondrial cox gene segment for detecting Cailanthus splendens, application of the mitochondrial cox gene segment and a method for detecting leaf spot of oak trees. According to the present invention, a strain of Ciocereus ciliatus LDCQJJ4 from a oak leaf spot disease sample is obtained, the preservation number of the strain is GDMCC No.3.1250, the specific fragment of the mitochondrial cox gene of the strain is obtained through research, and the nucleotide sequence of the specific fragment is represented by SEQ ID NO.5, such that the specific fragment can be used for the specific detection of the pathogenic bacteria so as to achieve the specific detection and diagnosis of the oak leaf spot caused by the Ciocereus ciliatus LDCQJJ4, and the specific detection and the diagnosis of the oak leaf spot caused by the Ciocereus ciliatus LDCQJJ4 can be provided for the oak leaf spot caused by the Ciocereus ciliatus LDCQJJ4. The invention further provides a specific primer and a kit aiming at the gene segment, and the primer is high in specificity and sensitivity. According to the method, a molecular biology means is used for detecting the ailanthus slaflock bacillus megatherium for the first time, the detection method is specific, sensitive, rapid, simple and convenient, results are reliable, diseases can be found as early as possible, corresponding prevention and control measures can be taken in time, and the influence of leaf spot on the yield and quality of oak trees is reduced.

The invention discloses a Wikstrolide E mediated psoriasis animal model and a construction method thereof, and belongs to the technical field of animal models. Aiming at the problem that the application of the existing psoriasis model is limited, a novel psoriasis animal model is constructed by utilizing a compound Wikstrolide E, and psoriasis characteristics which can be simulated by the animal model are evaluated through PASI scoring, histopathology, dermoscopy and a molecular biological method. The model is expected to become an extremely valuable tool in the fields of psoriasis research and drug discovery.

This invention relates to the fields of microbial technology and animal pathogen control technology, specifically to an endophytic fungal extract and its application against *Glassonella parasuis*. The specific technical solution is as follows: the endophytic fungal strain is derived from *Fritillaria cirrhosa* from Hubei Province, identified as a *Talaromyces* fungus by molecular biological methods, and deposited at the China Center for Type Culture Collection (CCTCC). Studies have shown that the fermentation product of this strain and the extract obtained by ethyl acetate extraction are effective against *Glassonella parasuis* (…). Glaesserella parasuis It exhibits significant inhibitory effects, effectively suppressing its growth and reducing its pathogenic risk. The endophytic fungal strains and their extracts described in this invention can be used to prepare antibacterial agents against *Glassonella parasuis*, showing promising application prospects in the field of livestock disease control.

The application discloses application of MST1 interfering RNA in promoting in-vitro differentiation of ESCs into insulin-secreting cells, and belongs to the technical field of biotechnology. In the process of in-vitro directional differentiation of rat ESCs into beta cells, the application infects by using an MST1 shRNA lentivirus vector, and indexes are detected and functions are determined by using cell biology and molecular biology methods, and it is found for the first time that the efficiency of inducing ESCs to differentiate into beta cell-like cells can be improved by reducing the expression of MST1. Meanwhile, how MST1 realizes the regulation of beta cell maturation by means of PDX1 in the process of directional differentiation of rat ESCs into beta cells is described, the reason affecting in-vitro maturation of beta cells is discussed, and thus the system of directional differentiation of ESCs into mature beta cells is optimized, which has important significance for obtaining beta cells with normal insulin secretion function in-vitro.

The invention discloses a filling gene for increasing corn kernel weight and application thereof, expression or activity of ZmMYB29 or homologues thereof is up-regulated in corn, and corn characters can be improved: (i) filling of corn kernels is promoted; (ii) increasing the weight of the corn kernels; (iii) increasing the yield or biomass of corn; (iv) the starch content in the corn kernels is increased. Researches based on methods of genetics, molecular biology and the like find that the ZmMYB29 gene has a positive regulation effect on filling of corn kernels, and when expression of the gene is up-regulated in corn, filling of the corn kernels can be remarkably promoted, the weight of the corn kernels is increased, and the corn kernels become larger; meanwhile, the ZmMYB29 also finds that the starch content can be obviously increased in the process of regulating and controlling the grain starch by the ZmMYB29. The invention provides a new choice for collaborative improvement of quality and yield of cereal crops.

Vibrio parahaemolyticus serotype O10 is an important foodborne pathogen with a very high isolation rate in my country in recent years. This invention provides a specific oligonucleotide sequence for detecting Vibrio parahaemolyticus serotype O10 and its application. The oligonucleotides are DNA fragments selected from the IS1 and orf9 genes of the O antigen gene cluster of Vibrio parahaemolyticus serotype O10. This invention also establishes a PCR detection method for Vibrio parahaemolyticus serotype O10 using the above-mentioned oligonucleotides, establishing for the first time a technique for detecting this bacterium using molecular biology methods. This is of great significance for the identification and epidemiological monitoring of this important foodborne pathogen.

This invention discloses a molecular biology method and its application for simultaneously detecting five poultry-derived components. Using primers shown in SEQ ID NO.1 and SEQ ID NO.2, genomic DNA of the sample to be tested is amplified by PCR. After capillary electrophoresis, sequence alignment is performed. A peak value of 234 bp indicates a chicken-derived component; a peak value of 271 bp indicates a quail-derived component; a peak value of 229 bp indicates a turkey-derived component; a peak value of 252 bp indicates a partridge-derived component; and a peak value of 223 bp indicates a pheasant-derived component. Peak values ​​of 234 bp, 271 bp, 229 bp, 252 bp, and 223 bp indicate the simultaneous presence of five poultry-derived components. The detection kit developed based on this method can generate considerable economic benefits and good social value.

The invention belongs to the technical field of insect resistance research, and discloses a method for enhancing insect resistance of poplar by using a leaf aging related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene: (2) designing a guide gRNA sequence; (3) constructing a targeted PtNAC109 gene editing carrier plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) infecting the populus tomentosa plants with agrobacterium; (6) screening calluses with resistance; (7) inducing, prolonging and rooting resistant buds; (8) verifying a gene editing effect by utilizing a molecular biology means; and (9) obtaining a poplar gene editing new germplasm with enhanced insect resistance.

1. The name of the design product: full-automatic molecular detection analyzer. 2. The use of the design product: for clinical and scientific research fields, applied to molecular biology method for detecting molecular biomarkers in samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses a molecular biological method capable of simultaneously detecting five poultry-derived components and application of the molecular biological method. PCR amplification and capillary electrophoresis are carried out on genome DNA of a sample to be detected through primers shown in SEQ ID NO.1 and SEQ ID NO.2, sequence comparison is carried out, when the peak value is 234 bp, the sample contains chicken-derived components, when the peak value is 271 bp, the sample contains quail-derived components, and when the peak value is 229 bp, the sample contains turkey-derived components. When the peak value is 252bp, the partridge-derived component is contained, when the peak value is 223bp, the pheasant-derived component is contained, and when the peak values are 234bp, 271bp, 229bp, 252bp and 223bp, the five poultry-derived components are contained at the same time. The detection kit developed on the basis of the method can generate considerable economic benefits and good social values.

The invention provides a bacterial laccase mutant. The amino acid sequence of the bacterial laccase mutant is as shown in SEQ ID NO. 2. The invention belongs to the technical field of enzyme engineering, low-conservative amino acids are screened through multi-sequence alignment and site specific conservative index calculation, and four potential functional plasticity sites are screened by further combining an enzyme three-dimensional structure and a molecular docking experiment; furthermore, mutants are constructed by adopting a molecular biological method, the catalytic efficiency of each bacterial laccase mutant is quantitatively evaluated, the bacterial laccase mutant with relatively good stability and relatively high degradation efficiency is finally screened, and the degradation efficiency of the bacterial laccase on sulfadiazine can be remarkably improved under the condition of not depending on a mediator.

The application discloses a human-mouse chimeric monoclonal antibody of NF186 protein, a product thereof and a method for detecting the NF186 protein, and belongs to the technical field of monoclonal antibodies. The application first obtains a mouse monoclonal antibody sequence of the NF186 protein through a molecular biology method, then the mouse monoclonal antibody sequence is reformed to prepare the human-mouse chimeric monoclonal antibody of the NF186 protein, the human-mouse chimeric antibody can be expressed and purified on a large scale by using a recombinant protein expression and purification technology, a large amount of the antibody can be obtained at one time at a low cost, and the batch difference and the batch-to-batch difference can be controlled and kept consistent. The application can effectively solve the technical problems that the current mouse / rabbit single / multiclonal antibodies have batch-to-batch differences, cannot form low-cost large-scale industrial production, and the Fc region cannot react with an anti-human IgG antibody.

A molecular biology method, by which relative (normalized to an internal control) N6-methyladenine (6 mA) level at a selected site of the mitochondrial (mt) DNA (deoxyribonucleic acid) (mtDNA) in a tissue sample is accurately determined, and this level is projected to a reference “relative mtDNA 6 mA level-age” curve constructed earlier is described. On the reference curve, relative mtDNA 6 mA levels reversely correlate with age; the higher the relative mtDNA 6 mA level, the younger the individual analyzed. Where the value of the relative mtDNA 6 mA level cuts the reference curve, this intersection assigns the corresponding age on the X axis. The reference curve was previously established by determining 6 mA levels at a specific mtDNA site in a large (>1000) number of healthy individuals with known age. The accuracy of the measurement depends on the accuracy of the method by which mtDNA 6 mA level is determined. The method is also suitable for predicting expected lifespan in an individual with known age. The invention relies on our recent biological finding that 6 mA levels at specific mtDNA regions negatively correlate with (is reversely proportional to) the age of the individual analyzed.

The application provides a compound for treating epilepsy and a preparation method thereof. The compound of the application exhibits significant therapeutic effect in the field of epilepsy treatment, has a strong and effective inhibitory effect on the onset of epilepsy, and has extremely excellent therapeutic effect and leads the CBD group in all indexes according to the data analysis of the detection of the expression of apoptosis-related proteins from the evaluation of the molecular biology method. In addition, the compound can effectively prolong the latency of the onset of epilepsy, thereby reducing the frequency of taking medicine and greatly reducing the toxic side effects possibly caused by the medicine. The new compound provided by the application improves the safety and effectiveness of the treatment of epilepsy in many aspects, and opens up a new direction with great potential in the field of epilepsy treatment.

The invention discloses a ZmGIF3 gene for promoting corn grain filling and starch synthesis and application thereof, which can up-regulate expression or activity of ZmGIF3 or homologues thereof in corn, promote corn grain filling capacity, improve corn grain weight, improve corn yield or biomass and improve corn grain starch content. Researches based on methods of genetics, molecular biology and the like find that ZmGIF3.1 and ZmGIF3.2 genes have a positive regulation effect on filling of corn kernels, and when expression of the genes is up-regulated in corn, filling of the corn kernels can be remarkably promoted, the weight of the corn kernels is increased, and the corn kernels become larger; meanwhile, the invention also finds that the ZmGIF3.1 and the ZmGIF3.2 can obviously increase the starch content in the process of regulating and controlling the grain starch. The invention provides a new choice for collaborative improvement of quality and yield of cereal crops.