38 results about "MOLECULAR BIOLOGY METHODS" patented technology

The invention discloses application of an AI-driven target screening technology in anti-obesity treatment of a drug delivery system, and belongs to the field of cross fusion of biological medicine and artificial intelligence. The screening method provided by the invention comprises the following steps: selecting a signal channel target and active small molecules related to lipid metabolism; processing the signal channel target and the active small molecules through the data set to obtain an input matrix; and inputting the input matrix into a deep learning model to obtain the interaction intensity between the active small molecules and the signal path target. According to the method, an artificial intelligence technology is introduced, a deep learning model is constructed to efficiently predict the affinity between a drug and a protein target, the problems that a traditional target screening method depends on experience and is low in efficiency are solved, and target expression is subjected to experimental verification through a molecular biology method, so that the target screening efficiency is improved. A verification closed loop from calculation prediction to molecular demonstration is realized, and the biological credibility of a target screening result is proved.

The invention relates to a method for detecting trophoblast residues in an NK cell product and application of the method. The method comprises the following steps: extracting nucleic acid molecules of the NK cell product, and detecting whether trophoblast specific gene segments exist in the nucleic acid molecules or not. According to the method, the residual condition of the trophoblasts in the NK cell final product is detected by a molecular biological method, specifically, detection can be performed in a manner of amplifying specific genes of the trophoblasts through qPCR or amplifying STR loci of the NK cells through PCR, the method is simple, convenient and rapid, the accuracy rate is high, and the detection precision is improved by combining multiple methods, so that the accuracy of a detection result is ensured.

The invention discloses application of 4-isopropyltoluene to improvement of depression-like behaviors of mice. An experiment adopts a chronic constraint stress method to construct a depression model, through behavioral detection such as a sucrose preference test, a tail suspension test and a novel inhibition ingestion test, and in combination with molecular biology means such as immunofluorescent staining and Western blot, the anti-depression effect and potential mechanism of 4-isopropyltoluene are systematically evaluated, and the anti-depression effect and potential mechanism of 4-isopropyltoluene are evaluated. Results show that 4-isopropyltoluene plays a significant anti-depression role by inhibiting neuroinflammation, enhancing glial cell support function and activating AKT signal pathway, and has the potential of being developed into a novel anti-depression candidate drug.

The invention relates to the technical field of biological medicines, in particular to application of panthenol-cytochrome c reductase core protein 2 lactic acid modification as a therapeutic target in preparation of drugs for treating sepsis cardiomyopathy. The invention discloses a key effect of Uqcrc2 K109 site lactic acid modification in SIC and application of the Uqcrc2 K109 site lactic acid modification as a therapeutic target. The Uqcrc2-K109 is found to be a therapeutic target of the SIC through molecular biology methods such as proteomics analysis, lactylation omics analysis and co-immunoprecipitation, and the function of a mitochondrial respiratory chain complex III is recovered in a targeted manner by regulating and controlling the lactylation level of the Uqcrc2-K109, so that a new strategy is provided for treatment of the SIC.

A molecular biology method, by which relative (normalized to an internal control) N6-methyladenine (6mA) level at a selected specific genomic site in a tissue sample containing numerous individual cellular genomes is accurately determined, and this level is projected to a reference “6mA level-age” curve determined earlier. The relative 6mA level of the examined individual assigns her / his (biological) age on the curve. The reference curve was previously established by determining 6mA levels of a large (>1000) number of healthy individuals with known age. On the reference curve, the relative 6mA level correlates with age; the higher the 6mA level at the tested genomic site, the higher the age of the individual analyzed. The accuracy of the measurement depends on the accuracy of the method by which 6mA level is determined.

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

The invention relates to a specific real-time fluorescent PCR (polymerase chain reaction) amplification primer, a probe and a detection method for identifying lutjanus purpureus, and belongs to the technical field of molecular detection and species identification. According to the invention, the specific primer and the probe for lutjanus purpuratus are designed, and the real-time fluorescent PCR technology is combined, so that the lutjanus purpuratus can be quickly and accurately identified. The method has the advantages of high specificity, high sensitivity, simplicity and convenience in operation and the like, and a reliable molecular biological method is provided for species identification of lutjanus purpurea.

1. The name of the product under this design: Molecular Diagnostic Analyzer. 2. Purpose of this product design: This product design is used in clinical and scientific research fields, and is applied to the detection of molecular biomarkers in samples using molecular biology methods. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the key points of the design: Design 1 Stereoscopic Drawing 1. 5. Designate Design 1 as the base design.

The present invention discloses a strain of Bacillus subtilis and its application, belonging to the technical field of probiotic R & D. In the present invention, a strain of Bacillus subtilis is identified by traditional isolation and culture, morphological, physiological and biochemical, and molecular biological methods, and its probiotic properties are evaluated by in vitro and in vivo tests. The results of in vitro tests show that the strain can tolerate acid (the lowest pH is 2), tolerate salt (the highest NaCl concentration is 10%), is non-hemolytic, inhibits pathogenic bacteria, and is sensitive to most antibiotics; the results of in vivo tests show that adding the strain can improve the production performance of animals, promote the absorption of nutrients by the small intestine, and increase the diversity of dominant intestinal flora. The present invention can not only expand the research on Bacillus subtilis, but also provide a reference basis for the future development of probiotic preparations for antagonizing pathogenic bacteria and regulating the intestinal microecological balance, etc.

The invention discloses a wheat heat resistance related protein TaHSC702 as well as a coding gene and application thereof, the function of the wheat heat resistance related protein TaHSC702 is deeply researched through genetics and molecular biology methods, the effect of the TaHSC702 gene in regulating and controlling the heat resistance of wheat is clarified, two different types of TaHSC702 mutants are created through EMS mutagenesis, and the wheat heat resistance related protein TaHSC702 is obtained. The two mutant proteins can significantly improve the heat resistance of wheat. The important significance is realized on the breeding of the heat-resistant wheat.

The application discloses a pangolin species identification method based on nail piece element characteristics, which comprises the following steps: step S1: collecting nail piece samples covering existing 10 kinds of pangolin species, and obtaining initial data of multi-element characteristics of the nail piece samples; step S2: identifying the species of the nail piece samples by a molecular biology method, and obtaining the species labels of each nail piece sample; step S3: constructing an "element characteristic-species label" standard data set; step S4: selecting a table prior fitting network as a core machine learning algorithm, training a model by using training set data, and constructing the model; and step S5: identifying whether a to-be-detected sample belongs to a pangolin nail piece according to morphological judgment. The method has a single sample data collection time of less than 60 seconds, does not need to damage the sample, is used for nail piece identification of samples without prior clues, and the overall accuracy of a global ten-species classification model reaches 0.93.

The invention relates to the technical field of medicines, in particular to application of DNMT3L gene coded protein in preparation of antiviral drugs, the DNMT3L coding gene is proved to have the effect of inhibiting replication and transcription of linear double-stranded DNA viruses or single-stranded negative-stranded RNA viruses, and analysis shows that cells over-expressing the DNMT3L coding gene can promote interferon expression, so that the anti-virus effect of the DNMT3L coding gene is improved, and the anti-virus effect of the DNMT3L coding gene is improved. According to the present invention, the DNMT3L coding gene is constructed into the overexpression plasmid by using the molecular biology method so as to significantly inhibit the replication of the linear double-chain DNA virus or the single-strand negative-chain RNA virus, and the DNMT3L coding gene is overexpressed in the host cell by using the vector so as to enhance the cell antiviral ability;

The invention discloses a primer and a kit for detecting a citrus broken leaf virus based on RPA-CRISPR-Cas12b and application of the primer and the kit, and relates to the technical field of citrus pathogen detection. The invention develops an isothermal amplification detection method based on RPA and CRISPR for common CTLV pathogen targets in citrus, gets rid of the dependence of a traditional molecular biological method on a complex instrument and equipment platform, and greatly simplifies a detection platform; through system establishment and verification, the total detection time of the CTLV target is shortened to be within half an hour; meanwhile, a verification test result shows that the detection method developed by the invention has relatively good sensitivity and specificity, and an actual sample verification result is consistent with a result of an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method.

A novel circRNA, its uses, and a reagent kit are disclosed, relating to the field of animal genetics and breeding. The nucleotide sequence of Circ-06958 of this invention is shown in SEQ ID NO.1 of the sequence listing. Circ-06958 is used in the preparation of a reagent for the proliferation of porcine skeletal muscle satellite cells. This invention successfully cloned and identified a novel circRNA sequence—Circ-06958. Molecular biological methods confirmed that Circ-06958 is a marker for the proliferation of porcine skeletal muscle satellite cells, and increasing the expression level of Circ-06958 can promote the proliferation of porcine skeletal muscle satellite cells.

The invention discloses a wheat heat resistance related protein TaHSC701 as well as an encoding gene and application thereof, the function of the wheat heat resistance related protein TaHSC701 is deeply researched through genetics and molecular biology methods, the effect of the TaHSC701 gene in regulating and controlling the heat resistance of wheat is defined, and the wheat heat resistance related protein TaHSC701 has important significance in breeding of heat-resistant wheat.

The invention relates to the technical field of bioinformatics, in particular to a method for detecting biodiversity based on eDNA metagenomes and macro bar codes. Comprising the following steps: collecting a sediment sample in a monitoring area and extracting eDNA; carrying out metagenome high-throughput sequencing on the extracted eDNA to obtain a metagenome original sequence; performing quality control on the metagenome original sequence to obtain a metagenome quality control sequence; assembling according to the repeated fragments to obtain a metagenome long sequence; and according to the specific marker gene, screening out a query sequence and carrying out annotation according to the query sequence. Compared with a traditional ecological system field investigation method, a molecular biology method is applied, investigation and monitoring of biological diversity are more efficient, more accurate and more comprehensive, and interference to the ecological system and organisms is small.

The present invention discloses a specific primer for identifying Herba Chrysanthemi Morifolii from Bozhou by a molecular biology method and a method for identifying Herba Chrysanthemi Morifolii from Bozhou, belonging to the technical field of molecular identification. By widely investigating the information of the main cultivation areas of Herba Chrysanthemi Morifolii from Bozhou, the co-distributed species in the wild and the related medicinal species, and then sampling as many varieties as possible, and then through comparison, the specific information sites of the ITS sequence unique to Herba Chrysanthemi Morifolii from Bozhou are found, and further specific primers are designed: the specific primers are SEQ ID NO.1 and SEQ ID NO.2, and the use of the specific primers will greatly facilitate the rapid identification of the Chinese medicinal material Herba Chrysanthemi Morifolii from Bozhou.

The invention belongs to the technical field of microbiological detection, and particularly relates to a method for rapidly detecting viable salmonella and application. The method comprises the following steps: mixing a sample to be detected with Felix O-1 bacteriophage, incubating at 37 DEG C to enable the Felix O-1 bacteriophage to infect and proliferate in the sample to be detected, and performing splitting decomposition treatment after infection proliferation is finished to obtain proliferated Felix O-1 bacteriophage DNA (Deoxyribose Nucleic Acid); felix O-1 bacteriophage DNA is used as a template for real-time fluorescence RPA amplification, and whether viable salmonella exists or not is judged through fluorescence signal intensity. According to the method provided by the invention, the defects that the traditional bacterial culture and biochemical confirmation method is long in time consumption and the original molecular biological method cannot conveniently distinguish dead and live are overcome. The method is short in detection time, the RPA reaction is carried out under a constant-temperature condition, equipment requirements are simple, and the method is suitable for field detection.

The application of Circ-21490 in proliferation of pig skeletal muscle satellite cells relates to the field of molecular genetics, the application of circRNA in proliferation of pig skeletal muscle satellite cells, the circRNA is Circ-21490, the nucleotide sequence of Circ-21490 is shown as SEQ ID No:1.The application successfully clones and identifies a new circRNA sequence-Circ-21490, determines that Circ-21490 is a marker for proliferation of pig skeletal muscle satellite cells through a molecular biology method, and overexpression of Circ-21490 can promote proliferation of pig skeletal muscle satellite cells.A detection kit containing a specific primer pair of Circ-21490 is designed, the expression level of Circ-21490 is analyzed by using the kit, so as to identify the proliferation potential of pig skeletal muscle satellite cells.

The present invention belongs to the field of biotechnology, and particularly relates to a genetic transformation method for gene knockout in Cordyceps cicadae mediated by Agrobacterium tumefaciens. The present invention discloses the construction of a knockout vector for a target gene beas, and also simultaneously discloses a method for knocking out genes in Cordyceps cicadae by Agrobacterium tumefaciens mediation. The present invention uses molecular biological methods to knock out the key synthetic gene of beauvericin in Cordyceps cicadae fungi, so that beauvericin is not expressed or has a very low content in the mycelium harvested after culturing the strain for 24 days. The strain after knocking out the key synthetic gene of beauvericin has genetic stability of not producing beauvericin. It provides a basis for the development of new drugs for Cordyceps cicadae fungi that do not produce beauvericin.

The invention discloses a mitochondrial cox gene segment for detecting Cailanthus splendens, application of the mitochondrial cox gene segment and a method for detecting leaf spot of oak trees. According to the present invention, a strain of Ciocereus ciliatus LDCQJJ4 from a oak leaf spot disease sample is obtained, the preservation number of the strain is GDMCC No.3.1250, the specific fragment of the mitochondrial cox gene of the strain is obtained through research, and the nucleotide sequence of the specific fragment is represented by SEQ ID NO.5, such that the specific fragment can be used for the specific detection of the pathogenic bacteria so as to achieve the specific detection and diagnosis of the oak leaf spot caused by the Ciocereus ciliatus LDCQJJ4, and the specific detection and the diagnosis of the oak leaf spot caused by the Ciocereus ciliatus LDCQJJ4 can be provided for the oak leaf spot caused by the Ciocereus ciliatus LDCQJJ4. The invention further provides a specific primer and a kit aiming at the gene segment, and the primer is high in specificity and sensitivity. According to the method, a molecular biology means is used for detecting the ailanthus slaflock bacillus megatherium for the first time, the detection method is specific, sensitive, rapid, simple and convenient, results are reliable, diseases can be found as early as possible, corresponding prevention and control measures can be taken in time, and the influence of leaf spot on the yield and quality of oak trees is reduced.

The invention discloses a Wikstrolide E mediated psoriasis animal model and a construction method thereof, and belongs to the technical field of animal models. Aiming at the problem that the application of the existing psoriasis model is limited, a novel psoriasis animal model is constructed by utilizing a compound Wikstrolide E, and psoriasis characteristics which can be simulated by the animal model are evaluated through PASI scoring, histopathology, dermoscopy and a molecular biological method. The model is expected to become an extremely valuable tool in the fields of psoriasis research and drug discovery.

This invention relates to the fields of microbial technology and animal pathogen control technology, specifically to an endophytic fungal extract and its application against *Glassonella parasuis*. The specific technical solution is as follows: the endophytic fungal strain is derived from *Fritillaria cirrhosa* from Hubei Province, identified as a *Talaromyces* fungus by molecular biological methods, and deposited at the China Center for Type Culture Collection (CCTCC). Studies have shown that the fermentation product of this strain and the extract obtained by ethyl acetate extraction are effective against *Glassonella parasuis* (…). Glaesserella parasuis It exhibits significant inhibitory effects, effectively suppressing its growth and reducing its pathogenic risk. The endophytic fungal strains and their extracts described in this invention can be used to prepare antibacterial agents against *Glassonella parasuis*, showing promising application prospects in the field of livestock disease control.

The present invention discloses a female-specific primer for Shinisaurus crocodilurus and a method for sex identification. Genomic DNA of the Shinisaurus crocodilurus to be tested is extracted, and then amplified with the above-mentioned specific primer. After detection by agarose gel electrophoresis, if there is a specific band at 557 bp, it indicates that the sample corresponds to a female Shinisaurus crocodilurus; if there is no band, it is a male Shinisaurus crocodilurus. Since Shinisaurus crocodilurus has no obvious secondary sexual characteristics, there is a lack of a reliable method for identifying the sex of Shinisaurus crocodilurus in the prior art, especially for juvenile Shinisaurus crocodilurus, it is impossible to determine the sex. The method adopted in the present invention is a molecular biology method based on genomic information, and is detected by PCR method, which has the advantages of high repeatability, strong reliability, high throughput, and independence from the experience of breeders. Whether it is an adult or a juvenile Shinisaurus crocodilurus, its sex can be accurately determined.

The application discloses application of MST1 interfering RNA in promoting in-vitro differentiation of ESCs into insulin-secreting cells, and belongs to the technical field of biotechnology. In the process of in-vitro directional differentiation of rat ESCs into beta cells, the application infects by using an MST1 shRNA lentivirus vector, and indexes are detected and functions are determined by using cell biology and molecular biology methods, and it is found for the first time that the efficiency of inducing ESCs to differentiate into beta cell-like cells can be improved by reducing the expression of MST1. Meanwhile, how MST1 realizes the regulation of beta cell maturation by means of PDX1 in the process of directional differentiation of rat ESCs into beta cells is described, the reason affecting in-vitro maturation of beta cells is discussed, and thus the system of directional differentiation of ESCs into mature beta cells is optimized, which has important significance for obtaining beta cells with normal insulin secretion function in-vitro.

The invention discloses a filling gene for increasing corn kernel weight and application thereof, expression or activity of ZmMYB29 or homologues thereof is up-regulated in corn, and corn characters can be improved: (i) filling of corn kernels is promoted; (ii) increasing the weight of the corn kernels; (iii) increasing the yield or biomass of corn; (iv) the starch content in the corn kernels is increased. Researches based on methods of genetics, molecular biology and the like find that the ZmMYB29 gene has a positive regulation effect on filling of corn kernels, and when expression of the gene is up-regulated in corn, filling of the corn kernels can be remarkably promoted, the weight of the corn kernels is increased, and the corn kernels become larger; meanwhile, the ZmMYB29 also finds that the starch content can be obviously increased in the process of regulating and controlling the grain starch by the ZmMYB29. The invention provides a new choice for collaborative improvement of quality and yield of cereal crops.

Vibrio parahaemolyticus serotype O10 is an important foodborne pathogen with a very high isolation rate in my country in recent years. This invention provides a specific oligonucleotide sequence for detecting Vibrio parahaemolyticus serotype O10 and its application. The oligonucleotides are DNA fragments selected from the IS1 and orf9 genes of the O antigen gene cluster of Vibrio parahaemolyticus serotype O10. This invention also establishes a PCR detection method for Vibrio parahaemolyticus serotype O10 using the above-mentioned oligonucleotides, establishing for the first time a technique for detecting this bacterium using molecular biology methods. This is of great significance for the identification and epidemiological monitoring of this important foodborne pathogen.

This invention discloses a molecular biology method and its application for simultaneously detecting five poultry-derived components. Using primers shown in SEQ ID NO.1 and SEQ ID NO.2, genomic DNA of the sample to be tested is amplified by PCR. After capillary electrophoresis, sequence alignment is performed. A peak value of 234 bp indicates a chicken-derived component; a peak value of 271 bp indicates a quail-derived component; a peak value of 229 bp indicates a turkey-derived component; a peak value of 252 bp indicates a partridge-derived component; and a peak value of 223 bp indicates a pheasant-derived component. Peak values ​​of 234 bp, 271 bp, 229 bp, 252 bp, and 223 bp indicate the simultaneous presence of five poultry-derived components. The detection kit developed based on this method can generate considerable economic benefits and good social value.

The present invention belongs to the technical field of animal models, and particularly relates to the application of inhibitors in improving placental hypertrophy in cloned animals. This method involves grouping mice, superovulating, and co-housing them for pregnancy to obtain pregnant mice. By replacing the drinking water with α-ketoglutaric acid and sodium crotonate, and adding gene Idh2 inhibitors and IDH1 inhibitors, a mouse model of placental hypertrophy is constructed. On the 16th day of mouse pregnancy, the mouse fetuses and placentas are dissected and separated, and the sizes of the mouse fetuses, the sizes of the placentas, and the weights of the placentas are observed and recorded. Without using molecular biology methods, this method constructs a mouse model of placental hypertrophy through drug induction, and by adding inhibitors of key genes, the purpose of reducing the hypertrophic placenta is achieved, solving the problems of long cycle, low efficiency, and high cost in reducing the hypertrophic placenta of cloned animals by using gene editing methods at present.

The invention belongs to the technical field of insect resistance research, and discloses a method for enhancing insect resistance of poplar by using a leaf aging related gene NAC109, which is characterized by comprising the following steps: (1) obtaining a CDS sequence of a PtNAC109 gene: (2) designing a guide gRNA sequence; (3) constructing a targeted PtNAC109 gene editing carrier plasmid; (4) preparing a genetic transformation agrobacterium infection solution; (5) infecting the populus tomentosa plants with agrobacterium; (6) screening calluses with resistance; (7) inducing, prolonging and rooting resistant buds; (8) verifying a gene editing effect by utilizing a molecular biology means; and (9) obtaining a poplar gene editing new germplasm with enhanced insect resistance.