61 results about "MOLECULAR BIOLOGY METHODS" patented technology

The invention discloses a molecular biological method capable of simultaneously detecting duck-derived and goose-derived components and application of the molecular biological method, after genome DNA of a sample to be detected is subjected to PCR amplification and capillary electrophoresis through primers shown in SEQ ID NO.1 and SEQ ID NO.2, sequence comparison is carried out, when the peak value is 999bp, it is indicated that the sample contains the duck-derived component, and when the peak value is 992bp, it is indicated that the sample contains the goose-derived component, and when the peak value is 992bp, it is indicated that the sample contains the goose-derived component, it is indicated that the sample contains the duck-derived component. When the peak values are 999bp and 992bp, the duck-derived component and the goose-derived component are contained at the same time. The detection kit developed on the basis of the method can generate considerable economic benefits and good social values.

The invention discloses application of an AI-driven target screening technology in anti-obesity treatment of a drug delivery system, and belongs to the field of cross fusion of biological medicine and artificial intelligence. The screening method provided by the invention comprises the following steps: selecting a signal channel target and active small molecules related to lipid metabolism; processing the signal channel target and the active small molecules through the data set to obtain an input matrix; and inputting the input matrix into a deep learning model to obtain the interaction intensity between the active small molecules and the signal path target. According to the method, an artificial intelligence technology is introduced, a deep learning model is constructed to efficiently predict the affinity between a drug and a protein target, the problems that a traditional target screening method depends on experience and is low in efficiency are solved, and target expression is subjected to experimental verification through a molecular biology method, so that the target screening efficiency is improved. A verification closed loop from calculation prediction to molecular demonstration is realized, and the biological credibility of a target screening result is proved.

The invention discloses a bacillus licheniformis strain and application thereof. Belongs to the technical field of probiotics. A strain of bacillus licheniformis is separated and identified through a traditional identification method and a molecular biological method, and the bacillus licheniformis is subjected to probiotic performance evaluation and animal in-vivo safety evaluation. Meanwhile, microbiology is combined with pathology and the like, so that mouse organ lesions caused by salmonella can be relieved by drenching the bacterium, and an antagonistic effect on salmonella in a mouse body can be generated. According to the invention, not only can the research on bacillus be expanded, but also a reference basis can be provided for developing and researching probiotic microecological preparations for promoting digestion and growth of animals, antagonizing pathogenic bacteria, regulating intestinal microecological environment and the like in the future.

The invention provides an additive formula optimization method for improving the effect of a suckercide based on computational chemistry, and belongs to the technical field of suckercide, and the method comprises the following steps: obtaining key components in a mixed ester alcohol suckercide, and determining an optimal combination through suckercide effect evaluation; the cell structure change of tobacco axillary bud meristem treated by the bud inhibitors with different concentrations is observed by adopting an electron microscope and a biological slicing technology; analyzing the influence of the suckercide on the gene regulation network in the early development stage of the tobacco axillary buds by utilizing multi-omics analysis, and identifying a core regulation gene; a molecular biology method is used for verifying the function of the key gene, and a bud inhibition mechanism is analyzed; based on a multi-objective optimization model, an optimal auxiliary agent formula is determined, and the comprehensive performance is evaluated in a small-scale test. The research is helpful for developing a new efficient, low-cost and environment-friendly tobacco bud inhibition technology. The technical problems that an existing additive formula optimization method depends on experience, and a formula comprehensively optimized in multiple aspects of effect, risk, stability and the like is difficult to find are solved.

The invention relates to a method for detecting trophoblast residues in an NK cell product and application of the method. The method comprises the following steps: extracting nucleic acid molecules of the NK cell product, and detecting whether trophoblast specific gene segments exist in the nucleic acid molecules or not. According to the method, the residual condition of the trophoblasts in the NK cell final product is detected by a molecular biological method, specifically, detection can be performed in a manner of amplifying specific genes of the trophoblasts through qPCR or amplifying STR loci of the NK cells through PCR, the method is simple, convenient and rapid, the accuracy rate is high, and the detection precision is improved by combining multiple methods, so that the accuracy of a detection result is ensured.

The invention discloses application of 4-isopropyltoluene to improvement of depression-like behaviors of mice. An experiment adopts a chronic constraint stress method to construct a depression model, through behavioral detection such as a sucrose preference test, a tail suspension test and a novel inhibition ingestion test, and in combination with molecular biology means such as immunofluorescent staining and Western blot, the anti-depression effect and potential mechanism of 4-isopropyltoluene are systematically evaluated, and the anti-depression effect and potential mechanism of 4-isopropyltoluene are evaluated. Results show that 4-isopropyltoluene plays a significant anti-depression role by inhibiting neuroinflammation, enhancing glial cell support function and activating AKT signal pathway, and has the potential of being developed into a novel anti-depression candidate drug.

The invention relates to the technical field of biological medicines, in particular to application of panthenol-cytochrome c reductase core protein 2 lactic acid modification as a therapeutic target in preparation of drugs for treating sepsis cardiomyopathy. The invention discloses a key effect of Uqcrc2 K109 site lactic acid modification in SIC and application of the Uqcrc2 K109 site lactic acid modification as a therapeutic target. The Uqcrc2-K109 is found to be a therapeutic target of the SIC through molecular biology methods such as proteomics analysis, lactylation omics analysis and co-immunoprecipitation, and the function of a mitochondrial respiratory chain complex III is recovered in a targeted manner by regulating and controlling the lactylation level of the Uqcrc2-K109, so that a new strategy is provided for treatment of the SIC.

A molecular biology method, by which relative (normalized to an internal control) N6-methyladenine (6mA) level at a selected specific genomic site in a tissue sample containing numerous individual cellular genomes is accurately determined, and this level is projected to a reference “6mA level-age” curve determined earlier. The relative 6mA level of the examined individual assigns her / his (biological) age on the curve. The reference curve was previously established by determining 6mA levels of a large (>1000) number of healthy individuals with known age. On the reference curve, the relative 6mA level correlates with age; the higher the 6mA level at the tested genomic site, the higher the age of the individual analyzed. The accuracy of the measurement depends on the accuracy of the method by which 6mA level is determined.

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

The invention relates to a specific real-time fluorescent PCR (polymerase chain reaction) amplification primer, a probe and a detection method for identifying lutjanus purpureus, and belongs to the technical field of molecular detection and species identification. According to the invention, the specific primer and the probe for lutjanus purpuratus are designed, and the real-time fluorescent PCR technology is combined, so that the lutjanus purpuratus can be quickly and accurately identified. The method has the advantages of high specificity, high sensitivity, simplicity and convenience in operation and the like, and a reliable molecular biological method is provided for species identification of lutjanus purpurea.

1. The name of the product under this design: Molecular Diagnostic Analyzer. 2. Purpose of this product design: This product design is used in clinical and scientific research fields, and is applied to the detection of molecular biomarkers in samples using molecular biology methods. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the key points of the design: Design 1 Stereoscopic Drawing 1. 5. Designate Design 1 as the base design.

The present invention discloses a strain of Bacillus subtilis and its application, belonging to the technical field of probiotic R & D. In the present invention, a strain of Bacillus subtilis is identified by traditional isolation and culture, morphological, physiological and biochemical, and molecular biological methods, and its probiotic properties are evaluated by in vitro and in vivo tests. The results of in vitro tests show that the strain can tolerate acid (the lowest pH is 2), tolerate salt (the highest NaCl concentration is 10%), is non-hemolytic, inhibits pathogenic bacteria, and is sensitive to most antibiotics; the results of in vivo tests show that adding the strain can improve the production performance of animals, promote the absorption of nutrients by the small intestine, and increase the diversity of dominant intestinal flora. The present invention can not only expand the research on Bacillus subtilis, but also provide a reference basis for the future development of probiotic preparations for antagonizing pathogenic bacteria and regulating the intestinal microecological balance, etc.

The invention discloses a wheat heat resistance related protein TaHSC702 as well as a coding gene and application thereof, the function of the wheat heat resistance related protein TaHSC702 is deeply researched through genetics and molecular biology methods, the effect of the TaHSC702 gene in regulating and controlling the heat resistance of wheat is clarified, two different types of TaHSC702 mutants are created through EMS mutagenesis, and the wheat heat resistance related protein TaHSC702 is obtained. The two mutant proteins can significantly improve the heat resistance of wheat. The important significance is realized on the breeding of the heat-resistant wheat.

The application discloses a pangolin species identification method based on nail piece element characteristics, which comprises the following steps: step S1: collecting nail piece samples covering existing 10 kinds of pangolin species, and obtaining initial data of multi-element characteristics of the nail piece samples; step S2: identifying the species of the nail piece samples by a molecular biology method, and obtaining the species labels of each nail piece sample; step S3: constructing an "element characteristic-species label" standard data set; step S4: selecting a table prior fitting network as a core machine learning algorithm, training a model by using training set data, and constructing the model; and step S5: identifying whether a to-be-detected sample belongs to a pangolin nail piece according to morphological judgment. The method has a single sample data collection time of less than 60 seconds, does not need to damage the sample, is used for nail piece identification of samples without prior clues, and the overall accuracy of a global ten-species classification model reaches 0.93.

The present invention relates to a DNA probe, a kit and a method for distinguishing between hyacinthus annuus and water hyacinth, wherein the DNA probe sequence is the nucleotide sequence shown in SEQ.ID.NO.1. According to the alignment results of the chloroplast genomes of hyacinthus annuus and water hyacinth, the present application found that there is a differential site in the 11 kb region. Through this site, we can distinguish between hyacinthus annuus and water hyacinth, which is an ideal site for molecular markers. Primers are designed based on this, and molecular biological methods such as RPA (Recombinase Polymerase Amplification) can be used to detect leaf tissues of hyacinthus annuus and water hyacinth. Molecular identification can be achieved by the presence or absence of fluorescent signals, so that hyacinthus annuus and water hyacinth can be distinguished reliably and effectively.

The invention discloses a novel circRNA (Ribonucleic Acid), application and a kit, and relates to the field of animal genetic breeding, and the nucleotide sequence of the circ-06958 is shown as SEQ ID NO.1 in a sequence table. The invention discloses application of Circ-06958 in preparation of a porcine skeletal muscle satellite cell proliferation reagent. According to the invention, a new circRNA sequence-CircRNA is successfully cloned and identified, the circ-06958 is determined to be the porcine skeletal muscle satellite cell proliferation marker through a molecular biological method, and the porcine skeletal muscle satellite cell proliferation can be promoted by increasing the expression quantity of the circ-06958.

The invention relates to the technical field of medicines, in particular to application of DNMT3L gene coded protein in preparation of antiviral drugs, the DNMT3L coding gene is proved to have the effect of inhibiting replication and transcription of linear double-stranded DNA viruses or single-stranded negative-stranded RNA viruses, and analysis shows that cells over-expressing the DNMT3L coding gene can promote interferon expression, so that the anti-virus effect of the DNMT3L coding gene is improved, and the anti-virus effect of the DNMT3L coding gene is improved. According to the present invention, the DNMT3L coding gene is constructed into the overexpression plasmid by using the molecular biology method so as to significantly inhibit the replication of the linear double-chain DNA virus or the single-strand negative-chain RNA virus, and the DNMT3L coding gene is overexpressed in the host cell by using the vector so as to enhance the cell antiviral ability;

The invention discloses a molecular marker related to the seed setting rate of broccoli seeds and its application. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1. The 51st position of the sequence has a C / T polymorphism. The SNP is located at the 37728588th position of the C6 chromosome of the broccoli HDEM (verson 1.0) reference genome. When the base sequence mutates from C to T, the corresponding broccoli seed setting rate is improved. The SNP molecular marker is used to screen broccoli germplasm with a high seed setting rate. The KASP primer is used for PCR amplification. The primer excites HEX fluorescence in broccoli materials with a low seed setting rate, and the SNP excites FAM fluorescence in broccoli materials with a high seed setting rate, thereby realizing early and rapid identification of broccoli germplasm resources by molecular biological means.

The invention discloses a primer and a kit for detecting a citrus broken leaf virus based on RPA-CRISPR-Cas12b and application of the primer and the kit, and relates to the technical field of citrus pathogen detection. The invention develops an isothermal amplification detection method based on RPA and CRISPR for common CTLV pathogen targets in citrus, gets rid of the dependence of a traditional molecular biological method on a complex instrument and equipment platform, and greatly simplifies a detection platform; through system establishment and verification, the total detection time of the CTLV target is shortened to be within half an hour; meanwhile, a verification test result shows that the detection method developed by the invention has relatively good sensitivity and specificity, and an actual sample verification result is consistent with a result of an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method.

A novel circRNA, its uses, and a reagent kit are disclosed, relating to the field of animal genetics and breeding. The nucleotide sequence of Circ-06958 of this invention is shown in SEQ ID NO.1 of the sequence listing. Circ-06958 is used in the preparation of a reagent for the proliferation of porcine skeletal muscle satellite cells. This invention successfully cloned and identified a novel circRNA sequence—Circ-06958. Molecular biological methods confirmed that Circ-06958 is a marker for the proliferation of porcine skeletal muscle satellite cells, and increasing the expression level of Circ-06958 can promote the proliferation of porcine skeletal muscle satellite cells.

The invention discloses a wheat heat resistance related protein TaHSC701 as well as an encoding gene and application thereof, the function of the wheat heat resistance related protein TaHSC701 is deeply researched through genetics and molecular biology methods, the effect of the TaHSC701 gene in regulating and controlling the heat resistance of wheat is defined, and the wheat heat resistance related protein TaHSC701 has important significance in breeding of heat-resistant wheat.

The invention relates to the technical field of bioinformatics, in particular to a method for detecting biodiversity based on eDNA metagenomes and macro bar codes. Comprising the following steps: collecting a sediment sample in a monitoring area and extracting eDNA; carrying out metagenome high-throughput sequencing on the extracted eDNA to obtain a metagenome original sequence; performing quality control on the metagenome original sequence to obtain a metagenome quality control sequence; assembling according to the repeated fragments to obtain a metagenome long sequence; and according to the specific marker gene, screening out a query sequence and carrying out annotation according to the query sequence. Compared with a traditional ecological system field investigation method, a molecular biology method is applied, investigation and monitoring of biological diversity are more efficient, more accurate and more comprehensive, and interference to the ecological system and organisms is small.

The present invention discloses a specific primer for identifying Herba Chrysanthemi Morifolii from Bozhou by a molecular biology method and a method for identifying Herba Chrysanthemi Morifolii from Bozhou, belonging to the technical field of molecular identification. By widely investigating the information of the main cultivation areas of Herba Chrysanthemi Morifolii from Bozhou, the co-distributed species in the wild and the related medicinal species, and then sampling as many varieties as possible, and then through comparison, the specific information sites of the ITS sequence unique to Herba Chrysanthemi Morifolii from Bozhou are found, and further specific primers are designed: the specific primers are SEQ ID NO.1 and SEQ ID NO.2, and the use of the specific primers will greatly facilitate the rapid identification of the Chinese medicinal material Herba Chrysanthemi Morifolii from Bozhou.

The invention discloses a soybean disease-resistant and insect-resistant molecular marker-assisted breeding method. The method comprises the following steps: collecting and storing soybean germplasm resources with different disease-resistant and insect-resistant characters; the method comprises the following steps: screening a molecular marker closely linked with disease and pest resistance characters from a soybean genome by using a genome sequencing method; based on the screened molecular marker, constructing a soybean genetic map, and defining a linkage relationship between the molecular marker and a target disease and pest resistant gene; detecting the genotype of the molecular marker in a soybean material to be bred through a molecular biological method, carrying out auxiliary selection according to the linkage relationship between the molecular marker and the target disease and pest resistant gene, and screening out soybeans carrying the target disease and pest resistant gene; soybean materials which are excellent in genetic background and carry target disease and pest resistant genes are selected as parents to carry out cross combination, and through multi-generation backcross and selfing, the genetic background is further purified, and target characters are stabilized. The method can greatly shorten the breeding period and improve the breeding efficiency.

The invention belongs to the technical field of microbiological detection, and particularly relates to a method for rapidly detecting viable salmonella and application. The method comprises the following steps: mixing a sample to be detected with Felix O-1 bacteriophage, incubating at 37 DEG C to enable the Felix O-1 bacteriophage to infect and proliferate in the sample to be detected, and performing splitting decomposition treatment after infection proliferation is finished to obtain proliferated Felix O-1 bacteriophage DNA (Deoxyribose Nucleic Acid); felix O-1 bacteriophage DNA is used as a template for real-time fluorescence RPA amplification, and whether viable salmonella exists or not is judged through fluorescence signal intensity. According to the method provided by the invention, the defects that the traditional bacterial culture and biochemical confirmation method is long in time consumption and the original molecular biological method cannot conveniently distinguish dead and live are overcome. The method is short in detection time, the RPA reaction is carried out under a constant-temperature condition, equipment requirements are simple, and the method is suitable for field detection.

The application of Circ-21490 in proliferation of pig skeletal muscle satellite cells relates to the field of molecular genetics, the application of circRNA in proliferation of pig skeletal muscle satellite cells, the circRNA is Circ-21490, the nucleotide sequence of Circ-21490 is shown as SEQ ID No:1.The application successfully clones and identifies a new circRNA sequence-Circ-21490, determines that Circ-21490 is a marker for proliferation of pig skeletal muscle satellite cells through a molecular biology method, and overexpression of Circ-21490 can promote proliferation of pig skeletal muscle satellite cells.A detection kit containing a specific primer pair of Circ-21490 is designed, the expression level of Circ-21490 is analyzed by using the kit, so as to identify the proliferation potential of pig skeletal muscle satellite cells.

The present invention belongs to the field of biotechnology, and particularly relates to a genetic transformation method for gene knockout in Cordyceps cicadae mediated by Agrobacterium tumefaciens. The present invention discloses the construction of a knockout vector for a target gene beas, and also simultaneously discloses a method for knocking out genes in Cordyceps cicadae by Agrobacterium tumefaciens mediation. The present invention uses molecular biological methods to knock out the key synthetic gene of beauvericin in Cordyceps cicadae fungi, so that beauvericin is not expressed or has a very low content in the mycelium harvested after culturing the strain for 24 days. The strain after knocking out the key synthetic gene of beauvericin has genetic stability of not producing beauvericin. It provides a basis for the development of new drugs for Cordyceps cicadae fungi that do not produce beauvericin.

The present invention relates to a human cytomegalovirus (HCMV) diagnosis method based on recombinase polymerase amplification and CRISPR-Cas12a. It has been identified in the present invention that the HCMV diagnosis method using a CRISPR-Cas12a system and a recombinase polymerase amplification primer, which specifically binds to the genome sequence of the essential envelope glycoprotein H of human cytomegalovirus (HCMV), and can accurately detect only HCMV from among similar viruses. In addition, it has been identified that the present invention can diagnose HCMV infection in an actual clinical sample, and has excellent sensitivity and specificity even in comparison to a conventional molecular biological method, and thus can be used for rapid and accurate point-of-care testing of HCMV.

The invention relates to a diagnostic kit for detecting preeclampsia by a qPCR (quantitative polymerase chain reaction) method, which is characterized in that a preeclampsia biomarker is PPAP-A, a detection primer group is provided according to a gene sequence of the PPAP-A and a human housekeeping gene beta-actin sequence, a forward primer of the PPAP-A is shown as SEQ ID NO.1, a reverse primer of the PPAP-A is shown as SEQ ID NO.2, a reverse primer of the PPAP-A is shown as SEQ ID NO.3, and a reverse primer of the PPAP-A is shown as SEQ ID NO.4. A reverse primer of the PPAP-A is as shown in SEQ ID NO. 2; a fluorescent probe of the PPAP-A is as shown in SEQ ID NO. 3; a forward primer of beta-actin is as shown in SEQ ID NO. 4; a reverse primer of the beta-actin is as shown in SEQ ID NO. 5; and a fluorescent probe of beta-actin is as shown in SEQ ID NO. 6. According to the invention, a molecular biology method is innovatively adopted to diagnose preeclampsia of the pregnant woman, so that a basis is provided for early intervention and customized treatment, and early risk assessment and intervention in an early pregnancy period become possible.

The invention discloses a leiocassis longirostris parent fish gender identification method based on image color reversal. The method comprises the following steps: S1, selecting and fixing leiocassis longirostris parent fish needing gender identification; s2, identifying the gender of the parent fish through a gender identification system; s3, re-identifying the parent fish of which the sex is not determined; s4, performing male and female classification on the parent fish with the determined gender; the defects of the existing identification method are overcome, compared with external morphological observation, the method is more accurate and has no destructiveness of internal dissection, the cost is lower than that of a molecular biological method, and the equipment cost is lower than that of an ultrasonic technology; according to the leiocassis longirostris sex identification system, the genital foramen features of parent fishes are accurately extracted through image acquisition, image preprocessing, image inversion, feature extraction and classification identification modules in the sex identification system, then sex identification is carried out, the parent fishes of which the sex is determined are classified and marked, subsequent management and breeding are facilitated, and the leiocassis longirostris genetic breeding and breeding efficiency is improved.