Screening method and application of Dongxiang blue-eggshell chicken genome SNP molecular marker

A screening method, the technology of green-shell layer hens, applied in the field of genetics, can solve the problems of poor accuracy, limited amount of genetic variation information in the genome, and restrictions on the development and utilization of Dongxiang green-shell layer hen breed resources, so as to facilitate development, utilization, and detection The effect of high precision and rich data volume

Pending Publication Date: 2019-02-01
JIANGSU INST OF POULTRY SCI
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AI Technical Summary

Problems solved by technology

At present, microsatellite markers and mitochondrial DNA sequence variation have been widely used in the analysis of genetic diversity, genetic structure and origin of local chicken breeds (including Dongxiang green-shell layer hens), but the coverage of these DNA markers in the whole chicken genome is Extremely small, for example, there are only about 30 microsatellite markers in chicken, and the D-loop region of chicken mitochondrial DNA is only about 1200bp in length, which represents a very limited amount of genetic variation information in the genome
At present, the evaluation of the conservation effect of Dongxiang green-shell hens mainly depends on conventional phenotypic trait records, pedigree records and low-density DNA molecular marker information. The throughput is low and the accuracy is poor, so it cannot be objectively evaluated. What is the effect of this species; it also limits the development and utilization of Dongxiang green-shell layer hen breed resources

Method used

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  • Screening method and application of Dongxiang blue-eggshell chicken genome SNP molecular marker
  • Screening method and application of Dongxiang blue-eggshell chicken genome SNP molecular marker
  • Screening method and application of Dongxiang blue-eggshell chicken genome SNP molecular marker

Examples

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Embodiment 1

[0048] Example 1 Screening of Genomic SNP Molecular Markers of Dongxiang Green-shell Hens

[0049] (a) Blood sample collection: 18 local chicken breeds (Langshan Chicken, Anyiwa Gray Chicken, Gushi Chicken, Xianju Chicken, White-eared Yellow Chicken, Henan Gamecock, Jinhu Wufeng Chicken, Wenchang Chicken, Daweishan Micro Chicken, Tibetan Chicken, Camellia Chicken, Piao Chicken, Huiyang Bearded Chicken, Big Bone Chicken, Xiaoshan Chicken, Luyuan Chicken, Bian Chicken and Beijing You Chicken) and 2 introduced chickens The breeds (recessive white-feathered broiler chicken and Anka red chicken) were obtained from the national-level local chicken breed gene bank of the Jiangsu Institute of Poultry Science. Each group selected 30 individuals (10 males and 20 females) according to the family line, and there was no difference between individuals. kinship. The sample is 1mL-1.5mL of wing vein blood, the anticoagulant is sodium citrate (ACD), and the sample is stored at -20°C for later...

Embodiment 2

[0065] Example 2 Application of Molecular Markers in Dongxiang Green-shell Layers

[0066] (a) Individual blood collection of Dongxiang green-shell layer hens: 0.5-1.0ml of venous whole blood from male and female hens randomly selected from the protected population (no less than 10 roosters and no less than 20 hens) with medical disposable syringes only), inject 2μL 0.5mol / L Na immediately after collection 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.

[0067] (b) DNA extraction and quality inspection: suck out 0.2-0.3ml of male and hen individual blood preserved in enzyme-free tubes at room temperature, and adopt the conventional animal peripheral blood phenol-phenol extraction method to extract individual blood DNA; agarose gel The DNA integrity was analyzed by electrophoresis, and the DNA purity was detected by a spectrophotometer; qualified samples were stored at -80°C for SNP detection.

[0068] (c) SNP marker de...

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Abstract

Relating to the field of genetics, the invention specifically provides a screening method and application of a Dongxiang blue-eggshell chicken genome SNP molecular marker. The screening method includes: conducting high-throughput sequencing detection, then calculating the genetic statistics, then analyzing the genetic difference between Dongxiang blue-eggshell chickens and other chicken breed groups, and finally screening out the Dongxiang blue-eggshell chicken SNP molecular marker. The screening method has the technical effects of efficient and rapid screening of Dongxiang blue-eggshell chicken molecular marker, the selected gene of the screened Dongxiang blue-eggshell chicken serves as the molecular marker for identifying the Dongxiang blue-eggshell chicken germplasm, has the characteristics of low cost, abundant data and high detection precision, alleviates the technical problems of high genetic differentiation research cost and low accuracy caused by lack of efficient molecular biological method and genetic marker in current Dongxiang blue-eggshell chicken genetic differentiation identification. And application of the Dongxiang blue-eggshell chicken genome SNP molecular markeralleviates the technical problem of lack of efficient molecular biological marker in the genetic differentiation research of Dongxiang blue-eggshell chicken.

Description

technical field [0001] The invention relates to the field of genetics, in particular to a screening method for genome SNP molecular markers of Dongxiang green-shell layer hens and an application thereof. Background technique [0002] Dongxiang Blue-Eggshell Chicken, an egg-type breed, was included in the "Jiangxi Livestock and Poultry Breeds" in 2001, included in the list of China's livestock and poultry genetic resources in 2004, and listed in the national level in 2014 List of livestock genetic resources. Dongxiang green-shell hens have a long history of breeding. During the long-term selection and domestication process, they have formed eggshell color characteristics that are completely different from other local chicken breeds. The eggs are of good quality and have strong stress resistance. It is a precious genetic resource, and it is also a well-developed and utilized local chicken breed resource in the market. [0003] Single nucleotide polymorphism (single nucleotid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6869
CPCC12Q1/6869C12Q1/6888C12Q2600/124C12Q2600/156C12Q2535/122
Inventor 韩威李国辉殷建玫张会永苏一军朱云芬朱静盛中伟沈海玉
Owner JIANGSU INST OF POULTRY SCI
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