Screening method and application of Dongxiang blue-eggshell chicken genome SNP molecular marker
A screening method, the technology of green-shell layer hens, applied in the field of genetics, can solve the problems of poor accuracy, limited amount of genetic variation information in the genome, and restrictions on the development and utilization of Dongxiang green-shell layer hen breed resources, so as to facilitate development, utilization, and detection The effect of high precision and rich data volume
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Embodiment 1
[0048] Example 1 Screening of Genomic SNP Molecular Markers of Dongxiang Green-shell Hens
[0049] (a) Blood sample collection: 18 local chicken breeds (Langshan Chicken, Anyiwa Gray Chicken, Gushi Chicken, Xianju Chicken, White-eared Yellow Chicken, Henan Gamecock, Jinhu Wufeng Chicken, Wenchang Chicken, Daweishan Micro Chicken, Tibetan Chicken, Camellia Chicken, Piao Chicken, Huiyang Bearded Chicken, Big Bone Chicken, Xiaoshan Chicken, Luyuan Chicken, Bian Chicken and Beijing You Chicken) and 2 introduced chickens The breeds (recessive white-feathered broiler chicken and Anka red chicken) were obtained from the national-level local chicken breed gene bank of the Jiangsu Institute of Poultry Science. Each group selected 30 individuals (10 males and 20 females) according to the family line, and there was no difference between individuals. kinship. The sample is 1mL-1.5mL of wing vein blood, the anticoagulant is sodium citrate (ACD), and the sample is stored at -20°C for later...
Embodiment 2
[0065] Example 2 Application of Molecular Markers in Dongxiang Green-shell Layers
[0066] (a) Individual blood collection of Dongxiang green-shell layer hens: 0.5-1.0ml of venous whole blood from male and female hens randomly selected from the protected population (no less than 10 roosters and no less than 20 hens) with medical disposable syringes only), inject 2μL 0.5mol / L Na immediately after collection 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.
[0067] (b) DNA extraction and quality inspection: suck out 0.2-0.3ml of male and hen individual blood preserved in enzyme-free tubes at room temperature, and adopt the conventional animal peripheral blood phenol-phenol extraction method to extract individual blood DNA; agarose gel The DNA integrity was analyzed by electrophoresis, and the DNA purity was detected by a spectrophotometer; qualified samples were stored at -80°C for SNP detection.
[0068] (c) SNP marker de...
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