Screening method and application of Henan gamecock genome SNP molecular markers
A molecular marker and genome technology, applied in the field of genetics, can solve the problems of low accuracy and high cost of Henan gamecock genetic differentiation research, and achieve the effect of low cost, abundant data and high detection accuracy
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Embodiment 1
[0062] Example 1 Screening of Henan gamecock genome SNP molecular markers
[0063] (a) Blood sample collection: Henan gamecock and 18 local chicken breeds of the control group (Langshan chicken, Anyiwa gray chicken, Gushi chicken, Xianju chicken, white-eared yellow chicken, Dongxiang green-shell chicken, Jinhu Wu Feng chicken, Wenchang chicken, Daweishan micro chicken, Tibetan chicken, camellia chicken, ladle chicken, Huiyang bearded chicken, big bone chicken, Xiaoshan chicken, Luyuan chicken, Bian chicken and Beijing oil chicken) and 2 introduced chicken breeds (hidden White-feathered broiler chicken and Anka red chicken) come from the national-level local chicken breed gene bank of Jiangsu Institute of Poultry Science. Each group selects 30 individuals (10 males and 20 females) according to the family line, and there is no relationship between individuals. 1 mL-1.5 mL of blood was collected from the wing vein, anticoagulated with sodium citrate (ACD), and stored at -20°C for...
Embodiment 2
[0079] Example 2 Application of Molecular Markers in Gamecocks in Henan
[0080] (a) Individual blood collection of Henan cockfighting preservation population: collect 0.5-1.0ml of venous whole blood from male and female chicken wings of the preservation population (no less than 10 roosters and no less than 20 hens are randomly selected), and inject the blood containing 2μL 0.5mol / L Na 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.
[0081] (b) DNA extraction and quality inspection: suck out 0.2-0.3ml of male and hen individual blood stored in enzyme-free tubes at room temperature, and extract individual blood DNA by conventional animal peripheral blood phenol-phenol extraction; The DNA integrity was analyzed by agarose gel electrophoresis, and the DNA purity was detected by a spectrophotometer; the qualified samples were stored at -80°C for use in SNP detection.
[0082] (c) SNP marker detection of Henan gamecock bree...
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