The invention discloses a method for quickly extracting
fungal DNA for PCR amplification, which comprises the steps of: (1) culturing fungi to obtain fungal mycelia; (2) placing the fungal myceliua into a centrifugal tube filled with a lysate; (3) alternately freezing and thawing the centrifugal tube; and (4) centrifuging the thawed fungal mycelia, taking a supernatant, adding 95 percent
ethanol into the supernatant, mixing the mixture evenly, quickly freezing the mixture by
liquid nitrogen, centrifuging the mixture, removing a supernatant, and air-
drying a precipitate to obtain the
fungal DNA. The method has simple operation, is time-saving and labor-saving, reduces the loss of the
fungal DNA during the
grinding, does not need
phenol and
chloroform for extraction, and avoids the contact of a toxic
reagent. The
DNA extracted by the method has good integrity, high purity and high yield, can be directly used for a PCR amplification reaction, and can efficiently amplify a target product for
cloning and sequencing. The method improves the extraction efficiency of the fungal
DNA, reduces the cost for the extraction of the fungal
DNA, and can effectively improve the
molecular identification and detection efficiency of the fungi.