The invention discloses a method for quickly extracting fungal DNA for PCR amplification, which comprises the steps of: (1) culturing fungi to obtain fungal mycelia; (2) placing the fungal myceliua into a centrifugal tube filled with a lysate; (3) alternately freezing and thawing the centrifugal tube; and (4) centrifuging the thawed fungal mycelia, taking a supernatant, adding 95 percent ethanol into the supernatant, mixing the mixture evenly, quickly freezing the mixture by liquid nitrogen, centrifuging the mixture, removing a supernatant, and air-drying a precipitate to obtain the fungal DNA. The method has simple operation, is time-saving and labor-saving, reduces the loss of the fungal DNA during the grinding, does not need phenol and chloroform for extraction, and avoids the contact of a toxic reagent. The DNA extracted by the method has good integrity, high purity and high yield, can be directly used for a PCR amplification reaction, and can efficiently amplify a target product for cloning and sequencing. The method improves the extraction efficiency of the fungal DNA, reduces the cost for the extraction of the fungal DNA, and can effectively improve the molecular identification and detection efficiency of the fungi.