Traditional Chinese medicine bulbus fritillariae cirrhosae DNA extraction reagent and extraction method
An extraction method and fritillary technology, which are applied in the field of traditional Chinese medicine fritillary DNA extraction reagents and extraction, can solve problems such as poor DNA integrity, and achieve the effects of optimizing treatment reagents, reducing pollution, and achieving good removal effects.
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Embodiment 1
[0036]A traditional Chinese medicine Fritillaria DNA extraction reagent, including nuclear lysate, CTAB buffer, TE Buffer, wherein:
[0037] Nuclear lysate includes: 1mol / L pH 8.0 Tris-HCl 100mL, 0.5mol / L pH 8.0EDTA 10mL, NaCl 14.6g, PVP-40 20g, β-mercaptoethanol 2ml, ddH 2 O was fixed to 1000mL;
[0038] CTAB buffer solution includes: CTAB 30g, NaCl 81.9g, 0.5mol / L pH 8.0EDTA 40mL, 1mol / L pH8.0 Tris-HCl 100mL, PVP-40 20g, β-mercaptoethanol 2ml, ddH 2 O was fixed to 1000mL;
[0039] TE Buffer includes: 1M pH8.0 Tris-HCl Buffer 10mL, 0.5M pH8.0 EDTA 2mL, ddH 2 O was adjusted to 1000mL.
[0040] The Chinese medicine Fritillaria DNA of the present embodiment is extracted by the following method:
[0041] (1) Take 100 mg of the ground sample, add 800 μL of nuclear lysate to each sample tube, vigorously shake and mix for 5 minutes, centrifuge at 12000 r / min for 3 minutes, and discard the supernatant;
[0042] (2) Repeat step (1) 1 to 2 times until the supernatant is not viscou...
Embodiment 2
[0053] A traditional Chinese medicine Fritillaria DNA extraction reagent, including nuclear lysate, CTAB buffer, TE Buffer, wherein:
[0054] Nuclear lysate includes: 1mol / L pH 8.0 Tris-HCl 90mL, 0.5mol / L pH 8.0EDTA 8mL, NaCl 13g, PVP-40 18g, β-mercaptoethanol 1.5ml, ddH 2 O was fixed to 1000mL;
[0055] CTAB buffer solution includes: CTAB 28g, NaCl 75g, 0.5mol / L pH 8.0EDTA 35mL, 1mol / L pH8.0 Tris-HCl 95mL, PVP-40 18g, β-mercaptoethanol 1.5ml, ddH 2 O was fixed to 1000mL;
[0056] TE Buffer includes: 1M pH8.0 Tris-HCl Buffer 10mL, 0.5M pH8.0 EDTA 2mL, ddH 2 O was adjusted to 1000mL.
[0057] The DNA extraction method of the traditional Chinese medicine Fritillaria in this example is the same as that in Example 1, and will not be described in detail here.
Embodiment 3
[0059] A traditional Chinese medicine Fritillaria DNA extraction reagent, including nuclear lysate, CTAB buffer, TE Buffer, wherein:
[0060] Nuclear lysate includes: 1mol / L pH 8.0 Tris-HCl 110mL, 0.5mol / L pH 8.0EDTA 12mL, NaCl 16g, PVP-40 22g, β-mercaptoethanol 2.5ml, ddH 2 O was fixed to 1000mL;
[0061] CTAB buffer solution includes: CTAB 32g, NaCl 85g, 0.5mol / L pH 8.0EDTA 45mL, 1mol / L pH8.0 Tris-HCl 105mL, PVP-40 22g, β-mercaptoethanol 2.5ml, ddH 2 O was fixed to 1000mL;
[0062] TE Buffer includes: 1M pH8.0 Tris-HCl Buffer 10mL, 0.5M pH8.0 EDTA 2mL, ddH2O to 1000mL.
[0063] The DNA extraction method of the traditional Chinese medicine Fritillaria in this example is the same as that in Example 1, and will not be described in detail here.
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