A micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen

An unprocessed, semen technology, used in tissue cell/virus culture devices, biomass post-processing, laboratory containers, etc.

Active Publication Date: 2018-09-04
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This begs the question of whether ICSI bypasses important and corresponding natural selection barriers for spermatozoa
[0004] Currently, there are no reliable methods to non-invasively assess sperm properties that may be specifically related to successful conception and delivery when using ICSI

Method used

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  • A micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen
  • A micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen
  • A micro-fluidic device for selective sorting of highly motile and morphologically normal sperm from unprocessed semen

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Embodiment Construction

[0047] For the purposes of the present invention, the journey of sperm through the maternal reproductive tract - a phenomenon preserved in viviparous mammals over millions of years of evolution and reproduction - is considered to be a naturally available function for fertile sperm "filter". Simulate the physical components of the pathway in vitro using a microfluidic approach, design through multiscale computer simulations incorporating fluid physics, and examine how this artificial cervix-uterus-fallopian tube pathway affects the descriptive characteristics of spermatozoa, and finer fertile spermatozoa Clinically relevant measurements including DNA integrity and methylation status.

[0048] The developed SPARTAN (Simple Periodic Array for Trapping and Isolation) method successfully mimics the basic filtering features of the natural pathway and isolates highly motile and morphologically normal spermatozoa with high DNA integrity and low epigenetic variation, For use in fertil...

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Abstract

A microfluidic chip is provided for self-sorting highly motile, morphologically normal sperm cell with high DNA integrity from a fresh semen sample. The sperm self-sorting microfluidic chip has one ormore inlet chambers, and sperm collection outlet chamber(s), and the middle of the channel features various micro-fabricated structures in different geometrical shapes and orientations, with varyingperiodicities and patterns, such as an array of micro-fabricated pillars that facilitate the transport of the active and healthy sperm into the outlet chamber.

Description

field of invention [0001] The present invention relates to a method, device and system for sorting highly motile, morphologically normal and / or genetically normal sperm from unprocessed semen. Background of the invention [0002] Human infertility is a worldwide disease, affecting an estimated 80 million couples each year. In developed countries, 1–3 percent of newborns are conceived through assisted reproduction, most commonly through in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) [1]. One-third of the causes of infertility are male, and many of these conditions are treated with assisted reproductive technology (ART) [2,3]. While potent enough to bypass human infertility, assisted reproductive technologies typically do not, and the live birth rate per cycle averaged less than 35% in the United States in 2014 [4]. In addition, ART may have associated risks for offspring including: (a) increased risk of sex chromosome abnormalities, (b) increased r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M3/06C12N5/071
CPCC12N5/0612C12N5/061C12M47/04C12M23/16B01L3/502761B01L3/502746B01L2300/0832B01L2200/0652B01L2200/027
Inventor U·德米尔奇E·图泽尔J·L·金斯利T·琴那萨米
Owner THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
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