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Product for detecting sperm quality and use method thereof

A sperm quality and product technology, applied in the field of chemistry, can solve problems such as difficulties in accurate and comprehensive diagnosis, and achieve the effects of short cycle, accurate test results and simple steps

Active Publication Date: 2021-03-09
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this detection method requires the tested male to have enough sperm, and if the tested person is a patient with oligospermia, it is difficult to make an accurate and comprehensive diagnosis

Method used

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  • Product for detecting sperm quality and use method thereof
  • Product for detecting sperm quality and use method thereof
  • Product for detecting sperm quality and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0034] Experimental example 1: Correlation experiment between different piRNAs and male sperm quality

[0035] 1.1 Experimental materials

[0036] 1.1.1 Main reagents and consumables

[0037] 1) Xylene, paraformaldehyde, potassium chloride, potassium dihydrogen phosphate, sodium chloride, disodium hydrogen phosphate absolute ethanol: Sinopharm Chemical Reagent Co., Ltd.;

[0038] 2) Sperm staining solution kit: Nanjing Xindi Biopharmaceutical Co., Ltd.;

[0039] 3) piRNAs extraction kit: Beijing Aidlab Co., Ltd.;

[0040] 4) Reverse transcription kit: Beijing Aidlab Co., Ltd.;

[0041] 5) Fluorescent quantitative PCR kit: Beijing Aidlab Co., Ltd.;

[0042] 1.1.2 Main instruments and equipment

[0043] 1) Pressure steam sterilizer: Shanghai Shen'an Medical Instrument Factory;

[0044] 2) Electronic balance, PH meter: Germany sartorius company;

[0045] 3) Magnetic stirrer: Shanghai Sile Instrument Co., Ltd.;

[0046] 4) Pure water meter: American Millipore Company;

[...

experiment example 2

[0143] Experimental example 2: A product for testing sperm quality

[0144] This embodiment provides a product for detecting sperm quality, comprising hsa_piR_016118, specific primers for amplifying hsa_piR_016118 and / or reverse transcription primers for reverse transcription of hsa_piR_016118 into cDNA;

[0145] comprising hsa_piR_017507, specific primers for amplifying hsa_piR_017507 and / or reverse transcription primers for reverse transcription of hsa_piR_017507 into cDNA;

[0146] And / or, comprising hsa_piR_006215, specific primers for amplifying hsa_piR_006215 and / or reverse transcription primers for reverse transcription of hsa_piR_006215 into cDNA;

[0147] Wherein, the nucleotide sequence of hsa_piR_016118 is shown in SEQ ID NO:1; the nucleotide sequence of hsa_piR_017507 is shown in SEQ ID NO:2; the nucleotide sequence of hsa_piR_006215 is shown in SEQ ID NO:3; for amplification The nucleotide sequence of the specific primer for amplifying hsa_piR_016118 is shown in ...

experiment example 3

[0148] Experimental Example 3: A method of using a product for testing sperm quality

[0149] This embodiment provides the use method of the product for detecting sperm quality in embodiment 2, the use method is:

[0150] Take the seminal plasma, first use the miRNA extraction kit to extract hsa_piR_016118 in the seminal plasma, then use the reverse transcription primers used to reverse transcribe hsa_piR_016118 into cDNA to reverse transcribe the extracted hsa_piR_016118 into cDNA, and then according to the cDNA obtained by reverse transcription, by RT-qPCR detected the expression of hsa_piR_016118 in seminal plasma relative to the internal reference U6 (2 Ct,U6-Ct,016118 ), and finally judge the sperm quality according to the expression of hsa_piR_016118 relative to the internal reference U6. The seminal plasma with the expression of hsa_piR_016118 less than 0.86529154 corresponds to the sperm of men with non-asthenozoospermia and non-teratozoospermia, and the sperm with the...

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Abstract

The invention relates to a product for detecting the sperm quality and a use method thereof, and belongs to the technical field of medicines. The invention provides the product for detecting the spermquality; the product takes hsa_piR_016118, hsa_piR_017507 and / or hsa_piR_006215 as piRNA markers related to the sperm quality, wherein the expression quantity of the hsa_piR_016118 has strong correlation with the normal sperm motility; the expression quantity of the hsa_piR_017507 and the expression quantity of the hsa_piR_006215 have strong correlation with the normal sperm morphology; furthermore, all mature males have seminal plasma; even a male with oligospermia also has enough seminal plasma; and therefore, the product can be used for accurately detecting male patients with enough spermsand oligospermia patients, and is wide in application range.

Description

technical field [0001] The invention relates to a product for detecting the quality of sperm and its application method, belonging to the field of chemical technology. Background technique [0002] The survey found that the average infertility rate in my country now exceeds 15%. There are about 20 million infertile couples in the country, and the infertility caused by the man's reproductive health accounts for about 30%. Compared with the past, the proportion of men The proportion of male infertility is increasing, and the proportion of male infertility is becoming more and more important in social problems. However, after the full implementation of my country's family planning policy, people's requirements for prenatal and postnatal care are getting higher and higher. Therefore, it is very important to accurately detect the quality of male sperm to ensure the quality of fertility. [0003] The quality of male sperm mainly includes two aspects: motility and morphology. At ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6888C12Q1/6851C12N15/11
CPCC12Q1/6851C12Q1/6883C12Q1/6888C12Q2600/158C12Q2600/178C12Q2521/107C12Q2525/173C12Q2531/113
Inventor 薛波新刘晓龙
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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