A quick method for obtaining triploids of Brevis
A Dengzhanhua and triploid technology, which is applied in the field of plant genetics and breeding, can solve the problems of inability to meet the needs of breeding and utilization, low occurrence frequency, etc.
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Embodiment 1
[0019] Select 1,000 plump seeds of Erigeron breviscapus harvested in the same year.
[0020] After adding an appropriate amount of distilled water for pre-culture for 16 hours, soak in 50 mL of Oryzalin aqueous solution with a concentration of 60 μmol / L for 24 hours. After the treatment, wash with tap water 3 times.
[0021] Mix the treated seeds with river sand, spread them, cover them with fine soil with a thickness of about 2 mm, spray water to moisten them, and then carry out normal seedling management.
[0022] When the seed emerges with 4-6 true leaves, tear off the lower epidermis of the fourth true leaf for stomatal identification. The specific method is as follows: measure the length and width of the stomata under a microscope, and calculate the perimeter of the stomata according to the ellipse perimeter formula, and measure 30-50 stomata for each plant. Statistical T-test method was used to determine whether the stomatal perimeter of the plant was different from...
Embodiment 2
[0027] Select 1,000 plump seeds of Erigeron breviscapus harvested in the same year.
[0028] After adding an appropriate amount of distilled water for pre-culture for 16 hours, soak in 50 mL of Oryzalin (Oryzalin) aqueous solution with a concentration of 90 μmol / L for 72 hours. After the treatment, wash with tap water 3 times.
[0029] Mix the seeds with river sand, spread them, cover them with fine soil about 2mm thick, spray them with water to moisten them, and then carry out normal seedling management.
[0030] When the seed emerges with 4-6 true leaves, tear off the lower epidermis of the fourth true leaf for stomatal identification. The specific method is as follows: measure the length and width of the stomata under a microscope, and calculate the perimeter of the stomata according to the ellipse perimeter formula, and measure 30-50 stomata for each plant. Statistical T-test method was used to determine whether the stomatal perimeter of the plant was different from t...
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