3 results about "Plant chromosomes" patented technology

The invention discloses an identification method of a sequence participating in potato centromere relocation, and belongs to the technical field of plant heredity and molecular biology. According to the method, T2T-level genome data and CENH3 ChIP-seq data of different haplotypes of potatoes are integrated, sequence screening, annotation and function prediction are carried out by utilizing a bioinformatics analysis tool, and a satellite DNA sequence which is 2-3Kbp in length, is highly conserved in the same haplotype and can be specifically combined with CENH3 protein is identified. The sequence can induce CENH3 to deposit in a non-native centromere area, so that functional relocation of centromere is realized, and a core molecular element and a technical support are provided for artificial centromere construction, plant chromosome engineering and accurate genome operation. The method solves the problems that in the prior art, a functional sequence for clearly inducing centromere relocation is lacked, a CENH3 targeted recruitment mechanism model is not established and the like, and has a wide application prospect.

This invention belongs to the field of plant sex identification technology, and particularly relates to the application of a molecular marker in identifying the sex of *Zephyranthes* plants. The molecular marker is linked to the Y chromosome of *Zephyranthes* plants and comprises a sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity with SEQ ID NO:1. The molecular marker provided by this invention can be tightly linked to the Y chromosome of *Zephyranthes* plants, thereby achieving the purpose of early and rapid sex detection. This has significant practical implications for sex selection and proportioning of ornamental garden plants, understanding and protecting endangered *Zephyranthes* germplasm resources, and improving the effectiveness of ex-situ and in-situ conservation management.

The present application relates to the technical field of plant chromosome preparation, and specifically discloses a method for increasing the number of mitotic metaphase chromosomes of sugarcane or sugarcane relative genus root tips, comprising: soaking sugarcane or sugarcane relative genus stem double bud segments in an indole-3-acetic acid solution with a concentration of 60-120 mg / L, and culturing in sand or peat substrate; root tip pretreatment: culturing for 2-3 days, selecting healthy and tender root tips, and placing the root tips in saturated p-dichlorobenzene aqueous solution for low-temperature treatment at 8-12 DEG C for 3-5 h; using Carnoy's fixative for fixation; enzymolysis of the root tip growth point; smearing the root tip on a glass slide for preparation; and staining treatment. The method for preparing the present application can achieve the fastest time of only 4 days from root tip culture to chromosome observation, has a short cycle, high efficiency, is convenient and fast for sampling, has a large number of metaphase division phases in the chromosomes, clear morphology, a clean glass slide background, and is convenient for chromosome counting observation and karyotype analysis, and has important significance for the development of sugarcane cytogenetics research.