Lagerstroemia plant stem tip chromosome tablet preparation method

A technology of crape myrtle and shoot apex, which is applied in the field of crape myrtle shoot apex chromosome production, can solve problems such as inconsistent reporting of plant chromosome numbers, limitations, and difficulty in identifying chromosomes, so as to expand the range of materials, improve production efficiency, and avoid poisoning. effect of action

A technology of crape myrtle and shoot apex, which is applied in the field of crape myrtle shoot apex chromosome production, can solve problems such as inconsistent reporting of plant chromosome numbers, limitations, and difficulty in identifying chromosomes, so as to expand the range of materials, improve production efficiency, and avoid poisoning. effect of action

CN102645360AActive Publication Date: 2012-08-22BEIJING FORESTRY UNIVERSITY
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  • Lagerstroemia plant stem tip chromosome tablet preparation method
  • Lagerstroemia plant stem tip chromosome tablet preparation method
  • Lagerstroemia plant stem tip chromosome tablet preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Lagerstroemia genus Yakushima shoot tip chromosome preparation method of Lagerstroemia genus of the present invention

[0039] Lagerstroemia genus Yakushima Lagerstroemia [Yakushima Lagerstroemia is planted in the National Flower Center (Xiaotangshan, Beijing) Germplasm Resource Garden]. At 9:00 in the morning, the shoot tip part of the innermost growth point of the shoot tip of the annual plant with a length of 0.5 cm was taken. Put them directly into the fixative solution (by volume, absolute ethanol: glacial acetic acid (analytical grade): chloroform (analytical grade) = 5:3:2) and fix in a -20°C refrigerator for 18 hours. Take it out before the experiment, rinse and wash the fixative in distilled water, and transfer it to 0.075mol / L KCl solution before hypotonicity for 30 minutes; rinse the growth point of the shoot tip after the previous hypotonic treatment with distilled water, dry it with absorbent paper, and put it in Enzymolysis was performed in a ...

Embodiment 2

[0041] Embodiment 2 Lagerstroemia genus Fujian Lagerstroemia shoot tip chromosome preparation method of the present invention

[0042] Lagerstroemia genus Fujian Ziwei [Fujian Lagerstroemia is planted in the National Flower Center (Xiaotangshan, Beijing) Germplasm Resource Garden], at 11:00 in the morning, the length of the innermost growth point of the new stem tip growth point after taking the branches trimmed and refrigerated in early winter after hydroponics is 1.0cm of the tip of the stem. Put them directly into the fixative solution [according to volume ratio, absolute ethanol: glacial acetic acid (analytical grade): chloroform (analytical grade) = 5:3:2] and fix in a -20°C refrigerator for 24 hours. Take it out before the experiment, rinse and wash the fixative in distilled water, and transfer it to 0.075mol / L KCl solution before hypotonicity for 20 minutes; rinse the growth point of the shoot tip after the previous hypotonic treatment with distilled water, dry it with ...

Embodiment 3

[0044] Example 3 Conventional Tablet Method Lagerstroemia genus Yakushima Lagerstroemia Chromosome Preparation Method

[0045] Tablets are made by conventional tableting method. Lagerstroemia genus Yakushima crape myrtle, the root tip of the seed germination was taken at 9:00 in the morning. Put into 0.002mol / L 8-hydroxyquinoline for pretreatment at room temperature at 20-25°C for 3h. Then the root tips were washed with distilled water, transferred to Carnoy's fixative solution (absolute ethanol: glacial acetic acid = 3:1) and stored overnight at 4°C. Take it out before the experiment, rinse and wash the fixative in distilled water, and transfer it to 0.075mol / L KCl solution before hypotonicity for 30 minutes; rinse the root tip after the pre-hypotonic treatment with distilled water, dry it with absorbent paper, and put it in concentrated hydrochloric acid Dissociate with ethanol mixture (1:1) at room temperature for 1h 20min; after dissociation, rinse with distilled water a...

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Abstract

The invention discloses a lagerstroemia plant stem tip chromosome tablet preparation method which comprises the following steps of: selecting a part with a length of 0.5-1.0 cm at the innermost side of a lagerstroemia plant annual branch stem tip growing point or a newly-grown stem tip growing point after a branch which is pruned or refrigerated in early winter is subjected to water culture, fixing with stationary liquid (Ethanol: glacial acetic acid: chloroform is equal to 5:3:2 according to the to volume ratio), carrying out hypotonic treatment, carrying out hypotonic treatment again after mixed enzyme liquid is subjected to enzymatic hydrolysis and preparing a tablet by adopting a flame drying method. The chromosome tablet preparation method has the beneficial effects that the processes of pretreatment, dissociation and chromosome tabletting are omitted, the tablet preparation efficiency is improved through directly fixing, the toxic effect of pretreatment liquid and dissociation liquid on a chromosome is avoided, the original form of the chromosome is maintained, the better chromosome dispersion effect is obtained, and meanwhile, the sampling range and the time of lagerstroemia plant stem tip chromosome preparation are enlarged. The tablet prepared by the method can be used for a fluorescence in-situ hybridization experiment to lay a good foundation for the further research of a lagerstroemia genome.

Description

technical field [0001] The invention relates to a method for preparing chromosomes of plants, in particular to a method for preparing chromosomes of shoot tips of the genus Lagerstroemia. Background technique [0002] Lagerstroemia belongs to Lythraceae, and its main species, such as Lagerstroemia grandiflorum and Lagerstroemia urophylla, are important ornamental flowers and trees in summer in my country. Compared with other garden flowers, the research on the chromosomes of Lagerstroemia genus lags far behind, and most of the plants of this genus have small and medium chromosomes, which are not easy to dye. It is difficult to identify their chromosomes by classical cytological methods, which limits the in-depth understanding of the molecular cytogenetic mechanism of Lagerstroemia genus. carried out. [0003] Because most plants of the genus Lagerstroemia are aneuploid and have small chromosomes (Graham and Cavalcanti, 2001), the number of chromosomes reported in this genus...

Claims

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Application Information

Patent Timeline
22 Aug 2012
Publication
CN102645360A
IPC
G01N1/28
Inventors
潘会堂; 杨冰洁