28 results about "Lagerstroemia indica" patented technology

The application discloses a method for inducing dedifferentiation of anther of Lagerstroemia indica to form a callus. The application selects the anther of Lagerstroemia indica as an explant for dedifferentiation induction, and through a large number of experiments, the most suitable dedifferentiation culture medium formula and culture conditions are screened out, thereby providing reference value for future development of related tests. The explant inoculation contamination rate is less than 10%, the average dedifferentiation culture rate can reach 35%, and the callus cell mass develops healthily. The dedifferentiation induction efficiency of the woody Lagerstroemia indica anther is improved.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a primer for identifying the authenticity of a crape myrtle hybrid as well as application and a method thereof. The primer molecular marker is high in resolution, can be used independently or in combination, and can be used for quickly and accurately identifying the authenticity of the crape myrtle hybrid and identifying the crape myrtle variety in the seedling stage. When the primer is used for identifying the authenticity of the lagerstroemia indica hybrid, the identification rate of hybrid offspring can reach 90%, and the primer is suitable for popularization and application in actual use. The method is simple to operate, convenient and fast, can directly utilize existing equipment, reagents and the like, and is convenient to popularize and use.

The application discloses a method for tissue culture and rapid propagation of bigflower Lagerstroemia indica, and comprises the following steps: (1) selecting tender branches of bigflower Lagerstroemia indica; (2) disinfecting bigflower Lagerstroemia indica explants; (3) inducing and proliferating adventitious buds of bigflower Lagerstroemia indica; (4) rooting culture of bigflower Lagerstroemia indica; (5) acclimatization of bigflower Lagerstroemia indica tissue culture seedlings; and (6) transplanting bigflower Lagerstroemia indica. The method can realize rapid propagation of bigflower Lagerstroemia indica by optimizing the tissue culture and rapid propagation process of bigflower Lagerstroemia indica, and has high technical content, significant technical advantages and wide application prospect, solves problems, such as long cycle, low efficiency and genetic instability, in traditional propagation methods, and injects strong impetus into transformation and upgrading and high-quality development of the flower industry, and greatly meets the development demand of the tissue culture and rapid propagation industry of bigflower Lagerstroemia indica.

The invention relates to a method for overcoming distant hybridization obstacle of lagerstroemia indica and lythrum salicaria by gibberellin, which comprises the following steps: castration: when lagerstroemia indica flowers do not completely bloom and loosen pollen, removing stamens on the flowers with tweezers, and sleeving the flowers with sulfuric acid paper bags for isolation; spraying a gibberellin solution, namely uniformly spraying the gibberellin solution on pistil stigmas of the crape myrtle; pollination: dipping pollen of lythrum salicaria in a cotton swab in sunny weather without strong wind, smearing the pollen on pistil stigmas of lagerstroemia indica treated by a gibberellin solution, bagging in time after pollination, and marking. The technical problem to be solved by the invention is to promote distant hybridization, fertilization and fruit setting of crape myrtle and lythrum salicaria by spraying gibberellin to stigmas before pollination, so that distant hybridization obstacles are overcome.

The application provides a pollination box and a method for using the same in pollination of Lagerstroemia indica. The pollination box comprises a box body, a wind supply mechanism and a plurality of heat preservation units. The heat preservation unit comprises a base, a loading cup and a valve mechanism. The valve mechanism is used for synchronously blocking or opening the connection state of the air inlet hole and the first air inlet channel and the connection state of the air outlet hole and the first air outlet channel. The wind supply mechanism is used for delivering cold air to the first air inlet channel and for discharging the airflow in the first air outlet channel out of the box body. When the loading cup is installed in the installation groove, the loading cup covers the slot of the installation groove. The loading cup drives the valve mechanism to open the air inlet hole and the air outlet hole, so as to make the air inlet hole communicate with the first air inlet channel and make the air outlet hole communicate with the first air outlet channel. When the loading cup is taken out of the installation groove, the valve mechanism closes the air inlet hole and the air outlet hole. The pollination box can maintain the temperature of the stamen in the loading cup in a certain range, so as to ensure the viability of the pollen and improve the pollination success rate and work efficiency.

The application provides a traditional Chinese medicine preparation for treating diabetic nephropathy and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine pharmacy. The traditional Chinese medicine preparation is prepared from traditional Chinese medicinal materials such as angelica, eucommia ulmoides, helicteres angustifolia, broussonetia papyrifera, radix paeoniae suffruticosae, curcuma longa, dendrobium, polygonatum, radix adnati, ligustrum lucidum, ipomoea purpurea, lagerstroemia indica, kochia scoparia, haes, pyrrosia lingua and herba barbatae, and has the effects of nourishing yin and kidney, promoting blood circulation to remove meridian obstruction and clearing heat and removing dampness. The traditional Chinese medicine preparation can up-regulate the expression levels of podocin and nephrin, inhibit inflammatory mediators, improve the blood sugar, urine protein, kidney function and kidney pathological injury of DN rats, provides a new idea for the treatment of diabetic nephropathy, and has a good development and application prospect.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Bv47 in crop growth promotion and verticillium dahliae antagonism. The strain can secrete indoleacetic acid, cellulase, protease and amylase, has various growth promoting functions of dissolving potassium, producing ammonia and the like, has good high temperature resistance and ultraviolet light resistance, and has the preservation number of CGMCC No.36499. A plate confrontation test shows that the strain has a remarkable inhibition effect on various plant pathogenic bacteria including verticillium dahliae. A pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be obviously reduced by inoculating the strain, and meanwhile, the growth indexes such as the root length, the plant height, the stem diameter, the fresh weight, the leaf area and the root dry weight of cotton plants are obviously promoted. The strain provides an efficient and green microbial resource for biological control of cotton verticillium wilt and cotton growth promotion, and has a good agricultural application prospect.

The invention discloses a tissue-culture-free agrobacterium rhizogenes-mediated genetic transformation system method for crape myrtle hairy roots, and relates to the technical field of molecular biology.The method comprises the steps that an overexpression vector carrying an RUBY visual tag is constructed, agrobacterium rhizogenes K599 is introduced, and a positive engineering strain is obtained through resistance screening and molecular verification; propagating the positive engineering strain, centrifugally collecting thalli, resuspending by using a buffer solution containing acetosyringone, adjusting the concentration of a bacterial solution, and standing and activating to form a bacterial suspension for infection; the method comprises the following steps: selecting current-year semi-lignified branches of crape myrtle to prepare cutting slips, retaining two pairs of bud points, and performing incision treatment; the cutting slips are completely immersed in the bacterial suspension for infection, and agrobacterium tumefaciens-mediated genetic transformation is completed in combination with vacuum-assisted treatment; taking out the cutting slips, temporarily dipping the cutting slips in a rooting aqueous solution, and then cutting the cutting slips in a sterile substrate formed by mixing vermiculite and perlite; and culturing for 24 hours under a dark condition, and continuously culturing for 30 days in a high-temperature and high-humidity environment.

The present application relates to the technical field of crape myrtle germplasm identification, and particularly relates to a molecular marker related to purple leaf character of crape myrtle and application thereof. The molecular marker is designed based on 32 bp insertion / deletion at 789140-789171 bp of chromosome 12 of crape myrtle reference genome, and the polymorphism of bases at the site can be used for early identification of the purple leaf character of crape myrtle, especially early identification of the germplasm with perennial purple leaves, purple young leaves and green mature leaves. The InDel molecular marker and primer provided by the present application are high in accuracy, strong in specificity, good in stability, simple and convenient in detection, high in economy and efficiency, and can be widely applied to early accurate identification of leaf color in crape myrtle natural population and hybrid population, and provide technical support for molecular marker assisted genetic breeding of the purple leaf character of crape myrtle.

The invention belongs to the technical field of ornamental plant evaluation, and particularly discloses a lagerstroemia indica salt-tolerant germplasm screening method based on ornamental value and cultivation performance, which comprises the following steps: S1, hybridization: performing hybridization by taking a lagerstroemia indica variety of Yakuishan and a low lagerstroemia indica variety as parents; s2, sowing, seedling raising and transplanting; s3, determining the soil property of the saline-alkali soil; s4, determining the ornamental value of the lagerstroemia indica cultivated in the saline S5, observing the cultivation performance of the crape myrtle; s6, constructing a comprehensive evaluation system for ornamental characters and cultivation performance of the lagerstroemia indica in the saline-alkali soil area; and S7, evaluating salt-tolerant related phenotypes and physiological indexes of the I-grade lagerstroemia indica strain with ornamental value and cultivation performance. According to the lagerstroemia indica salt-tolerant germplasm screening method based on the ornamental value and the cultivation performance, comprehensive character scoring is conducted on different offspring of a lagerstroemia indica resistant group according to the hierarchical structure evaluation standard, the ornamental value, the salt-tolerant capacity and the cultivation performance of offspring individuals are identified, and salt-tolerant strains are obtained through screening.

The invention relates to the technical field of gene engineering application, and particularly discloses a key gene LiRabA4a for regulating and controlling the leaf color of crape myrtle and application thereof. The nucleotide sequence of the gene LiRabA4a is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a key gene LiRabA4a for regulating and controlling the leaf color of lagerstroemia indica. Through qRT-PCR analysis, it is found that the LiRabA4a gene is remarkably predominantly expressed in crape myrtle leaves, and the transcriptional level of the LiRabA4a gene is related to the leaf color yellowing degree; the LiRabA4a gene is overexpressed through an agrobacterium tumefaciens-mediated genetic transformation method, so that the chlorophyll content of the crape myrtle can be increased, and the leaf color is greener; liRabA4a gene expression is inhibited, so that the chlorophyll content is reduced, and the leaf color is yellow; the invention further relates to heterologous expression of the LiRabA4a gene in catalpa bungei for remarkably promoting growth and development of catalpa bungei. The LiRabA4a gene provided by the invention can be used for directional genetic improvement of the leaf color of the lagerstroemia indica, can be used for cultivating lagerstroemia indica varieties with different leaf colors, and has an important application value in the aspects of gene engineering and molecular design breeding of the lagerstroemia indica.

This invention discloses a method for preparing a crape myrtle fruit plant detox gel, specifically relating to the field of feminine hygiene formulation technology. Using crape myrtle fruit extract as the core active ingredient, combined with Bacillus fermentation products, the gel is prepared through a fully optimized process involving precise raw material selection, supercritical high-efficiency extraction, multi-stage deep purification, targeted fermentation for activation, and aseptic and gentle molding. The crape myrtle fruit extract undergoes a five-stage activation-locking process. This invention achieves dual high-efficiency retention of crape myrtle fruit active ingredients and increased purity, as well as improved Bacillus antimicrobial peptide yield, through a process of supercritical CO2 extraction, multi-stage membrane separation, low-temperature crystallization and segmented temperature-controlled targeted fermentation, and low-temperature freeze-drying. The product's pH value is precisely controlled to 3.8-4.5, exhibiting extremely high antibacterial rates against common pathogens such as Escherichia coli. This overcomes the pain points of existing products, such as low activity, poor stability, and difficulty in balancing efficacy and safety, achieving highly efficient synergistic care for postmenstrual cleansing, antibacterial action, balancing, and repair.

The application discloses a method for regulating flowering period of Lagerstroemia indica and application thereof, and comprises the following steps: S1, daily management of Lagerstroemia indica; S2, operation of regulating flowering period of Lagerstroemia indica; S3, maintenance of Lagerstroemia indica after regulating flowering period; wherein, the step S2 comprises: S21, selecting suitable plants, S22, pruning inflorescences or pruning branches of the plants in the middle and late May to the late August according to the target of regulating flowering period. The method for regulating flowering period of Lagerstroemia indica provided by the application regulates the secondary flowering period of Lagerstroemia indica to the period from September to the middle and early October, enriches the types of woody flower viewing plants in the period of major festivals (such as the National Day), reduces the amount of grass flowers, guarantees the flower landscape effect, and reduces the maintenance cost. The method for regulating flowering period of Lagerstroemia indica provided by the application is high in accuracy, strong in practicability and convenient to popularize.

The invention provides a crape myrtle seedling breeding fertilization optimization system and method. A first physiological parameter reflecting the growth rate of crape myrtle seedlings and a second physiological parameter reflecting the nutritional status of crape myrtle seedling leaves are fused into a nutrition stress index of crape myrtle seedling breeding; when the nutrition stress index is lower than a topdressing critical value of the lagerstroemia indica seedlings, triggering a stage topdressing instruction of a seedling topdressing device, and performing differential configuration on nutrition supply proportions in the current growth stage according to nutrition demand gravity centers of the lagerstroemia indica seedlings in different growth stages when topdressing is triggered, the mass concentration ratio of all nutrient elements in the seedling topdressing device is obtained; the topdressing nutrient solution of the seedling topdressing device is individually prepared according to various mass concentration ratios, a stage topdressing instruction of the crape myrtle seedlings is completed, then physiological status monitoring is continuously conducted on crape myrtle seedling breeding, and multi-stage topdressing in crape myrtle seedling breeding is completed. Based on the scheme, the multi-stage topdressing decision of crape myrtle seedling breeding can be realized.

The application provides application of a Lagerstroemia indica LiTCP14 gene in reducing leaf size of Lagerstroemia indica, and a coding sequence of the LiTCP14 gene is shown as SEQ ID NO:1. The application adopts a VIGS system to silence expression of the Lagerstroemia indica LiTCP14 gene, and a LiTCP14 gene silencing strain is obtained, and the LiTCP14 gene expression level and the leaf size of the LiTCP14 gene silencing strain are both significantly reduced compared with a wild type strain, which indicates that silencing expression of the Lagerstroemia indica LiTCP14 gene can obtain a small leaf Lagerstroemia indica, and has certain practical value in Lagerstroemia indica breeding.

The application discloses a kind of SSR marker primers for quickly identifying variety of Lagerstroemia indica, and application and method thereof.The SSR marker primer is one or more than two of primer S01, primer S11 and primer S21;10 kinds of Lagerstroemia indica varieties can be specifically identified, molecular marker resolution is high, and it can be used alone or in combination.The identification method is simple to operate, and is conducive to popularization and application.

The present invention relates to a method for overcoming obstacles to distant hybridization between crape myrtle and loosestrife using gibberellins. The method comprises the following steps: emasculation: before the crape myrtle flowers fully open and shed pollen, the stamens on the flowers are completely removed with tweezers and covered with a sulfuric acid paper bag for isolation; mist spraying a gibberellin solution: evenly spraying the gibberellin solution on the stigma of the crape myrtle pistil; and pollination: on sunny and windless days, using a cotton swab to dip loosestrife pollen, applying the pollen to the stigma of the crape myrtle pistil treated with the gibberellin solution, and promptly bagging and marking the flowers after pollination. The technical problem to be solved by the present invention is to promote fertilization and fruit setting in distant hybridization between crape myrtle and loosestrife by mist spraying the stigma with gibberellins before pollination, thereby overcoming the obstacles to distant hybridization.

1. Name of the designed product: decorative column ( Lagerstroemia indica flower). 2. Use of the designed product: the designed product is used for building decoration. 3. Design points of the designed product: in shape. 4. Picture or photo best indicating the design points: design 1 perspective view. 5. Design 1 is designated as the basic design. 6. Other circumstances needing explanation: design 2 claims color.

The application discloses LiMYB75L and / or LiEGL3 genes, proteins and application thereof in flower color regulation of Lagerstroemia indica. The application discloses R2R3 MYB (LiMYB75L) transcription factors, basic helix-loop-helix bHLH (LiEGL3) transcription and WDR (LiTTG1-2) proteins of Lagerstroemia indica and a promoter (proDFR) of a downstream gene LiDFR gene. Three genes LiMYB75L, LiEGL3, LiTTG1-2 and proDFR are cloned in two different materials, it is proved that down-regulation of LiMYB75L and LiEGL3 in vivo of Lagerstroemia indica can down-regulate anthocyanin content of petals, and expression of multiple genes in an anthocyanin synthesis pathway is also down-regulated. Meanwhile, it is proved that LiMYB75L and LiEGL3 are located in a cell nucleus and interact with LiTTG1-2 to form a ternary complex.

The invention provides a traditional Chinese medicine preparation for treating diabetic nephropathy and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine preparation. The traditional Chinese medicine preparation is prepared from the following traditional Chinese medicine materials: angelica sinensis, eucommia ulmoides, flatstem milkvetch seed, fructus broussonetiae, mantis egg-case, turmeric, dendrobe, radix polygonati officinalis, radix glehniae, glossy privet fruit, campsis grandiflora, crape myrtle flower, fructus kochiae, lygodium japonicum, pyrrosia lingua, sculellaria barbata and the like. The traditional Chinese medicine preparation has the effects of nourishing yin and tonifying kidney, promoting blood circulation to remove meridian obstruction, clearing heat and promoting diuresis. The traditional Chinese medicine preparation can achieve the effect of improving blood sugar, urine protein, renal functions and kidney pathological injuries of DN rats by up-regulating expression levels of podocin and nephrin and inhibiting inflammatory mediators, provides a new thought for treatment of diabetic nephropathy, and has good development and application prospects.

1. The name of the design product: teapot (lagerstroemia indica star). 2. The use of the design product: for containing liquid beverages. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: design 1 perspective view 1. 5. Design 1 is designated as the basic design.

The application discloses a kind of based on low-tide flat regional plant planting method, including tidying planting area, setting one or more rows of wood piles on the side close to seawater, and forming planting area between the outside of wood pile and the shore side, and laying a layer of backfill layer on the planting area;Base disc arrangement, a plurality of base discs are arranged in staggered intervals on the backfill layer, and the base disc is used for planting plants;The base disc includes frame body, groove body, planting soil, water collecting tank, drain pipe and gravel layer;Zoning planting, from the side close to wood pile to the shore side is sequentially divided into a zone, a second zone and a third zone, Aegiceras corniculatum is planted in the zone, Kandelia candel is planted in the second zone, and Lagerstroemia indica and / or Acanthus ilicifolius are planted in the third zone.The method improves the plant survival rate, stabilizes the community structure, realizes the dual effects of ecological restoration and protection, breaks the curse of low-tide flat non-habitat forest, and promotes the ecological protection and sustainable use of low-tide flat region.

The invention discloses a tissue culture and rapid propagation culture medium and a culture method of a crape myrtle gold leaf variety 'Fengjin', and belongs to the technical field of plant propagation. The method comprises the following steps: taking annual twigs of a crape myrtle gold leaf variety 'Fengjin' as explants; the method comprises the following steps: cutting the seedlings into 3-4cm stem segments with axillary buds, and inducing the stem segments through an induction culture medium for 20 days to differentiate regenerated seedlings with the regeneration rate of 72.21-79.93%; through a proliferation culture medium, proliferation can be realized within 20 days, and the proliferation coefficient is 6.17-7.71; through a rooting culture medium, tissue culture seedlings with the length of 3 cm or above can root after 20 days, and the rooting rate is 100%. A hormone combination of 0.25 mg / L of NAA and 1.00 mg / L of 6-BA is adopted in the induction culture and the multiplication culture; exogenous IBA and NAA do not need to be added in rooting culture. The tissue culture and rapid propagation optimization system of the crape myrtle gold leaf variety 'Fengjin', established by the invention, can realize rapid, efficient and stable in-vitro propagation of the crape myrtle gold leaf variety 'Fengjin'. According to the technology, the limitations of long period, low propagation coefficient, inconsistent hereditary characters and the like of a traditional propagation mode are effectively overcome, and reliable technical support is provided for large-scale seedling raising and commercial application of the crape myrtle gold leaf variety 'Fengjin'.

This invention provides the LfiGAI1 gene of Crape Myrtle and its application in regulating plant height, belonging to the field of molecular biotechnology. The LfiGAI1 gene can inhibit the division and elongation of Crape Myrtle cells, thereby regulating plant height. Overexpression of this gene in tobacco can shorten the internode length of overexpressing lines and significantly reduce plant height, indicating that GAI1 can inhibit Crape Myrtle cell elongation and thus regulate plant height, which is of great significance for the breeding of plant varieties with specific plant heights.

The invention discloses a plant growth regulator for prolonging the flowering phase of garden flowers and a preparation method and application thereof, and belongs to the technical field of plant growth regulators. The plant growth regulator comprises theaflavin, huperzine B, elaeagnus pungens leaf oil, salicylic acid, calcium chloride and 6-benzylaminopurine. The theaflavin, the huperzine B and the elaeagnus pungens leaf oil are innovatively introduced and compounded with the salicylic acid, the calcium chloride and the 6-benayl aminopurine to form the plant growth regulator, and through the synergistic effect of various substances, on one hand, cell membranes and petal tissues can be protected against oxidative damage, and petal withering and discoloration are delayed; the flowers are helped to maintain physiological activities for a longer time; on the other hand, flower bud differentiation and flower development can be promoted, early flowering is promoted, and the flowering time is prolonged. The plant growth regulator effectively promotes early flowering of crape myrtle, Chinese rose and magnolia liliflora plants in gardens, prolongs the flowering period of flowers and improves the ornamental period of the flowers.