The invention discloses a preparation method of a sterilization protection brush for a shopping mall escalator, and relates to the technical field of elevator materials; the sterilization protection brush for the shopping mall escalator comprises the following components: attapulgite, charcoal ash, turpentine oil, paraffin, trisodium phosphate, ethanol, deionized water, propylene asphalt resin, analkali solution, sucrose cocoate, Broussonetia papyrifera bark powder, Pteroceltis tatarinowii bark powder, reed straw powder, an adhesive, a stabilizer, carbohydrase, blood enzyme, an active assistant, polyethylene wax, stearic acid, butadiene-vinylpyridine rubber, an anti-aging agent, camphor, staphylococcus aureus lysozyme, superoxide dismutase, lysozyme, matrigel and a 0.1 mol / l phosphate buffer solution; a traditional elevator protective brush is optimized to obtain the sterilization protection brush, the preparation method is simple, the process is environmentally friendly, and the cost is saved. The active assistant and biological active substances are added to the sterilization protection brush to continuously remove bacteria in the environment, and can also play an elevator protective effect, and double effects can be achieved, and a cleaner indoor environment is brought.