The invention discloses a steam curing-free high-strength cement-wood chip plate and a preparation method thereof. The steam curing-free high-strength cement-wood chip plate comprises, by weight, 60-100 parts of cement, 20-60 parts of an active admixture, 10-12 parts of wood chips, 0.8-1.2 parts of thermal insulation aggregate, 7-9 parts of ceramsite, 0.3-0.5 parts of short fibers, 0.3-0.5 parts of an auxiliary agent and 45-50 parts of water. The preparation method comprises mixing cement, the active admixture, short fibers, water and the auxiliary agent to obtain a uniform mixture, adding wood chips, thermal insulation aggregate and ceramsite into the mixture, carrying out stirring to obtain a uniform mixture, laying a net, carrying out rolling flattening, then carrying out molding, loading a vehicle with plate blanks, transporting the plate blanks into a primary curing kiln, carrying out demolding, carrying out stacking, carrying out secondary curing, and naturally drying the plates so that water content is in a range of 5-10%. The steam curing-free high-strength cement-wood chip plate has excellent integrated physical properties and completely satisfies the national industry standard JC/T 411-2007 of cement-wood chip plate.