47 results about "Metaphase chromosome" patented technology

A system and method for computer-assisted karyotyping includes a processor which receives a digitized image of metaphase chromosomes for processing in an image processing module and a classifier module. The image processing module may include a segmenting function for extracting individual chromosome images, a bend correcting function for straightening images of chromosomes that are bent or curved and a feature selection function for distinguishing between chromosome bands. The classifier module, which may be one or more trained kernel-based learning machines, receives the processed image and generates a classification of the image as normal or abnormal.

Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods and reagents are provided for the detection of genetic rearrangements. Probes and test kits are provided for use in detecting genetic rearrangements, particularly for use in tumor cytogenetics, in the detection of disease related loci, specifically cancer, such as chronic myelogenous leukemia (CML), retinoblastoma, ovarian and uterine cancers, and for biological dosimetry. Methods and reagents are described for cytogenetic research, for the differentiation of cytogenetically similar but genetically different diseases, and for many prognostic and diagnostic applications.

The invention provides a fluorescence in situ hybridization method for zanthoxylum armatum metaphase chromosome. The fluorescence in situ hybridization method comprises the steps of zanthoxylum armatum metaphase chromosome glass slide specimen preparation, fluorescence in situ hybridization, signal detection and glass slide recovery. The fluorescence in situ hybridization method disclosed by the invention is beneficial to establishing a stable and convenient metaphase chromosome fluorescence in situ hybridization reaction system, greatly improves the detection efficiency of the zanthoxylum armatum metaphase chromosome and provides a novel method for detecting the anthoxylum armatum chromosome and even zanthoxylum species chromosome.

The invention provides a method for rapidly preparing metaphase chromosomes of marine valuable and rare fishes or shellfishes, and belongs to the technical field of marine organisms. The method comprises the steps that 1, test fishes or shellfishes are temporarily cultured, and glass slides are cleaned; 2, after it is guaranteed that the survival state of the test animals is good, the fishes or shellfishes of which fin rays or grills have wounds are selected to be independently cultured; 3, healing and proliferating tissue at the wounds of the test animals is taken to be subjected to chromosome preparation. The method for preparing the metaphase chromosomes is simple and rapid and convenient to operate, the vigorous division and proliferation capacity of the healing and proliferating tissue is fully utilized, it is basically guaranteed that the test animals still can be healthy and be survived after chromosome preparation, multiple times of preparation can be repeated, the needed test animal can be least to one individual, and the method is suitable for various marine valuable and rare fishes and shellfishes. According to the method, freshness of the experiment samples can be guaranteed, the cell activity is good, the defects that most of the valuable and rare fishes or shellfishes are in a mature or aged state, and cell division is not vigorous are overcome, the prepared metaphase chromosomes are good in morphology and dispersion degree, and therefore observation and counting are convenient.