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Culture medium for amniotic fluid and chorionic villus

A culture medium and basal medium technology, applied in the field of cell biology, can solve the problems of few mitotic phases, ineffective use of amniotic fluid cell inspection, low success rate, etc., to increase the clone formation rate, facilitate product scale, and clone growth The effect of speed increase

Inactive Publication Date: 2012-08-01
HUIZHOU HONGYU TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the success rate of this medium is lower than 30%, and the average culture period is 20 days, and there are few mitotic phases, so it cannot be effectively used for amniocyte inspection; in order to shorten the culture period of amniocytes and improve the success rate, an improved type has been developed. The research report of the culture medium is basically composed of extracts containing growth factors added on the basis of the culture medium

Method used

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  • Culture medium for amniotic fluid and chorionic villus
  • Culture medium for amniotic fluid and chorionic villus
  • Culture medium for amniotic fluid and chorionic villus

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0042] Prepare HEPES / NaHCO with a concentration of 20mmol / L 3 buffer system

[0043] Take 4.76g HEPES and dissolve in 900ml water for injection, and use 1.5~3g NaHCO 3 Adjust the pH of the aqueous solution to 7, then dilute to 1000ml with water for injection, and store at 4°C.

preparation example 2

[0045] Preparation of Penicillin and Streptomycin Double Antibody Stock Solution

[0046] a) Take 1 bottle of penicillin, 1 million units, and add water to dilute it to 200,000 units / ml.

[0047] b) Take a bottle of 800,000 units of streptomycin and dilute it with water to 200,000 units / ml.

[0048] c) Mix a and b in equal proportions and store at 4°C.

Embodiment 1

[0049] Embodiment 1 (A-1 medium):

[0050] Take 1L of DMEM / F12 cell culture medium, dissolve it with 950ml of water for injection, add 10mmol HEPES / NaHCO3 buffer, add 50,000 units of fibroblast growth factor, 10,000 units of epidermal growth factor, 40,000 units One unit of lymphocyte growth factor, 0.3 g of ascorbyl glucoside, 50,000 units of penicillin, and then dilute to 1000 ml with water for injection.

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Abstract

The invention relates to a culture medium for amniotic fluid and chorionic villus, and belongs to the field of cytobiology. The culture medium for amniotic fluid and chorionic villus comprises the following components: basic culture mediums, fetal calf serums, growth factors, antioxidants, antibiotics and a buffer system. Through the invention, the cost can be lowered, the cloned number and the cell proliferation speed of amniotic fluid and chorionic villus proliferated in vitro can be effectively increased, the culture period is shortened, and the culture mediums for amniotic fluid and chorionic villus with a few of metaphase chromosomes and a formula of the culture mediums for amniotic fluid and chorionic villus are obtained.

Description

technical field [0001] The invention relates to a culture medium for amniotic fluid and villi cells, belonging to the field of cell biology Background technique [0002] In recent years, due to the increasing environmental pressure, the incidence of genetically related diseases in the population has increased significantly. Preventing the occurrence of severe congenital malformations and genetic diseases and ensuring the health and prosperity of human offspring is an important task of genetics and medicine. At present, prenatal diagnosis mainly includes the collection of villous tissue in the first trimester, amniotic fluid and umbilical cord blood in the second trimester, separation of fetal cells from peripheral blood of pregnant women, and preimplantation diagnosis. However, because amniocentesis is safe to operate, the pregnancy loss rate is lower than the spontaneous abortion rate, and the diagnosis of chromosomal abnormalities is more reliable, amniotic fluid cell exa...

Claims

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Application Information

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IPC IPC(8): C12N5/073
Inventor 李锦明秦清和
Owner HUIZHOU HONGYU TECH
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