78results about How to "Morphologically normal" patented technology

The present invention provides an introduction method of the laminaria japonica, which can resolve the problems of the prior art that the selected frond section having the mature sporangia is easy to decay and the spore is easy to release, thereby leading to the failure of the introduction. The introduction method adopted by the present invention is to firstly dry and incite the mature sporangia leaf of the laminaria japonica in a temperature-controllable laboratory, then put the mature sporangia leaf of the laminaria japonica into a water tank that is provided with the fresh sterilized sea water and the temperature of the water is controlled between eight cent degrees and twelve cent degrees to release the spores, then cultivate the spore to form the gametophyte, to put the seedling curtain attached with the gametophyte into a low-temperature enclosed bag and finally transport in low-temperature storage to the destination. The shape of all gametophytes on the laminaria japonica seedling curtain is normal, which has high successful rate and the survival rate is nearly one hundred percentages.

The invention provides a method for maintaining the survival and/or function of vascular endothelial cells in the in vitro preservation process and used preserving fluid. The method and the preservingfluid are suitable for storing various organs. The in vitro liver can survive to exceed 24h during the preservation by the organ preserving liquid and in the mechanical filling process; the liver microcirculation structure is complete; no obvious damage exists; the choleresis function is good; the liver synthesis and metabolism functions are good; the in vitro preservation time of the liver is greatly prolonged; the postoperative survival rate of later-period liver transplantation patients is improved.

The invention relates to an application of ligustilide in preparing a composition for preventing and treating senile dementia, which comprises ligustilide as an effective component and other components acceptable pharmaceutically and/or acceptable in food or auxiliary components. As required, the effective components can comprise at least one of acetylcholinesterase inhibitor, excitatory amino acid and peptide transmitter components, Abeta fibration and sedimentation disturbance components, antioxidant, microcirculation improving components and calcium antagonist accounting for 10%-40% of the total weight. Shown by experimental results, through the protective actions of the ligustilide on damage caused by oxidation, apoptosis and inflammatory reaction induced by the Abeta amyloid peptide fragment and aging neuron degenerative change, the composition can obviously improve the descending of learning and memory caused by the Abeta amyloid peptide fragment and the aging neuron degenerative change, and can be used as a medicine and/or a health food composition.

The invention relates to a heat treatment method for a U71MnH steel rail flash welding joint, and belongs to the technical field of steel rail welding. The problem that harmful structures such as martensites are generated to influence the joint hardness due to the fact that the local cooling speed is higher than the critical transition temperature when an existing steel rail welding joint is cooled in the heat treatment process is solved. The heat treatment method for the U71MnH steel rail flash welding joint comprises the steps that a U71MnH steel rail welding joint welding seam area which iscooled to 300 DEG C or below after being welded is heated to 870-930 DEG C, then heating is stopped, air spraying cooling is adopted and stopped after the steel rail joint tread temperature is lowered to 420-470 DEG C, and natural cooling is conducted till the room temperature is achieved. By adopting the method, it is guaranteed that the joint structure is normal while the hardness of the U71MnHsteel rail flash welding joint is effectively controlled.

The invention relates to a heat treatment method for a U71MnH steel rail welding joint, and belongs to the technical field of steel rail welding. The problem that harmful structures such as martensites are generated to influence the joint hardness due to the fact that the local cooling speed is higher than the critical transition temperature when an existing steel rail welding joint is cooled in the heat treatment process is solved. The heat treatment method for the U71MnH steel rail welding joint comprises the steps that a U71MnH steel rail welding joint welding seam area which is cooled to 300 DEG C or below after being welded is heated to 915-935 DEG C, then heating is stopped, air spraying cooling is adopted and stopped after the steel rail joint tread temperature is lowered to 405-432DEG C, and natural cooling is conducted till the room temperature is achieved. By adopting the method, it is guaranteed that the joint structure is normal while the hardness of the U71MnH steel railflash welding joint is effectively controlled.

A vitrifying freeze storage method for the embryos of fish, especially the bastard halibut, is disclosed, which features that the formula of vitrifying liquid suitable for the embryo of seawater fish is screened out, the embryo development stage suitable for vitrifying it is determined, a particular eluting solution is disclosed, and the step balance and the fast cooling and thawing techniques are used. Its advantages are high safety, easy operation and low cost.

The invention provides a reagent capable of preventing and eliminating pollution of mammalian cells, and belongs to the field of microbial pollution control in mammalian cell culture. A polypeptide having a sequence of SEQ ID NO:1 can be used for preventing and eliminating pollution of the mammalian cells; the reagent is composed of the polypeptide having the sequence of SEQ ID NO:1 and amino acid and does not contain any antibiotic component. The product is prepared in accordance with the concentration required for prevention and elimination of pollution, microbial pollution in the mammalian cells can be effectively prevented and eliminated, and the defects of use restriction of antibiotic components in a conventional product and longer cell treatment period are overcome; and the product is good in stability and easy to preserve.

The invention discloses a bone repair material able to guide bone tissue regeneration. The material is a composition of calcined bone powder with different particle sizes and a bioabsorbable high polymer material. The bone repair material is characterized in that: the particle sizes of the calcined bone powder range from 0.1-2mm; and the bioabsorbable high polymer material is one or more of collagen, chitin, chitosan, hyaluronic acid, chondroitin sulfate, calcium alginate, fibrous protein, and elastin. The bone repair material provided in the invention has an obvious bone healing guiding effect and good biocompatibility. Also with certain hardness and strength, the bone repair material is easy to shape and has rich sources, thus solving the making problems of artificial synthetic materials in the aspects of porosity, pore traffic and pore size, etc.

The invention relates to a polyploidy induction method for fiber linum usitatissimum under an isolated culture condition, wherein the method comprises the following steps of: selecting health and full fiber linum usitatissimum seeds; sterilizing the seeds and then culturing into seedling; processing the stem tip with colchicines to induce polyploid; transforming into a stem tip extending culture media for culturing; identifying the ploidy of the stem tip chromosome; culturing the stem section which is identified to be a tetraploid plant in a callus and bud induction culture medium to obtain lots of tetraploid sterile seedlings; and then transforming to an extending growing culture medium to grow; performing rooting culture in a rooting culture medium, and then transferring to a sterilized substrate for manging; and finally identifying the ploidy of chromosome of the root tip. The method can obtain higher inductivity than that of a method which processes the fiber linum usitatissimum seeds; the inductivity can be up to 25.5%; the pure tetraploid ratio is high; the polyploidy plant has normal shape, and high transplanting survival rate; the method can realize quick breeding under the isolated condition to obtain a large amount of tetraploid tissue-cultured seedlings in a short time.

The invention relates to a traditional Chinese medicament for treating hepatitis and cirrhosis ascites which is prepared from the following raw materials (by weight portion): red ginseng 60-100 parts, white atractylodes rhizome 50-80 parts, borneol 30-50 parts, chicken's gizzard-skin 30-50 parts, red peony root 20-30 parts, blood clam shell 80-100 parts, centipede 40-50 parts, ligumaloes 20-30 parts, earthworm 80-100 parts and polyporus umbellatus 55-80 parts. The medicament is prepared through mixing, grinding into fines, sieving, and can be prepared into powders, tablets, capsules or pills.

The invention specifically relates to a method for acquiring a suspension cell line with the hypocotyl of cabbage type rape as explant and application of the suspension cell line, belonging to the technical field of plant cell culture. According to the invention, the aseptic hypocotyl of cabbage type rape is used as explant and subjected to induction of embryonic callus, culture of suspension cells and the like so as to construct the suspension cell line of the cabbage type rape; and the suspension cell line is used for research on and comparison of the different morphologies and activities ofsuspension cells under the conditions of normal supply of essential micronutrient elements and shortage of the essential micronutrient elements. The prepared suspension cells of the cabbage type rapeare high in activity, stable and uniform in morphology, short in culture period, not limited by natural environmental factors like season and climate, and applicable to research on the cell development of the cabbage type rape at a single cell level and the cell growth, morphology, transmembrane nutrient transport, physiological and biochemical metabolism and gene expression of the cabbage type rape under the condition of abiotic stress.

The invention relates to a culture medium in the technical fields of fermentation engineering and biology, in particular to a nutrition-enhanced culture medium for preparing 2-KGA through fermentation, and the nutrition-enhanced culture medium comprises a conventional culture medium and a nutrition enhancer, wherein sorbitol or sorbose is used as a substrate of the conventional culture medium. Meanwhile, the invention also relates to a method for preparing the 2-KGA by applying the enhanced culture medium. The nutrition-enhanced culture medium can promote the growth of small bacteria, enablesthe small bacteria to be disengaged from the dependence on associated bacteria, establishes independent growth and provides a foundation for the improvement of a 2-KGA fermentation process.

The invention relates to a compound preparation of montmorillonite. The compound preparation is characterized by being prepared by adding a zinc salt into montmorillonite, wherein the weight ratio of the montmorillonite to the zinc salt is 1:(0.0016-0.044). The compound preparation is used for treating diarrhea, peptic ulcer and zinc deficiency, and has the advantages of no side effects, simple production process and good effects for treating diarrhea, peptic ulcer and zinc deficiency.

The invention relates to the field of economical brown kelp seaweed varietal complexity, in particular to a method for detecting varietal complexity in kelp summer seedling refrigeration water process. The method comprises the steps that a seedling rope which is inoculated with a female gametophyte clone is put in a culture medium or a kelp seedling refrigeration water circulation system to be cultured, parthenogernesis sporophore is obtained when the seedling rope is put in the culture medium, and offspring sporophore is obtained when the seedling rope is put in the kelp seedling refrigeration water circulation system; morphological characteristics and genetype of the offspring sporophore on the seedling rope in the refrigeration water circulation system are detected and compared with those of the parthenogernesis sporophore so as to determine whether varieties are hybrid or not in the seedling process. According to the detection method, it is verified for the first time that in the kelp summer seedling production process, due to the fact that low temperature circulation water is used in common, the varietal complexity is definitely caused.

The invention belongs to the technical field of tissue culture, and relates to a tissue culture and rapid propagation research method suitable for industrialized production. The method is summarized and concluded on the basis of experimental research and production for two years on the basis of a new variety of caladium bicolor introduced by Thai, has strong operability and can be completely used for guiding production. According to the invention, the complete rapid induction, multiplication, rooting and seedling hardening propagation method which has high inductivity and propagation multiple and is suitable for industrial production is established for the first time, a batch rapid propagation situation is formed, and the propagation generations, the hormone concentration of a culture medium and the proportion are controlled to change along with the change of the generations, stability, activity and the proliferation coefficient of seedlings are ensured, and the yield and quality are improved; and according to the method, a production manner of simultaneously propagating and rooting is adopted, so that the production cost is reduced, the production progress is accelerated, the propagation coefficient is ensured, the quality of rooted seedlings is ensured, the method is completely suitable for large-scale industrialized production, and the breeding efficiency of the caladium bicolor is greatly improved.

The invention discloses a design method of a large-curvature cable-membrane structure, and aims to solve the technical problems that the existing cable-membrane structure is easy to generate larger deformation to change the shape size or loosen, and the analysis and design theory of the cable-membrane structure at home and abroad is not completely mature. The design method comprises the following steps: (1) carrying out performance analysis on a membrane material to be used by the large-curvature cable membrane structure; (2) adopting ANSYS software to carry out shape finding analysis on the large-curvature cable membrane structure; (3) selecting a membrane material with a proper thickness; (4) optimizing a reinforcing cable net in the large-curvature cable membrane structure; (5) carrying out technical verification and hardware optimization on the large-curvature cable membrane structure; and (6) designing proper light transmittance of the membrane material. According to the design method, a collaborative stress system of the steel cable, the membrane material, the hardware and the cable membrane support steel structure is optimized, the overall stress stability of the structure and the applicability of the component are improved on the premise that the material performance is brought into full play, and the building body art is perfectly expressed.

The invention discloses an infection enhancing culture medium and method for improving the cell lentivirus infection rate, and relates to the field of MDA-MB-231 cell transfection. The enhanced infection culture medium is used for improving the lentivirus infection rate of the MDA-MB-231 cell, and the enhanced infection culture medium is prepared from the following components: a Leibovitz's L-15 culture medium, an RPMI-1640 culture medium, fetal calf serum, non-essential amino acid and an epidermal growth factor; according to the volume percentage of the total raw materials, the addition amount of the fetal calf serum is 10%, and the addition amount of the non-essential amino acid is 1%; the addition amount of the epidermal growth factor is 2-3ng/ml. By adopting the enhanced infection culture medium disclosed by the invention, the MDA-MB-231 cells can be normally cultured in the conventional environment of 37 DEG C and 5% CO2, and the infection efficiency of the lentivirus on the MDA-MB-231 cells is more remarkably improved, so that the gene editing efficiency of the MDA-MB-231 cells becomes higher, and the problem that the lentivirus infection rate of the MDA-MB-231 cells is low at present is solved.

The invention provides a human chondrocyte culture medium, and belongs to the technical field of cells. The invention optimizes a traditional culture medium, and by adding exosomes, fetalbovine serumand penicillin-streptomycin into a basal culture medium, chondrocyte proliferation can be effectively promoted, dedifferentiation of human chondrocytes is prevented, transformation of the human chondrocytes into fibrocyte-like phenotypes is prevented, and the single-layer culture passage number is increased. The invention also provides a method for isolating culture of the human chondrocytes by using the above-mentioned culture medium, and normal chondrocytes cultured to the sixth passage can be obtained.