A kind of mesenchymal stem cell preservation solution and its preparation method and application

Abstract

The invention belongs to the field of biomedicine and discloses a mesenchymal stem cell preservation solution and its preparation method and application. The mesenchymal stem cell preservation solution contains human AB type umbilical cord blood plasma and 70- 80AXaIU / ml calcium low molecular weight heparin; the human AB type umbilical cord blood plasma and mesenchymal stem cells come from the same provider. The mesenchymal stem cell preservation solution of the present invention can prolong the activity maintenance time of mesenchymal stem cells (maintain more than 90% of the activity for 72 hours at 4-15°C), and the mesenchymal stem cells are stored in the preservation solution of the present invention for 72 hours. , the cell morphology is normal, does not affect its proliferation and expansion ability, and does not affect the phenotypic characteristics of mesenchymal stem cells. The mesenchymal stem cell preservation solution of the present invention is safe and reliable in clinical application, and the AB-type umbilical cord blood plasma and umbilical cord mesenchymal stem cells are derived from the same placenta and belong to autologous plasma, which reduces the possibility of exogenous virus contamination.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesenchymal stem cell preservation solution and a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (mesenchymal stem cells, MSCs) are a kind of stem cells derived from mesoderm with multi-lineage differentiation ability, which widely exist in various tissues of the body, can be cultured and expanded in vitro, and can differentiate into cardiomyocytes under specific conditions , nerve cells, and fat cells. [0003] With the in-depth research on the biological characteristics and functions of mesenchymal stem cells by scholars at home and abroad, mesenchymal stem cells can be isolated from various tissues. Mesenchymal stem cells can support hematopoiesis and regulate immunity, and have broad clinical application prospects in hematopoietic reconstruction, tissue repair, and immunotherapy. Mesenchymal stem cells do not express...

AI Technical Summary

Problems solved by technology

[0008] But one of the shortcomings of the existing technology is that TC199 in the mesenchymal stem cell preservation solution is a cell culture medium, which is usually used for cell and tissue culture, and is not a nationally approved reagent that can be clinically applied to the human body. Complicated, the quality of culture media from different manufacturers varies, and imported reagents are expensive. In recent years, with the continuous development of the field of biological reagents, TC199 has gradually withdrawn from the media market and has been replaced by other media, so its source is limited

[0009] In short, although the existing technology uses TC199 as an active ingredient in the mesenchymal stem cell preservation solution to maintain cell viability for 24 hours, it is not conducive to the clinical promotion and use of mesenchymal stem cells in terms of reagent source, clinical application legitimacy, and reagent cost. of

[0010] Another disadvantage of the prior art is that it maintains the viability of mesenchymal stem cells for a short period of time. Mesenchymal stem cells can maintain more than 90% of their viability in the cell preservation solution of the prior art for only 24 hours, and with time Prolonged, the cell viability decreases, which means that after 24 hours, the cell viability will drop below 90%. However, stem cell therapy and clinical trials have been carried out in most cities in China and many foreign countries. Using existing means of transportation, 24 hours The viability maintenance time of mesenchymal stem cells obviously limits the application range of mesenchymal stem cells

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