Preserving solution used for cells after cryopreservation resuscitation and application of preserving solution

A preservation solution and cell technology, applied in the biological field, can solve the problem of low cell survival rate, achieve the effect of increasing the amplification factor, increasing the survival rate, and enhancing the stability

Inactive Publication Date: 2019-06-07
CENTURY BIOSTRENGTH BEIJING PTY LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems in the prior art that the cell membranes in the conventional preservation solution are weak and cause the cell survival rate to be low after cryopreservation and resuscitation, the present invention provides a cell preservation solution for cryopreservation and recovery, which prolongs the time of cryopreservation. The activity maintenance time of recovered cells can reduce their pollution rate and increase their survival rate

Method used

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  • Preserving solution used for cells after cryopreservation resuscitation and application of preserving solution
  • Preserving solution used for cells after cryopreservation resuscitation and application of preserving solution
  • Preserving solution used for cells after cryopreservation resuscitation and application of preserving solution

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Embodiment 1

[0027] A cell preservation solution used for cryopreservation and resuscitation, the preservation solution is mainly formed by dissolving ammonium acetate and total ginsenosides with a concentration of 40mg / mL MEM medium aqueous solution, wherein the concentration of ammonium acetate after dissolution is 25mg / mL mL, the concentration of total ginseng saponins after dissolution is 20mg / mL.

Embodiment 2

[0029] A cell preservation solution used for cryopreservation and resuscitation, the preservation solution is mainly formed by dissolving ammonium acetate and total ginsenosides with a concentration of 45mg / mL MEM medium aqueous solution, wherein the concentration of ammonium acetate after dissolution is 30mg / mL mL, the concentration of total ginseng saponins after dissolution is 25mg / mL.

Embodiment 3

[0031] A cell preservation solution used for cryopreservation and resuscitation, the preservation solution is mainly formed by dissolving ammonium acetate and total ginsenosides with a concentration of 55mg / mL MEM medium aqueous solution, wherein the concentration of ammonium acetate after dissolution is 32mg / mL mL, the concentration of total ginseng saponins after dissolution is 28mg / mL.

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Abstract

The invention provides a preserving solution used for cells after cryopreservation resuscitation and an application of the preserving solution. The preserving solution is mainly formed by dissolving ammonium acetate and total ginsenoside into an MEM culture medium aqueous solution with a concentration of 40-55 mg / mL, wherein the concentration of the dissolved ammonium acetate is 25-32 mg / mL, and the concentration of the dissolved total ginsenoside is 20-28 mg / mL. The preserving solution provided by the invention provides sufficient nutrients for the cells after the cryopreservation resuscitation, maintains the survival rate, reduces the contamination rate and prolongs the cryopreservation time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cell preservation solution used for cryopreservation and recovery and its application. Background technique [0002] Cell therapy refers to using the characteristics of certain cells with specific functions, using bioengineering methods to obtain and / or through in vitro expansion, special culture, etc., to make these cells have the ability to enhance immunity, kill pathogens and tumor cells, and promote tissue regeneration. Therapeutic effects such as organ regeneration and body rehabilitation, so as to achieve the purpose of treating diseases. [0003] The treatment range of cell therapy includes: chronic diseases, such as chronic renal failure, digestive disorders with decreased function of stomach, gallbladder and pancreas, chronic constipation, chronic liver failure, intervertebral disc damage, spinal pain, premature loss of joints, etc.; degenerative diseases, Such as ataxia,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 董明珠李霞云潘新刘世红卢家堃
Owner CENTURY BIOSTRENGTH BEIJING PTY LTD
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