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Fresh amnion preservation fluid, fresh amnion preservation method and application thereof

A technology for preserving fluid and amniotic membrane, which is used in applications, preservation of human or animal bodies, animal husbandry, etc.

Inactive Publication Date: 2009-06-03
天津市医药科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest existing problem is the preservation of fresh amniotic membrane

Method used

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  • Fresh amnion preservation fluid, fresh amnion preservation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the preparation of fresh amniotic membrane preservation solution

[0047] Precisely weigh the various substances in the formula, carry out in a sterile state according to the method and operating procedure of the medical tissue preservation solution, and pay attention to maintaining the pH value (6.8-7.4) of the preservation solution suitable for the physiological needs of the cells. May contain the following substances:

[0048] 1. 9.0 grams of DMEM medium

[0049] 2. Chondroitin Sulfate 10.0g

[0050] 3. Sodium hyaluronate 0.5g

[0051] 4.HEPES 4.0g

[0052] 5. Low molecular weight dextran 10.0g

[0053] 6. Gentamicin 1.0ml (40,000 units / ml)

[0054] 7. Dexamethasone 24.0 mg

[0055] 8. Non-essential amino acids 10.0ml

[0056] 9. Reduced glutathione 0.50g

[0057] 10. Fetal bovine serum 20.0ml

[0058] 11. Add water for injection to 1000 ml.

[0059] Electron micrograph after above-mentioned solution preservation 30 days, as figure 1 As shown...

Embodiment 2

[0061] Precisely weigh the various substances in the formula, carry out in a sterile state according to the method and operating procedure of the medical tissue preservation solution, and pay attention to maintaining the pH value (6.8-7.4) of the preservation solution suitable for the physiological needs of the cells. It can also be this content ratio:

[0062] 1. 12.0 grams of DMEM medium

[0063] 2. Chondroitin Sulfate 25.0g

[0064] 3. Sodium Hyaluronate 1.0g

[0065] 4.HEPES 5.0g

[0066] 5. Low molecular weight dextran 15.0g

[0067] 6. Gentamicin 3.0ml (40,000 units / ml)

[0068] 7. Dexamethasone 30.0 mg

[0069] 8. Non-essential amino acids 15.0ml

[0070] 9. Reduced glutathione 0.95g

[0071] 10. Fetal bovine serum 50.0ml

[0072] 11. Add water for injection to 1000ml

Embodiment 3

[0074] Precisely weigh the various substances in the formula, carry out in a sterile state according to the method and operating procedure of the medical tissue preservation solution, and pay attention to maintaining the pH value (6.8-7.4) of the preservation solution suitable for the physiological needs of the cells. It can also be formulated with the following contents:

[0075] 1. 12.0 grams of DMEM medium

[0076] 2. Chondroitin Sulfate 25.0g

[0077] 3. Sodium Hyaluronate 1.0g

[0078] 4.HEPES 5.0g

[0079] 5. Low molecular weight dextran 15.0g

[0080] 6. (Antibiotic) Gentamicin 3.0ml (40,000 units / ml)

[0081] 7. Dexamethasone 30.0 mg

[0082] 8. Non-essential amino acids 15.0ml

[0083] 9. Reduced glutathione 0.95g

[0084] 10. Add water for injection to 1000 ml.

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Abstract

The invention discloses a fresh amnion preservation fluid, a fresh amnion preservation method and application thereof. The fresh amnion preservation fluid is made by adding water for injection to 9.0-12.0g of DMEM culture medium, 10.0-25.0g of chondroitin sulfate, 0.5-1.0g of sodium hyaluronate, 4.0-5.0g of HEPES, 10.0-15.0g of dextran, 1.0-6.0ml of gentamycin, 24.0-30.0mg of dexamethasone, 10.0-15.0ml of non-essential amino acid, 0.50-0.95g of glutathione, 0.0-50.0ml of fetal calf serum to 1000ml. The fresh amnion preservation method comprises the following steps: separating the amnion by a blunt, rinsing the amnion clean, and then processing the amnion by an antibiotic; cutting and packaging the amnion into a container with the amnion preservation fluid; storing the amnion preservation fluid in a refrigerator with the temperature of 4 DEG C, and rinsing hands clean with aseptic phosphate buffer before use. The fresh amnion preservation fluid has the following advantages: 1. the amnion preservation fluid is only stored in the refrigerator with the temperature of 4 DEG C; 2. the shelf life is prolonged to at least 20-30 days; 3. the amnion preservation fluid can be in batch processing and centralized storage, and 4. the amnion preservation fluid can be applied to various amnion transplantation repairs, for example (1) ocular surface reconstruction as a conjunctival substitute, (2) amnion transplantation for treating symblepharon, and (3) treatment of corneal ulcer caused by various reasons and the like.

Description

Technical field: [0001] The invention relates to a preservation method and application of medical biomaterials from amniotic membrane, in particular to fresh amniotic membrane tissue, which still has the characteristics of fresh amniotic membrane after being preserved in a preservation solution, and is especially suitable for use in the repair and treatment of eye diseases. Background technique: [0002] Amniotic membrane is the innermost layer of human fetal membranes, a kind of translucent tissue with certain toughness without blood vessels, nerves and lymphatic vessels. As a medical biomaterial, amniotic membrane has been used clinically to repair skin ulcers and eye disease injuries. In recent years, amniotic membrane transplantation has become a hot spot in research and application. As a new technique of ocular surface reconstruction, amniotic membrane transplantation has been widely used clinically and achieved good clinical results. [0003] The clinical effect of am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 肖光礼张国庆吴亮聂卫种影影高萍祝君梅
Owner 天津市医药科学研究所
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