12 results about "Tissue Preservation" patented technology

Featured here are methods for improving tissue or organ preservation and utilization through mitochondrial transplantation.

The present application belongs to the technical field of tissue transportation, and particularly relates to a tissue preservation and transportation device and a tissue preservation reagent, which aims to solve the technical problem that the activity of living tissue is reduced or the living tissue is dead after transportation in the prior art, and it is difficult to retain the original tissue characteristics. The tissue preservation and transportation device comprises a tissue preservation reagent and a preservation tube. The tissue preservation reagent comprises a temperature-sensitive gel, an antioxidant stress component and a tissue preservation solution. The preservation tube is used for containing the tissue preservation reagent. The tissue preservation and transportation device and the tissue preservation reagent utilize the tissue tube and the tissue preservation reagent to effectively buffer the jolt suffered by the tissue during transportation. The characteristics of the temperature-sensitive gel facilitate the transfer of the tissue block and the removal of the tissue block. The antioxidant stress component can keep the tissue characteristics unchanged. The tissue preservation solution can keep the tissue cell activity, and plays a good protection role on the tissue during transportation. The tissue preservation and transportation device provides a simple and effective method for tissue preservation and transportation.

The invention belongs to the field of biology, and discloses a tissue formula preserving fluid containing antimycin A. The tissue formula preserving fluid comprises a basic culture medium, the basal culture medium contains superoxide dismutase, catalase and antimycin A. On the basis of the superoxide dismutase and the catalase, the preservation solution is matched with the antimycin A, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, when the respiratory chain inhibitor drug is applied to the formula of the invention, the improvement degree of the cell viability of only antimycin A is the highest; 2, the function of antimycin A must be exerted based on the use of superoxide dismutase and catalase; meanwhile, the invention further provides a tissue preservation method.

A powder formulation for preserving tissue and organ function, and more particularly to shelf stable powder formulations that can be reconstituted at point of use, including during a fat grafting process, coronary artery bypass graft surgery, and peripheral bypass surgery, or other grafting / transplant surgery prior to or during the medical procedure to preserve the tissues / organs used in these procedures. The powder formulation includes a reducing agent; an antioxidant; a sugar; a nitric oxide substrate; a buffering agent; and physically acceptable salt. Also provided are a method of preparing an organ or tissue preservation solution by reconstituting the powder formulation, and a method of preserving tissue or an organ using same.

The invention belongs to the field of biology, and discloses a tissue sample preservation solution which comprises a basic culture medium, the basic culture medium contains fetal calf serum and MitoQ, and FBS and MitoQ are added and used on the basis of the basic culture medium, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, in the presence of FBS, only MitoQ in the mitochondrial targeting antioxidant can show a relatively strong tissue activity maintaining effect; 2, the effect of MitoQ in a basic culture medium is not fully played, and the MitoQ must be played in the presence of FBS; meanwhile, the invention further provides a tissue preservation method.

The invention belongs to the technical field of tissue engineering, cell biology and tissue preservation, and particularly relates to a serum-free adherent tissue preservation method and application of the serum-free adherent tissue preservation method in primary cell separation and amplification. The serum-free adherent tissue preservation method comprises the following steps: cutting a to-be-preserved tissue into blocks, placing the blocks in a serum-free basal culture medium, and preserving the blocks under a controlled, simulated and close-to-physiology metabolic microenvironment condition for at least 14 days. According to the technical scheme, freezing and a high-concentration cryoprotectant are not needed, operation is easy and convenient, the heterogenous pollution risk is avoided, the tissue can be stably preserved for at least 14 days, cell emigration has high controllability, the method is suitable for various scientific research and clinical scenes, the problems that in the prior art, the tissue preservation period is short, cell damage is large, operation is complex and the like are solved, and wide application prospects are achieved.

The invention relates to a method for preservation of a tissue or an organ previously obtained from a mammal, for transplant of said tissue or organ in a recipient compatible mammal, the method comprising: - contacting, preferably in vitro or ex vivo, said tissue or organ with a treating composition, the treating composition comprising a neddylation pathway inhibitor, for 1 to 12h hours, in order to obtain a treated tissue or organ, said composition comprising from 1 to 100 µM of said neddylation pathway inhibitor; - washing the treated tissue or organ with a conservation composition for removing the treating composition from the treated tissue or organ, in order to obtain a washed tissue or organ; and - contacting washed tissue or organ with a preservation composition, in order to obtain a ready to be transplanted tissue or organ.

The utility model discloses an animal tissue storage device with shockproof structure relates to animal tissue storage technical field. The utility model discloses a arc bottom plate and fixed clamping plate, arc bottom plate top is provided with fixed frame, and the outer wall of fixed frame is provided with elastic link, and fixed clamping plate sets up at elastic link one end, and the outer wall of fixed clamping plate is provided with push slide, and the inside of fixed frame is provided with adjusting sliding slot, and the inside of adjusting sliding slot is provided with adjusting slide plate. The utility model discloses setting up the protection mechanism, not only can when test tube is placed to the inside of the placing groove, the test tube is fixed with clamping, can avoid because the road surface is uneven and drives test tube and the animal tissue sample in test tube to shake, makes animal assembly sample and test tube inner wall to collide and causes animal tissue deformation, rupture or cell damage to animal assembly sample, and can adjust the clamping strength of protection mechanism to test tube according to the clamping demand of test tube, can guarantee the stability of test tube in the transportation process.

The invention discloses a preserving fluid for preserving pancreatic cancer tissues and application of the preserving fluid. The preserving fluid is prepared from mannitol, reduced glutathione, allopurinol, magnesium sulfate, gabexate mesylate, orlistat, pyruvic acid, Primocin, dexamethasone, heparin, bovine serum albumin, HEPES and the balance of an advance DMEM / F12 culture medium. The preserving fluid has a good effect in maintaining the pancreatic cancer tissue activity and inhibiting self-digestion and oxidative damage, is beneficial to maintaining the cell activity, and is beneficial to the preservation of pancreatic ductal adenocarcinoma in-vitro tissues and the construction of subsequent organoid.

A live tissue storage solution is formed of an aqueous culture medium including additives, inorganic salts and nutrients. The additives preferably include amino acids including but not limited to L-Arg, L-Cys, Gly, L-His, L-Ile, L-Lys, L-Leu, L-Met, L-Phe, L-Ser, L-Try, L-Try, and L-Val. The inorganic salts preferably include calcium chloride, sodium chloride, magnesium sulphate, sodium bicarbonate, sodium phosphate. The nutrients preferably include glucose, choline, folic acid, nicinamide, riboflavin, which are known to maintain cell culture. Examples include Dulbecco's Modified Eagle Medium DMEM or Minimal Essential Medium MEM with or without serum. The culture medium contains additives to reduce, inhibit, or mitigate lipid oxidation, lipotoxicy, lipid-induced responses, and lipid inflammation.

A tissue storage solution includes hypothermic storage compositions and methods. The hypothermic storage composition includes media containing Dulbecco's modified Eagle's medium (DMEM) and albumin. A method of hypothermically storing tissue includes storing such tissue in a storage medium including DMEM and albumin. A method for wound or defect treatment includes applying tissue, stored in a hypothermic storage medium containing DMEM and albumin, to the site of such wound or defect.

The invention discloses a kit and a method for preparing a crustacean tissue single-cell suspension, and belongs to the technical field of single-cell suspension preparation. The tissue preserving fluid and the enzymatic hydrolysate in the kit disclosed by the invention cooperate with each other, so that various technical problems of crustacean sample treatment are solved. The single-cell suspension prepared by using the kit and the method disclosed by the invention is high in motility rate and low in caking rate, and can be directly applied to high-end applications such as flow cytometry sorting, primary culture, single-cell sequencing and the like, and the success rate is greatly improved.