99 results about "Tissue Preservation" patented technology

Compositions that include nanoscale structured materials or precursors thereof (e.g., self-assembling peptides) are described. The compositions can include other substances (e.g., a vasoconstrictor). Also described are methods for using the compositions to promote hemostasis, to protect the skin or wounds from contamination, to decontaminate a site upon removal of previously applied compositions that provided a protective coating, and to inhibit the movement of bodily substances other than blood. The compositions are also useful in isolating tissue, removing tissue, preserving tissue (for, e.g., subsequent transplantation or reattachment), and as bulking, stabilizing or hydrating agents. Medical devices that include the compositions (e.g., a stent or catheter), bandages or other wound dressings, sutures, and kits that include the compositions are also described.

The present invention provides for compositions and methods for the preservation of tissues and organs ex vivo and in situ. In addition, the present invention provides for kits that may be used in the preparation of the solutions of the present invention. The present invention also provides a device for perfusing tissues and organs with the solutions of the present invention.

This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that is effective to fix the phosphoproteins, and that has a sufficient water content to be soluble for a stabilizer and/or a permeability enhancing agent); (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; and (3) a permeability enhancing agent (e.g. PEG). Methods are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. the following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample.

The present application relates to methods for increasing stability of formalin fixed, optionally paraffin embedded biological material. In one example, DNAse inhibitors and/or RNAse inhibitors are combined with the biological material or formalin at the time of fixation or shortly prior.

The invention provides an antifreeze glycolipid compounds and composition comprising a polysaccharide moiety of Formula I:

wherein D-Manp represents a D-mannopyranose moiety, D-Xylp represents a D-xylopyranose moiety, and n is about 5 to about 70; and one or more lipid moieties covalently linked to the polysaccharide moiety of Formula I or electrostatically associated with the polysaccharide moiety of Formula I. The antifreeze glycolipid compounds and compositions can be used for a variety of industrial, agricultural, medical, and cosmetic applications where recrystallization-inhibition, cyroprotection, or cryopreservation is desired. The antifreeze glycolipid compounds or compositions can be used as, for example, as cryoprotectants for tissue preservation and transplantation, improving the texture of processed frozen food and frozen meats, frostbite protection, crop protection, and green alternatives for land vehicle antifreeze and aircraft de-icing.

A tissue sample preservation process and device for improving and standardizing tissue preservation procedure, including placing tissue specimens in a cold fixative, performing fixative penetration at a refrigerated temperature, and accelerating fixative penetration by ultrasound. Also disclosed is the application of ultrasound and temperature control in the dehydration, clearing, and impregnation steps.

A method of preserving tissue by placing the tissue in a media containing pure perfluorocarbon is provided. Also provided is a tissue preserving media including storage media and pure perfluorocarbon. A method of making media by adding pure perfluorocarbon to the media is provided. A method of preserving tissue by oxygenating the storage media by adding pure perfluorocarbons to the media is also provided.

The invention discloses in-vitro tissue preserving fluid and a preparation method thereof. Each 1,000 milliliters of preserving fluid comprises the following components by weight: 5 to 200 grams of osmotic pressure regulator, 0.2 to 40 grams of a pH value buffer pair, 3 to 15 grams of potassium chloride, 1 to 5 grams of sodium chloride, 10 to 300 milligrams of antibiotic, 5 to 100 grams of glucose and the balance of deionized water. In the invention, a non-CO2 dependent buffer pair is used to reduce the dependence on CO2 in transport and an air exposure process and reduce the fluctuations of the pH value; a hypertonic and high-potassium environment is used to reduce the swelling of cells and tissues and improve the activities of the cells and tissues; and the antibiotic used is free from causing hypersensitivity reaction. The preparation method is simple and in-vitro tissue preserving fluid has a remarkable in-vitro tissue preserving effect. The in-vitro tissue preserving fluid is low in cost, has low transport requirements, and can be stored at room temperature and conveniently.

The invention relates to the field of organoid culture, in particular to a culture medium, a method and a kit for rapidly culturing tumor organoids. According to the culture medium for culturing the tumor organoids provided by the invention, the time for culturing the tumor organoids can be greatly shortened. The kit for rapidly culturing the tumor organoid comprises a culture medium for culturing the tumor organoid, a tissue preservation solution, a tissue digestion solution and a cell mass collection solution, and when the kit is used for culturing the tumor organoid, the success rate and survival rate of culturing the tumor organoid can be increased, an organoid model can be rapidly established within 2-5 days, which can be applied to subsequent experimental tests.

The invention discloses a placenta tissue matrix material. A placenta extracellular matrix is prepared by taking placenta tissue as a raw material through the technologies of tissue preservation, tissue treatment, sterilization, virus inactivation and the like, the placenta extracellular matrix retains a natural matrix structure and basic biological characteristics, and the extracellular matrix particularly retains receptors of various active factors. A preparation method comprises the following steps: tissue pretreatment; sterilization and virus inactivation; tissue DNA removal; decellularization; and preparation of various forms such as gel, sponge or powder from the acellular matrix according to specific clinical requirements. The acellular matrix disclosed by the invention is a specialscaffold material with an active factor receptor, can actively attract active ingredients in a living body into the material, is beneficial to accumulation of cell active ingredients, supports cell migration, adhesion, proliferation and differentiation, and can accelerate damaged tissue repair and local tissue reconstruction.

The invention belongs to the field of biotechnologies and relates to a method for preserving rubber tree leaf tissues extracted by DNA (deoxyribonucleic acid). The method comprises the following steps: placing collected rubber tree leaves in a pre-cooled mortar, and utilizing liquid nitrogen for grinding until the leaves become powder; and then transferring the powdery leaf tissues into a centrifugal tube containing storage buffer solution, uniformly mixing and placing in a low-temperature environment for preservation. The method which combines the storage buffer solution with low-temperaturerefrigeration is utilized for preserving the rubber tree leaf tissues, thereby effectively realizing the freshness of the rubber tree leaves for a long time, facilitating long-distance sampling, material transportation and storage, and having the characteristics of simplicity in process, low cost, good effects and the like.

The invention belongs to the technical field of detection equipment in the pathology department, and particularly to a slicing device for sample manufacturing in the pathology department. Specific tothe problems that the slicing device for sample manufacturing in the pathology department cannot be used for continuous slicing, slicing accuracy is low, consumed time is long and preservation is improper after slicing, the invention provides a scheme as follows: the slicing device comprises a mounting rack, supports are welded with the tops of outer walls on two sides of the mounting rack, a workbench is fixed on the outer walls of the tops of the supports through screws, and a sample manufacturing box is fixed at the top of an outer wall on one side of the workbench through screws. By the sample manufacturing box, subsequent samples are convenient to distribute uniformly, a magnetic head and a magnetic slicing cutter head cooperate with glass slides, paraffin blocks can be simultaneouslycut into multiple groups of slices with uniform thicknesses, the need for tissue to be sliced step by step is avoided, meanwhile, time consumed by tissue cutting is shortened, a sample slice storagebox can keep the optimum temperature for tissue preservation, and the dyeing problem caused by non-uniform slicing due to tissue unfreezing and softening is prevented.

An antifreeze glycoprotein comprising at least one C-linked saccharide according to the following general formula:

wherein X is nitrogen, sulfur, carbon or (CR¹R²)_nS═O wherein n is 0, 1, 2 or 3, Y is carbon, nitrogen, sulfur or oxygen, R¹ is hydrogen, methyl, fluorine, a carbohydrate, or a hydroxy chain, R² is hydrogen, methyl, fluorine, a carbohydrate or a hydroxy chain, R³ to R⁶ independently represent an alkyl chain, a hydroxy group, a dimethyl sulfoxy group, a carbohydrate or an ether, R⁷ is ornithine, lysine, an alkyl chain, a pyridyl group or a heterocycle; and R⁸ is hydrogen, fluorine a carbohydrate or a hydroxy or methoxy group. Such antifreeze glycoprotein analogues are useful recrystallization inhibitors and may be used as a cryoprotectant for tissue preservation and transplantation, improving the texture of processed frozen food and frozen meats, frostbite protection, crop protection, and green alternatives for land vehicle antifreeze and aircraft de-icing.

The invention relates to the field of tissue engineering technology, and in particular to a breast repair patch and a preparation method thereof. Problems in the prior that collagen bundles on the surface of a loose layer become free, floating and even falling off are solved; appearance quality of the finished product (the breast repair patch) is improved and service cycle of tissue preservation liquid and preservation cycle of the finished product are prolonged; and meanwhile, the breast repair patch, when used, can facilitate adhesion and fixation of the membrane tissue loose layer, accelerate in-growth and migration of breast cells and tissues, induce generation of new tissues and greatly relieve such symptoms as hard breasts, pain and the like after an operation. According to the embodiment, the breast repair patch provided by the invention comprises a mammal membrane tissue which comprises a dense layer and the loose layer and a collagen coating which is formed on the surface of the loose layer.

The invention belongs to medical anatomy, and particularly relates to a novel cadaver specimen preserving fluid. The novel cadaver specimen preserving fluid comprises the following components in parts by mass: 0.6-6 parts of phenoxyethanol, 0.6-6 parts of potassium sorbate, 0.3-1 part of vitamin C, 0.1-0.6 part of musk oxalic acid, 0.6-1.5 parts of sodium chloride, 0.6-1.5 parts of ferric chloride and 78-97 parts of water. The novel cadaver specimen preserving fluid is good in preservation effect, and reliable in antiseptic and anti-mold properties on the cadaver specimen; the specimen does not mould, does not shrink, is natural in color, and flexible and moist in tissue; and the preserving fluid is non-toxic, free of a bad pungent smell, free of sediment, and free of an effect on health of a worker, and does not need to be exchanged in the use procedure. The novel cadaver specimen preserving fluid is relatively ideal cadaver specimen preserving fluid in education and research at present, and is good substitute goods of formalin in long-term tissue preservation.