42 results about "Tissue Preservation" patented technology

The present invention discloses a buffer for tissue separation and / or preservation and its application, and a method for culturing tumor organoids, and relates to the field of organoid culture. The buffer is used to preserve tumor tissue samples, which are of liquid origin; the buffer includes: a basal culture medium and specific additive factors, and the specific additive factors include: superoxide dismutase, catalase, penicillin, and streptomycin; based on the volume of the basal culture medium, the concentration of superoxide dismutase is 1 to 100 μg / mL, the concentration of catalase is 50 to 500 μg / mL, the concentration of penicillin is 50 to 200 U / mL, and the concentration of streptomycin is 50 to 200 μg / mL. The tissue preservation solution of the present invention is used for the transportation and protection of liquid-derived tumor tissue samples, and has a high tumor cell activity retention rate. It is used to construct tumor organoids with a high success rate and a large number, which can meet the needs of clinical detection and disease research.

The invention discloses a tissue preservation solution in organoid construction as well as a preparation method and application thereof. The tissue preservation solution is prepared from the following components: an Advanced DMEM / F12 culture medium, a mycillin / amphotericin B three-antibody mother solution, Glutamax-1, an HEPES buffer solution and a Y-27632 mother solution. The preparation method of the tissue preserving fluid comprises the following steps: adding an Advanced DMEM / F12 culture medium into a centrifugal tube on a super clean bench; then sequentially adding a mycillin / amphotericin B three-antibody mother solution, Glutamax-1, an HEPES buffer solution and Y-27632, and uniformly mixing, so as to obtain the tissue preservation solution. After uniformly mixing, filtering and sterilizing by using a disposable needle type filter, and collecting the preserving fluid into a centrifugal tube; the invention also discloses an application of the tissue preserving fluid in organoid construction. According to the tissue preserving fluid obtained by the invention, in the transportation process, the cell viability in the tissue is improved, the cell damage is reduced, and the success rate of primary cell adherent culture and organoid construction is increased.

Featured here are methods for improving tissue or organ preservation and utilization through mitochondrial transplantation.

Disclosed herein include methods, compositions, and kits suitable for use in preservation (e.g., cryopreservation) of samples. The composition (e.g., cryopreservation reagent) can comprise: one or more cryoprotective agent(s), one or more culture media component(s), one or more carbohydrate(s), and / or one or more amino acid(s). The composition can be capable of maintaining one or more cellular properties and / or cell viability of a plurality of cells in a sample for a period of time under a storage condition. Also provided are methods of sample preservation and sample analysis. The method can comprise contacting a sample with the cryopreservation reagent, thereby generating a shielded sample. The method can comprise exposing the shielded sample to a freezing storage condition, thereby generating a cryopreserved sample.

The invention provides a cleaning and preserving fluid for fetal brain tissues and a preserving method thereof, and belongs to the technical field of tissue preservation. The cleaning and preserving fluid comprises trehalose, nerve growth factors, a GlutaMAX supplement, Y27632, S-nitrosoglutathione, tert-butylhydroquinone, a Versene solution and polyvinylpyrrolidone, compared with a traditional tissue preserving fluid, the cleaning and preserving fluid not only can improve the purity of neural stem cells, but also can maintain the activity and the yield of the neural stem cells in fetal brain tissue, and the cleaning and preserving fluid has the advantages that the cleaning and preserving fluid is simple in structure and convenient to use. The structural integrity of the cells is guaranteed, a high-quality sample is provided for subsequent research on proliferation, differentiation and the like of the neural stem cells, and the method has a wide application prospect.

A method for preserving a tissue graft includes submerging the tissue graft in a solution containing one or more antimicrobial and / or stabilizing agents and storing the tissue graft in the solution for a period of time of at least 24 hours while the solution is in an unfrozen state. A tissue preservation system includes a solution containing one or more antimicrobial and / or stabilizing agents and a tissue graft in the solution.

The present application belongs to the technical field of tissue transportation, and particularly relates to a tissue preservation and transportation device and a tissue preservation reagent, which aims to solve the technical problem that the activity of living tissue is reduced or the living tissue is dead after transportation in the prior art, and it is difficult to retain the original tissue characteristics. The tissue preservation and transportation device comprises a tissue preservation reagent and a preservation tube. The tissue preservation reagent comprises a temperature-sensitive gel, an antioxidant stress component and a tissue preservation solution. The preservation tube is used for containing the tissue preservation reagent. The tissue preservation and transportation device and the tissue preservation reagent utilize the tissue tube and the tissue preservation reagent to effectively buffer the jolt suffered by the tissue during transportation. The characteristics of the temperature-sensitive gel facilitate the transfer of the tissue block and the removal of the tissue block. The antioxidant stress component can keep the tissue characteristics unchanged. The tissue preservation solution can keep the tissue cell activity, and plays a good protection role on the tissue during transportation. The tissue preservation and transportation device provides a simple and effective method for tissue preservation and transportation.

The invention belongs to the field of biology, and discloses a tissue formula preserving fluid containing antimycin A. The tissue formula preserving fluid comprises a basic culture medium, the basal culture medium contains superoxide dismutase, catalase and antimycin A. On the basis of the superoxide dismutase and the catalase, the preservation solution is matched with the antimycin A, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, when the respiratory chain inhibitor drug is applied to the formula of the invention, the improvement degree of the cell viability of only antimycin A is the highest; 2, the function of antimycin A must be exerted based on the use of superoxide dismutase and catalase; meanwhile, the invention further provides a tissue preservation method.

The invention relates to the technical field of medical instruments, in particular to a tissue preservation device for pathology analysis of the digestive system department. A cabinet body is arranged on a moving trolley, a cylindrical groove is formed in the middle of a disc-shaped base with the bottom end connected to a top plate of the cabinet body in a sleeving mode, and a plurality of cylindrical cavities are formed in the outer edge of the disc-shaped base; a central hole and a through hole are formed in the disc-shaped shell sleeved at the top end of the disc-shaped seat; the sample cabin is sleeved in the cylindrical cavity; the liquid supplementing tank and the sterile gas tank are arranged in the cabinet body; a gas path input end and a liquid path input end of the gas-liquid distribution part are respectively connected to output ends of the sterile gas tank and the liquid supplementing tank, the liquid nitrogen assembly comprises a tank seat fixed on the bottom surface of the cylindrical groove, a liquid nitrogen tank and a one-way valve arranged at the bottom of the liquid nitrogen tank, and the tank seat is communicated with an inner cavity of the sample cabin; a sealing plate is arranged in a through hole in the outer edge of the disc-shaped shell rotationally sleeved in the disc-shaped shell; the driving part is used for driving the turntable to rotate; a gas-liquid input piece is arranged at the bottom of the sample container, and stationary liquid adopts a new formula. Pathological analysis tissue samples collected by the digestive system department can be safely and effectively stored.

The herein disclosed invention relates to a method of preparing a tissue sample for microscopic analysis comprising an iterative expansion scheme. The method comprises use of three different independent and non-crosslinked hydrogels. Moreover, the invention relates to hydrogels prepared according to the disclosed methods and their use in light microscopy.

The invention provides a tissue phase homogenizing agent and a preparation method and application thereof, and belongs to the technical field of biological tissue engineering, the homogenizing agent is composed of hydrophilic matrixes (glucose, polyglycerol, poloxamer F127 and polyvinyl alcohol), functional additives (mannose, N-acetylcysteine, sodium hyaluronate and polyethylene glycol) and ultrapure water, and the pH value is 7.0 + / -0.2. Hydrophilic groups and water molecules form a hydrogen bond network, a continuous hydration layer is constructed on a phase interface, phase domain fusion, refractive index homogenization and molecular ordering are achieved, the problem of biological tissue phase homogenization is solved, tissue light transmittance can be improved, the swelling ratio can be stabilized, and the hydrogel has cell proliferation promoting activity and is suitable for tissue transplantation operation and bioengineering tissue preservation.

The invention belongs to the technical field of tissue preservation and cell culture, and particularly relates to a nasal mucosa tissue cryopreservation solution, a nasal mucosa tissue preservation kit and application of the nasal mucosa tissue cryopreservation solution in nasal mucosa tissue preservation. The nasal mucosa tissue cryopreservation liquid can be used for safely and efficiently storing the nasal mucosa tissue. The nasal mucosa tissue preservation kit comprises the nasal mucosa tissue cryopreservation liquid and the nasal mucosa tissue resuscitation liquid, and after the nasal mucosa tissue cryopreservation liquid is used for conducting cryopreservation on nasal mucosa tissue, the nasal mucosa tissue resuscitation liquid can conduct safe resuscitation on the cryopreserved nasal mucosa tissue; the stem cells obtained by culturing the resuscitated nasal mucosa tissue and the stem cells obtained by culturing the fresh nasal mucosa tissue have no obvious difference in form, cell markers and purity. And meanwhile, the pain of the collected person caused by collecting the tissue blocks for multiple times is avoided.

The invention discloses a prostate cancer organoid culture medium and a culture method thereof, and particularly relates to the technical field of prostate cancer organoid culture, and the prostate cancer organoid culture medium comprises a basic culture medium and the following components: 20-100ng / ml of epidermal growth factors, 20-100ng / ml of prostate cancer organoid and 20-100ng / ml of The concentration of Noggin is 50 to 500 ng / ml; the concentration of R-spondin is 50 to 500 ng / ml; the weight of Wnt3a is 50 to 500 ng / ml; the concentration of FGF is 101 to 50ng / ml; 1 to 50 ng / ml of FGF (fibroblast growth factor); stem cell factors are 1 to 50 ng / mL; the concentration of antibiotics is 50-500 ng / ml; dHT is 1 to 50 ng / ml; the method comprises the following steps: 1, obtaining a prostate cancer tissue sample, placing the prostate cancer tissue in a sampling bottle containing a tissue preservation solution at 2-8 DEG C, and rapidly transferring the prostate cancer tissue to a clean laboratory for subsequent treatment; 2, preparing a plurality of culture dishes, adding a 4 DEG C precooled prostate cancer organoid culture buffer solution for later use, disinfecting a sampling bottle, putting the tissue into the culture dishes, and cleaning the tissue for three times by using the prostate cancer organoid culture buffer solution; the culture medium and the culture method provided by the invention can promote the growth of organs with prostatic cancer, so that the culture medium has a better actual use effect and is convenient to popularize and use.

The invention provides a tissue preservation solution for autologous tooth transplantation and a preparation method and application thereof, and relates to the technical field of biology, and the tissue preservation solution comprises a high-glucose DMEM culture solution, I TS (insulin-transferrin-selenium), dexamethasone, L-ascorbic acid-2-phosphoric acid and penicillin / streptomycin. The method can provide enough metabolic substrates, improve energy supply of cells, ensure that high activity of periodontal ligament cells is kept within the preservation time of more than 6 hours, and is suitable for long-time transportation and complex clinical operation environments, so that the success rate and clinical effect of autologous tooth transplantation are greatly improved, and the method is suitable for popularization and application. In addition, not only is the cell viability protection capability improved, but also the anti-inflammatory and repairing capabilities are enhanced.

Disclosed herein include methods, compositions, and kits suitable for use in preservation (e.g., cryopreservation) of samples. The composition (e.g., cryopreservation reagent) can comprise: one or more cryoprotective agent(s), one or more culture media component(s), one or more carbohydrate(s), and / or one or more amino acid(s). The composition can be capable of maintaining one or more cellular properties and / or cell viability of a plurality of cells in a sample for a period of time under a storage condition. Also provided are methods of sample preservation and sample analysis. The method can comprise contacting a sample with the cryopreservation reagent, thereby generating a shielded sample. The method can comprise exposing the shielded sample to a freezing storage condition, thereby generating a cryopreserved sample.

A powder formulation for preserving tissue and organ function, and more particularly to shelf stable powder formulations that can be reconstituted at point of use, including during a fat grafting process, coronary artery bypass graft surgery, and peripheral bypass surgery, or other grafting / transplant surgery prior to or during the medical procedure to preserve the tissues / organs used in these procedures. The powder formulation includes a reducing agent; an antioxidant; a sugar; a nitric oxide substrate; a buffering agent; and physically acceptable salt. Also provided are a method of preparing an organ or tissue preservation solution by reconstituting the powder formulation, and a method of preserving tissue or an organ using same.

The utility model discloses a perinatal tissue transportation suit which comprises a tissue storage bag, a liquid adding assembly is arranged at one end of the tissue storage bag, and a sealing assembly is arranged at the other end of the tissue storage bag. The liquid adding assembly comprises a spiral opening, a movable clamp and a hose, and the sealing assembly comprises a sealing clamp and a double-sealing strip. According to the utility model, the hose is arranged at one end of the tissue preservation bag, and the spiral opening and the movable clip are arranged on the hose, so that tissue preservation liquid can be firstly guided into the tissue preservation bag, and a transportation suit containing the preservation liquid is directly submitted to a collection person; the sealing clip is additionally arranged on one side of the double-sealing strip, the sealing effect is enhanced while sealing is convenient, the sealing clip is provided with scale marks, and whether the umbilical cord length meets the collection standard or not can be preliminarily judged; the easy-to-tear spout is additionally arranged on the tissue preservation bag, an operator in a laboratory can tear the easy-to-tear spout along a dotted line, liquid is poured into a waste liquid cylinder, and the phenomenon of liquid splashing caused when the tissue and the liquid are transferred together can be effectively avoided.

The present invention relates to a tissue preservation solution for aborted tissues, characterized in that the tissue preservation solution for aborted tissues comprises PBS and a tissue preservation solution; preferably, the tissue preservation solution for aborted tissues is a mixed solution of PBS and the tissue preservation solution. The present invention provides for the first time a tissue preservation solution that can stably preserve and transport aborted tissues at room temperature, reducing the temperature requirements during the preservation and transportation of aborted tissues, thereby achieving the purpose of saving preservation costs and reducing transportation costs. At the same time, through the research on the proportioning of the tissue preservation solution, the proportion of the expensive tissue preservation solution in the tissue preservation solution for aborted tissues of the present invention is reduced, and the cost of the tissue preservation solution for aborted tissues is lowered.

The invention belongs to the field of biology, and discloses a tissue sample preservation solution which comprises a basic culture medium, the basic culture medium contains fetal calf serum and MitoQ, and FBS and MitoQ are added and used on the basis of the basic culture medium, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, in the presence of FBS, only MitoQ in the mitochondrial targeting antioxidant can show a relatively strong tissue activity maintaining effect; 2, the effect of MitoQ in a basic culture medium is not fully played, and the MitoQ must be played in the presence of FBS; meanwhile, the invention further provides a tissue preservation method.

The invention relates to the technical field of tissue preservation solutions, and provides a solid tissue flow detection sample preservation solution and a preparation method thereof, and the preservation solution comprises N-acetylcysteine, melatonin, lactobionic acid and an HBSS buffer solution. NAC, melatonin and lactobionic acid jointly act on an antioxidant pathway, ROS is removed, accumulation of lipid peroxidation products (MDA) is reduced, the mitochondrial function is protected, and the cell survival rate is increased. The melatonin and lactobionic acid act together to inhibit ferroptosis paths, maintain the health state of cells and improve the cell survival rate and the stability of antigen epitopes. All the components and a stable environment provided by HBSS act together to maintain the high activity of cells and the stability of antigen epitopes.

The invention belongs to the technical field of tissue engineering, cell biology and tissue preservation, and particularly relates to a serum-free adherent tissue preservation method and application of the serum-free adherent tissue preservation method in primary cell separation and amplification. The serum-free adherent tissue preservation method comprises the following steps: cutting a to-be-preserved tissue into blocks, placing the blocks in a serum-free basal culture medium, and preserving the blocks under a controlled, simulated and close-to-physiology metabolic microenvironment condition for at least 14 days. According to the technical scheme, freezing and a high-concentration cryoprotectant are not needed, operation is easy and convenient, the heterogenous pollution risk is avoided, the tissue can be stably preserved for at least 14 days, cell emigration has high controllability, the method is suitable for various scientific research and clinical scenes, the problems that in the prior art, the tissue preservation period is short, cell damage is large, operation is complex and the like are solved, and wide application prospects are achieved.

The present invention provides methods and compositions for storage and preservation of tissues, for example peripheral nerve allografts, for extended periods of time at room temperature in a sterile tissue culture container or bag.

A tissue cutting device that is especially suited for neurosurgical applications is disclosed and described, as well as alternative systems for tissue preservation and transport. The cutting device includes an outer cannula in which a reciprocating inner cannula is disposed. A tissue collector is also provided and is in fluid communication with the lumen of the inner cannula. A temperature control sleeve may be disposed around the tissue collector to control the temperature of the tissue samples. A preservation system may be supplied that is configured to deliver fluids to tissue samples in the tissue collector. A fluid supply sleeve may be disposed about the outer cannula and is selectively positionable along the length of the outer cannula.

The invention discloses an external chest sampling tissue preservation device, and relates to the technical field of tissue preservation, the external chest sampling tissue preservation device comprises a box body, a storage box, a refrigerator, a partition plate, a butt joint cover, an air supply pipe, a sealing assembly and a butt joint pipe; a plurality of groups of butt joint pipes are arranged and correspond to the storage boxes one by one; each group of butt joint pipes comprises two butt joint pipes which are arranged left and right; a plurality of groups of butt joint covers are arranged and are in one-to-one correspondence with the butt joint pipes, and each group of butt joint covers comprises two butt joint covers which are arranged left and right, are fixed at the rear part of the inner wall of the storage box and are communicated with the butt joint pipes; the multiple sets of sealing assemblies correspond to the storage boxes one to one, are fixed to the partition plate and correspond to the storage boxes in position, each sealing assembly is provided with a horizontally-arranged blocking block, and the blocking blocks and the butt joint pipes are located on the same horizontal line and used for sealing the butt joint pipes through the magnetic attraction effect to prevent cold air leakage; the technical effects that the sealing performance is prevented from being reduced due to mechanical abrasion, the heat preservation effect is stable, and the application range is widened can be achieved.

The invention relates to a method for preservation of a tissue or an organ previously obtained from a mammal, for transplant of said tissue or organ in a recipient compatible mammal, the method comprising: - contacting, preferably in vitro or ex vivo, said tissue or organ with a treating composition, the treating composition comprising a neddylation pathway inhibitor, for 1 to 12h hours, in order to obtain a treated tissue or organ, said composition comprising from 1 to 100 µM of said neddylation pathway inhibitor; - washing the treated tissue or organ with a conservation composition for removing the treating composition from the treated tissue or organ, in order to obtain a washed tissue or organ; and - contacting washed tissue or organ with a preservation composition, in order to obtain a ready to be transplanted tissue or organ.

The utility model discloses an animal tissue storage device with shockproof structure relates to animal tissue storage technical field. The utility model discloses a arc bottom plate and fixed clamping plate, arc bottom plate top is provided with fixed frame, and the outer wall of fixed frame is provided with elastic link, and fixed clamping plate sets up at elastic link one end, and the outer wall of fixed clamping plate is provided with push slide, and the inside of fixed frame is provided with adjusting sliding slot, and the inside of adjusting sliding slot is provided with adjusting slide plate. The utility model discloses setting up the protection mechanism, not only can when test tube is placed to the inside of the placing groove, the test tube is fixed with clamping, can avoid because the road surface is uneven and drives test tube and the animal tissue sample in test tube to shake, makes animal assembly sample and test tube inner wall to collide and causes animal tissue deformation, rupture or cell damage to animal assembly sample, and can adjust the clamping strength of protection mechanism to test tube according to the clamping demand of test tube, can guarantee the stability of test tube in the transportation process.

The present invention has found that soluble P-selectin (sP-sel) can interfere with the interaction between stem cells and the niche and, thus, mobilize stem cells from the bone marrow. Accordingly, mobilizing stem cells with sP-sel can treat an individual in need of one or more of tissue preservation, repair, or regeneration or neovascularization in an individual.

The invention discloses a preserving fluid for preserving pancreatic cancer tissues and application of the preserving fluid. The preserving fluid is prepared from mannitol, reduced glutathione, allopurinol, magnesium sulfate, gabexate mesylate, orlistat, pyruvic acid, Primocin, dexamethasone, heparin, bovine serum albumin, HEPES and the balance of an advance DMEM / F12 culture medium. The preserving fluid has a good effect in maintaining the pancreatic cancer tissue activity and inhibiting self-digestion and oxidative damage, is beneficial to maintaining the cell activity, and is beneficial to the preservation of pancreatic ductal adenocarcinoma in-vitro tissues and the construction of subsequent organoid.

A live tissue storage solution is formed of an aqueous culture medium including additives, inorganic salts and nutrients. The additives preferably include amino acids including but not limited to L-Arg, L-Cys, Gly, L-His, L-Ile, L-Lys, L-Leu, L-Met, L-Phe, L-Ser, L-Try, L-Try, and L-Val. The inorganic salts preferably include calcium chloride, sodium chloride, magnesium sulphate, sodium bicarbonate, sodium phosphate. The nutrients preferably include glucose, choline, folic acid, nicinamide, riboflavin, which are known to maintain cell culture. Examples include Dulbecco's Modified Eagle Medium DMEM or Minimal Essential Medium MEM with or without serum. The culture medium contains additives to reduce, inhibit, or mitigate lipid oxidation, lipotoxicy, lipid-induced responses, and lipid inflammation.

A tissue storage solution includes hypothermic storage compositions and methods. The hypothermic storage composition includes media containing Dulbecco's modified Eagle's medium (DMEM) and albumin. A method of hypothermically storing tissue includes storing such tissue in a storage medium including DMEM and albumin. A method for wound or defect treatment includes applying tissue, stored in a hypothermic storage medium containing DMEM and albumin, to the site of such wound or defect.