100 results about "Tissue Preservation" patented technology

Compositions that include nanoscale structured materials or precursors thereof (e.g., self-assembling peptides) are described. The compositions can include other substances (e.g., a vasoconstrictor). Also described are methods for using the compositions to promote hemostasis, to protect the skin or wounds from contamination, to decontaminate a site upon removal of previously applied compositions that provided a protective coating, and to inhibit the movement of bodily substances other than blood. The compositions are also useful in isolating tissue, removing tissue, preserving tissue (for, e.g., subsequent transplantation or reattachment), and as bulking, stabilizing or hydrating agents. Medical devices that include the compositions (e.g., a stent or catheter), bandages or other wound dressings, sutures, and kits that include the compositions are also described.

The present invention provides for compositions and methods for the preservation of tissues and organs ex vivo and in situ. In addition, the present invention provides for kits that may be used in the preparation of the solutions of the present invention.

The present invention provides for compositions and methods for the preservation of tissues and organs ex vivo and in situ. In addition, the present invention provides for kits that may be used in the preparation of the solutions of the present invention. The present invention also provides a device for perfusing tissues and organs with the solutions of the present invention.

Compositions which self-assemble under physiological conditions are formulated for application to wounds. The formulations include a pharmaceutically acceptable carrier or are provided as part of a medical device or coating. The formulations may also include other therapeutic, prophylactic or diagnostic agents. The formulation can be administered as appropriate for treatment of one or more disorders or conditions. For example, the formulation may be applied to repair an injury or during surgery of the lung, eye or dura, or following an epidural or spinal tap, to stop leakage of blood, interstitial fluid, or cerebrospinal fluid. The formulation may be administered to a burn or ulcer. The formulation may be dispersed in a suture or adhesive for administration at the time of or as released following suturing or gluing of a wound, thereby limiting bleeding, loss of tissue fluids, or other fluids such as those produced by parenchymal tissues such as the liver, pancreas, and gastrointestinal tract. The formulation may be applied to any site of bleeding, in a bandage, gauze, sponge, or other material, for immediate control of bleeding, or released later to control bleeding if the initial treatment such as suturing or pressure is insufficient. In one embodiment, the formulation is provided as a dry or lyophilized powder. In another embodiment, the material is provided in water. In another embodiment, the material is provided in combination with an oil and forms a laminate. In another embodiment, the formulation is provided as a coating on a device, for example a stent or a catheter. The material is also useful to isolate tissue, for preservation of tissue for subsequent transplantation or reattachment, and as a bulking, stabilizing or hydrating agent.

Compositions that include nanoscale structured materials or precursors thereof (e.g., self-assembling peptides) are described. The compositions can include other substances (e.g., a vasoconstrictor). Also described are methods for using the compositions to promote hemostasis, to protect the skin or wounds from contamination, to decontaiminate a site upon removal of previously applied compositions that provided a protective coating, and to inhibit the movement of bodily substances other than blood. The compositions are also useful in isolating tissue, removing tissue, preserving tissue (for, e.g., subsequent transplantation or reattachment), and as bulking, stabilizing or hydrating agents. Medical devices that include the compositions (e.g., a stent or catheter), bandages or other wound dressings, sutures, and kits that include the compositions are also described.

An object of the invention is to provide a tissue preservation solution that has excellent tissue-preserving ability and is useful in the field of medicine, medical experiment, etc. Thus, the invention relates to a tissue preservation solution including oxygen nanobubbles.

This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that is effective to fix the phosphoproteins, and that has a sufficient water content to be soluble for a stabilizer and / or a permeability enhancing agent); (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; and (3) a permeability enhancing agent (e.g. PEG). Methods are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. the following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample.

Methods and compositions for resuscitating, storing, and preserving functional integrity of organs and tissues. Metabolic function is maintained by sustaining ATP levels, mitochondrial function, cardiomyocyte contractility, prevention of acidosis, inhibition of induction of apoptosis, maintaining ionontrophy and lusiotrophy by regulating calcium, sodium, potassium and chloride ions.

The present application relates to methods for increasing stability of formalin fixed, optionally paraffin embedded biological material. In one example, DNAse inhibitors and / or RNAse inhibitors are combined with the biological material or formalin at the time of fixation or shortly prior.

A sterilized device for storing and transferring a tissue sample collected in a sterile field is provided having a hermetically sealed package containing a tissue storage chamber sealed with a removable cap creating a sample storage chamber housing a tissue preservation agent. The package may be provided as a lid sealed to a tray, a plastic bag that is sealed, or two pieces of plastic sealed along the edges. An interior compartment is created within the package, which is sterile along with the sample container and its contents. A tissue preservation agent is provided to stabilized RNA in a tissue sample. The sterilized device is intended for use in storing and transferring a tissue sample from a patient while in a sterile field. The sterilized device is a single assembly that eliminates disrupting a sterile field in an operating room and transferring an exposed tissue sample out of the sterile field.

Methods and compositions for resuscitating, storing, and preserving functional integrity of organs and tissues. Metabolic function is maintained by sustaining ATP levels, mitochondrial function, cardiomyocyte contractility, prevention of acidosis, inhibition of induction of apoptosis, maintaining ionontrophy and lusiotrophy by regulating calcium, sodium, potassium and chloride ions.

The invention provides an antifreeze glycolipid compounds and composition comprising a polysaccharide moiety of Formula I:wherein D-Manp represents a D-mannopyranose moiety, D-Xylp represents a D-xylopyranose moiety, and n is about 5 to about 70; and one or more lipid moieties covalently linked to the polysaccharide moiety of Formula I or electrostatically associated with the polysaccharide moiety of Formula I. The antifreeze glycolipid compounds and compositions can be used for a variety of industrial, agricultural, medical, and cosmetic applications where recrystallization-inhibition, cyroprotection, or cryopreservation is desired. The antifreeze glycolipid compounds or compositions can be used as, for example, as cryoprotectants for tissue preservation and transplantation, improving the texture of processed frozen food and frozen meats, frostbite protection, crop protection, and green alternatives for land vehicle antifreeze and aircraft de-icing.

Hypothermic therapy headgear for patients includes a headpiece for engaging the head of the patient and a cooling member engaged to the headpiece for contacting at least one surface portion of the patient. The cooling member includes at least one cooling surface for contacting the surface portion of the patient, and at least two endothermic reaction components. The endothermic reaction components have an initial state where the endothermic reaction components are separated from contact with each other, and a treatment state in which the endothermic reaction components are placed into contact, wherein an endothermic reaction takes place and cools the cooling surface and the corresponding portion of the patient. An activation device selectively places the endothermic reaction components into the treatment state when a patient is in need of hypothermic therapy. A thermal therapy device and a method for administering thermal therapy are also disclosed.

A process of preserving tissue from one or more plant, fungus or algae species is provided. The process comprises treating such tissue by subsequent steps of (i) incubation with a weak acid solution; (ii) removal of excess acid; (iii) incubation with an aqueous sugar alcohol solution; (iv) removal of excess sugar alcohol; and (v) drying. Also provided is a textile material having a sheet like structure, and characterized in having an average thickness of about 0.2-5 mm and comprising about 2% to about 20% sugar alcohol.

A tissue sample preservation process and device for improving and standardizing tissue preservation procedure, including placing tissue specimens in a cold fixative, performing fixative penetration at a refrigerated temperature, and accelerating fixative penetration by ultrasound. Also disclosed is the application of ultrasound and temperature control in the dehydration, clearing, and impregnation steps.

The invention discloses in-vitro tissue preserving fluid and a preparation method thereof. Each 1,000 milliliters of preserving fluid comprises the following components by weight: 5 to 200 grams of osmotic pressure regulator, 0.2 to 40 grams of a pH value buffer pair, 3 to 15 grams of potassium chloride, 1 to 5 grams of sodium chloride, 10 to 300 milligrams of antibiotic, 5 to 100 grams of glucose and the balance of deionized water. In the invention, a non-CO2 dependent buffer pair is used to reduce the dependence on CO2 in transport and an air exposure process and reduce the fluctuations of the pH value; a hypertonic and high-potassium environment is used to reduce the swelling of cells and tissues and improve the activities of the cells and tissues; and the antibiotic used is free from causing hypersensitivity reaction. The preparation method is simple and in-vitro tissue preserving fluid has a remarkable in-vitro tissue preserving effect. The in-vitro tissue preserving fluid is low in cost, has low transport requirements, and can be stored at room temperature and conveniently.

The present invention relates to a composition for preserving cells, tissues and organs, comprising as an active ingredient indole and indazole compounds of formula (1), or a pharmaceutically acceptable salt or isomer thereof, which are effective for preventing injury of organs, isolated cell systems or tissues caused by cold storage, transplant operation or post-transplantation reperfusion; a preservation method; and a preparation method of the composition.

The invention relates to the field of organoid culture, in particular to a culture medium, a method and a kit for rapidly culturing tumor organoids. According to the culture medium for culturing the tumor organoids provided by the invention, the time for culturing the tumor organoids can be greatly shortened. The kit for rapidly culturing the tumor organoid comprises a culture medium for culturing the tumor organoid, a tissue preservation solution, a tissue digestion solution and a cell mass collection solution, and when the kit is used for culturing the tumor organoid, the success rate and survival rate of culturing the tumor organoid can be increased, an organoid model can be rapidly established within 2-5 days, which can be applied to subsequent experimental tests.

The invention discloses a placenta tissue matrix material. A placenta extracellular matrix is prepared by taking placenta tissue as a raw material through the technologies of tissue preservation, tissue treatment, sterilization, virus inactivation and the like, the placenta extracellular matrix retains a natural matrix structure and basic biological characteristics, and the extracellular matrix particularly retains receptors of various active factors. A preparation method comprises the following steps: tissue pretreatment; sterilization and virus inactivation; tissue DNA removal; decellularization; and preparation of various forms such as gel, sponge or powder from the acellular matrix according to specific clinical requirements. The acellular matrix disclosed by the invention is a specialscaffold material with an active factor receptor, can actively attract active ingredients in a living body into the material, is beneficial to accumulation of cell active ingredients, supports cell migration, adhesion, proliferation and differentiation, and can accelerate damaged tissue repair and local tissue reconstruction.

A tissue sample preservation process and device for improving and standardizing tissue preservation procedure, including placing tissue specimens in a cold fixative, performing fixative penetration at a refrigerated temperature, and accelerating fixative penetration by ultrasound Also disclosed is the application of ultrasound and temperature control in the dehydration, clearing, and impregnation steps.

The invention belongs to the field of biotechnologies and relates to a method for preserving rubber tree leaf tissues extracted by DNA (deoxyribonucleic acid). The method comprises the following steps: placing collected rubber tree leaves in a pre-cooled mortar, and utilizing liquid nitrogen for grinding until the leaves become powder; and then transferring the powdery leaf tissues into a centrifugal tube containing storage buffer solution, uniformly mixing and placing in a low-temperature environment for preservation. The method which combines the storage buffer solution with low-temperaturerefrigeration is utilized for preserving the rubber tree leaf tissues, thereby effectively realizing the freshness of the rubber tree leaves for a long time, facilitating long-distance sampling, material transportation and storage, and having the characteristics of simplicity in process, low cost, good effects and the like.

The invention belongs to the technical field of detection equipment in the pathology department, and particularly to a slicing device for sample manufacturing in the pathology department. Specific tothe problems that the slicing device for sample manufacturing in the pathology department cannot be used for continuous slicing, slicing accuracy is low, consumed time is long and preservation is improper after slicing, the invention provides a scheme as follows: the slicing device comprises a mounting rack, supports are welded with the tops of outer walls on two sides of the mounting rack, a workbench is fixed on the outer walls of the tops of the supports through screws, and a sample manufacturing box is fixed at the top of an outer wall on one side of the workbench through screws. By the sample manufacturing box, subsequent samples are convenient to distribute uniformly, a magnetic head and a magnetic slicing cutter head cooperate with glass slides, paraffin blocks can be simultaneouslycut into multiple groups of slices with uniform thicknesses, the need for tissue to be sliced step by step is avoided, meanwhile, time consumed by tissue cutting is shortened, a sample slice storagebox can keep the optimum temperature for tissue preservation, and the dyeing problem caused by non-uniform slicing due to tissue unfreezing and softening is prevented.

An antifreeze glycoprotein comprising at least one C-linked saccharide according to the following general formula:wherein X is nitrogen, sulfur, carbon or (CR1R2)nS═O wherein n is 0, 1, 2 or 3, Y is carbon, nitrogen, sulfur or oxygen, R1 is hydrogen, methyl, fluorine, a carbohydrate, or a hydroxy chain, R2 is hydrogen, methyl, fluorine, a carbohydrate or a hydroxy chain, R3 to R6 independently represent an alkyl chain, a hydroxy group, a dimethyl sulfoxy group, a carbohydrate or an ether, R7 is ornithine, lysine, an alkyl chain, a pyridyl group or a heterocycle; and R8 is hydrogen, fluorine a carbohydrate or a hydroxy or methoxy group. Such antifreeze glycoprotein analogues are useful recrystallization inhibitors and may be used as a cryoprotectant for tissue preservation and transplantation, improving the texture of processed frozen food and frozen meats, frostbite protection, crop protection, and green alternatives for land vehicle antifreeze and aircraft de-icing.

The invention relates to the field of tissue engineering technology, and in particular to a breast repair patch and a preparation method thereof. Problems in the prior that collagen bundles on the surface of a loose layer become free, floating and even falling off are solved; appearance quality of the finished product (the breast repair patch) is improved and service cycle of tissue preservation liquid and preservation cycle of the finished product are prolonged; and meanwhile, the breast repair patch, when used, can facilitate adhesion and fixation of the membrane tissue loose layer, accelerate in-growth and migration of breast cells and tissues, induce generation of new tissues and greatly relieve such symptoms as hard breasts, pain and the like after an operation. According to the embodiment, the breast repair patch provided by the invention comprises a mammal membrane tissue which comprises a dense layer and the loose layer and a collagen coating which is formed on the surface of the loose layer.

The invention belongs to medical anatomy, and particularly relates to a novel cadaver specimen preserving fluid. The novel cadaver specimen preserving fluid comprises the following components in parts by mass: 0.6-6 parts of phenoxyethanol, 0.6-6 parts of potassium sorbate, 0.3-1 part of vitamin C, 0.1-0.6 part of musk oxalic acid, 0.6-1.5 parts of sodium chloride, 0.6-1.5 parts of ferric chloride and 78-97 parts of water. The novel cadaver specimen preserving fluid is good in preservation effect, and reliable in antiseptic and anti-mold properties on the cadaver specimen; the specimen does not mould, does not shrink, is natural in color, and flexible and moist in tissue; and the preserving fluid is non-toxic, free of a bad pungent smell, free of sediment, and free of an effect on health of a worker, and does not need to be exchanged in the use procedure. The novel cadaver specimen preserving fluid is relatively ideal cadaver specimen preserving fluid in education and research at present, and is good substitute goods of formalin in long-term tissue preservation.

The invention discloses an in-vitro tissue preserving fluid as well as a preserving method and application thereof. The in-vitro tissue preserving fluid includes a penicillin-streptomycin solution, fetal calf serum and an improved L-15 culture medium. The obtained in-vitro tissue preserving fluid can provide nutrition for tumor tissues and has an antibacterial effect, the activity of the tumor tissues can be improved, the modeling success rate of clinical patient-derived tumor xenograft is further improved, in addition, tumor tissue samples can be preserved, a sample database can be established, secondary experiment needs are facilitated, and repeated material taking is reduced.