15 results about "Collagen fiber bundle" patented technology

To provide a rotating frame apparatus and biocompatible scaffold construct.SOLUTION: A biocompatible scaffold construct includes a plurality of collagen fiber strands, a first portion of which have been coated by a first biocompatible solution and, optionally, a second portion of which have been coated by a second biocompatible solution different than the first biocompatible solution. The coatings may include cells. Furthermore, the scaffold is constructed on rotating frame collectors.SELECTED DRAWING: Figure 7

The present invention provides a soft tissue composite. The soft tissue composite comprises a first collagen fiber bundle of a first soft tissue isolated from a first donor and a polymeric material, and the first collagen fiber bundle interconnects with the polymeric material. The soft tissue may be a sandwich composite comprising a first layer comprising a first collagen fiber bundler, a second layer comprising a polymeric material, and a third layer comprising a third collagen fiber bundles, and the second layer is between the first and third layers, and the first collagen fiber bundler, the polymeric material and the third collagen fiber bundler interconnect with each other. Also provided is a method of repairing a defective tissue in a subject with the soft tissue composite and a method of preparing the soft tissue composite.

The invention relates to an energy-absorbing bionic lattice structure design method, which belongs to the technical field of bionics, and is characterized in that a bionic tendon wave lattice structure is designed by referring to an ostrich metatarsophalangeal joint tendon collagenous fiber bundle microstructure in nature; the energy absorption characteristic of the structure is analyzed through tests and simulation, and optimal structure parameters are obtained in combination with a genetic algorithm; the superelastic material and the bionic tendon wave lattice structure are selected to be combined, the diversified requirements of different products for mechanical properties can be effectively met, and in multiple application occasions, impact energy can be efficiently dispersed and absorbed, movement impact force can be effectively dispersed, and a high-quality damping effect can be provided.

A process for producing a multifilament collagen fiber bundle involves treating a multifilament support polymer-collagen fiber bundle having a plurality of monofilaments of collagen supported on a support polymer with a series of liquid media having a decreasing concentration of the support polymer to remove the support polymer from the multifilament support polymer-collagen fiber bundle. The support polymer is more soluble than the collagen in the liquid media and the gradual removal of the support polymer provides enough time for collagen molecules to self-assemble into fibrils. The resulting multifilament collagen fiber bundle has a D-band structure similar to natural collagen but shifted by 1 / 6th of the D-band. The multifilament collagen fiber bundle can be crosslinked with ultraviolet light in the absence of photo-initiator to provide a crosslinked collagen product with superior tensile strength and Young's modulus making the product useful in collagen-based biomaterials for tissue repair and regeneration.

This invention discloses a method for preparing acellular dermal matrix, belonging to the field of tissue engineering technology. The preparation method includes: selecting pelts from traceable animals aged one to three months, purifying them through steps such as soaking, fleshing, defatting, hair removal, liming, deliming, and softening in a tanning process to obtain acellular dermal matrix. The acellular dermal matrix obtained by this invention removes antigenic components such as cells, hair follicles, and extracellular proteins during the preparation process, minimizing its immunogenicity while preserving the collagen fiber structure in its extracellular matrix. Simultaneously, the preparation method of this invention moderately loosens the collagen fiber bundles. This process not only effectively alleviates the entanglement between fiber bundles but also successfully increases the exposure of hydrophilic groups, giving the acellular dermal matrix excellent hydrophilicity, which is more conducive to cell proliferation and migration.

The present invention discloses a degradable tissue engineering labrum scaffold and a preparation method thereof. The scaffold uses a degradable polymer and an inorganic component as raw materials. Based on the melt electrostatic spinning process, the molten raw materials are stretched into fibers under the stretching effect of the electric field and the airflow field. The fibers are attached to a receiving roller to obtain highly oriented fiber bundles or fiber membranes. The fiber bundles or fiber membranes are loaded with polylactic-co-glycolic acid (PLGA) microspheres containing the small molecule drug bergenin and transforming growth factor β1 by spraying or soaking. The oriented fiber bundles or oriented fiber membranes after loading are curled to obtain oriented fiber aggregates. The obtained oriented fiber aggregates are placed in a pre-made mold for shaping, and the labrum scaffold is obtained after a period of time. The labrum scaffold prepared by the present invention has the advantages of bionic structure, solvent-free, high strength and good biocompatibility. It imitates the basic structure of the natural labrum composed of highly oriented circumferentially running collagen fiber bundles and has high tensile strength.

The invention provides a periodontal disease tooth root regeneration treatment method and a multi-modal implant system, and relates to the technical field of stomatology and tissue engineering, and the method comprises the following steps: S1, minimally invasive extraction of autologous hDPSCs, CD146 + sorting, co-culture of the autologous hDPSCs, a gradient scaffold and growth factor-loaded microspheres in a dynamic culture device to form an ECM membrane-scaffold complex; s2, minimally invasive implantation of the complex under the guidance of a navigation guide plate, wherein the initial torsion resistance of the magnesium alloy retention nail is larger than or equal to 25 N.cm; s3, zirconium oxide all-ceramic crown repair is carried out 6 months after operation, an adhesive contains 20wt% of nano ZrO, dynamic culture parameters are as follows: 1Hz compressive strain, a double-channel perfusion flow rate ratio is (1: 2)-(1: 4), a culture medium contains 25-50ng / mL of BMP-2 and 5-8ng / mL of USAG-1, and the gradient scaffold has a three-layer pore structure: the porosity of an alveolar bone contact layer is 85 + / -3%. According to the system, a functional periodontal membrane is formed, and collagen fiber bundles are vertically inserted into cementum / bone tissue; the vascularization time is shortened by 50%, and the CD31 + vascular density reaches 28.3 + / -4.7 pieces / mm < 2 > in 14 days after operation.

The preparation method comprises the following steps: by taking a degradable polymer and an inorganic component as raw materials, stretching the raw materials in a molten state into fibers under the drafting action of an electric field and an airflow field on the basis of a melt electrostatic spinning process, and adhering the fibers to a receiving roller, so as to obtain a highly oriented fiber bundle or fiber membrane. A fiber bundle or a fiber membrane is loaded with polylactic acid-glycolic acid copolymer (PLGA) microspheres containing a small molecule drug bergenin and a transforming growth factor beta1 through spraying or soaking, and the loaded oriented fiber bundle or oriented fiber membrane is curled to obtain an oriented fiber aggregate; the obtained oriented fiber aggregate is put into a pre-manufactured mold to be shaped, and the labial glenoid stent is obtained after a period of time. The glenoid lip stent prepared by the invention has the advantages of bionic structure, no solvent, high strength, good biocompatibility and the like, simulates the basic structure of a natural glenoid lip consisting of highly-oriented collagen fiber bundles running in the circumferential direction, and has high tensile strength.

The application provides a functionalized repair stent and a preparation method and application thereof. The functionalized repair stent comprises a tubular stent, a fiber membrane covering the tubular stent and physiologically active molecules specifically combined with the surface of the fiber membrane; or the functionalized repair stent comprises a collagen fiber bundle, a fiber membrane covering the collagen fiber bundle and physiologically active molecules specifically combined with the surface of the fiber bundle and the fiber membrane; or the functionalized repair stent comprises a collagen fiber bundle, physiologically active molecules specifically combined with the surface of the collagen fiber bundle and a tubular stent covering the collagen fiber bundle; the tubular stent comprises a tubular structure and a coating covering the tubular structure. In the application, the functionalized repair stent with certain strength and slow-release effect is prepared by designing the structure of the functionalized repair stent, the functionalized repair stent can be prepared semi-mechanically and automatically, the efficiency is high, the functionalized repair stent can be degraded in the body, a secondary operation is not needed and the size and shape are controllable.

An optical method for determining collagen bundle orientation in bovine pericardium includes the use of a system having a light source which transmits light through a first polarizer, a tissue for making a prosthetic valve leaflet, and a second polarizer, where the light then illuminates a detector plate. The light that illuminates the detector plate is used to determine the orientation of collagen fiber bundles. The orientation of the collagen fiber bundles is used to determine where to cut the leaflet edges.

The invention belongs to the field of leather animal skin unhairing, and discloses a leather-making salt-enzyme mixed solution as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) adding neutral protease, alpha-amylase, peptidase, protease and glucanase into water to prepare a mixed enzyme solution; and (2) adding a salt substance into the mixed enzyme solution prepared in the step (1), and uniformly mixing to prepare the tanning salt-enzyme mixed solution. The tanning salt-enzyme mixed solution is applied to animal skin unhairing, collagen fiber bundle splitting and / or softening, the efficiency and effect of enzyme unhairing are improved, hydrolysis of skin grain surface collagen is reduced, and grain surface looseness is reduced; non-collagen components such as proteoglycan, elastic fiber and impure protein in the skin tissue are thoroughly hydrolyzed, so that the dispersity of collagen fiber bundles is improved.

The present invention addresses the problem of providing a method for evaluating a sagging / wrinkle state on the basis of a novel mechanism. The present inventors have found that frame-like collagen formed by collagen bundles is present in the dermal layer, and discovered that the frame-like collagen present in the dermal layer maintains skin shape and contributes to anti-sagging and anti-wrinkle of the skin. Therefore, the present disclosure relates to a method for evaluating a sagging / wrinkle state of skin by evaluating the frame-shaped collagen formed by collagen bundles in a target skin.

A method of producing a multifilament collagen fiber bundle involves treating a multifilament support polymer-collagen fiber bundle containing a plurality of collagen monofilaments supported on a support polymer to remove the support polymer from the multifilament support polymer-collagen fiber bundle using a series of liquid media containing a decreasing concentration of the support polymer. The support polymer is more soluble than collagen in a liquid medium, and the gradual removal of the support polymer provides sufficient time for collagen molecules to self-assemble to form fibrils. The resulting multifilament collagen fiber bundle has a D-band structure similar to native collagen, but is offset by 1 / 6 D-band. The multifilament collagen fiber bundle can be cross-linked through ultraviolet rays under the condition of no photoinitiator to form a cross-linked collagen product with excellent tensile strength and Young modulus, and the product is suitable for collagen-based biological materials in the field of tissue repair and regeneration.

The purpose of this disclosure is to find a novel function of an ergothioneine compound, and to provide a preparation using this function.　One embodiment of this disclosure is a composition for suppressing a decrease in the thickness of a collagen fiber bundle due to UVA irradiation, the composition containing an ergothioneine compound as an active ingredient.

The invention discloses a preparation method of an acellular dermal matrix, and belongs to the technical field of tissue engineering. The preparation method comprises the following steps: selecting traceable animal fur of one to three months old, and purifying through the steps of soaking, fleshing, degreasing, unhairing, liming, deliming, softening and the like in a tanning method to obtain the acellular dermal matrix. In the preparation process of the obtained acellular dermal matrix, antigen components such as cells, hair follicles and impure proteins are removed, the immunogenicity of the acellular dermal matrix is reduced to the maximum extent, and a collagenous fiber structure in an extracellular matrix of the acellular dermal matrix is reserved. Meanwhile, according to the preparation method, collagen fiber bundles are moderately released, and the process not only effectively relieves entanglement among the fiber bundles, but also successfully increases the exposure degree of hydrophilic groups, so that the acellular dermal matrix has excellent hydrophilicity and is more beneficial to proliferation and migration of cells.