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Bone tissue regeneration guiding membrane and preparation method thereof

A technology for bone tissue regeneration and guiding membrane, applied in medical science, surgery, coating, etc., can solve the problems of poor mechanical properties and water resistance of guiding membrane, mismatch of bone tissue growth rate, and difficulty in large-scale production. To achieve the effect of favorable wound healing, good biocompatibility and long retention time

Active Publication Date: 2014-01-29
SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties and water resistance of the guide membrane made of natural polymers are poor, and the degradation is too fast, which does not match the growth rate of bone tissue.
Chinese patent application 200410071932.1 discloses a double-layer collagen-based guided tissue regeneration material and its preparation method. In order to overcome the defect that the collagen material degrades too quickly, it uses a chemical cross-linking agent to improve the degradation resistance of the material and prolong the regenerative membrane in vivo. Degradation time; due to the residue of chemical cross-linking agents such as glutaraldehyde in the product will cause the rejection of the product by the patient's body, and its preparation method is complicated and requires high equipment conditions, which increases the cost and makes large-scale production difficult.

Method used

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  • Bone tissue regeneration guiding membrane and preparation method thereof
  • Bone tissue regeneration guiding membrane and preparation method thereof
  • Bone tissue regeneration guiding membrane and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Step 1. Preparation of antigen-removed membrane tissue

[0035] 1) Raw material pretreatment: take pig pericardium, remove surface blood stains and adipose tissue, shake and wash with normal saline for 3 times, each time for 15 minutes;

[0036] 2), degreasing treatment: place the cleaned membrane tissue in 0.1% (M / V) NaCl solution, shake it for half an hour, then place it in ether (analytical grade) solvent for ultrasonic treatment for 1 hour, and use 2% ( M / V) NaCl solution flushing;

[0037] 3) Trypsinization of the dense layer: Tie the degreased membrane tissue into a bag with the dense side facing outward and the loose side facing inward, and inject PBS buffer into the bag; In the trypsin solution, shake for half an hour, and then add 0.5M CaCl 2 In the solution, shake and wash for 10 minutes, untie the bag and rinse with water to restore the membrane tissue from a closed bag to a membrane;

[0038] 4) NaOH treatment: put the trypsin-treated membrane tissue into...

Embodiment 2

[0046] Step 1. Preparation of antigen-removed membrane tissue

[0047] 1) Raw material pretreatment: take pig peritoneum, remove surface blood stains and adipose tissue, wash with normal saline for 3 times, each time for 15 minutes;

[0048] 2), degreasing treatment: place the cleaned membrane tissue in 0.1% (M / V) NaCl solution, shake it for half an hour, then place it in ether (analytical pure) solvent for ultrasonic treatment for 1 hour, and use 2% (M / V) NaCl solution flushing;

[0049] 3) Trypsin treatment of the dense layer: tie the degreased membrane tissue into a bag with the dense side facing outward and the loose side facing inward, and inject PBS solution into the bag; In the trypsin solution, shake for half an hour, then add 0.5M CaCl 2 In the solution, shake and wash for 10 minutes, untie the bag and rinse with water to restore the membrane tissue from a closed bag to a membrane;

[0050] 4) NaOH treatment: put the trypsin-treated membrane tissue into a NaOH solu...

Embodiment 3

[0058] Step 1. Preparation of antigen-removed membrane tissue

[0059] 1) Raw material pretreatment: take bovine pericardium, remove surface blood stains and adipose tissue, wash with normal saline for 3 times, each time for 15 minutes;

[0060] 2), degreasing treatment: place the cleaned membrane tissue in 0.1% (M / V) NaCl solution, shake it for half an hour, then place it in ether (analytical pure) solvent for ultrasonic treatment for 1 hour, and use 2% (M / V) NaCl solution flushing;

[0061] 3) Trypsin treatment of the dense layer: tie the degreased membrane tissue into a bag with the dense side facing outwards and the loose side facing inward, and inject PBS solution into the bag; In the trypsin solution, shake for half an hour, then add 0.5M CaCl 2 In the solution, shake and wash for 10 minutes, untie the bag and rinse with water to restore the membrane tissue from a closed bag to a membrane;

[0062] 4) NaOH treatment: put the trypsin-treated membrane tissue into a NaOH...

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Abstract

A bone tissue regeneration guiding membrane. The invention employs an antigen-extracted mammal tissue membrane, which has a double layer structure of a compact layer and a loosening layer. The compact layer is formed by tightly arranged collagen fibers, and an external side of the compact layer is composited with a collagen coating containing active polypeptides; the loosening layer is formed by interlaced collagen fibers and is composited with ossification active factors. The prepared guiding membrane can prevent soft tissue from evolving and promote new bone formation, has good biological compatibility, hydrophilism and bone induction capability, and can be completely degraded in body without generation of harmful substances. Besides, the guiding membrane has an effect of isolating cell evolving, a characteristic of slow degradation and a longer retaining time in body than a current guiding membrane prepared from natural high-molecular polymer, and can provide enough time and space for new bone growth. The method of the invention is simple, and raw materials have wide sources and low prices, so that the method is suitable for large-scale industrial production and beneficial to lowering medical costs and mitigating patient burdens.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a biofilm material capable of guiding bone tissue regeneration and a preparation method thereof. Background technique [0002] Membrane-guided tissue regeneration (guided tissue regeneration, GTR) technology has been widely used in the field of bone tissue repair. Its principle is to prevent soft tissue cells from entering the bone growth area through the physical barrier effect of the membrane material, and to prevent new bone growth in the bone defect area. Leave space and guide osteoblasts to generate new bone in the bone defect area. [0003] The membrane used in the early stage of GTR technology is not absorbable in the body. For example, the GTR membrane made of polytetrafluoroethylene must be taken out by a second operation when it is used, which brings great pain to the patient, and because it has no biological activity, it cannot be used for rela...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/00A61L31/10
Inventor 杨改叶
Owner SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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