36 results about "New Bone Formations" patented technology

The invention relates to the technical field of biomedical materials, in particular to a preparation process of novel composite bone cement with biological activity, which comprises the following steps: calcining calcium phosphate in a calcining furnace at 800-1000 DEG C for 3 hours, cooling to 20 DEG C, grinding the calcium phosphate by using a grinding machine until the diameter is less than 100 mu m, and drying to obtain the novel composite bone cement with biological activity. Calcium phosphate micro powder is obtained; by fusing the calcium phosphate, the nano-hydroxyapatite, the bioglass and the collagen, the material has a mechanical support function of an inorganic phase and biological activity of an organic phase, the nano-hydroxyapatite is extremely similar to human bone minerals and can promote osteoblast attachment and growth, and the collagen constructs a bionic microenvironment and accelerates new bone formation, so that the material can be used for treating osteoporosis. The bone repair efficiency is obviously improved.

The application discloses a jaw bone defect negative pressure repair body with a flushing function and a preserved periosteum, which comprises a base body, the base body is a titanium alloy net structure formed by 3D printing, two ends of the base body are adapted to be attached to bone broken ends, and an outer surface of the base body is adapted to be contacted with jaw bone periosteum, the base body is internally provided with a negative pressure suction pipeline penetrating through the base body, an outer pipe opening of the negative pressure suction pipeline is connectable to a negative pressure suction device, a pipe wall of the negative pressure suction pipeline is distributed with through holes formed by the titanium alloy net structure, and a reserved flushing pipeline is arranged around an outer periphery of the negative pressure suction pipeline, and the reserved flushing pipeline is distributed with flushing holes around the outer periphery. The repair body has the following functions: promoting growth and differentiation of osteoblasts on the surface of the bone broken ends and the periosteum, and promoting new bone formation; and being capable of discharging tissue exudates and the like out of the body.

The present invention provides a pharmaceutical formulation, a pharmaceutical composition, a method for inducing new bone formation and a method for accelerating and enhancing spinal fusion and treating bone defects in a subject. The composition comprises autologous coagulum as the carrier for osteogenic bone morphogenetic protein BMP6, which has no immunogenicity to cause inflammation and ectopic bone formation and presents a higher potency for bone formation and reduction of drug usage.

An implant device configured to be at least partially in contact with bone on implantation has an improved osteoinductive feature to enhance new bone formation. The implant device has one or more bone growth surfaces extending from a structurally solid feature of the implant device. The one or more bone growth surfaces are configured to mimic adult trabecular bone by having trenches, grooves or surface recesses or prominences exhibiting numerous structural elements or walls not perpendicular to the surface that are non-coplanar or arched extending 20 to 500 microns in depth having an increasing inclination from the surface extending inwardly and not parallel to opposing or adjacent walls forming a random or non-random network. The one or more bone growth surfaces configured to mimic trabecular bone have discernable nano features on the structural elements or walls exhibiting nano scale features of less than 200 nano meters within the network.

The invention discloses an alpha-ketoglutaric acid functionalized hydrogel and application thereof in maxillofacial bone defect repair, and belongs to the technical field of biomedical materials. The hydrogel is constructed by crosslinking four-arm polyethylene glycol-sulfydryl and alpha-ketoglutaric acid through a Fe < 3 + > coordination bond. The hydrogel prepared by the invention has good mechanical properties, self-healing ability and inflammatory microenvironment responsive drug release characteristics, and can synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells, induce M2 polarization of macrophages and remove active oxygen, thereby optimizing a bone regeneration microenvironment. In-vivo experiments prove that the hydrogel can significantly promote the formation of new bones of a mouse jaw defect model. The invention provides a novel and efficient material solution for regeneration and repair of maxillofacial bone defects.

The invention belongs to the technical field of biomedical materials, and discloses a bone tissue repair material for promoting vascularization and new bone generation and a preparation method thereof. The bone tissue repair material is composed of a decalcified bone matrix and a degradable biological source gel material. The repair material has a loose porous three-dimensional structure similar to that of natural cancellous bone, and the temperature-sensitive gel material filled in the pore structure of the repair material can form gel in situ in vivo and is attached to pores of a decalcified bone matrix, so that cell migration, adhesion and proliferation can be promoted, the degradation rate of gel in vivo can be reduced, and the repair material has a good application prospect. The continuous release of the pro-angiogenesis factor is ensured. Good biocompatibility is achieved, and cytotoxicity and immunogenicity are avoided; meanwhile, in the slow degradation process, angiogenesis promoting factors are continuously released for a long time, and angiogenesis and new bone generation are promoted. Therefore, the implanted biological scaffold prepared from the bone tissue repair material has excellent bone repair and regeneration promotion capability and wide application prospect.

The unilateral alveolar split newborn maxilla orthopedic device comprises a palate plate and a retention stabilizing accessory, buccal side stretching accessories are connected to the outer portions of the left side edge and the right side edge of the palate plate, the material of the buccal side stretching accessories is softer than that of the palate plate, and the buccal side stretching accessories and the palate plate are of an integrated structure; the cheek side stretching accessory upwards protrudes out of the outer parts of the left and right side edges of the palate plate. The cheek side distraction accessory can generate an outward distraction effect on gingival tissues at the maxillary vestibular sulcus part, promote formation of new bones at the cheek side of maxillary alveolar bones, realize accurate quantitative controllable movement of alveolar bone sections, particularly effectively increase the transverse width, improve the comfort of the distraction effect due to the design of the edge buffer soft lining, and improve the safety of the distraction of the maxillary alveolar bones. The treatment effect is improved.

The present application relates to the technical field of bioactive glass, in particular to a preparation method and application of strontium-magnesium-doped bioactive glass, on the basis of selecting suitable raw materials, the co-precipitation method is adopted, strontium element is doped, and magnesium oxide is compoundedly added, so that the prepared bioactive glass has regular properties, good dispersible spherical particles, faster and stronger hydroxyapatite formation capacity, faster ion release rate, and can release calcium, phosphorus and silicon ions in simulated body fluid, form more hydroxyapatite, promote the proliferation and stability of bone cells, and stimulate new bone formation. The strontium-magnesium-doped bioactive glass is used in cooperation with mineral trioxide aggregate (MTA), so that cell adhesion, expression of bioactive enzymes, activity of fibroblasts can be promoted, and the strontium-magnesium-doped bioactive glass can be applied to the repair of human bone and dental pulp tissue.

The invention provides a method for inducing ankylosing spondylitis synovial fibroblast osteogenesis in vitro, and belongs to the technical field of cell culture, the ankylosing spondylitis hip joint synovial fibroblast is induced in vitro by using 100ng / mL of tumor necrosis factor-alpha (TNF-alpha) and 100ng / mL of bone morphogenetic protein 2 (BMP-2), and the ankylosing spondylitis hip joint synovial fibroblast can be converted into osteoblasts 12 days after induction. The ankylosing spondylitis synovial fibroblast osteogenesis is specifically induced, the interference of non-target cells is reduced, and the accuracy and reliability of the experiment are improved; the method disclosed by the invention is simple, strong in operability and good in controllability, lays a foundation for research on pathogenesis of new bone formation of ankylosing spondylitis, can greatly shorten the experimental period, and has better application prospects compared with traditional BMP-2 and TGF-beta induced fibroblasts. The osteogenesis model constructed by the method is closer to an osteogenesis mechanism of ankylosing spondylitis and is higher in conversion efficiency.

The present application belongs to the technical field of hydrogel materials, and particularly relates to a composite hydrogel for regulating energy metabolism and a preparation method and application thereof. The preparation raw material of the composite hydrogel for regulating energy metabolism comprises creatine modified double bond hydroxybutyl chitosan, hydroxyapatite, curcumin, an initiator and a solvent. CHBC provides energy metabolism support at the cell level, is conducive to the proliferation and differentiation of osteogenesis-related cells; HA provides an inorganic environment similar to bone mineralization, and plays a bone conduction and bone induction role; Cur regulates inflammatory response and improves oxidative stress state, and optimizes the repair microenvironment. Through synergy in different aspects such as energy metabolism support, structure mineralization and microenvironment regulation, the regulation of the energy metabolism state of cells in the bone repair area is realized, which is conducive to meeting the high energy requirement of osteoblasts in the repair process, and can promote new bone formation and improve the inflammatory and oxidative stress microenvironment of the injury area, and provides more favorable repair conditions for bone tissue regeneration.

The application discloses a kind of composite material based on engineering spirulina, its preparation method and application, the composite material includes hydrogel matrix and the engineering spirulina carrier dispersed in the hydrogel matrix;Wherein, the surface of spirulina is loaded with polyethyleneimine-coated Fe3O4 Nanoparticles, obtain the engineering spirulina carrier, and the engineering spirulina high expression arginine.One aspect, the present application is metabolically restructured to spirulina, so that its intracellular L-arginine content is significantly improved, L-arginine can simultaneously improve vascular function through iNOS path, and through Arg-1 path, promote macrophages to repair type M2 polarization and tissue repair, from metabolic root to intervene the core obstacle of bone defect;On the other hand, spirulina is surface modified, can effectively activate osteogenesis-related gene expression, drive new bone formation.By the double modification of L-arginine enrichment and Fe3O4 Nanoparticles loading to spirulina, obtain the composite material of synergistic regulation anti-inflammatory, pro-angiogenic and pro-osteogenic, accurately match the demand of complex pathological microenvironment of bone defect, to effectively treat bone defect, especially drug-related jaw osteonecrosis.

The invention discloses Mxene / Zn nano coating modified CF / PEEK as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, pretreating a CF / PEEK stent; s2, sulfonating the surface of the CF / PEEK stent; s3, a PEI-TA adhesive layer is constructed; s4, a CF / PEEK-MXene material is prepared; and S5, preparing the CF / PEEK-MXene coated Zn material. Through the synergistic effect of promoting osteogenesis and angiogenesis, the MXene / Zn nano-coating modified CF / PEEK stent can effectively solve the problem of mismatch of multiple biological interfaces of mechanical support-nutrition transport-cell recruitment in large-section bearing bone defect repair. The gradient mechanical adaptation characteristic of the stent can provide stable mechanical support, meanwhile, good mechanical matching with surrounding bone tissues is achieved, and the stress shielding effect is avoided. And the blood vessel-osteogenesis coupling activity ensures that the collection of cells, the transportation of nutrition and the formation of new bones can be orderly carried out in the bone repair process, so that the success rate of large-section bearing bone defect repair is greatly improved.

The invention provides a preparation method of an anti-inflammatory bone repair material for promoting angiogenesis, which comprises the following steps: adding amorphous calcium magnesium phosphate powder into a polylactic acid solution, carrying out ultrasonic treatment to uniformly disperse the amorphous calcium magnesium phosphate powder, and carrying out vacuum freeze drying to obtain the anti-inflammatory bone repair material. The mass ratio of the amorphous calcium magnesium phosphate powder to the polylactic acid is (1: 9)-(1: 1). Amorphous calcium magnesium phosphate is added into polylactic acid, so that a large amount of calcium magnesium phosphorus can be released after implantation, bone defect stem cell osteogenic differentiation and angiogenesis are promoted, and the biocompatibility of polylactic acid is improved. The addition of inorganic matters can accelerate the degradation rate of polylactic acid, and prevent the bone repair material from occupying for a long time to affect the generation of new bones. Meanwhile, the amorphous calcium magnesium phosphate can neutralize an acid environment generated by degradation of polylactic acid, and the inflammatory response of the defect part is relieved.

The application discloses a PLGA microsphere-PCL / GelMA composite scaffold based on nerve growth factor (NGF) sustained release and a preparation method and application thereof. The composite scaffold is constructed by embedding the NGF-loaded PLGA microsphere into the PCL framework and the GelMA hydrogel matrix, and a three-phase composite system with mechanical support, sustained release of biological factors and cell adhesion function is constructed. In vitro experiments show that the NGF-loaded composite scaffold significantly promotes the osteogenic differentiation of dental pulp stem cells, and the alkaline phosphatase activity is improved, the formation of mineralized nodules is increased, and the expression of osteogenesis-related genes RUNX2 , ALPL , COL1A1 and BGLAP is up-regulated. When applied to a rat skull critical defect model in vivo, the composite scaffold significantly promotes new bone formation and vascular nerve regeneration. Mechanism research shows that it realizes the regulation of osteogenesis and bone resorption by activating the PI3K / AKT signaling pathway. The composite scaffold is suitable for the repair of complex bone defects in the region rich in nerves and blood vessels, and has good clinical application prospect.

This invention belongs to the field of biomaterials technology, specifically relating to a bioactive scaffold, its preparation method, and its applications. This invention incorporates functionalized mesoporous silica nanoparticles loaded with aminoguanidine into the skin secretions of the giant salamander and polycaprolactone electrospun fibers to construct a bioactive composite scaffold (SPMA). This scaffold enables the sustained release of AG and osteogenic factors, effectively inhibiting free radical generation, AGEs toxicity, and inflammatory responses. Furthermore, it significantly improves the survival rate, osteogenic differentiation, and mineralization capacity of bone marrow mesenchymal stem cells in a high-glucose microenvironment. In vivo experiments further demonstrate that in a streptozotocin-induced diabetic mouse model of skull defects, the SPMA scaffold can alleviate local inflammation, promote angiogenesis, and significantly accelerate new bone formation and defect repair.

The application discloses a 3D printing high-strength supramolecular hydrogel and application thereof in bone defect repair. The high-strength supramolecular hydrogel is formed by hydrogen bond, ion interaction and hydrophobic association non-covalent cross-linking of a positively charged high-molecular polymer, a negatively charged functional monomer and a photoinitiator, and has a dynamic network structure. The hydrogel has a structure similar to an extracellular matrix, has self-healing, viscoelasticity and dynamics, and is beneficial to cell adhesion, proliferation, migration and differentiation. The hydrogel has superfast gelation, realizes second-level gelation solidification, and can accurately 3D print a microporous structure due to triple forces of strong hydrogen bond, electrostatic interaction and hydrophobic interaction. The hydrogel has good biodegradability, and the degradation rate can be designed by adjusting ingredients and cross-linking density, so that the degradation rate is matched with the speed of new bone formation, and finally, the scaffold material is completely replaced by new bone tissue.

The invention discloses application of PD-L1 positive mesenchymal stem cells in preparation of a medicine for inhibiting pathological osteogenesis, and belongs to the technical field of biological medicine. The medicine for inhibiting pathological osteogenesis is a medicine for preventing and / or treating diseases characterized by pathological new bone formation; the preparation method of the PD-L1 positive mesenchymal stem cells comprises the following steps: (1) providing a mesenchymal stem cell group to be screened; (2) detecting the PD-L1 expression level of each cell in the mesenchymal stem cell population to be screened; and (3) screening and enriching the PD-L1 high-expression mesenchymal stem cells according to the detection result of the step (2) to obtain the PD-L1 positive mesenchymal stem cells. The PD-L1 positive mesenchymal stem cell has a remarkable effect on inhibiting pathological osteogenesis, is a brand new treatment way, and has huge clinical value and market potential.

The invention provides a preparation method of a polyether-ether-ketone / hydroxyapatite scaffold based on 3D printing, and relates to the technical field of medical apparatuses and instruments. Comprising the following steps: firstly, constructing a cylindrical space, filling randomly distributed seed points in the cylindrical space, and carrying out surface separation and trimming between the adjacent seed points to obtain an initial bracket frame; the preparation method comprises the following steps: fully mixing polyether-ether-ketone nano powder and micron-sized hydroxyapatite powder, and drying for later use; the polyether-ether-ketone / hydroxyapatite powder is used for 3D printing, a polyether-ether-ketone / hydroxyapatite blocky material is obtained after printing, then the polyether-ether-ketone / hydroxyapatite blocky material is polished with abrasive paper, ultrasonic cleaning is conducted with ultrapure water, drying is conducted, and a polyether-ether-ketone / hydroxyapatite support is obtained; the scaffold prepared by the invention has good degradation performance and mechanical strength, and also has a significant promotion effect on osteogenesis. The polyetheretherketone / hydroxyapatite scaffold prepared by the method can be used in the field of skull repair, and can induce bone regeneration so as to promote bone tissue repair and new bone formation.

The invention provides an artificial skull restoration and relates to the technical field of biomedical materials, the artificial skull restoration comprises an inner layer, a middle layer and an outer layer which are sequentially connected from bottom to top, the inner layer is a compact structure layer, the middle layer comprises a compact frame structure and a porous loose structure, and the outer layer is a loose structure layer; the inner layer, the middle layer and the outer layer are all made of a first material or hydroxyapatite, and the first material is composed of polycaprolactone and beta-tricalcium phosphate. The artificial skull repair body provided by the invention not only has better biocompatibility and better mechanical property, but also has better degradation speed and new bone formation speed matching property, and can better promote skull tissue repair and regeneration.

The application provides a near-infrared response magnesium-releasing and antioxidant double-layer microneedle and a preparation method and application thereof. The double-layer microneedle comprises a needle tip layer and a backing layer, the needle tip layer contains MoS2@PDA@Mg 2+ The application discloses a composite particle, which is composed of molybdenum disulfide nanosheets, surface-coated polydopamine and coordination-loaded magnesium ions. The preparation method comprises the following steps: one-step reaction of molybdenum disulfide, dopamine and magnesium salt to obtain the composite particle, and then preparation of the composite particle into a needle tip layer precursor solution together with GelMA, PVA and the like, and two-step light cross-linking molding with a backing layer precursor solution. The double-layer microneedle can rapidly release magnesium ions under near-infrared irradiation, the release amount is 4.6 times that of a non-irradiation group, can significantly scavenge DPPH, ABTS and other free radicals, can relieve cell oxidative stress induced by lipopolysaccharide, and can effectively promote new bone formation and trabecular reconstruction in a rat tooth socket model. The application realizes the synergistic effect of light-controlled magnesium release and antioxidant, and is suitable for the fields of neural microenvironment reconstruction and bone regeneration.

The application discloses a histopathological evaluation device for the healing degree of bone injury, and relates to the technical field of medical devices, pharmacodynamics and pathological diagnosis. The device comprises a microscope configured to capture an image of a stained bone tissue section; an image processing unit connected to the microscope and configured to obtain semi-quantitative histological measurement results and microscopic bone histomorphometry measurement results based on the image of the stained bone tissue section; the semi-quantitative histological measurement results comprise the state of callus bridging and fracture line, the composition and maturity of callus, new bone formation activity and bone remodeling and structure recovery state; the microscopic bone histomorphometry measurement results comprise the bone volume fraction and the proportion of osteoid surface; and a host computer connected to the image processing unit and configured to generate the bone injury healing condition according to the two kinds of measurement results. The application combines semi-quantitative histological measurement and microscopic bone histomorphometry measurement, and can provide an objective and quantifiable statistical device and method for the healing effect of bone injury.

The invention discloses a PLGA microsphere-PCL / GelMA composite scaffold based on neurotrophic factor (NGF) slow release as well as a preparation method and application of the PLGA microsphere-PCL / GelMA composite scaffold. According to the composite scaffold, NGF-loaded PLGA microspheres are embedded into a PCL skeleton and a GelMA hydrogel matrix, and a three-phase composite system with mechanical support, biological factor sustained release and cell adhesion functions is constructed. In-vitro experiments show that the NGF-loaded composite scaffold can significantly promote osteogenic differentiation of dental pulp stem cells, which shows that the activity of alkaline phosphatase is improved, the formation of mineralized nodules is increased, and the expression of osteogenic related genes RUNX2, ALPL, COL1A1 and BGLAP is up-regulated. When the composite stent is applied to a rat skull critical defect model in vivo, new bone formation and vascular nerve regeneration are remarkably promoted. Mechanism research shows that regulation and control of osteogenesis and bone resorption are realized by activating a PI3K / AKT signal channel. The composite stent is suitable for repairing complex bone defects in areas with rich nerves and blood vessels, and has a good clinical application prospect.

The application relates to a preparation method and application of a bionic scaffold with bone repair and anti-osteosarcoma functions, and the specific steps are as follows: firstly, polydopamine nanoparticles are added into a curcumin ethanol solution, room temperature stirring reaction is carried out, and a polydopamine nanoparticle solution loaded with curcumin is obtained; secondly, freeze-dried silk fibroin is dissolved in hexafluoroisopropanol to form a silk fibroin solution, acetone is added to crosslink the solution into a gel, ammonium bicarbonate is added, hydroxyapatite nanoparticles are added, and mixing is uniformly carried out, and a porous fiber scaffold is synthesized through supercritical CO2 assisted phase separation; finally, the porous fiber scaffold is added into the polydopamine nanoparticle solution loaded with curcumin, room temperature stirring reaction is carried out, and then freeze-drying is carried out, so that the silk fibroin scaffold is obtained. The silk fibroin scaffold provided by the application has the anti-osteosarcoma function, can simulate the composition and structure of bone and induce new bone formation, can simultaneously meet the purposes of treating bone tumors and repairing bone defects, and has important clinical value and scientific significance.

The implant processing method disclosed in this application includes the following steps: centrifuging blood from a blood collection tube to obtain liquid autologous platelet aggregate, extracting liquid autologous concentrated growth factor from it, and injecting the liquid autologous concentrated growth factor into a liquid autologous concentrated growth factor container; placing an untreated implant into the liquid autologous concentrated growth factor container to immerse it in the liquid autologous concentrated growth factor; and centrifuging the liquid autologous concentrated growth factor and the untreated implant to obtain an implant with its surface covered by the patient's autologous concentrated growth factor, which serves as a surface-treated implant to be implanted in the patient's oral cavity. This application can provide personalized implant processing solutions based on individual patient characteristics, effectively enhancing the integration ability of the implant with bone tissue, promoting new bone formation, and thus improving the implantation outcome.

The invention discloses a 3D printing high-strength supramolecular hydrogel and application of the 3D printing high-strength supramolecular hydrogel in bone defect repair. The high-strength supramolecular hydrogel is of a dynamic network structure formed by a high-molecular polymer with positive charges, a functional monomer with negative charges and a photoinitiator through hydrogen bonds, ionic interaction and hydrophobic association non-covalent crosslinking. The hydrogel disclosed by the invention has a structure similar to an extracellular matrix, has self-healing property, viscoelasticity and dynamic property, and is beneficial to adhesion, proliferation, migration and differentiation of cells; the hydrogel disclosed by the invention has an ultra-fast gelling property, second-level gelling curing is realized, and a microporous structure can be accurately printed in a 3D manner due to triple acting forces of strong hydrogen bonds, electrostatic interaction and hydrophobic interaction; the hydrogel has good biodegradability, and the degradation rate of the hydrogel can be designed by adjusting components and crosslinking density, so that the degradation rate of the hydrogel is matched with the speed of new bone formation, and finally, a scaffold material is completely replaced by new bone tissue.

This invention belongs to the field of materials technology, specifically relating to a biomedical nanocrystalline Ti-Mg alloy coating with a micro / nano porous structure, its preparation method, and its applications. This invention constructs a nanocrystalline Ti-Mg alloy coating with a micro / nano porous structure on the surface of pure titanium or titanium alloys. Specifically, its interior contains micron-sized macropores, while its pore walls contain numerous submicron to nano-sized micropores. The framework material is nanocrystalline, thereby achieving a synergistic enhancement of the bioactivity and adjustment of the elastic modulus of the Ti-Mg alloy through porous structures of different scales. Simultaneously, the nanocrystalline structure strengthens the mechanical properties of the porous coating. Ti acts as the framework, providing mechanical support, while Mg serves as the bioactive and micro / nano-porous material. When the Ti-Mg composite material is implanted into the human body, Mg gradually degrades, which can improve the material's bioactivity (inducing new bone formation).

The present invention discloses a tricalcium phosphate bone cement doped with hydroxyapatite and a preparation method thereof, wherein the bone cement is composed of a solid phase component and a liquid phase component, wherein the solid phase component is α-tricalcium phosphate and β-tricalcium phosphate, and the addition of Mg and β-tricalcium phosphate is carried out. 2+ 、Zn 2+ 、Sr 2+ 、Cu 2+ and Si 4+ The liquid phase of this inorganic ion-doped hydroxyapatite is a mixed aqueous solution of citric acid monohydrate and disodium hydrogen phosphate dodecahydrate. Five types of doped hydroxyapatite are selected based on the trace elements present in human bone and added to bone cement. In clinical practice, the inorganic ions released as the cement degrades can significantly promote cell proliferation and new bone formation, shortening treatment time.

The invention discloses a composition for preventing and / or treating osteoporosis and application thereof, and relates to the technical field of biological medicine. The composition comprises gnetum alcohol and quercetin in a mass ratio of 1: (1-15). According to the composition disclosed by the invention, the gnetum alcohol blocks an NLRP3 / Caspase1 / GSDMD channel in a targeted manner, osteoblast inflammatory pyroptosis is accurately inhibited, and the inflammatory microenvironment of an osteoporosis region is improved from the source; the quercetin is capable of efficiently removing oxidative stress products, reducing cascade injury, directionally promoting osteoblast proliferation and differentiation and improving local bone regeneration capacity; the two components form a complementary synergistic effect, so that the pyroptosis-mediated pathological source is cut off, the injury progress is intervened, the bone repair microenvironment is synchronously improved, the new bone formation is promoted, and the limitation of single target treatment is broken through, and the curative effect is remarkably superior to that of single medication when the medicine is used for preventing and / or treating osteoporosis.

The scheme discloses the field of biomedical materials and bone tissue engineering technology, and discloses a composite drug-loaded hydrogel and a preparation method and application thereof.The composite drug-loaded hydrogel comprises a self-assembled peptide hydrogel base, hollow mesoporous dopamine nanoparticles and simvastatin, the simvastatin is loaded in the hollow mesoporous structure of the hollow mesoporous dopamine nanoparticles to form SIM@H-MPDA drug-loaded nanoparticles, and the drug-loaded nanoparticles are stably dispersed in the three-dimensional network structure of RADA16-I.The preparation method of the composite drug-loaded hydrogel is also disclosed, and the preparation method comprises H-MPDA preparation, SIM@H-MPDA drug-loaded nanoparticle preparation and composite hydrogel assembly.The composite drug-loaded hydrogel has good injectability and in-situ gelation, can realize smooth and slow release of simvastatin, significantly promotes osteogenic differentiation and new bone formation, effectively repairs non-infectious bone defects, and has no adverse effects on main organs of the body.

The invention belongs to the field of biomedical polymer materials, and discloses a preparation method and application of an epitope imprinting bionic receptor for self-recruitment of endogenous osteogenic growth peptide. According to the invention, from the angle of collection of endogenous growth factors, the OGP is taken as a target molecule, the epitope imprinting polymer nanoparticles MIP with specific recognition capability are prepared by adopting an epitope imprinting technology, and specific capture of the endogenous OGP is realized. Further, according to the invention, the MIP is introduced into a polyethylene glycol PEG hydrogel system, and the epitope imprinting bionic receptor MIP (at) PEG capable of collecting endogenous osteogenic factors is prepared. Directional capture and slow release of OGP and activation of local osteogenesis signals are realized in vivo, and formation of new bones in a bone defect area is remarkably promoted. The invention provides a new thought and experimental basis for developing a novel tissue engineering scaffold based on endogenous growth factor recruitment, and has important clinical application prospects.