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Artificial cornea and preparation method thereof

A technology of artificial cornea and corneal stroma, applied in the field of medical materials, can solve problems such as the disease is not prevented, and achieve the effects of improving mechanical strength, improving biomechanical properties, and improving biomechanical strength and stability

Active Publication Date: 2016-02-24
GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above, the photooxidative collagen cross-linking method is mainly used clinically as a therapy for treating diseases. It is relatively safe as a therapy, but due to the influence of some patients with advanced disease or postoperative complications, the disease has not been prevented, or There are certain defects due to the limitation of treatment depth

Method used

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  • Artificial cornea and preparation method thereof
  • Artificial cornea and preparation method thereof
  • Artificial cornea and preparation method thereof

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preparation example Construction

[0034]The preparation method of a kind of artificial cornea of ​​the present invention mainly uses animal decellularized corneal stroma as the decellularized scaffold, wherein the photosensitizer that can absorb light and transmit the absorbed light energy to the reactant and has no toxicity or low toxicity to human body or animals is used. agent for photooxidative crosslinking. Under the condition of light irradiation (visible light or ultraviolet light irradiation), the photosensitizer is excited to the triplet state, and releases the active oxygen group mainly composed of singlet oxygen, and the active oxygen is chemically active and extremely unstable, and can interact with various This molecular reaction induces a chemical cross-linking reaction between collagen fiber molecular groups (such as amino groups) (type II photochemical reaction), forming new molecular chains inside or between collagen fibers, and improving the mechanical strength of collagen fibers. The photose...

Embodiment 1

[0038] A kind of preparation method of artificial cornea, the steps that it comprises are as follows:

[0039] (1) Soak the animal decellularized corneal stroma in a photosensitizer solution with a concentration of 0.05 mg / ml for 30 minutes;

[0040] (2) Take out the soaked animal acellular corneal stroma and put it in a petri dish, and irradiate it with 110nm wavelength ultraviolet light for 40 minutes; change the surface when the ultraviolet light is irradiated for 20 minutes, and then continue to irradiate, and the power density of the irradiated ultraviolet light is 3mw / cm 2 ;

[0041] (3) Soak in PBS solution for 0.6h, then shake for 0.6h;

[0042] (4) Change the PBS solution and soak for 0.6h, then shake for 0.6h.

Embodiment 2

[0044] A kind of preparation method of artificial cornea, the steps that it comprises are as follows:

[0045] (1) Soak the animal decellularized corneal stroma in a photosensitizer solution with a concentration of 4.5 mg / ml for 55 minutes;

[0046] (2) Take out the soaked animal acellular corneal stroma and put it in a culture dish, and irradiate it with 380nm wavelength ultraviolet light for 56 minutes; change the surface when irradiating for 28 minutes, and then continue to irradiate; Density at 5800mw / cm 2 ;

[0047] (3) Soak in PBS solution for 4 hours, then shake for 2 hours;

[0048] (4) Replace the PBS solution, soak for another 4 hours, and shake for 2 hours.

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Abstract

The invention discloses an artificial cornea, made by soaking animal decellularized corneal stroma in a photosensitizer first and performing uniform crosslinking in ultraviolet light. A photo-oxidation collagen crosslinking method comprises following steps: (1), soaking animal decellularized corneal stroma in a photosensitizer solution 1-5 mg / ml for 1-60 min; (2), taking the soaked animal decellularized corneal stroma, placing it in a culture vessel, and exposing it to ultraviolet light 100-400 nm in wavelength for 1-60 min; (3), soaking the stroma in a PBS solution for 0.5-4 h, and shaking for 0.5-2 h. The invention has the advantages that the biomechanic characteristics such as mechanical strength of the animal decellularized corneal stroma can be effectively improved; as the biomechanic characteristics are mainly determined by collage fiber, collagen fiber bundles and the composition of their spatial structures, the biomechanic properties and stability of the animal decellularized corneal stroma can be improved through photo-radiation / photosensitizer crosslinking.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to an artificial cornea and a preparation method thereof. Background technique [0002] The basic principle of photochemical collagen crosslinking is photooxidation reaction. According to the unique absorption spectrum of the photosensitizer, within its maximum absorption wavelength range, after being irradiated with a certain wavelength, the photosensitizer absorbs photon energy, is excited to form a triplet state, has a strong oxidation ability, and produces superoxide radicals or singlets. Oxygen, oxygen free radicals have the activity of directly modifying collagen molecules, causing the formation of covalent bonds in or between amino acid residues in collagen molecules, increasing the density of covalent bonds between collagen fibers, thereby improving the biomechanical properties of collagen (type II photochemical reaction). With the continuous research on photooxidation rea...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 官习鹏易静楠
Owner GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
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