Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of photooxidative collagen cross-linking method and its application

A collagen cross-linking and photo-oxidation technology, applied in the field of medical supplies, can solve the problem of unprevented disease, achieve low immunogenicity, improve biomechanical properties, and improve mechanical strength.

Active Publication Date: 2018-06-22
GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above, the photooxidative collagen cross-linking method is mainly used clinically as a therapy for treating diseases. It is relatively safe as a therapy, but due to the influence of some patients with advanced disease or postoperative complications, the disease has not been prevented, or There are certain defects due to the limitation of treatment depth

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of photooxidative collagen cross-linking method and its application
  • A kind of photooxidative collagen cross-linking method and its application
  • A kind of photooxidative collagen cross-linking method and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for photooxidative collagen cross-linking, which comprises the following steps:

[0040] (1) Soak the decellularized scaffold in a photosensitizer solution with a concentration of 0.05 mg / ml for 30 min;

[0041] (2) Take out the soaked decellularized scaffold and place it in a petri dish, and irradiate it with 110nm wavelength ultraviolet light for 40 minutes; change the surface when the ultraviolet light is irradiated for 20 minutes, and then continue to irradiate. The power density of the irradiated ultraviolet light is 3mw / cm 2 ;

[0042] (3) Soak in PBS solution for 0.6h, then shake for 0.6h;

[0043] (4) Soak in PBS solution for 0.6h, then shake for 0.6h.

Embodiment 2

[0045] A method for photooxidative collagen cross-linking, which comprises the following steps:

[0046] (1) Soak the decellularized scaffold in a photosensitizer solution with a concentration of 4.5mg / ml for 55min;

[0047] (2) Take out the soaked decellularized scaffold and put it in a petri dish, and irradiate it with 380nm wavelength ultraviolet light for 56min; change the surface when irradiating for 28min, and then irradiate; in the step (2), the power density of irradiation is 5800mw / cm 2 ;

[0048] (3) Soak in PBS solution for 4 hours, then shake for 2 hours;

[0049] (4) Soak in PBS solution for 4 hours, then shake for 2 hours.

Embodiment 3

[0051] A method for photooxidative collagen cross-linking, which comprises the following steps:

[0052] (1) Soak the decellularized scaffold in 0.2mg / ml riboflavin sodium phosphate solution for 50min;

[0053] (2) Take out the soaked decellularized scaffold and place it in a petri dish, and irradiate it with 365nm wavelength ultraviolet light for 30min; the power density of irradiation is 3000mw / cm 2 ; Change the surface at 15 minutes, try to ensure that the front and back sides are cross-linked evenly.

[0054] (3) Soak in PBS solution for 3 hours, then shake for 1 hour;

[0055] (4) Soak in PBS solution for 3 hours, then shake for 1 hour.

[0056] In the above embodiments, the decellularized scaffold may be a product of decellularized animal tissues such as pigs, cattle, sheep, horses, rabbits, and monkeys. The photosensitizer can be NFK, 3-hydroxykynuric acid (3-OH-FK) β-porphyrin, riboflavin, FMN, FAD, retinal and other pigment photosensitizers. The photosensitizer ca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
absorbanceaaaaaaaaaa
Login to View More

Abstract

The invention discloses a photo-oxidation collagen crosslinking method, comprising following steps: (1), soaking a decellularized scaffold in a riboflavin sodium phosphate solution 0.01-5 mg / ml for 1-60 min; (2), taking out the soaked decellularized scaffold, placing it in a culture vessel, exposing it to ultraviolet light 100-400 nm in wavelength for 1-60 min; (3), soaking the scaffold in a PBS solution for 0.5-4 h, and shaking for 0.5-2 h. The photo-oxidation collagen crosslinking method is used for preparing medical substitute materials. The method has the advantages that biomechanic characteristics such as mechanical strength of the decellularized scaffold can be effectively improved; as the biomechanic characteristics are mainly determined by collage fiber, collagen fiber bundles and the composition of their spatial structures, the biomechanic properties and stability of a decellularized material can be improved through photo-radiation / photosensitizer crosslinking.

Description

technical field [0001] The invention relates to the field of medical supplies, in particular to a method and application of photooxidized collagen cross-linking for treating decellularized tissues. Background technique [0002] The basic principle of photochemical collagen crosslinking is photooxidation reaction. According to the unique absorption spectrum of the photosensitizer, within its maximum absorption wavelength range, after being irradiated with a certain wavelength, the photosensitizer absorbs photon energy, is excited to form a triplet state, has a strong oxidation ability, and produces superoxide radicals or singlets. Oxygen, oxygen free radicals have the activity of directly modifying collagen molecules, causing the formation of covalent bonds in or between amino acid residues in collagen molecules, increasing the density of covalent bonds between collagen fibers, thereby improving the biomechanical properties of collagen (type II photochemical reaction). With...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/24A61L27/50
Inventor 官习鹏易静楠
Owner GUANGZHOU YUEQING REGENERATION MEDICINE TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com