34results about How to "Improve biomechanical properties" patented technology

The invention discloses a gradient mineralized bone extracellular matrix material and a preparation method thereof. The method comprises the following steps of: carrying out immunogenicity removal treatment, namely decellularization on bone tissues derived from natural tissues, and carrying out gradient demineralization treatment on the obtained decellularized bone so as to obtain the gradient mineralized bone extracellular matrix material. The method enlarges the porosity of the bone matrix material and the exposure degree of collagen on the surface of the bone matrix material, effectively releases growth factors, well improves the adhesion degree of the material to cells, and regulates and controls the up-regulation of genes and proteins related to cell regeneration. The method not onlywell retains the biomechanical characteristics and the three-dimensional microstructure of a natural bone ECM scaffold, but also plays a positive role in osteogenesis, angiogenesis and collagen mineralization in the early stage of fracture, thereby increasing the colonization adhesion of cells and promoting the differentiation induction of the cells. The gradient mineralized bone extracellular matrix material derived from natural tissues exhibit greater potential in promoting mesenchymal stem cell differentiation and osteogenesis than non-demineralized or fully-demineralized bone matrix materials.

The invention belongs to the technical field of in-vitro establishment of tissue engineering cartilages, more particularly to an experimental method for establishing tissue engineering cartilage in vitro by taking a knee-joint cartilage unit of a rabbit as a seed cell. The experimental method comprises the following steps of: taking a keen joint of a rabbit, repeatedly cutting the joint cartilage into fragmentized tissues, washing, removing supernatant away and putting in a sterile conical flask for later use; adding dispase and II-type collagenase into the conical flask according to 12mL DMEM-F12 culture solution / g cartilage, stirring and digesting to form a digested suspension solution; filtering and centrifuging the digested suspension solution, adding a primary chondrocyte culture solution to prepare a sterile chondrocyte suspension solution, and carrying out sodium alginate gel three-dimensional culture on the obtained cartilage unit to construct the tissue engineering cartilage. The invention has the beneficial effects that: the cartilage unit has stable form and multiplication situation in the in-vitro long-term culturing process, the physiological function of substrate ingredients, such as secreted II-type collagen, and the like is improved, and the effect of repairing cartilage injury by adopting a tissue engineering method is expectedly accelerated.

The invention discloses a composition containing oligopeptide and D-glucosamine hydrochloride for patients with bone injury. The composition contains oligopeptide, D-glucosamine hydrochloride and a traditional Chinese medicine extract, wherein the oligopeptide is the mixture of marine collagen peptide and marine ossein peptide, and the traditional Chinese medicine extract is the extract of peach kernel, lentinus edodes and dried orange peel. The composition not only can maintain the nutritive equilibrium of patients with bone injury, but also can improve the biomechanical characteristics of compression resistance and fracture resistance of patients with osteoporosis.

The invention relates to a biological patching material for patching defective tissues or organs in various surgical operations, which is characterized in that a composition is prepared from collagen or / and fibroin and polylactic acid or / and polycaprolactone in percentage by mass by electrostatic spinning technology. The obtained biological patching material has the advantages of good biological mechanical property and biocompatibility, convenient preparation and low cost, can be prepared into random specifications, can be remolded and degraded in vivo, and can be used for various surgical patching operations.

The invention discloses a photo-oxidation collagen crosslinking method, comprising following steps: (1), soaking a decellularized scaffold in a riboflavin sodium phosphate solution 0.01-5 mg / ml for 1-60 min; (2), taking out the soaked decellularized scaffold, placing it in a culture vessel, exposing it to ultraviolet light 100-400 nm in wavelength for 1-60 min; (3), soaking the scaffold in a PBS solution for 0.5-4 h, and shaking for 0.5-2 h. The photo-oxidation collagen crosslinking method is used for preparing medical substitute materials. The method has the advantages that biomechanic characteristics such as mechanical strength of the decellularized scaffold can be effectively improved; as the biomechanic characteristics are mainly determined by collage fiber, collagen fiber bundles and the composition of their spatial structures, the biomechanic properties and stability of a decellularized material can be improved through photo-radiation / photosensitizer crosslinking.

The invention relates to the technical field of medical biological materials, in particular to a bioactive degradable coral hydroxyapatite artificial bone and a preparation method thereof. The artificial bone comprises a bracket, wherein bone-inducing active cell factors and controlled release formulations are attached onto the bracket; the bracket comprises a bracket body; the bracket body is coral; a hydroxyapatite layer coats the surface of the bracket body; the bone-inducing active cell factors are made of recombinant human bone morphogenetic protein-2 mature peptide; the controlled release formulations are selected from collagen, fibronectin, hyaluronic acid and the like; the bracket is granular or cylindrical; the aperture range of micropores is 77-180 microns; the porosity of the micropores is 8-11%. According to verification results of the National Institute for the Control of Pharmaceutical and Biological Products (verification report numbers comprise QZ200701428 and QZ200804480) and the National Institutes for Food and Drug control (verification report number is QZ201206312), the artificial bone is excellent bone graft substitute.

The invention relates to the field of biological fiber materials. The conventional tendon and ligament substituent mainly comprises autograft, allograft or heteroplastic graft and synthetic material graft which all have unavoidable defects. The invention aims to provide a natural biological material with excellent biomechanical properties and biocompatibility, namely an acellular tendon / ligament collagenous fiber material, and a preparation method thereof. In the preparation method, a mechanical structural unit and a cell attachment structure which tendon / ligament collagenous fibers or fiber bundles originally have are reserved; the tendon / ligament fibers are processed by adopting a low-temperature drying method, and the separated fibers reserve certain mechanical strength; and the collagenous fibers or fiber bundles are selected by a physical loosening and carding method and then acellular treatment is performed. The tendon / ligament acellular collagenous fiber / fiber bundle material of the invention has wide source, large quantity and low price and not only can be used for ligament and tendon reconstruction and rehabilitation, but also can be widely applied to other biomedical fields because the tendon / ligament acellular collagenous fiber / fiber bundle material has properties of the fiber material.

Bilateral positioning type pedicle screw aimer and its preparation method, the preparation method includes the following steps: acquiring medical image data of the target vertebra; reconstructing the three-dimensional model and center point set of the target vertebra; creating a one-time screw insertion to be corrected line; create the secondary screw entry line; determine the diameter of the largest inscribed cylinder of the pedicle along the direction of the secondary screw entry line; determine the value range of the diameter of the pedicle screw and the screw channel; establish a three-dimensional model of the body; manufacture Bilaterally positioned pedicle screw aimer. By adopting the preparation method of the present invention, a bilateral positioning type pedicle screw sighter with high-precision screw setting channels can be manufactured, which is well attached to the target vertebra positioning curved surface. With the aid of the aiming device, the operator can accurately and quickly perform the internal fixation of the pedicle screw, which not only improves the operation efficiency, but also ensures the accuracy and safety of the operation.

The invention discloses a design method of a personalized condylar prosthesis, which is specifically implemented according to the following steps: Step 1, using CBCT to shoot the medical image data of the patient's maxillofacial region; importing the data into the medical image processing software Mimics in DICOM format to generate Whole head 3D virtual model; separate the mandible model from the whole head model, and output it as an STL format file; Step 2, import the STL file of the mandible model into Geomagic software, simulate the operation process to perform osteotomy of the lesion area, and then perform Trimming to obtain the STL model of mandible a; Reverse reconstruction of the STL model of mandible a, then forward design, fine adjustment of the image, and precise surface fitting using the principle of quadrilateral distribution to obtain the condyle prosthesis The STL model, that is, the desired personalized condylar prosthesis. The invention solves the problem that the condyle prosthesis in the prior art needs to grind away a large amount of bone tissue to adapt to the shape of the prosthesis.

A personalized pedicle screw drilling mold with quick-change drill sleeves and a preparation method thereof. The personalized pedicle screw drilling mold includes a body and a drill sleeve; There is a nailing plane, and there are finger pressure pits on the front and rear side walls. There are two nailing channels in the body. One end of the nailing channel is connected to the nailing plane, and the other end is connected to the bottom surface; the drill sleeve is installed in the nailing channel. , they form a transition fit. The advantage of the present invention is that the structural size and the bone-fitting surface of the body are designed according to the shape and size of the target vertebra and the positional relationship with adjacent vertebrae, which has obvious characteristics of personalized customization. Drill the threaded bottom hole of the pedicle screw through the drill sleeve, and then implant the pedicle screw, which can not only ensure that the pedicle cortex is not pierced, but also ensure that the screw has a larger diameter within the range allowed by the pedicle space. Diameter, so as to ensure a firm three-dimensional fixation effect after implantation to the greatest extent, and realize the reproduction of the biomechanical characteristics of the vertebral joint.

The invention discloses a Chinese medicinal composition for accelerating fracture healing, and relates to the technical field of Chinese medicaments. The Chinese medicinal composition is prepared from the following Chinese medicinal materials in parts by weight: 12 to 15 parts of frankincense, 12 to 15 parts of myrrh, 12 to 15 parts of Chinese angelica, 4 to 6 parts of asarum, 12 to 18 parts of dragon's blood, 10 to 14 parts of garden balsam stem, 8 to 12 parts of pseudo-ginseng, 12 to 15 parts of beautiful sweetgum fruit, 12 to 15 parts of unprocessed kusnezoff monkshood root, and 4 to 8 parts of borneol. The Chinese medicinal composition related by the invention has an effect of improving the anti-compression anti-fracture biomechanical character of a patient with osteoporosis.

The invention discloses an elasticity modulus determination method and a preparation method of an individual posterior spinal pedicle screw rod system. The elasticity modulus determination method comprises the steps that CT scanning is performed on a target spine, and a three-dimensional model is rebuilt; material properties are supplied, a finite element analysis model of the target spine is built, and an elasticity modulus gradient distribution law of the target spine is obtained through analysis; the elasticity modulus of the pedicle screw rod system is obtained for the assembly relationship between the target spine and the pedicle screw rod system by combining the elasticity modulus gradient distribution law of the target spine and adopting an interpolation method. The preparation method comprises the steps that the pedicle screw rod system of which the elasticity modulus meets the requirement is prepared by adopting a metal sintering technique. The pedicle screw rod system is well matched with the biomechanical property of the individual spine, pedicle screw and rod stress distribution is more uniform and reasonable, the fixing requirement is better met, and a scientific basis can be supplied to next research and development of a novel material, optimization design of surgical methods and surgical instruments and building of a relevant database.