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Biological repair mesh and preparation method and application thereof

A mesh and biological technology, applied in the field of biological repair mesh and its preparation, can solve problems such as mechanical strength decline, body recurrence, and immune safety problems

Active Publication Date: 2020-07-14
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, after the traditional biological patch is implanted in the body, it will react with the body and begin to degrade, resulting in a decrease in mechanical strength, which will lead to the recurrence of the body.
In addition, the biological patch is ruptured due to local stress concentration in the body, and the problem of immune safety is also the cause of recurrence.

Method used

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  • Biological repair mesh and preparation method and application thereof
  • Biological repair mesh and preparation method and application thereof
  • Biological repair mesh and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0036] see figure 1 , the preparation method of the biological repair mesh of one embodiment, comprises the following steps:

[0037] Step S110: Slice the animal tendon tissue along the fiber axis, then freeze and thaw repeatedly, and roll and age after rethawing to obtain collagen fiber bundles.

[0038] Wherein, the animal is a mammal. The content of type I collagen is the highest in the tendon tissue of animals, and the fiber bundles are arranged in an orderly manner, while other tissues such as ligaments, peritoneum, and dermis contain elastic fibers and type III collagen, which are non-directional and disorderly arranged. The directional arrangement of collagen fiber bundles provides the basis for the subsequent steps of spreading and weaving.

[0039] Specifically, the slices have a thickness of 1 mm to 5 mm. The number of times of repeated freezing and thawing was 3 to 8 times. During the repeated freezing and thawing steps, the freezing temperature is -80°C to -60°...

Embodiment 1

[0072] The preparation process of the biological repair mesh of the present embodiment is as follows:

[0073] (1) Preparation of collagen fiber bundles: the animal tendon tissue was sliced ​​along the fiber axis, with a slice thickness of 3 mm. Repeated low-temperature freeze-thaw 5 times, low-temperature quick-freezing temperature: -80°C. After remelting, carry out rolling for 2 times, aging at 4°C for 4 hours after rolling each time, and separate fiber bundles after aging to remove broken fiber bundles.

[0074] (2) Collagen fiber bundles to remove immunogenic factors: first soak collagen fiber bundles in 0.1M sodium hydroxide solution for 8 hours, then wash them with purified water until neutral; then use 1.5% TritonX-100 aqueous solution to soak collagen Fiber bundles for 4 hours, then washed with purified water; then soak collagen fiber bundles with 0.2M hydrochloric acid solution for 8 hours, and then washed with purified water until neutral; finally use Tris lye with ...

Embodiment 2

[0078] The preparation process of the biological repair mesh of the present embodiment is as follows:

[0079] (1) Preparation of collagen fiber bundles: the animal tendon tissue was sliced ​​along the fiber axis, with a thickness of 5 mm. Repeated low-temperature freezing and thawing 8 times, low-temperature quick-freezing temperature: -60°C. After remelting, roll pressing is carried out once, and after each roll pressing, it is aged at 2°C for 2 hours. After aging, fiber bundles are separated to remove broken fiber bundles.

[0080] (2) Collagen fiber bundles to remove immunogenic factors: first use 1.0M sodium hydroxide solution to soak collagen fiber bundles for 4 hours, then wash with purified water until neutral; then use 2% Tween-60 aqueous solution to soak Collagen fiber bundles, the time is 6h, then washed with purified water; then use 0.5M acetic acid solution to soak the collagen fiber bundles, time is 6h, and then wash with purified water until neutral; finally us...

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Abstract

The invention relates to a biological repair mesh and a preparation method and application thereof. The preparation method of the biological repair mesh comprises the following steps of: slicing animal tendon tissues along the axial direction of fibers, then carrying out repeated freezing and thawing, and after repeated freezing and thawing, carrying out rolling and ageing to obtain a collagenousfiber bundle; carrying out decellularization, removal of DNA, removal of an alpha-Gal antigen an removal of a soluble hybrid protein on the collagenous fiber bundle by adopting chemical reagents to obtain the collagenous fiber bundle subjected to removal of immunogenicity; carrying out freeze-drying on the collagenous fiber bundle subjected to removal of immunogenicity, and then carrying out crosslinking under the vacuum condition and at a temperature of 100 DEG C to 115 DEG C to obtain the cross-linked collagenous fiber bundle; and carrying out rolling and fiber expansion on the cross-linkedcollagenous fiber bundle, and then carrying out weaving to obtain the biological repair mesh. By the preparation method of the biological repair mesh, the biological repair mesh with high immunizationsafety and a good mechanical property can be prepared.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a biological repair mesh and its preparation method and application. Background technique [0002] Traditional patch materials are divided into synthetic patches and biological patches. The principle of synthetic mesh is to form a firm fibrous tissue complex like a scar centered on the mesh in the defect area. However, the elasticity of the local tissue is reduced. If there is too much scar-like fibrous connective tissue, the patient will often feel tightness, pulling or foreign body sensation after surgery. Some patients may even have problems such as serous swelling, infection, chronic pain, patch shrinkage, intestinal adhesion, intestinal obstruction, intestinal fistula and recurrence. [0003] Biological patch is an extracellular matrix fibrous scaffold composed of collagen, elastic fibers, glycoproteins, cohesin, and proteoglycan. The main feature is that it has no blo...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/56A61L27/58
CPCA61L27/3604A61L27/3641A61L27/3687A61L27/3645A61L27/3691A61L27/56A61L27/58A61L2430/34
Inventor 魏欣苗佘振定谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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