Method for preparing collagen/bioglass/hyaluronic acid tissue repairing materials

A bio-glass, hyaluronic acid technology, applied in medical science, prosthesis and other directions, can solve the problems of low mechanical properties and anti-degradation performance, and achieve the effects of improving mechanical strength, increasing mechanical strength, and enhancing aggregation state.

Active Publication Date: 2009-12-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the composite material obtained by this preparation method has good bioactivity and biocompatibility, its mechanical properties and degradation resistance are still low.

Method used

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  • Method for preparing collagen/bioglass/hyaluronic acid tissue repairing materials
  • Method for preparing collagen/bioglass/hyaluronic acid tissue repairing materials
  • Method for preparing collagen/bioglass/hyaluronic acid tissue repairing materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Compounding of type I collagen and bioactive glass:

[0045] In the state of stirring the type I collagen solution, add CaO-P in the ratio of type I collagen to bioactive glass mass ratio of 0.33:1 2 o 5 -SiO 2 Department of bioglass, then stirred for 2 hours to obtain a mixture of the two, in which CaO-P 2 o 5 -SiO 2 The bioactive glass is prepared by the sol-gel method;

[0046] (2) Static layering:

[0047] Put the mixture of type I collagen and biological glass at 4°C for 3 days, and obtain the white flocculent precipitate of the mixture;

[0048] (3) Suction filtration:

[0049] First filter the white flocculent precipitate in step (2), until the volume of the precipitate is reduced to 1 / 4 of the original volume, that is, a viscous liquid with interlaced distribution of collagen fibers is formed, and then the viscous Stir the thick liquid until uniform;

[0050] (4) Adjust the pH value:

[0051] Add biological buffer agent 2-(N-morpholine)ethanesulfonic...

Embodiment 2

[0062] (1) Compounding of type I collagen and bioactive glass:

[0063] In the state of stirring the type I collagen solution, add Na 2 O-CaO-P 2 o 5 -SiO 2 Department of biological glass, then stirred for 2 hours to obtain a mixture of the two, in which Na 2 O-CaO-P 2 o 5 -SiO 2 The bioactive glass is prepared by melting method;

[0064] (2) Static layering:

[0065] Put the mixture of type I collagen and biological glass at 0°C for 1 day, and obtain the white flocculent precipitate of the mixture;

[0066] (3) Suction filtration:

[0067] First filter the white flocculent precipitate in step (2) until the volume of the precipitate is reduced to 1 / 2 of the original volume, that is, a viscous liquid with interlaced distribution of collagen fibers is formed, and then the viscous Stir the thick liquid until uniform;

[0068] (4) Adjust the pH value:

[0069] Add the biological buffer Tris hydrochloride to the above homogeneous viscous liquid to adjust the pH to 9;

...

Embodiment 3

[0079] (1) Compounding of type I collagen and bioactive glass:

[0080] In the state of stirring the type I collagen solution, add CaO-P at a mass ratio of type I collagen to bioactive glass of 1:1 2 o 5 -SiO 2 Department of bioglass, then stirred for 3 hours to obtain a mixture of the two, in which CaO-P 2 o 5 -SiO 2 The bioactive glass is prepared by the sol-gel method;

[0081] (2) Static layering:

[0082] Put the mixture of type I collagen and biological glass at 1°C for 3 days, and obtain the white flocculent precipitate of the mixture;

[0083] (3) Suction filtration:

[0084] First filter the white flocculent precipitate in step (2), until the volume of the precipitate is reduced to 1 / 4 of the original volume, that is, a viscous liquid with interlaced distribution of collagen fibers is formed, and then the viscous Stir the thick liquid until uniform;

[0085] (4) Adjust the pH value:

[0086] Add biological buffer agent 2-(N-morpholine)ethanesulfonic acid to ...

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Abstract

The invention discloses a method for preparing collagen/bioglass/hyaluronic acid tissue repairing materials, comprising the following steps: combining I-type collagen and bioglass, static layering, filtering, adjusting pH value, adding hyaluronic acid, cross linking, molding, freezing and drying and the like. In the process of preparing the collagen/bioglass/hyaluronic acid tissue repairing materials, the diameter of the collagenous fiber bundle composing the network structure of the tissue repairing materials is increased by adjusting the ratio of the I-type collagen and bioglass and the pH value of the combined solution, and the microstructure of the materials is adjusted, thus achieving the aim of improving mechanical strength thereof. The collagen/bioglass/hyaluronic acid tissue repairing materials prepared by the invention has improvement in mechanical property and degradation performance, can be widely used in medical field, also can be used in tissue engineering scaffolds and bone defects and soft tissue injuries repairing.

Description

technical field [0001] The invention relates to the technical field of human body repair materials, in particular to a method for preparing a collagen / biological glass / hyaluronic acid (Col / BG / HYA) tissue repair material capable of improving the strength of the repair material. Background technique [0002] Collagen is a kind of extracellular matrix (ECM), which belongs to structural protein, accounts for about 1 / 3 of the total protein mass of mammals, and is the main component of connective tissues or organs such as skin, ligament, cartilage, tendon, etc. Organs, important structural proteins that protect body functions. Type I collagen is the collagen formed by fibers and is the main structural macromolecule of the extracellular matrix of multicellular organisms. It is very important for maintaining the integrity of bone structure and bone biomechanical properties. The introduction of hyaluronic acid into collagen can comprehensively exert the advantages of two biomacromol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/56A61L27/54A61L27/24A61L27/10A61L27/20
Inventor 陈晓峰孟永春王迎军赵娜如吴刚徐彩霞苗国厚张娟娟
Owner SOUTH CHINA UNIV OF TECH
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