111 results about "Basal lamina" patented technology

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.

An object of the invention is to provide a method for delivery of macromolecules into biological cells, such as Langerhans cells (22) in the epidermis (20) of a patient, which includes the steps of coating electodes (16) in an electrode assembly (12) with solid phase macromolecules to be delivered, such as a DNA, and/or RNA vaccine or a protein-based vaccine, attaching the electrode assembly (12) having the coated electrodes (16) to an electrode assembly holder (13), providing a waveform generator (15), establishing electrically conductive pathways between the electrodes (16), and the waveform generator (15), locating the electrodes (16) such that the biological cells are situated therebetween, such as by penetrating the needle electrode (16) into the epidermis (20) above the epidermal basal lamina, and providing pulse waveform from the waveform generator (15) to the electrodes (16), such that macromolecule on the electrodes (16) is driven off of the electrodes (16), and delivered into the biological cells, such as the Langerhans cells (22).

A multilayer microperforated implant comprising a plurality of microperforated substrate layers is provided. Methods of forming a microperforated implant comprise providing at least one substrate layer; perforating at least a region of the substrate layer; and dehydrating the substrate layer. Methods of augmenting a site in need of repair with the microperforated implant are also provided.

Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated, and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein.

The invention relates to a shifted Al-plating paint for offset printing, a preparation method and application thereof. The paint provided by the invention comprises the following components of: by weight, 5-25 parts of cellulosic resin, 3-12 parts of polyacrylic resin, 0-5 parts of Vinyl chloride-acetate copolymer, 0-0.5 parts of an auxiliary agent and 70-86 parts of a solvent. The prepared shifted Al-plating paint can be used in the offset printing technology. In comparison with the prior art, the shifted Al-plating paint can be directly used for offset printing without the varnishing process, has excellent adhesive fastness with offset printing ink; the preparation method provided by the invention has advantages of simple technology and high production efficiency; the paint also has excellent peeling performance and good bonding fastness with an aluminium layer and a printing ink layer, and the PET basal lamina can be repeatedly used; in addition, the solvent used in the preparation of the paint contains no industrial forbidden solvent such as BTX, is safe and environmentally-friendly, and is beneficial for sustainable development.

The invention discloses a soaking and coating method of artificial vessel silk protein, which is synthesized by woven or knitted elastic porous gap structure to coat at least five times through silk protein, wherein the false former of silk protein liquid vessel needs composite reinforcer, which is coated in the base layer evenly to expose in the formaldehyde steam after drying.

There is provided a nail enamel strip having a substrate layer and a nail enamel film with one or more nail enamel layers, at least one of the nail enamel layers having a cross-linked polymer. The nail enamel film is detachably adhered to the substrate layer. Further, there is provided a method for making the nail enamel strips, and a method of applying the nail enamel strip on a nail.

The invention discloses a substrate offset diagnosis and correction method, which comprises a diagnosis step (10) and a correction step (20) as follows: in the diagnosis step, determining whether the substrate carried by a transmission part is offset based on the detection result of a first sensor; in case of no offset, do not carry out the following correction step; in case of offset, carrying out the correction step; in the correction step, adjusting the location of the substrate, and then enabling the substrate to eliminate offset and return to the baseline position. In addition, the invention also provides a substrate offset diagnosis and correction device which comprises a diagnosis unit and a correction unit, wherein, the diagnosis unit is used to judge whether the substrate carried by the transmission part is offset; in case of offset, triggering the correction unit to work; in case of no offset, do not carry out any operation; the correction unit is used to adjust the location of the substrate and enable the substrate to eliminate offset and return to the baseline position. The method and the device are simple to use and enable the substrate to be accurately transmitted; besides, the device has high transmission accuracy and good reliability.

A mat having a base layer that is bonded to a top layer. The base layer of the mat includes a plurality of depressions along the bottom surface of the base layer, wherein the depth of the depressions does not exceed the depth of the base layer. The orientation of the depressions on the bottom surface of the mat is such that there is a greater concentration of depressions along the edges of the mat. The top layer of the mat is preferably a layer of carpet tuft. In operation, the depressions of the mat suction the mat to the surface onto which the mat is placed once an initial force is applied to the mat, such as a footstep or vehicular traffic.

The invention relates to a gas diffusion layer for a proton exchange membrane fuel cell, wherein, the gas diffusion layer is composed of a basal cell layer and a microporous layer, wherein, the total weight percentage content of a hydrophobic agent in the basal cell layer is 1-50 percent; and the hydrophobic nature in the basal cell layer shows the gradient decrease from the side of the microporous layer to the side of a flow field. The preparation steps are as follows: a) the hydrophobic agent is evenly dispersed in a solvent to form slurry; b) the slurry is evenly prepared to the surface of a porous conductive basal cell layer material from one side, the slurry is sunk into the basal cell layer material under the action of gravity and viscous force, thereby realizing the gradient distribution in the basal cell layer; c) the basal cell layer of a gas diffusion electrode is formed by baking for 20-40 minutes at 300-400 DEG C. The gas diffusion layer is applied to hydrogen-oxygen proton exchange membrane fuel cells and direct alcohol fuel cells, as well as cells, electrolytic cells and sensors with the gas diffusion electrode structures. As the gas diffusion layer adopts the gradient hydrophobization, the gas diffusion layer is conductive to the discharge of water which is a cathode product and improves the cell performance.

The invention relates to a preparation method of a nano micrometer structure coexistence chitosan double-layer support, which belongs to the biology medical material field. The preparation method is characterized in that a phase separating technology is used to prepare a three-dimensional micrometer chitosan support as a basal layer, a high voltage static static spinning technology is used for spinning nano chitosan fiber on the micrometer chitosan surface, and thereby the nano micrometer structure coexistence chitosan double-layer support can be obtained. The prepared double layer support has good mechanical strength and high amount of porosity, and is suitable for adhesion, growth and differentiation of cells. The surface layer chitosan nano fiber is suitable for growth of cartilage cells, the basal layer micrometer chitosan support is suitable for the growth of the osteoblast, and the nano micrometer structure coexistence chitosan double-layer support can be used for restoring joint position of the human body cartilage and hard bone.

The invention discloses a silicosis treatment aerosol containing a human mesenchymal stem cell exosome extract and a preparation method thereof, wherein the main component of the aerosol is the high-concentration mesenchymal stem cell exosome extract. The mesenchymal stem cells can secrete various cell factors in the culture process, and the cell factors can penetrate into the basal layer of an inner membrane, promote tissue differentiation of the inner membrane, angiogenesis and growth of granulation tissues, promote regeneration and repair of damaged skin tissue structures, reduce generation of scar connective tissues and reduce bacterial infection and inflammatory reaction of wound surface tissues. According to the aerosol and the preparation method thereof, a method of continuous starvation of the mesenchymal stem cells is adopted, a high-concentration human mesenchymal extract can be obtained in a short period of time, and a big amount of stem cell exosomes can be separated from the mesenchymal extract by an exosome extractor. The aerosol containing the high-concentration mesenchymal stem cell exosome extract disclosed by the invention is suitable for the restoration and treatment of silicosis.

The invention relates to a periosteal repair sheet for guiding bone generation as well as a preparation method and an application of the periosteal repair sheet. The periosteal repair sheet is characterized by comprising a loose active layer high in porosity and a dense substrate layer low in porosity, wherein the loose active layer and the dense substrate layer comprise collage, polysaccharide substances and active factors; the loose active layer further comprises bone regeneration guiding active factors; and the periosteal repair sheet, when implanted into a body, can be degraded and absorbed. The repair sheet, which is of a dual-layer composite structure that the upper layer is loose and the lower layer is dense, is conducive to growth-in of cells and is capable of promoting repair speed of tissues; the dense layer is immunogen and the substrate layer has an isolating effect, so that the repaired effects can be effectively protected; and bone morphogenetic protein is added for periosteal injury and defect repair.

The invention belongs to the technical field of cytokines, and discloses a preparation method and application of human umbilical cord mesenchymal stem cell cytokines. The preparation method comprisesthe following steps: selecting easily obtained umbilical cord tissues, and separating stem cells in vitro and carrying out amplification culture to obtain stem cells with higher purity; then carryingout factory scale-up culture on the stem cells, collecting cell culture fluid, concentrating the culture fluid, and performing protein content determination, and quality inspection to obtain the cytokines. The cytokines prepared by the process of the invention contain the cytokines secreted by a plurality of stem cells; diluted cytokines can be injected into lyophilized powder, and further can bedirectly applied to affected parts or faces appropriately (used according to the indication of course of treatment), added to cosmetics proportionally, and used in the fields of beauty and skin repair, thereby being capable of repairing damaged skin cells of epidermis and a basal layer to achieve the effect of skin beauty.

A method for treating a patient suffering from a condition with an active compound comprising the steps of (a) treating the patient intranasally with an effective amount of MMP-9 or a functionally equivalent fragment, wherein the tight junctions of the patient's nasal epithelial cells are modulated or wherein the basal lamina of the patient is partially digested and type IV collagen of the patient is degraded or wherein access to the patient's perineural, perivascular, or lymphatic compartment spaces is facilitated and (b) treating the patient intranasally with an active compound is disclosed.

Wrinkles are cosmetically removed from a superficial area of mammalian skin tissue having an epidermal layer, a basal layer, and a dermal layer, by irradiating the dermal layer through the basal layer, the irradiation being selected to be absorbed by a chromphore in the dermal layer such that collagen present in the dermal layer is heated, while the basal layer remains intact so as to substantially inhibit contact of the dermal layer with ambient air.