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111 results about "Basal lamina" patented technology

The basal lamina is a layer of extracellular matrix secreted by the epithelial cells, on which the epithelium sits. It is often incorrectly referred to as the basement membrane, though it does constitute a portion of the basement membrane. The basal lamina is visible only with the electron microscope, where it appears as an electron-dense layer that is 20–100 nm thick (with some exceptions that are thicker, such as basal lamina in lung alveoli and renal glomeruli).

Placental tissue grafts and improved methods of preparing and using the same

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.
Owner:MIMEDX GROUP

Delivery of macromolecules into cells

An object of the invention is to provide a method for delivery of macromolecules into biological cells, such as Langerhans cells (22) in the epidermis (20) of a patient, which includes the steps of coating electodes (16) in an electrode assembly (12) with solid phase macromolecules to be delivered, such as a DNA, and / or RNA vaccine or a protein-based vaccine, attaching the electrode assembly (12) having the coated electrodes (16) to an electrode assembly holder (13), providing a waveform generator (15), establishing electrically conductive pathways between the electrodes (16), and the waveform generator (15), locating the electrodes (16) such that the biological cells are situated therebetween, such as by penetrating the needle electrode (16) into the epidermis (20) above the epidermal basal lamina, and providing pulse waveform from the waveform generator (15) to the electrodes (16), such that macromolecule on the electrodes (16) is driven off of the electrodes (16), and delivered into the biological cells, such as the Langerhans cells (22).
Owner:CELLECTIS SA

Multilayer microperforated implant

A multilayer microperforated implant comprising a plurality of microperforated substrate layers is provided. Methods of forming a microperforated implant comprise providing at least one substrate layer; perforating at least a region of the substrate layer; and dehydrating the substrate layer. Methods of augmenting a site in need of repair with the microperforated implant are also provided.
Owner:BIOMET SPORTS MEDICINE

Device, systems, methods and tools for continuous glucose monitoring

The present invention includes methods and apparatus for continuous glucose monitoring of a patient. In one aspect a glucose monitor includes a plurality of substantially cylindrical tissue piercing elements adapted to pierce the stratum corneum and enter the epidermis, allowing for the diffusion of glucose from the interstitial fluid into the glucose monitors described herein. In another aspect of the invention, a glucose monitor includes a deformed substrate layer defining a plurality of tissue piercing elements.
Owner:ARKAL MEDICAL

Placental tissue grafts and improved methods of preparing and using the same

Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.
Owner:MIMEDX GROUP +1

Multilayer microfluidic device

The present invention provides microfluidic devices constructed from four layers. The layers include a rigid substrate layer, a patterned rigid layer having thickness t, a patterned elastomeric layer having thickness greater than t, and a rigid support layer. Microfluidic structures in the devices are defined by the alignment of openings in the patterned rigid layer and the patterned elastomeric layer. The rigid support layer, rigid substrate layer, and patterned rigid layer may be made of any rigid material, including but not limited to plastic or silicon-containing materials, such as glass, quartz, or SiO2-coated materials. Similarly, the patterned elastomeric layer may be made of any elastomeric material, including but not limited to polydimethylsiloxanes, polymethylmethacrylates, perfluoropolyethers, or combinations thereof. Microfluidic devices according to the present invention may include sensors or sensor arrays. The microfluidic devices are fabricated using the provided error-tolerant alignment, biocompatible process.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV

Three dimensional vaginal tissue model containing immune cells

Disclosed is a cervico-vaginal tissue equivalent comprised of vaginal epithelial cells and immune cells, cultured at the air-liquid interface. The tissue equivalent is capable of being infected with a sexually transmitted pathogen such as a virus (e.g., HIV), a bacteria, a helminthic parasite, or a fungus. The tissue equivalent is also capable of undergoing an allergic-type reaction or an irritant-type reaction. The tissue equivalent is characterized as having nucleated basal layer cells and nucleated suprabasal layer cells, and further as having cell layers external to the suprabasal layer progressively increasing in glycogen content and progressively decreasing in nuclei content. Immune cells of the tissue equivalent are primarily located in the basal and suprabasal layers. Also disclosed are methods for producing the tissue equivalent. The methods involve providing vaginal epithelial cells and immune cells, seeding the cells onto a porous support, and co culturing the seeded cells at the air-liquid interface under conditions appropriate for differentiation. One such method disclosed is for generation of the tissue equivalent in serum free medium. Specific cells from which the tissue equivalent is generated, and also specific preferred components of the medium in which the tissue equivalent is generated are provided. Also disclosed is a cervico-vaginal tissue equivalent produced by the methods disclosed herein.
Owner:MATTEK CORP

Shifted Al-plating paint for offset printing, preparation method and application thereof

The invention relates to a shifted Al-plating paint for offset printing, a preparation method and application thereof. The paint provided by the invention comprises the following components of: by weight, 5-25 parts of cellulosic resin, 3-12 parts of polyacrylic resin, 0-5 parts of Vinyl chloride-acetate copolymer, 0-0.5 parts of an auxiliary agent and 70-86 parts of a solvent. The prepared shifted Al-plating paint can be used in the offset printing technology. In comparison with the prior art, the shifted Al-plating paint can be directly used for offset printing without the varnishing process, has excellent adhesive fastness with offset printing ink; the preparation method provided by the invention has advantages of simple technology and high production efficiency; the paint also has excellent peeling performance and good bonding fastness with an aluminium layer and a printing ink layer, and the PET basal lamina can be repeatedly used; in addition, the solvent used in the preparation of the paint contains no industrial forbidden solvent such as BTX, is safe and environmentally-friendly, and is beneficial for sustainable development.
Owner:SHANGHAI WEIKAI CHEM +1

Artificial blood vessel silk fibroin pre-coagulation coating

The invention discloses a soaking and coating method of artificial vessel silk protein, which is synthesized by woven or knitted elastic porous gap structure to coat at least five times through silk protein, wherein the false former of silk protein liquid vessel needs composite reinforcer, which is coated in the base layer evenly to expose in the formaldehyde steam after drying.
Owner:黄福华 +2

Preparation of black alumina substrate for multilayered wiring

The present invention discloses a method for preparing material of black alumina substrate used in multilayer wiring, namely adopts solid phase method, adds elements of Co, Mn, Cr, Si, Ti, B, V etc. into principal crystalline phase Al2O3 powder, modify oxide of alumina through adulterating, which recedes firing temperature, and brings out a black oxide of alumina organic doctor-blade casting method on the basis of modified powder, selects doctor-blade casting agent, double solvent and composite plasticizer that suitable for oxide of alumina; uniform and stable slurry with high solid content is obtained, bisque forming quality is enhanced; use doctor blade method for tape-casting, equipment simple and operational, natural drying process convenient, drying process will not lead rupture of billet body and phenomenon of unable stripping; obtained film body compact, and use mechanical drilling method to prepare stack basal lamina and obtain black alumina substrate used in multilayer wiring.
Owner:HUAZHONG UNIV OF SCI & TECH

Nail Strips Having a Crosslinked Polymer Top Coat

There is provided a nail enamel strip having a substrate layer and a nail enamel film with one or more nail enamel layers, at least one of the nail enamel layers having a cross-linked polymer. The nail enamel film is detachably adhered to the substrate layer. Further, there is provided a method for making the nail enamel strips, and a method of applying the nail enamel strip on a nail.
Owner:AVON PROD INC

Spiral microchannel polymerase chain reacting chip

A spiral microchannel polymerase chain reacting chip for preventive medicine and hygiene is disclosed. Basal layer with microflow-channel sealing with sealing cover coat by bonding technology, reagent sample inlet and outlet in cover coat, corresponding to channel origin and terminal, circular denaturating zone heater, circular extending zone heater and circular temperature sensor on another side of basal layer of microflow-channel, renaturating zone radiating zone with metal thin-membrane formation between insulating layer at right bottom of chip and basal layer with microflow-channel, insulating layer and metal wiring layer depositing on basal layer with microflow-channel. It can be used to improve compatibility, integration and efficiency of chip.
Owner:SHANGHAI JIAO TONG UNIV

Method and apparatus for diagnosing and correcting basal lamina offset

The invention discloses a substrate offset diagnosis and correction method, which comprises a diagnosis step (10) and a correction step (20) as follows: in the diagnosis step, determining whether the substrate carried by a transmission part is offset based on the detection result of a first sensor; in case of no offset, do not carry out the following correction step; in case of offset, carrying out the correction step; in the correction step, adjusting the location of the substrate, and then enabling the substrate to eliminate offset and return to the baseline position. In addition, the invention also provides a substrate offset diagnosis and correction device which comprises a diagnosis unit and a correction unit, wherein, the diagnosis unit is used to judge whether the substrate carried by the transmission part is offset; in case of offset, triggering the correction unit to work; in case of no offset, do not carry out any operation; the correction unit is used to adjust the location of the substrate and enable the substrate to eliminate offset and return to the baseline position. The method and the device are simple to use and enable the substrate to be accurately transmitted; besides, the device has high transmission accuracy and good reliability.
Owner:安徽尚特杰企业管理有限公司

Artificial blood vessel collagen pre-coagulation coating

The invention discloses a pre-coating method of artificial vessel collagen, which comprises the following steps: synthesizing vessel prosthese through woven or knitted elastic porous pipe structure, coating the collagen liquid with elasticizer for at least three times, setting the collagen in the base bottom evenly, drying to fix the collagen through heat-hinging method.
Owner:郑军 +2

Tissue engineering skin and preparation method thereof

ActiveCN104013999AQuick buildRestore skin functionProsthesisAnatomical structuresBasal lamina
The invention discloses tissue engineering skin which comprises seed cells and an acellular allogenic dermal matrix, wherein the seed cells and the acellular allogenic dermal matrix form a skin appendage; the seed cells are arranged, spread flat and printed at corresponding positions on the front side of the acellular allogenic dermal matrix according to an anatomical structure of normal skin by using a 3D (Three-dimensional) printer. The invention also discloses a method for preparing the tissue engineering skin. The invention provides perfect tissue engineering skin, and the tissue engineering skin can be rapidly constructed in a short time in an operating room (the operation is finished). Meanwhile, the tissue engineering skin comprises the seed cells and dermal matrix, the seed cells are basal layer cells, have the activities of multipotential stem cells and multipotential differentiation functions and are arranged and printed in the corresponding positions according to the anatomical structure of the normal skin, the skin appendage is easily formed, the cure rate is accelerated after the operation, and functions of the skin are restored.
Owner:广州市麦施缔医疗科技有限公司

Gas diffusion layer used for fuel cell with proton exchange film, production and application thereof

The invention relates to a gas diffusion layer for a proton exchange membrane fuel cell, wherein, the gas diffusion layer is composed of a basal cell layer and a microporous layer, wherein, the total weight percentage content of a hydrophobic agent in the basal cell layer is 1-50 percent; and the hydrophobic nature in the basal cell layer shows the gradient decrease from the side of the microporous layer to the side of a flow field. The preparation steps are as follows: a) the hydrophobic agent is evenly dispersed in a solvent to form slurry; b) the slurry is evenly prepared to the surface of a porous conductive basal cell layer material from one side, the slurry is sunk into the basal cell layer material under the action of gravity and viscous force, thereby realizing the gradient distribution in the basal cell layer; c) the basal cell layer of a gas diffusion electrode is formed by baking for 20-40 minutes at 300-400 DEG C. The gas diffusion layer is applied to hydrogen-oxygen proton exchange membrane fuel cells and direct alcohol fuel cells, as well as cells, electrolytic cells and sensors with the gas diffusion electrode structures. As the gas diffusion layer adopts the gradient hydrophobization, the gas diffusion layer is conductive to the discharge of water which is a cathode product and improves the cell performance.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Preparation method of nano micrometer structure coexistence chitosan double-layer support

The invention relates to a preparation method of a nano micrometer structure coexistence chitosan double-layer support, which belongs to the biology medical material field. The preparation method is characterized in that a phase separating technology is used to prepare a three-dimensional micrometer chitosan support as a basal layer, a high voltage static static spinning technology is used for spinning nano chitosan fiber on the micrometer chitosan surface, and thereby the nano micrometer structure coexistence chitosan double-layer support can be obtained. The prepared double layer support has good mechanical strength and high amount of porosity, and is suitable for adhesion, growth and differentiation of cells. The surface layer chitosan nano fiber is suitable for growth of cartilage cells, the basal layer micrometer chitosan support is suitable for the growth of the osteoblast, and the nano micrometer structure coexistence chitosan double-layer support can be used for restoring joint position of the human body cartilage and hard bone.
Owner:QINGDAO UNIV

Silicosis treatment aerosol containing human mesenchymal stem cell exosome extract and preparation method thereof

The invention discloses a silicosis treatment aerosol containing a human mesenchymal stem cell exosome extract and a preparation method thereof, wherein the main component of the aerosol is the high-concentration mesenchymal stem cell exosome extract. The mesenchymal stem cells can secrete various cell factors in the culture process, and the cell factors can penetrate into the basal layer of an inner membrane, promote tissue differentiation of the inner membrane, angiogenesis and growth of granulation tissues, promote regeneration and repair of damaged skin tissue structures, reduce generation of scar connective tissues and reduce bacterial infection and inflammatory reaction of wound surface tissues. According to the aerosol and the preparation method thereof, a method of continuous starvation of the mesenchymal stem cells is adopted, a high-concentration human mesenchymal extract can be obtained in a short period of time, and a big amount of stem cell exosomes can be separated from the mesenchymal extract by an exosome extractor. The aerosol containing the high-concentration mesenchymal stem cell exosome extract disclosed by the invention is suitable for the restoration and treatment of silicosis.
Owner:TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD

Periosteal repair sheet for guiding bone generation as well as preparation method and application of periosteal repair sheet

The invention relates to a periosteal repair sheet for guiding bone generation as well as a preparation method and an application of the periosteal repair sheet. The periosteal repair sheet is characterized by comprising a loose active layer high in porosity and a dense substrate layer low in porosity, wherein the loose active layer and the dense substrate layer comprise collage, polysaccharide substances and active factors; the loose active layer further comprises bone regeneration guiding active factors; and the periosteal repair sheet, when implanted into a body, can be degraded and absorbed. The repair sheet, which is of a dual-layer composite structure that the upper layer is loose and the lower layer is dense, is conducive to growth-in of cells and is capable of promoting repair speed of tissues; the dense layer is immunogen and the substrate layer has an isolating effect, so that the repaired effects can be effectively protected; and bone morphogenetic protein is added for periosteal injury and defect repair.
Owner:BEIJING BIOSIS HEALING BIOLOGICAL TECH

Artificial blood vessel silk fibroin and collagen blending pre-coagulation coating

The invention discloses a stratified pre-condensing coating method of artificial vessel collagen and silk protein, which comprises the following steps: synthesizing vessel prosthesis through woven or knitted elastic porous pipe structure, using three-layered coating technology to coat collagen and reinforcer liquid on the internal wall of vessel prosthesis. The vessel prosthesis of silk protein liquid vessel needs composite reinforcer, which is coated in the base layer evenly to expose in the formaldehyde steam after drying.
Owner:郑军 +2

Preparation method and application of human umbilical cord mesenchymal stem cell cytokines

The invention belongs to the technical field of cytokines, and discloses a preparation method and application of human umbilical cord mesenchymal stem cell cytokines. The preparation method comprisesthe following steps: selecting easily obtained umbilical cord tissues, and separating stem cells in vitro and carrying out amplification culture to obtain stem cells with higher purity; then carryingout factory scale-up culture on the stem cells, collecting cell culture fluid, concentrating the culture fluid, and performing protein content determination, and quality inspection to obtain the cytokines. The cytokines prepared by the process of the invention contain the cytokines secreted by a plurality of stem cells; diluted cytokines can be injected into lyophilized powder, and further can bedirectly applied to affected parts or faces appropriately (used according to the indication of course of treatment), added to cosmetics proportionally, and used in the fields of beauty and skin repair, thereby being capable of repairing damaged skin cells of epidermis and a basal layer to achieve the effect of skin beauty.
Owner:西安东澳生物科技有限公司

Methods and Compositions for Enhancing Intranasal Delivery of Therapeutic Agents

A method for treating a patient suffering from a condition with an active compound comprising the steps of (a) treating the patient intranasally with an effective amount of MMP-9 or a functionally equivalent fragment, wherein the tight junctions of the patient's nasal epithelial cells are modulated or wherein the basal lamina of the patient is partially digested and type IV collagen of the patient is degraded or wherein access to the patient's perineural, perivascular, or lymphatic compartment spaces is facilitated and (b) treating the patient intranasally with an active compound is disclosed.
Owner:WISCONSIN ALUMNI RES FOUND

Tension-free hernia patch and preparation method thereof

Belonging to the field of organic polymer compound preparation or chemical processing, the invention provides a tension-free hernia patch and a preparation method thereof. The patch provided in the invention has good toughness and strength match, no adverse reaction in the human body, and excellent biocompatibility, and is free of inflammation and foreign body reaction. When the patch is implanted into organisms, abdominal adhesions and intestinal fistulas are greatly reduced, so that the patch is a good material for incisional hernias, abdominal hernias, stomal hernias, umbilical hernias and other herniorrhaphies. The patch includes a basal layer made of a non-absorbable material and a surface coating made of an absorbable material. The surface coating is softer, and can be completely absorbed by the human body in 60 days, thus providing time for combination of the basal layer and surrounding tissues. The patch provided in the invention has a simple preparation process and a low manufacturing cost. As a medical tension-free hernia patch, it has excellent comprehensive performance, and is a safe and reliable patch, thus having great social and economic value.
Owner:王明刚 +1

Apparatus for wrinkle removal

Wrinkles are cosmetically removed from a superficial area of mammalian skin tissue having an epidermal layer, a basal layer, and a dermal layer, by irradiating the dermal layer through the basal layer, the irradiation being selected to be absorbed by a chromphore in the dermal layer such that collagen present in the dermal layer is heated, while the basal layer remains intact so as to substantially inhibit contact of the dermal layer with ambient air.
Owner:ICN PHOTONICS

Visual rapid combined measuring method of plant pathogen and antibody chip

The invention discloses a visual rapid combined measuring method of plant pathogen and an antibody chip. The method provided by the invention comprises the following steps of: distributing plant pathogen grabbing antibody points on basal lamina to obtain an antibody chip, hybridizing a detected sample and the antibody chip for incubation,washing, adding a corresponding plant pathogen detection antibody, continuously reacting, washing and developing. The method provided by the invention has the same sensitivity, specificity and reproducibility with methods such as ELISA and the like, and also has the capability of detecting a plurality of pathogens in one experiment, which is what traditional methods don't have.
Owner:上海慧耘生物科技有限公司
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