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Preparation method and application of human umbilical cord mesenchymal stem cell cytokines

A cytokine and stem cell technology, applied in animal cells, vertebrate cells, preparations for skin care, etc., can solve problems such as inability to remove toxins, and achieve the effect of restoring skin youthfulness, improving skin tone, and improving effects.

Inactive Publication Date: 2019-03-15
西安东澳生物科技有限公司
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  • Application Information

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Problems solved by technology

In this method, the cells have been lysed and died. After the cytokines are collected, the cells can no longer be subcultured, and the toxins released by cell lysis cannot be removed.

Method used

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  • Preparation method and application of human umbilical cord mesenchymal stem cell cytokines
  • Preparation method and application of human umbilical cord mesenchymal stem cell cytokines
  • Preparation method and application of human umbilical cord mesenchymal stem cell cytokines

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preparation example Construction

[0050] Such as figure 1 As shown, the preparation method of human umbilical cord mesenchymal stem cell cytokines provided by the embodiments of the present invention, the steps are as follows:

[0051] Step 1: Primary isolation and culture of human umbilical cord mesenchymal stem cells

[0052] 1) Place the received umbilical cord tissue that has passed the clinical test into a sterile collection bottle containing 50-100ml of normal saline, and keep it sealed. Use an incubator (transported with ice packs) and return to the sterile laboratory.

[0053] 2) In the ultra-clean workbench, take out the umbilical cord from the collection bottle, put it into a petri dish, cut the umbilical cord into 3-4cm long pieces with long-handled surgical scissors, and wash it repeatedly with normal saline (containing 1% double antibody) for 3 -5 times, remove blood stains and achieve clarity.

[0054] 3) Use ophthalmic curved scissors to cut the umbilical cord along the spiral trend of the v...

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Abstract

The invention belongs to the technical field of cytokines, and discloses a preparation method and application of human umbilical cord mesenchymal stem cell cytokines. The preparation method comprisesthe following steps: selecting easily obtained umbilical cord tissues, and separating stem cells in vitro and carrying out amplification culture to obtain stem cells with higher purity; then carryingout factory scale-up culture on the stem cells, collecting cell culture fluid, concentrating the culture fluid, and performing protein content determination, and quality inspection to obtain the cytokines. The cytokines prepared by the process of the invention contain the cytokines secreted by a plurality of stem cells; diluted cytokines can be injected into lyophilized powder, and further can bedirectly applied to affected parts or faces appropriately (used according to the indication of course of treatment), added to cosmetics proportionally, and used in the fields of beauty and skin repair, thereby being capable of repairing damaged skin cells of epidermis and a basal layer to achieve the effect of skin beauty.

Description

technical field [0001] The invention belongs to the technical field of cytokines, in particular to a preparation method and application of human umbilical cord mesenchymal stem cell cytokines. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Stem cells are cells with multidirectional differentiation potential and unlimited self-renewal and replication capabilities. Under certain conditions, they can differentiate into specific tissues. During the process of growth, proliferation, and differentiation, stem cells secrete extracellular active substances such as cytokines that promote cell growth and regulate the surrounding environment. [0004] Mesenchymal stem cells have the ability of self-replication and multiple differentiation potentials. They can differentiate into functional cells in vivo or under specific conditions. During the process of proliferation and differentiation, they secrete a variet...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12P21/00A61K8/64A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/64A61Q19/00A61Q19/02A61Q19/08C12N5/0665C12P21/00
Inventor 晁晓东王泊
Owner 西安东澳生物科技有限公司
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