Periosteal repair sheet for guiding bone generation as well as preparation method and application of periosteal repair sheet

Abstract

The invention relates to a periosteal repair sheet for guiding bone generation as well as a preparation method and an application of the periosteal repair sheet. The periosteal repair sheet is characterized by comprising a loose active layer high in porosity and a dense substrate layer low in porosity, wherein the loose active layer and the dense substrate layer comprise collage, polysaccharide substances and active factors; the loose active layer further comprises bone regeneration guiding active factors; and the periosteal repair sheet, when implanted into a body, can be degraded and absorbed. The repair sheet, which is of a dual-layer composite structure that the upper layer is loose and the lower layer is dense, is conducive to growth-in of cells and is capable of promoting repair speed of tissues; the dense layer is immunogen and the substrate layer has an isolating effect, so that the repaired effects can be effectively protected; and bone morphogenetic protein is added for periosteal injury and defect repair.

Description

technical field [0001] The invention relates to the field of medical biomaterials, specifically a periosteal patch for repairing periosteal damage or defect caused by trauma, fracture, tumor, etc., or implanting with bone graft materials to prevent material prolapse, a preparation method and an application. Background technique [0002] Fractures, nonunions, delayed unions, and bone defects caused by aging, trauma, and tumors are common orthopedic diseases in clinical practice. The treatment research focuses on bone graft materials, while ignoring the importance of periosteum repair. In fact, the periosteum is rich in microvessels that can restore blood supply to bone tissue, thereby promoting the healing of bone defects and increasing the success rate of bone repair; and it can serve as an important interface between bone tissue and surrounding tissues, preventing muscle, tendon, tendon Membrane and other tissues grow into the bone defect, providing a growth microenvironmen...

AI Technical Summary

Problems solved by technology

Therefore, once the periosteum loss cannot be restored, the bone defect will be difficult to heal itself

[0003] The currently reported periosteum repair materials have deficiencies. The use of acellular matrix materials has a large matrix damage, high DNA residues, incomplete removal of immunogenicity, and easy to cause immune rejection; chitosan, silk fibroin and other natural Most biomaterials use electrospinning technology to construct three-dimensional structures, which cannot achieve the porous network effect of natural extracellular matrix; artificially synthesized degradable polymer materials will cause local acidity during the degradation process, which can easily cause inflammation; and collagen The protein film has insufficient mechanical strength and degrades too quickly. The use of chemical cross-linking agents will cause tissue infection, inflammation, and fiber wrapping; or growth factors such as BMP, VEGF, and bFGF need to be added to the matrix to improve the ability of the material to induce tissue regeneration.

Therefore, there is still no good periosteum repair material, which can not only play a barrier role, but also effectively isolate the bone injury site from the surrounding tissue, prevent the fibroblasts in the surrounding tissue from invading the bone injury site and the prolapse of the bone graft material, and can Guide the regeneration and repair of periosteal tissue, form vascularized tissue, provide nutrients and oxygen for bone regeneration, and provide a growth microenvironment for osteoblasts and mesenchymal stem cells to rebuild bone tissue

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