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Artificial blood vessel silk fibroin pre-coagulation coating

A technology of artificial blood vessels and silk fibroin, applied in coatings, medical science, prostheses, etc., can solve problems such as artificial blood vessels cannot be implanted, difficult to obtain, and unstable coating materials

Inactive Publication Date: 2007-02-28
黄福华 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the above-mentioned coating materials are unstable, difficult to obtain, and expensive (J.H.Lee, W.G.Kim, and S.S.Kim, "Development and characterization of an alginate impregnated polyester vascular graft," J Biomed Mater Res 36, 200-208, (1997 )), while using bovine collagen, gelatin as the coating material of the artificial blood vessel can not be implanted in patients allergic to the bovine Brucella antigen

Method used

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  • Artificial blood vessel silk fibroin pre-coagulation coating
  • Artificial blood vessel silk fibroin pre-coagulation coating
  • Artificial blood vessel silk fibroin pre-coagulation coating

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Example 1: silk fibroin extraction method

[0022] Tussah silk in 0.5% (by weight) Na 2 CO 3 Boil in the solution for 1-1.5h to remove sericin (silk weight (g) and Na 2 CO 3 The solution volume (ml) ratio is between 1 / 80-1 / 67), using distilled water to repeatedly wash the silk stripped of sericin until it is completely clean. Put the clean silk from sericin into CaCl 2 / H 2 O / C 2 h 5 OH=1: 8: 2 (molar ratio) in the solvent (take off the silk weight (g) of sericin and H 2 O, C 2 h 5 OH volume (ml) and ratio between 1 / 10-1 / 15, then deduce CaCl 2 quality), dissolved at 70-80°C for 30-45 minutes, cooled and put into a dialysis bag, dialyzed with distilled water for 3 days, filtered to obtain a high-purity silk fibroin aqueous solution, and then concentrated the silk fibroin solution by heating and magnetic stirring to reach Prepare the concentration of the coating material (the temperature of the magnetic stirrer is controlled at 45-55° C.).

example 2

[0023] Example 2: Artificial blood vessel coated with silk fibroin solution

[0024]Containing silk fibroin (purified from tussah silk according to example 1) concentration is that 12% silk fibroin mixed solution is filled in the syringe of 50ml, and this silk fibroin mixed solution contains 10% glycerin simultaneously, 18% ethanol and distilled water , with a viscosity of about 28,000 centipoise. The syringe is connected to one end of the synthetic woven artificial blood vessel, and the silk fibroin mixture is pushed into the lumen of the artificial blood vessel so that the coating liquid is evenly coated on the inner wall of the lumen, and the excess silk fibroin mixture is removed from the other end of the artificial blood vessel. The artificial blood vessel after coating is dried at room temperature for 1 / 2 hour, and the coating drying operation is repeated at least 5 times.

[0025] After the fifth coating, the artificial blood vessel was exposed to formaldehyde vapor fo...

example 3

[0026] Example 3: Comparison of blood permeability of artificial vascular prosthesis before and after coating

[0027] According to the method of example 2, the artificial blood vessel of the silk fibroin solution coating that does not leak blood is prepared and tested as follows, 5 synthetic woven artificial blood vessels with a diameter of 8 mm and a length of 12 cm are randomly selected and connected to the blood storage device, and the artificial blood vessel is tested at 120 mmHg Under pressure, the heparinized blood is passed through the blood vessel, and the leaked blood is collected and measured (unit: ml / min*cm 2 ). Test results The blood exudation rates of the five artificial blood vessels are: 0.05, 0.0, 0.03, 0.04 and 0.0, with an average blood exudation rate of 0.023ml / min*cm 2 , close to zero.

[0028] In order to compare with the uncoated woven artificial blood vessel, the uncoated artificial blood vessel was selected to repeat the above experiment, and the av...

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Abstract

The invention discloses a soaking and coating method of artificial vessel silk protein, which is synthesized by woven or knitted elastic porous gap structure to coat at least five times through silk protein, wherein the false former of silk protein liquid vessel needs composite reinforcer, which is coated in the base layer evenly to expose in the formaldehyde steam after drying.

Description

Technical field [0001] The present invention relates to a pre-coagulated coating method for artificial vascular prosthesis that can be used for replacement, bypass grafting or shunting of any part of human vascular system, especially the use of silk fibroin solution mixed with plasticizer to coat artificial vascular prosthesis , so that it does not leak blood so as to avoid the method of pre-coagulation operation during the operation. Background technique [0002] Replacement of human diseased blood vessels with artificial vascular prosthesis is a mature surgical technique. Vascular grafts come in many different types and can be made of different materials. Artificial vascular prostheses that can be implanted into the human body are usually made of biocompatible materials, and at the same time, the vascular prosthesis can maintain an open tubular structure and allow blood flow to run unimpeded after implantation. Vascular prostheses can be constructed of biocompatible fibr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/56
Inventor 黄福华郑军孙立忠
Owner 黄福华
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