Spiral microchannel polymerase chain reacting chip

A technology of chain reaction and polymerase, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, fermentation, etc. It can solve the problems of unreasonable layout of microchannels in temperature zone design, and improve compatibility and integration , expanding the scope of use, and the effect of reasonable distribution of hot spots

Inactive Publication Date: 2005-02-23
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to propose a spiral microchannel polymerase chain reaction chip for the defects of insufficient temperature zone design and unreasonable microchannel layout of the existing polymerase chain reaction chip. The layout of the area makes the temperature change experienced by the reagents flow on the chip conform to the amplification curve of the polymerase chain reaction, thereby improving the yield of amplification and solving the problems in the background technology

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  • Spiral microchannel polymerase chain reacting chip
  • Spiral microchannel polymerase chain reacting chip
  • Spiral microchannel polymerase chain reacting chip

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Embodiment Construction

[0018] As shown in Figures 1, 2, 3, 4, and 5, the present invention includes: a sealing cover layer 1, a base layer 2 with a microfluidic channel, a spiral microfluidic channel 4, a reagent sample output port 6, and a reagent sample input port 7 , an insulating layer 12 and a metal lead layer 13, an annular temperature sensor 18, a heat dissipation area 19 in an annealing zone, a heater 21 in an annular denaturation zone, and a heater 22 in an annular extension zone. The microfluidic channel on the top is a spiral microfluidic channel 4, the base layer 2 with the microfluidic channel is packaged with the sealing cover layer 1, and the sealing cover layer 1 is provided with a reagent sample input port 7 and a reagent sample output port 6. The sample input port 7 and the reagent sample output port 6 correspond to the channel start point 14 and the channel terminal point 16 respectively, and the other side of the base layer 2 with the microfluidic channel is provided with an annul...

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Abstract

A spiral microchannel polymerase chain reacting chip for preventive medicine and hygiene is disclosed. Basal layer with microflow-channel sealing with sealing cover coat by bonding technology, reagent sample inlet and outlet in cover coat, corresponding to channel origin and terminal, circular denaturating zone heater, circular extending zone heater and circular temperature sensor on another side of basal layer of microflow-channel, renaturating zone radiating zone with metal thin-membrane formation between insulating layer at right bottom of chip and basal layer with microflow-channel, insulating layer and metal wiring layer depositing on basal layer with microflow-channel. It can be used to improve compatibility, integration and efficiency of chip.

Description

technical field [0001] The invention relates to a polymerase chain reaction chip, in particular to a spiral microchannel polymerase chain reaction chip. For use in preventive medicine and hygiene. Background technique [0002] In the chemical analysis system of molecular biology, the polymerase chain reaction is a crucial component, and the development of micro-electromechanical technology (MEMS) enables the polymerase chain reaction to be completed on the chip. The general microchannel polymerase chain reaction chip uses three temperature zones to control the three reaction steps of denaturation, renaturation and extension respectively. The microfluidic channel made on the chip circulates through the repeated changes of these three temperature zones, so In the mixed reagents participating in the reaction in the channel, the fragments of deoxyribonucleic acid (DNA) molecules are amplified in geometric progression when they flow in the microfluidic channel. The microchannel...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/34C12Q1/68
Inventor 陈文元贾晓宇牛志强张卫平
Owner SHANGHAI JIAO TONG UNIV
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