Preparation method of anti-infectious lower biomaterial of small intestinal mucosa

A technology of small intestinal submucosa and biomaterials, applied in medical science, prosthesis, etc., to achieve the effect of enhancing digestion

Inactive Publication Date: 2019-02-15
陈德夫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the source of the anti-infective component of the patent compound is limited to silver nitrate solution

Method used

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  • Preparation method of anti-infectious lower biomaterial of small intestinal mucosa
  • Preparation method of anti-infectious lower biomaterial of small intestinal mucosa
  • Preparation method of anti-infectious lower biomaterial of small intestinal mucosa

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preparation example Construction

[0059] The invention provides a method for preparing an anti-infection small intestinal submucosa biological material, comprising the following steps:

[0060] 1) Initial treatment: take the small intestine of the pig within 5 hours after slaughter, cut it into sections, each section is 3-30 cm long, then cut it into slices along the central axis, and rinse it repeatedly with clean water (no requirement, subjective judgment is enough) ), using mechanical methods to remove the mucosal layer, muscular layer and serosal layer to obtain submucosal tissue SIS, and then rinse with water for 5-10 times;

[0061] 2) Degreasing: The submucosa tissue obtained in step 1) is degreased with a degreasing agent for 30-90 minutes, and after degreasing, rinse with water for 5-10 times.

[0062] In the above step 2), the mixed degreasing agent can be composed of a trisodium phosphate solution with a mass concentration of 1-3% and a surfactant with a mass concentration of 0.1-1.0%; the trisodium...

Embodiment 1

[0086] This embodiment provides a method for preparing an anti-infection SIS biomaterial, comprising the following steps:

[0087] Primary treatment: take the small intestine of the pig within 5 hours after slaughter, cut it into small sections with a length of 8 cm, cut it longitudinally into slices, wash it repeatedly with clean water, remove the mucosal layer, muscular layer and serosa layer by mechanical means, and obtain the submucosa tissue of the small intestine SIS; rinse with water 5 times, 20 minutes each.

[0088] Degreasing: use a mixed degreasing agent consisting of 1% sodium bicarbonate solution, 3.5% sodium hydroxide solution and 0.5% Triton X-100 for 30 minutes. After degreasing, rinse with water 10 times, 3 minutes each time. The mass ratio of SIS to mixed degreasing agent is 1:5.

[0089] Virus inactivation: use a virus inactivation solution composed of tributyl phosphate solution with a mass concentration of 0.3% and Tween 80 with a mass concentration of 2...

Embodiment 2

[0097] This embodiment provides a method for preparing an anti-infection SIS biomaterial, comprising the following steps:

[0098] Initial treatment: take pig small intestine within 5 hours after slaughter, cut it into 6 cm long pieces, cut it longitudinally into slices, wash it repeatedly with clean water, remove the mucosal layer, muscular layer and serosal layer by mechanical means, and obtain the submucosa tissue SIS ; Rinse with water 10 times, 10 minutes each time.

[0099] Degreasing: using a mixed degreasing agent consisting of 5% sodium bicarbonate solution, 1.5% sodium hydroxide solution and 1.0% Triton X-100 for 90 minutes. After degreasing, rinse with water 5 times, 8 minutes each time. The mass ratio of SIS to mixed degreasing agent is 1:10.

[0100]Virus inactivation: use a virus inactivation solution composed of tributyl phosphate solution with a mass concentration of 0.5% and sodium cholate with a mass concentration of 0.5%, soak for 10 hours to inactivate th...

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Abstract

The invention relates to a preparation method of an anti-infectious lower biomaterial of small intestinal mucosa. The method comprises the following steps: primarily treating; degreasing; inactivatingvirus; decellularizing; loading antibacterial components; forming; freezing drying or soaking in a storing solution; packing; sterilizing. The lower biomaterial prepared according to the method is good in decellularizing effect; the damage to the structure of a raw material is reduced; an SIS material subjected to decellularizing is complete and uniform in a collagenous fiber structure by observing through a light microscope; the collagenous fiber beam is complete; extremely few cells are residual; complete cells cannot be observed; the collagenous fibers of the material are of three-dimensional net shaped structures by being observed under a scanning electron microscope, and cells are not observed, and few broken cell pieces are residual; after the antibacterial components are loaded tothe SIS, the antibacterial effect is obvious; the clinical demand can be met.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method of an anti-infection small intestinal submucosa biological material. Background technique [0002] The small intestinal submucosa (SIS) biomaterial is a natural extracellular matrix (ECM) tissue repair material, which can guide and support the growth of host cells in vivo, and finally gradually degrades completely, and the regenerated tissue structure is remodeled take shape. SIS can be used for the repair of tissue defects such as nerves, bladder, blood vessels, tendons, and abdominal wall, and as a wound dressing to promote wound healing and repair. SIS has unique advantages, especially for infected wounds, SIS has an effect that other artificial polymer repair materials cannot match. However, for severely infected wounds, relying solely on the anti-infective activity of SIS is not enough, and it is necessary to use it in combination with local antibac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54
CPCA61L27/3629A61L27/3687A61L27/3691A61L27/54A61L2300/404A61L2300/406A61L2300/604
Inventor 陈德夫
Owner 陈德夫
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