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Placenta tissue matrix material and preparation method thereof

A technology of placental tissue and matrix materials, applied in the field of medical biomaterials and human tissue engineering, can solve the problems of mismatch between degradation rate and tissue reconstruction, poor integration of tissue engineering materials and body tissues, etc., to accelerate repair and support cell migration Effect

Active Publication Date: 2020-04-10
银丰低温医学科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the above-mentioned prior art, aiming at the limitations of current tissue engineering materials such as poor integration with body tissues, and mismatch between degradation rate and tissue reconstruction, the present invention provides a medical biomaterial placental tissue matrix material prepared from human placental tissue

Method used

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  • Placenta tissue matrix material and preparation method thereof
  • Placenta tissue matrix material and preparation method thereof
  • Placenta tissue matrix material and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] The pretreatment of embodiment 1 placental tissue

[0051] Proceed as follows:

[0052] Human placental tissue (including the attached umbilical cord and amniotic membrane tissue) collected from the hospital, if there is no special protection measures, the cells in the tissue will soon start to undergo apoptosis, and various proteolytic enzymes are released during the cell death process, which may cause extracellular damage to the tissue The matrix has adverse effects; the placenta and its subsidiary tissues are rich in nutrients, and microorganisms are easy to breed during transshipment, increasing the risk of product contamination. In addition, if the collected placental tissue cannot be used for extracellular matrix preparation immediately, cryopreservation is required, and cryopreservation will also damage the placental tissue. Therefore, the collected placental tissue needs to be pretreated immediately to ensure the quality of the tissue raw material.

[0053] (1...

Embodiment 2

[0065] The preparation of embodiment 2 placental decellularized matrix gel

[0066] Proceed as follows:

[0067] (1) Tissue pretreatment: take fresh placental tissue, cut into tissue blocks (0.2-2 cm in length, width and height), wash (with saline) (to remove blood stains), and then place in EDTA-containing PBS buffer (EDTA Concentration of 5mM), washing by shaking on a shaker (washing by shaking for 4 hours, rotating speed 100rpm / min); homogenate treatment after shaking and washing, intermittent crushing (10000rpm / min, 10s×3 times), to obtain a homogenate, use Properly dilute with EDTA-containing PBS buffer (the concentration of EDTA is 5mM), dispense into centrifuge tubes, and centrifuge (1000×g, 10min) to obtain tissue pellets; repeat shaking and washing 3 times to obtain tissue granules;

[0068] (2) Sterilization and virus inactivation: place the tissue particles in a solution for sterilization and virus inactivation (continuous shaking on a shaker at room temperature fo...

Embodiment 3

[0091] Example 3 Preparation of placental acellular matrix sponge

[0092] The steps are as follows: the matrix gel obtained in Example 2 was left to stand for 2 hours to refibrillate the hydrogel placental tissue matrix, and then freeze-dried to obtain the acellular matrix sponge. The specific program of freeze-drying is: cool down to -40°C at a rate of 0.1-0.5°C per minute, pre-cool at -40°C for 2 hours, start the vacuum, raise the temperature of the sample rack to -15°C, and the pressure of the freeze-dryer box adjusted to 40Pa, and freeze-dried for 25 hours to obtain the decellularized matrix sponge.

[0093] Quality evaluation of placental acellular matrix sponge:

[0094] (1) Paraffin-embedded the decellularized placental matrix and normal placental tissue respectively, and the sections were stained with HE. No obvious blue-stained nuclei were observed in the decellularized placental tissue under a microscope, see Figure 6 .

[0095] (2) The placental acellular matri...

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Abstract

The invention discloses a placenta tissue matrix material. A placenta extracellular matrix is prepared by taking placenta tissue as a raw material through the technologies of tissue preservation, tissue treatment, sterilization, virus inactivation and the like, the placenta extracellular matrix retains a natural matrix structure and basic biological characteristics, and the extracellular matrix particularly retains receptors of various active factors. A preparation method comprises the following steps: tissue pretreatment; sterilization and virus inactivation; tissue DNA removal; decellularization; and preparation of various forms such as gel, sponge or powder from the acellular matrix according to specific clinical requirements. The acellular matrix disclosed by the invention is a specialscaffold material with an active factor receptor, can actively attract active ingredients in a living body into the material, is beneficial to accumulation of cell active ingredients, supports cell migration, adhesion, proliferation and differentiation, and can accelerate damaged tissue repair and local tissue reconstruction.

Description

technical field [0001] The invention relates to a placenta tissue matrix material and a preparation method thereof, belonging to the field of medical biological materials and human tissue engineering. Background technique [0002] The repair and reconstruction of tissues and organs has always been a research hotspot in the fields of tissue engineering, biology, and medicine. With the development of technology, tissue engineering products have gradually become clinically important surgical repairs and substitutes. It is an important treatment measure for the loss of human tissues and organs and various injuries. Tissue engineering materials are the basis for the research and development of this product, which can be used independently or in combination with stem cell seeds to become the first choice for clinical surgical repair and replacement. [0003] At present, most tissue engineering materials use artificially synthesized and natural polymer materials as the main compon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/54A61L27/56A61L27/58
CPCA61L27/58A61L27/3633A61L27/3687A61L27/3691A61L27/54A61L27/56
Inventor 刘琇臧传宝刘峰李夏
Owner 银丰低温医学科技有限公司
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