Extending tissue preservation

a tissue preservation and tissue technology, applied in the field of tissue preservation for transplantation, can solve the problem that the demand for corneal transplantation exceeds the available supply of corneal cells

Inactive Publication Date: 2002-08-01
MANGAT HARPAL S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Demand for corneal transplantation ex

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  • Extending tissue preservation
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example 1

[0052] Seven white New Zealand rabbits weighing two-three kg were killed with sodium pentobarbital overdose. Their eyes were flooded with neosporin. Corneas were excised from both eyes within ten minutes of termination. The right cornea were placed in a mixture of oxygenated perfluorocarbon emulsion and Optisol. The left eyes were placed in pure Optisol. In both cases the corneas were immersed in the Optisol layer. All corneas were stored at four degrees centigrade for 20 days. The corneal thickness was measured using the Dickstein-Maurice technique with a pre-calibrated specular microscope. Central cornea measurements were on days 0, 6, 12 and 18 (average of three measurements). The oxygen tension was measured using a Yellow Spring oxygen electrode. Care was taken to ensure the reading was done at the level of the cornea. Results are the average of readings taken at the 4, 8 and 12 o'clock position on days 0, 6, 12 and 18. On day 21, lacticacid was measured as follows. The corneas ...

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Abstract

A method of preserving tissue by placing the tissue in a media containing pure perfluorocarbon is provided. Also provided is a tissue preserving media including storage media and pure perfluorocarbon. A method of making media by adding pure perfluorocarbon to the media is provided. A method of preserving tissue by oxygenating the storage media by adding pure perfluorocarbons to the media is also provided.

Description

CROSSREFERENCE TO RELATED APPLICATIONS[0001] This application is based on a Provisional Application, Serial No. 60 / 073,202 filed Jan. 30, 1998.GOVERNMENT SUPPORT[0002] Not Applicable[0003] 1. Technical Field[0004] The present invention relates to the field of preservation of tissue for transplantation and more specifically to storage media that can extend tissue preservation.[0005] 2. Description of the Related Art[0006] Corneal transplantation is the most common form of organ transplantation practiced in the United States. Two procedures for transplantation are utilized. Penetrating keratoplasty is used in about 90% of the cases, with lamellar keratoplasty being utilized in the remaining situations. Demand for corneal transplantation exceeds the available supply of corneas. Advances in procurement and preservation must continue to meet this demand. JAMA 1998 February 5:259(5):719-22.[0007] The need for corneal transplantation in the United States stem from conditions which effect t...

Claims

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Application Information

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IPC IPC(8): A01N1/02A61K31/025A61K31/13A61K31/445A61K31/47A61K31/755C12N5/02
CPCA01N1/02A01N1/0226A61K31/025A61K31/13A61K31/445A61K31/47A61K31/755
Inventor MANGAT, HARPAL S.
Owner MANGAT HARPAL S
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