Novel cadaver specimen preserving fluid

A technology for preserving solutions and specimens, which is applied in the fields of medicine and teaching, and can solve the problems of high cost of glycerin, short maintenance time of effective concentration, and unsuitability for large specimen preservation.

Active Publication Date: 2015-04-29
CENT SOUTH UNIV +1
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Problems solved by technology

2. The specimen preservation solution should not have a strong dehydration effect
Existing preservation methods cannot well meet the above-mentioned requirements, for example: 1. Add an appropriate amount of ammonia water to the formaldehyde solution. Although this method reduces the pungent smell of formaldehyde, the volatilization and toxicity of formaldehyde still exist
2. Use 70% alcohol instead of 5% formalin. However, alcohol...

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  • Novel cadaver specimen preserving fluid
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Embodiment Construction

[0008] The following in conjunction with specific embodiments is intended to further illustrate the present invention, rather than limit the present invention.

[0009] According to the raw material composition determined in the present invention, phenoxyethanol is first dissolved in distilled water, and then potassium sorbate, vitamin C, thymelic acid, sodium chloride and ferric chloride are added respectively.

[0010] The inventor uses formalin (10% formaldehyde) solution (A 1 Liquid) and the antiseptic perfusate of the present invention (B 1 solution) to fix myocardial tissue and skeletal muscle tissue, and after one and a half years, observe the structural changes of histomorphology under a light microscope, and conduct a comparative study. The results are shown in the attached figure 1 and Table 1.

[0011] Table 1 Comparison results of tissue structure under light microscope

[0012]

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Abstract

The invention belongs to medical anatomy, and particularly relates to a novel cadaver specimen preserving fluid. The novel cadaver specimen preserving fluid comprises the following components in parts by mass: 0.6-6 parts of phenoxyethanol, 0.6-6 parts of potassium sorbate, 0.3-1 part of vitamin C, 0.1-0.6 part of musk oxalic acid, 0.6-1.5 parts of sodium chloride, 0.6-1.5 parts of ferric chloride and 78-97 parts of water. The novel cadaver specimen preserving fluid is good in preservation effect, and reliable in antiseptic and anti-mold properties on the cadaver specimen; the specimen does not mould, does not shrink, is natural in color, and flexible and moist in tissue; and the preserving fluid is non-toxic, free of a bad pungent smell, free of sediment, and free of an effect on health of a worker, and does not need to be exchanged in the use procedure. The novel cadaver specimen preserving fluid is relatively ideal cadaver specimen preserving fluid in education and research at present, and is good substitute goods of formalin in long-term tissue preservation.

Description

technical field [0001] The invention belongs to the fields of medicine and teaching, in particular to a corpse specimen anticorrosion preservation solution. Background technique [0002] Formaldehyde has excellent tissue protein denaturation and fixation and anti-corrosion and anti-mildew effects, so it is widely used in the preparation and preservation of tissue specimens. When making tissue samples for anatomy teaching and scientific research, usually first use 10% formaldehyde solution to perfuse the tissue through large blood vessels to denature and fix the protein, and then soak the fixed tissue samples in 5% formaldehyde solution for preservation. In addition, there are 0.1-0.3% phenols, bromogeramine solution and so on. Chinese patent CN1081814A has reported a kind of preservative for soaking animal and plant specimens made by dissolving boric acid, sodium chloride and phenol in formaldehyde and alcohol solvent. [0003] However, formaldehyde is a teratogenic and ca...

Claims

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Application Information

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IPC IPC(8): A01N1/00
Inventor 李志远胡建光曹文宇邓思浩卢大华徐杨
Owner CENT SOUTH UNIV
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