In-vitro tissue preserving fluid and preparation method thereof
A technology for preserving fluid and tissue, applied in the field of medical preservation fluid and its preparation, can solve problems such as toxicity and the danger of human disease treatment, and achieve the effects of stable properties, good preservation effect and low transportation requirements
Active Publication Date: 2011-04-06
UNION STEMCELL & GENE ENG
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Problems solved by technology
In addition, because penicillin is easy to cause allergic reactions, and streptomycin is potentially toxic to the human body, and there is potential danger to the future
Method used
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preparation example Construction
[0039] The method for preparing a preservation solution for preserving isolated tissues as described above comprises the following steps:
[0040] (1) Prepare an aqueous deionized water solution of an osmotic pressure regulator, stir overnight until completely dissolved, and filter out impurities with a 50um filter membrane;
[0041] (2) Add the remaining solute into the above aqueous solution and stir until completely dissolved;
[0042] (3) Filter through 50um, 0.4nm, 0.2um, 0.1um filter membranes successively, and store the filtrate at room temperature for later use.
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The invention discloses in-vitro tissue preserving fluid and a preparation method thereof. Each 1,000 milliliters of preserving fluid comprises the following components by weight: 5 to 200 grams of osmotic pressure regulator, 0.2 to 40 grams of a pH value buffer pair, 3 to 15 grams of potassium chloride, 1 to 5 grams of sodium chloride, 10 to 300 milligrams of antibiotic, 5 to 100 grams of glucose and the balance of deionized water. In the invention, a non-CO2 dependent buffer pair is used to reduce the dependence on CO2 in transport and an air exposure process and reduce the fluctuations of the pH value; a hypertonic and high-potassium environment is used to reduce the swelling of cells and tissues and improve the activities of the cells and tissues; and the antibiotic used is free from causing hypersensitivity reaction. The preparation method is simple and in-vitro tissue preserving fluid has a remarkable in-vitro tissue preserving effect. The in-vitro tissue preserving fluid is low in cost, has low transport requirements, and can be stored at room temperature and conveniently.
Description
technical field [0001] The invention relates to a medical preservation solution and a preparation method thereof, in particular to a preservation solution for isolated human or animal tissues and a preparation method thereof. Background technique [0002] Organ transplantation is a major advance in human medical science in the 20th century. Since its clinical application in the 1960s, transplantation medicine has developed by leaps and bounds. However, any clinical organ transplantation must first have a high-quality donor, which is the prerequisite and fundamental guarantee for the success of organ transplantation. Therefore, organ preservation is of great significance here, and it is the cornerstone of organ transplantation. Over the past ten years, the number of organ transplants in China has leapt to the first place in Asia. However, in addition to kidney preservation, the preservation of organs such as liver, heart and pancreas still relies on imported preservation solu...
Claims
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Inventor 韩俊领孟恒星石釧
Owner UNION STEMCELL & GENE ENG
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