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Antibody-PD-L1-loaded bionic targeting TiO2 nano-particles, preparation method therefor and use of antibody-PD-L1-loaded bionic targeting TiO2 nano-particles

A PD-L1, nanoparticle technology, applied in the direction of antibodies, antibody medical components, anti-tumor drugs, etc., can solve the problems of insufficient targeting and efficacy, and achieve strong targeting aggregation ability, high antibody carrying rate, good The effect of the application foreground

Active Publication Date: 2020-12-25
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the prior art modified TiO 2 The application of nanometer in SDT has the problem of insufficient targeting and curative effect

Method used

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  • Antibody-PD-L1-loaded bionic targeting TiO2 nano-particles, preparation method therefor and use of antibody-PD-L1-loaded bionic targeting TiO2 nano-particles
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  • Antibody-PD-L1-loaded bionic targeting TiO2 nano-particles, preparation method therefor and use of antibody-PD-L1-loaded bionic targeting TiO2 nano-particles

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Embodiment 1

[0134] Example 1. The PD-L1-loaded antibody biomimetic targeting TiO of the present invention 2 Preparation of nanoparticles

[0135] 1. Mouse malignant melanoma B16F10 cell membrane extraction

[0136] (1) Collect B16F10 cells in 4 T175 cell culture flasks (about 1×10 8 cells);

[0137] (2) Centrifuge at 1000 g for 5 min, wash twice with PBS; resuspend cells with 1 mL of buffer. The pH of the buffer is 7.2, and 10 mM Tris(hydroxymethyl)aminomethane (Tris) is used as the solvent, and MgCl is added. 2 (100mM) 0.1mL and 100× EDTA-free protease inhibitor (10μL);

[0138] (3) Use an ultrasonic cell disruptor (power 300W, frequency 21KHz, 50% duty cycle) to disrupt cells for 30min under ice bath; collect supernatant after low temperature centrifugation (4000g) at 4°C for 10min;

[0139] (4) The supernatant was centrifuged at 4°C (16000g) for 30min at low temperature, and the supernatant was discarded and washed twice with PBS;

[0140] (5) ultrasonically resuspended with 1 mL...

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Abstract

The invention provides antibody-PD-L1-loaded bionic targeting TiO2 nano-particles, a preparation method therefor and use of the antibody-PD-L1-loaded bionic targeting TiO2 nano-particles and belongs to the field of biomedical materials. The TiO2 nano-particles are bionic TiO2 nano-particles loading a cell programed death acceptor-ligand 1 antibody; the bionic TiO2 nano-particles are prepared fromTiO2 nano-particles, DSPE-PEG2000-NH2 and cell membranes; and a mass ratio of the TiO2 nano-particles to the DSPE-PEG2000-NH2 to the cell membranes is (1 to 5): (1 to 5): (1 to 5). The nano-particlesare relatively small in particle size, meanwhile, are relatively high in antibody loading rate and are free of obvious cytotoxicity. Simultaneously, the nano-particles can be better conjugated to malignant melanoma cells in a homologous targeting and specific targeting manner and then cause apoptosis. In addition, the nano-particles have biological safety, have relatively high targeted aggregationcapability after being intratumorally injected and can better inhibit growth of malignant melanoma under SDT conditions. The nano-particles can be applied to treatment on tumors, particularlythe malignant melanoma and has a good application prospect.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a biomimetic targeting TiO carrying PD-L1 antibody 2 Nanoparticles and preparation methods and uses thereof. Background technique [0002] Malignant tumor is one of the most serious diseases that threaten human health and life in the world. At present, early-stage malignant tumors can be treated by traditional treatment methods such as surgery, chemotherapy, radiotherapy, etc., but these methods have little effect on patients with advanced tumors, and huge toxic and side effects such as liver and kidney damage may accelerate the progression of the patient's condition. deterioration and death. Therefore, there is an urgent need for some new therapeutic methods for adjuvant tumor therapy. With the continuous development of medical technology, some emerging therapies such as biological therapy, immunotherapy, photodynamic therapy and sonodynamic therapy are more and more widel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/02A61K39/395A61K41/00A61P35/00
CPCA61K9/143A61K39/3955A61K41/0033A61P35/00A61K2300/00
Inventor 邱逦马朗韦馨程冲向茜王丽芸朱笔挥李玲
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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