114results about How to "No apparent cytotoxicity" patented technology

The invention provides a nitrogen, phosphorus and sulphur doping or co-doping carbon dot and a batch controllable preparing method and application thereof. The method comprises the steps that a carbon source, a nitrogen source, a phosphorus source and a sulphur source are evenly mixed, and a mixture is obtained, wherein the molar ratio of C to N to P to S in the mixture is 1 to 0-0.8 to 0-0.4 to 0-0.4, and the contents of N, P and S are prevented from being zero at the same time; in the air, the mixture is heated to be fused, the reaction is carried out for 3 min to 60 min, natural cooling is carried out till the indoor temperature is reached, a reaction product is separated by a silicagel column, raw materials which do not react are removed, and the nitrogen, phosphorus and sulphur doping or co-doping carbon dot is obtained. According to the method, the technology is simple, the compound time is short, batch producing can be achieved, the doping amount can be adjusted and controlled accurately, the fluorescence color of the prepared carbon dot ranges from blue to green, the application can be achieved on bioluminescence marking and cell imaging aspects, and the good economic benefit and the application prospect are achieved.

The invention relates to a high-toughness corrosion-resistant magnesium alloy implanted material capable of being degraded in an organism, belonging to the technical field of biological materials. The material of the invention comprises the following components in percentage by weight: 1-4% of Nd, 0.1-1.0% of Zn, 0.1-1.0% of Ag, 0.3-0.8% of Zr and balance of Mg. The invention strengthens magnesium alloy through alloy elements, refines grains, improves plasticity, and further strengthens the magnesium alloy through extrusion deformation and heat treatment processes. The corrosion rate of the magnesium alloy provided by the invention in the simulated body fluid is 0.22-0.28mm / year, meeting the requirement of the implanted material for the corrosion rate; and the material of the invention does not have obvious cytotoxicity and has good blood compatibility, thereby meeting the requirements of the implanted material for biological compatibility. The high-plasticity medium-strength magnesium alloy provided by the invention can be used for support intravascular stent materials, and the high-strength medium-plasticity magnesium alloy provided by the invention can be used for implanted materials in orthopaedics.

The invention relates to a tumor-targeted T1-T2 double nuclear magnetic resonance imaging contrast agent and a preparation method and an application thereof. The contrast agent comprises hyaluronic acid-coated ferroferric oxide composite magnetic nanoparticles, wherein the molecular formula of hyaluronic acid is as shown in the specification; n is an integer of 17-290. The preparation method of the contrast agent comprises the following steps: (1) dissolving hyaluronic acid into deionized water, introducing a gas, and heating to obtain a reaction system A; (2) dissolving ferric salt and ferrous salt into a strong acid to obtain a solution B; (3) injecting the solution B into the reaction system A, adjusting the pH to be alkaline, and then refluxing at a high temperature to obtain a reaction system C; and (4) cooling a reaction system C to a room temperature, and dialyzing to obtain the contrast agent. The invention further provides an application of the contrast agent in T1 and T2 weighted imaging in in-vivo and in-vitro nuclear magnetic resonance. The contrast agent provided by the invention has superparamagnetism and outstanding T1 and T2 relaxation enhancement effects, and is suitable for being used as a T1-T2 double nuclear magnetic resonance imaging contrast agent.

The invention provides a nanofiber membrane and a preparation method and application thereof. The nanofiber membrane is formed by fibers of a core-shell structure and is characterized in that the core is formed by mixed fibers mixed by polylactic acid-hydroxyacetic acid and polycaprolactone, the shell is formed by fibers formed by gelatin crosslinking with genipin, and the core is loaded with chemical therapy medicine. The nanofiber membrane prepared by the coaxial electrospinning technology has an in-situ anti-cancer function and is implantable, good in biocompatibility, free of cytotoxicity to normal cells and good in curative effect on tumor. The preparation method is simple, mild in condition, low in cost and easy to popularize and apply.

The objective of the invention is to provide a novel beta type titanium alloy material with antibacterial and bone-healing-promoting functions and a preparation method thereof. According to the invention, proper amounts of copper, silver and rare earth elements are added into a Ti-Mo alloy. The novel beta type titanium alloy material comprises the following chemical components, by mass, 15 to 25%of Mo, 1 to 15% of Cu, 0.2 to 1% of Ag and 0.02 to 0.1% of La, with the balance being Ti. The novel beta type titanium alloy material is subjected to special heat treatment, so elemental copper is enriched on the surface of the titanium alloy and elemental silver is dissolved in a matrix in a solid solution manner; and thus, the novel beta type titanium alloy material is endowed with a biomedicalfunction and can be extensively applied to medical clinical fields such as orthopedics departments and stomatology departments using a variety of titanium alloy medical apparatuses and instruments. The novel beta type titanium alloy material overcomes the problems that conventional medical titanium alloys used in clinical practice has potential toxicity; the elastic moduli of the medical titaniumalloys are not matched with the modulus of the human body; the medical titanium alloys incur bacterial infection or are poor in osteogenesis states after implantation; etc.

The invention discloses an iron oxide / nano kaolin-containing composite hemostatic and a preparation method thereof. The composite hemostatic consists of nano kaolin and iron oxide, wherein the nano kaolin is adopted as a carrier; and the iron oxide is loaded on the surface of the nano kaolin. The composite hemostatic is prepared through a precipitation method, and has the advantages of being good in hemostatic effect, rapid in wound heal speed, free of conspicuous cytotoxicity, free of hemolysis, high in biocompatibility and the like.

The invention discloses a fullerene micro-nano material with effects of prevention and / or treatment on myelosuppression and a use thereof. The novel use of the fullerene micro-nano material comprises use of the fullerene and / or metallofullerene micro-nano material in a drug or a drug carrier with at least one of properties such as 1, prevention and / or treatment on myelosuppression, 2, prevention and / or treatment on at least one of myelosuppression-caused leucopenia, platelet decreasing, hemoglobin decreasing and monocyte decreasing, and 3, protection of liver tissue, spleen tissue and kidney tissue. The myelosuppression is caused through tumor radiation therapy or rays producing myelosuppression side effects. The fullerene and / or metallofullerene micro-nano material can prevent and treat myelosuppression and prevent liver, spleen and kidney damage.

The embodiment of the invention provides high-strength-and-toughness and corrosion-resistance magnesium alloy and a preparation method thereof. The alloy solves the problems in the prior art that thedeformation capability of the magnesium alloy is poorer and the plasticity processing is difficult when the magnesium alloy is used for preparing stents, the stents can be uniformly degraded in the body while the ideal supporting effect of alloy is achieved, better corrosion resistant performance is achieved, the corrosion-resistance rate is significantly reduced, and the corrosion is more uniform.

The invention relates to the technical field of tissue engineering, in particular to a degradable anastomosis pin in a living body and a production process for the degradable anastomosis pin. The anastomosis pin consists of the following components in percentage by mass: 2-6% of Zn, 2-5% of Mn, 0.1-1% of Sn, 1.5-2% of Ag, 0.5-2% of HA powder and the balance Mg. The degradable anastomosis pin in the living body can be naturally degraded in the living body, i.e., is degraded in the body within certain time after medical effect is achieved, so that economical burden and body burden, caused by second operation, on a patient are avoided; Al and rare-earth metal are avoided in aspect of component design, and biological safety of materials are guaranteed in aspect of material selection; the degradable anastomosis pin has high corrosion resistance, good plastic deformation capacity and good biocompatibility, and meets requirements, on a corrosion rate, of internal implant materials; and moreover, the degradable anastomosis pin is free of obvious cell toxicity and is good in blood compatibility, and can meet the requirements, on biocompatibility, of the internal implant materials.

The invention discloses a preparing method for anti-microbial hemostatic chitosan. The preparing method for the anti-microbial hemostatic chitosan comprises the steps that a cationic reagent solution is dropwise added in an epoxy reagent solution, the reaction temperature is controlled at 25-90 DEG C, reaction is conducted for 2-24h, pressure-reduction and distillation are conducted after the reaction is finished, and a quaternary ammonium reagent is obtained; the quaternary ammonium reagent is dissolved in a solvent, and a quaternary ammonium reagent solution is prepared; chitosan is added in an alkaline solution, then the quaternary ammonium reagent solution prepared in the step S1 is added and stirred for reaction, and the anti-microbial chitosan is obtained after washing and drying of raw products. The invention further discloses the application of the anti-microbialhemostatic chitosan. A chitosan anti-microbial hemostatic sponge is prepared, according to the standard of GB / T20944.3-2008, the detected bacteriostasis rate of staphylococcus aureus is greater than or equal to 98%, the bacteriostasis rate of escherichia coli is greater than or equal to 90%, and it is indicated that the prepared chitosan anti-microbial hemostatic sponge has the anti-microbial effect; the hemostatic effect of the prepared anti-microbial hemostatichemostatic sponge is excellent, and hemostatic time is less than or equal to 60 seconds; and the anti-microbial hemostatic sponge prepared by utilizing the chitosan has no obvious cytotoxicity, and biodegradation can be achieved.

The invention relates to an imaging agent. The imaging agent is characterized by comprising IR820-CSQ-Fe nanoparticles, wherein the IR820-CSQ-Fe nanoparticles comprises Fe3O4 coated with an A-X-B structure, wherein A is chitosan quaternary ammonium salt CSQadopting a structure shown as a formula (I), and n is an integer ranging from 17 to 290; B is new indocyanine green IR820 adopting a structure shown as a formula (II); X is a compound adopting a structure shown as a formula (III), and R is an alkylene group. The imaging agent has the advantages of superparamagnetism, outstanding T1 and T2 relaxation enhancement effect, photoacoustic imaging capacity, obvious fluorescence spectra, small and uniform particle size, good penetrability, avoidance of obvious cytotoxicity, good biocompatibility and better long circulation effect; a preparation method is simple, mild in condition and lower in cost; the imaging agent can be freeze-dried and stored for a long time in a solid form.

The invention relates to the technical field of gene engineering, in particular to novel broad-spectrum chimeric lysin BGS-PlySb and an encoding gene and application thereof. The novel broad-spectrum chimeric lysin BGS-PlySb has an amino acid sequence shown as in SEQ ID NO. 1; the encoding gene of the novel broad-spectrum chimeric lysin BGS-PlySb has a nucleotide sequence shown as in SEQ ID NO. 2. A novel chimeric lysin is constructed by means of gene splicing and is suitable for killing various species of Streptococcus and Staphylococcus, particularly various species of Enterococcus, and Staphylococcus aureus, as well as Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus suis, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus mutans, Streptococcus iniae and other species; the novel broad-spectrum chimeric lysin BGS-PlySb has good stability and is insensitive to high-concentration NaCl, recombinant protein GBS-PlySb is well expressible in Escherichia coli BL21 (DE3), and a high dose of GBS-PlySb is free of significant cytotoxicity. Therefore, the novel broad-spectrum chimeric lysin BGS-PlySb is applicable independently to or as an additive to the control of various species of Streptococcus and the treatment of infections caused by these species, and has a promising application prospect.

The invention provides a wound dressing based on hydrogel-core-shell microspheres and a preparation method thereof, and belongs to the technical field of wound dressings. The preparation method includes the steps that a coaxial electrostatic spraying method with an RCSPs solution serving as the inner axis and a sodium alginate solution serving as the outer axis is adopted, and formed microdropletsare collected into a calcium chloride solution for crosslinking to obtain sodium alginate / RCSPs core-shell microspheres; gelatin and a polyvinyl alcohol solution are mixed to obtain a homogeneous gelprecursor, the sodium alginate / RCSPs core-shell microspheres are added into the homogeneous gel precursor, the mixture is stirred to be uniform, and then freeze-drying is performed to obtain the wound dressing based on the hydrogel-core-shell microspheres. The invention further provides the wound dressing based on the hydrogel-core-shell microspheres obtained by the preparation method. The core-shell microspheres can achieve a high encapsulation rate on drugs, the combined application of the core-shell microspheres and hydrogel effectively achieves slow release of the drugs, and the wound dressing can act on wounds with a long-lasting effect and significantly promote wound healing.

The invention relates to a preparation method of FGF1 sericin protein gel with activity for promoting cell proliferation and a product thereof. The preparation method comprises the following steps that sericin protein containing human FGF1 protein is extracted by human FGF1 gene modifying silk, and a sericin protein solution containing human FGF1 protein is obtained; after renaturation, an FGF1 sericin protein aqueous solution is obtained after dialysis with water, and FGF1 sericin protein hydrogel is obtained by centrifugation; and the FGF1 sericin protein hydrogel is placed at 4 DEG C, an FGF1 sericin protein gel material is formed by cross-linking of a chemical cross-linking agent or inducement of an inducer. The prepared gel material has the activity of promoting cell proliferation andhas broad application potential in tissue engineering.

The invention provides a polyelectrolyte composition hydrogel hemostatic based on polysaccharide / polypeptide. The polyelectrolyte composition hydrogel hemostatic is prepared from a polysaccharide solution and a polypeptide solution through mixing and physical crosslinking, wherein the polysaccharide solution comprises a xanthan gum; and the polypeptide solution comprises polylysine. After the polysaccharide solution and the polylysine solution of different ratios are mutually mixed, physical crosslinking is implemented under electrostatic interactions of molecules and interactions of hydrogenbonds of the molecules, and then hydrogel is rapidly generated. Because of advantages of being rapid in gelatinization, good in biocompatibility, and the like, the hydrogel can be used as the hydrogelhemostatic and is applied to trauma hemostasis and surgical hemostasis.

The invention relates to healthcare liquor, and specifically relates to rosemary healthcare medicinal liquor. The efficacies of existing qi invigorating, blood nourishing, yin-yang regulating, and viscera tonifying healthcare medicinal liquor are poor, and the healthcare medicinal liquor is not suitable for large-quantity and long-term consumption. With the medicinal liquor provided by the invention, the problems are solved. A manufacturing method of the medicinal liquor comprises steps that: (1) fresh rosemary leaves are washed and are dried by filtering; the leaves are places in a microwaveoven; the leaves are first baked under a high temperature, and then baked under a middle temperature; (2) Mongolian milkvetch root and ligusticum are washed, sliced, and dried; and medlar is washed and dried; (3) the traditional Chinese medicines in the steps (1) and (2) are placed into an alcohol extraction pot, and are soaked by using distillate spirit with an alcoholicity of 50; slag is removed, and a leaching solution is obtained; (4) the leaching solution is placed in a storing pot; distillate spirit with an alcoholicity of 50 is added into the pot; the mixture is stirred and stood, suchthat a mother liquor is obtained; (5) yellow rice wine with an alcoholicity of 24, rock sugar or stevioside, and purified water are added to the mother liquor; the mixture is stirred and stood, such that the rosemary healthcare medicinal liquor is prepared. The rosemary healthcare medicinal liquor provided by the invention has functions of qi invigorating, blood nourishing, yin-yang regulating, viscera tonifying, and the like. The effects are good, and the alcohol content is relatively low, such that the rosemary healthcare medicinal liquor is suitable to be taken for a long time.

The invention relates to an electrospinning membrane with adsorption and filtration function and a preparation method and applications thereof The preparation method of the electrospinning membrane comprises following steps: (1) polylactide-glycolic acid and / or polycaprolactone are dissolved in hexafluoroisopropanol solvent and stirring is performed to obtain a solution A; (2) after the solution A is stirred at room temperature overnight, electrospinning is performed with the above solution A to obtain membrane; (3) freeze drying and vacuum drying are performed on the obtained membrane. The membrane material of the invention is characterized in that the material is a multi-layer and porous membrane material formed by fiber having the diameter range from 400 to 900nm. The preparation process is simple; the membrane can be used for effectively preventing infestation of haze; the membrane has high filter and protecting performance and can be widely used in the field of haze protection products.

The invention provides a reduced sensitive type polymer with the effect of arginine membrane penetration as well as a preparation method of the reduced sensitive type polymer. The polymer can self-assemble in an aqueous medium to form micelle of a shell-core structure; the polymer contains a poly-L-arginine structure, so that the speed that the polymer penetrates through a cell membrane to enter cancer cells is increased; meanwhile, the polymer contains a disulfide bond, so that the polymer can be degraded by relatively high reduction potential energy in cancer cells. The invention further provides application of the reduced sensitive type polymer with the effect of arginine membrane penetration as a drug carrier. After being gathered at tumor tissue through the enhanced permeability and retention effect, the carrier can quickly enter the cancer cells, so that bioavailability and the treatment effect of the drug can be improved.

The invention relates to the technical field of genetic engineering and particularly relates to a streptococcus broad-spectrum chimeric lyase GBS-V12b and a coding gene and application thereof. The streptococcus broad-spectrum chimeric lyase GBS-V12b has an amino acid sequence shown in SEQ ID No. 1. The coding gene of the streptococcus broad-spectrum chimeric lyase GBS-V12b has a nucleotide sequence shown in SEQ ID No. 2. The streptococcus broad-spectrum chimeric lyase GBS-V12b disclosed by the invention can be used for killing a variety of streptococci and staphylococci, particularly streptococcus pneumoniae, streptococcus pyogenes, streptococcus suis, streptococcus agalactiae, streptococcus dysgalactiae, streptococcus mutans, streptococcus iniae, and a variety of enterococci and staphylococcus aureus; the GBS-V12b is good in stability and is insensitive to high-concentration NaCl; the recombinant protein GBS-V12b can be excellently expressed in Escherichia coli BL21 (DE3); high-dosage GBS-V12b is free of obvious cytotoxicity. Therefore, the GBS-V12b can be used for controlling a variety of streptococci and treating infections caused by the streptococci independently or as an additive, thereby having a broad application prospect.

The invention relates to a controlled-release hydrogen sulfide donor. The donor is obtained by reacting diallyl trisulfide with an ordered mesoporous silicon dioxide nanoparticle materials. The invention also provides a preparation method and application of the hydrogen sulfide donor. The controlled-release hydrogen sulfide donor is capable of slowly releasing medium-concentration hydrogen sulfide, can be regulated by use of glutathione and has no obvious cytotoxicity; as a result, the controlled-release hydrogen sulfide donor is an ideal hydrogen sulfide donor for in-vivo and in-vitro experiments and clinical application.

The embodiment of the invention provides a corrosion-resistant high strength and toughness magnesium alloy tubular product and a preparation process. The alloy tubular product is prepared from the following elements in percent by mass: 1.0-6.0% of Nd, 0.1-5.0% of Zn, 0.1-5.0% of Ca, 0.1-4.0% of Zr and the balance Mg. The preparation process comprises the following steps: successively preparing a magnesium alloy cast ingot, a magnesium alloy blank, a magnesium round bar and a magnesium hollow round ingot through a series of steps: vacuum semicontinuous casting, solid solution homogenizing, barextrusion processing, hollow round ingot processing and the like; and finally, extruding the hollow round ingot to obtain the corrosion-resistant high strength and toughness magnesium alloy tubular product. The corrosion-resistant high strength and toughness magnesium alloy tubular product solves the problem of hard plastic processing for preparing a bracket as the magnesium alloy tubular productis relatively poor in deformability at room temperature in the prior art, can be degraded uniformly in vivo while achieving an ideal supporting effect of the alloy, and meanwhile, has better corrosionresistance and is relatively uniform to corrode, wherein the corrosion rate is 0.18-0.42 mm / year.

The invention discloses applications of a fullerene structure in preparation of drugs used for treating leukemia. The fullerene structure is taken as an active ingredient, and comprises at least one ingredient selected from an oil soluble fullerene, an oil soluble metal embedded fullerene, a composition of the oil soluble fullerene and the oil soluble metal embedded fullerene, a water soluble fullerene, a water soluble metal embedded fullerene, a composition of the water soluble fullerene and the water soluble metal embedded fullerene, and a pharmaceutical ester or a pharmaceutical salt of theabove six ingredients. The active ingredient is capable of accumulating in bone marrow at a high efficiency; effects are achieved quickly; excellent biocompatibility is achieved; the fullerene structure can be used for preventing and treating leukemia before leukemia model maturation; after leukemia onset, the active ingredient possesses bone marrow part targeted enrichment characteristics, and can be used for rapid prevention, treatment, and repairing of bone marrow haematopoietic function damages by leukemia and other organic damages.

The invention relates to the technical field of tissue engineering, in particular to a degradable anastomosis pin in a living body and a production process for the degradable anastomosis pin. The anastomosis pin consists of the following components in percentage by mass: 2-6% of Zn, 2-5% of Mn, 0.1-1% of Sn, 1.5-2% of Ag, 0.5-2% of HA powder and the balance Mg. The degradable anastomosis pin in the living body can be naturally degraded in the living body, i.e., is degraded in the body within certain time after medical effect is achieved, so that economical burden and body burden, caused by second operation, on a patient are avoided; Al and rare-earth metal are avoided in aspect of component design, and biological safety of materials are guaranteed in aspect of material selection; the degradable anastomosis pin has high corrosion resistance, good plastic deformation capacity and good biocompatibility, and meets requirements, on a corrosion rate, of internal implant materials; and moreover, the degradable anastomosis pin is free of obvious cell toxicity and is good in blood compatibility, and can meet the requirements, on biocompatibility, of the internal implant materials.

The invention provides magnetic nano pharmaceutical complex and a preparation method application thereof. The magnetic nano pharmaceutical complex comprises ferroferric oxide nanoparticle doped polypyrrole nano-carrier, pharmaceutical DAPT carried on the surface of the polypyrrole nano-carrier, and hyaluronic acid adsorbed to the surface of the polypyrrole nano-carrier that carries the pharmaceutical DAPT. The magnetic nano pharmaceutical complex has high ability to carry the pharmaceutical DAPT, good stability and small particle size, has good penetrating capacity and uniform particle size, has significant magneto-thermal slow control, has long-cycle effect in vivo, has no evident cytotoxicity, has low long-term toxicity and good biocompatibility, has significant killing effect on tumor stem cells, and has better killing effect particularly in connection with magneto-thermal therapy; the preparation method of the complex is simple, has mild conditions and low cost and is easy to popularize and apply.

The invention discloses polysubstituted thieno[2,3-b]pyridine derivatives, and a preparation method and an application thereof. The structural formula of the derivatives are represented by formula I shown in the description. A compound for inhibiting a urea transporter is screened by using an erythrocyte model. Experimental results show that the compounds (represented by formula I-1) can inhibit the permeation of a urea transporter UT-B mediated erythrocyte membrane to urea, and the effect of the compounds has a dose-dependent relationship; the compounds represented by the formula I-1 have nocytotoxic effect on MDCK cells within an effective dose range, so the effect of the compounds represented by the formula I-1 on inhibiting cell permeation urea is irrelevant to the cytotoxicity of thecompounds; the inhibition effect of the compounds represented by the formula I-1 on the urea transporter UT-B is gradually enhanced; the inhibiting effect of the compounds represented by the formulaI-1 on the UT-B is reversible; and in-vivo test results show that the compounds represented by the formula I-1 can significantly increase the urination volume of rats, reduce the concentration of ureain rat urine and reduce the osmotic pressure, so that the compounds represented by the formula I-1 generate a urea selective diuresis effect in vivo.

The invention belongs to the technical field of oral tumor resistance, and discloses an oral tumor resistant pH sensitive intelligent material and an application thereof; the oral tumor resistant pH sensitive intelligent material is a tertiary ammonium monomer, and the tertiary ammonium monomer is methylacrylic acid methyl dodecyl amino ethyl ester, namely DMAEM. The invention provides the application of the oral tumor resistant pH sensitive intelligent material in drugs for inhibiting growth of oral squamous cell carcinoma cells, inhibiting tumor growth caused by the oral squamous cell carcinoma cells and preventing or treating the oral squamous cell carcinoma. The oral tumor resistant pH sensitive intelligent material provided by the invention can inhibit proliferation of tumor oral squamous cell carcinoma cells and promote apoptosis of oral squamous cell carcinoma under a biosafety concentration; and in addition, the material has a pH sensitive property, and the proliferation inhibiting and apoptosis promoting effects of the material are enhanced in an acid environment, namely an environment with the pH being 6.7. Animal experiments prove that the material has anti-tumor performance.

The invention provides antibody-PD-L1-loaded bionic targeting TiO2 nano-particles, a preparation method therefor and use of the antibody-PD-L1-loaded bionic targeting TiO2 nano-particles and belongs to the field of biomedical materials. The TiO2 nano-particles are bionic TiO2 nano-particles loading a cell programed death acceptor-ligand 1 antibody; the bionic TiO2 nano-particles are prepared fromTiO2 nano-particles, DSPE-PEG2000-NH2 and cell membranes; and a mass ratio of the TiO2 nano-particles to the DSPE-PEG2000-NH2 to the cell membranes is (1 to 5): (1 to 5): (1 to 5). The nano-particlesare relatively small in particle size, meanwhile, are relatively high in antibody loading rate and are free of obvious cytotoxicity. Simultaneously, the nano-particles can be better conjugated to malignant melanoma cells in a homologous targeting and specific targeting manner and then cause apoptosis. In addition, the nano-particles have biological safety, have relatively high targeted aggregationcapability after being intratumorally injected and can better inhibit growth of malignant melanoma under SDT conditions. The nano-particles can be applied to treatment on tumors, particularlythe malignant melanoma and has a good application prospect.

The invention discloses a composite membrane carrying bioactive factors PLA / PLGA / CS and a preparation method thereof. The mode of combining electrostatic spinning and electrostatic spraying is utilized, a PLA membrane is taken as a sealing layer, the diffusion of drugs can be prevented by utilizing the compactness of the PLA membrane, and mechanical support is provided; PLGA microspheres are takenas a sandwich layer to carry the drugs; and the large porosity and high biocompatibility of a CS membrane are utilized as a controlled-release layer to directly contact with damaged areas, and the purposes of neural restoration can be achieved by planning seed cells can be achieved, so that the PLA / PLGA / CS composite membrane with membrane / ball / membrane structures can be prepared. The composite membrane is stable in structure and has a certain mechanical strength, the tensile strength is 2.83+ / -0.31 Mpa, and the elongation at break is 53.25+ / -0.18%; the composite membrane can well carry the drugs and has good drug sustained-release effects, and the releasing of the drugs can achieve more than two months, so that the time required by the repairing of spinal cord injury can be met; and the composite membrane has no cytotoxicity and can successfully induce PC-12 cells to differentiate into neurons within 5 days, so that the composite membrane is good for neural restoration.

The invention discloses a hericium erinaceus polysaccharide chelated zinc microcapsule and a preparation method thereof. The method includes utilizing a hericium erinaceus polysaccharide as a raw material, chelating the hericium erinaceus polysaccharide with zinc ions to form a polysaccharide zinc chelate, performing homogenization emulsification and spray drying with carrageenan: chitosan: beta-cyclodextrin as wall materials and performing microencapsulation treatment to obtain the hericium erinaceus polysaccharide chelated zinc microcapsule. The microcapsule overcomes shortcomings of poor antioxidant activity of the traditional hericium erinaceus polysaccharide and low zinc content in the hericium erinaceus polysaccharide chelated zinc (Zn-HEP), can not only improve the dispersibility and the emulsifying dispersibility, but also protect the easily oxidized components in the polysaccharide, enhance stability and antioxidant activity and prolong the shelf life. The prepared hericium erinaceus polysaccharide chelated zinc microcapsule has obvious anti-oxidation activity, and enhances immune function and anti-cancer effect. The preparation method is simple and convenient, capable ofachieving industrial continuous production, and has wide practical value.

The invention relates to the field of water pollution treatment, in particular to application of a microcystin degrading enzyme in degradation of nodularin. The microcystin degrading enzyme is immobilized to L-cysteine-modified graphene oxide material; the steps include (1) activating GO (graphene oxide) with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimidesodium salt, and modifying the activated GO with L-cysteine to obtain CysGO solution; (2) adding mlrA enzyme liquid into the CysGO solution, mixing well, carrying out shock reaction at 0 DEG C for 60min to obtain immobilized enzyme nano composite CysGO-mlrA solution, filtering the solution with a 0.22 mu m ultrafiltration membrane, washing the obtained solid with water, carrying out suction filtering, and drying to obtain the immobilized enzyme nano composite CysGO-mlrA. It is discovered by the inventor via experiments that the microcystin degrading enzyme can well degrade nodularin; CysGO-mlrA and NOD degraded products have no evident cytotoxicity to lymphocytes in zebrafish.