The invention provides sika deer coronet antibacterial peptides / proteins with antibacterial effect and applications thereof, including sika deer coronet micromolecular polypeptides, sika deer coronet collagen hydrolyzing peptides, and sika deer coronet micromolecular protein monomers. The peptides / proteins are subjected to inhibition ring tests so as to observe the inhibiting effect on Gram-negative bacteria, Gram-positive bacteria, and fungi; wherein the Gram-negative bacteria comprise enterobacter aerogenes, proteus mirabilis, pseudomonas aeruginosa, and escherichia coli. The Gram-positive bacteria comprise staphylococcus lentus, lactococcus garvieae, staphylococcus xylosus, bacillus subtilis, and staphylococcus intermedius. The fungi are candida albicans. Under the effect of various bacteriostatic agents, the enterobacter aerogenes, bacillus subtilis, escherichia coli, pseudomonas aeruginosa, staphylococcus intermedius, and staphylococcus xylosus are inhibited to a certain degree, and the candida albicans, lactococcus garvieae, proteus mirabilis, and staphylococcus lentus are not inhibited.