62 results about "Staphylococcus albus" patented technology

The application discloses a fabric yellowing reagent and a fabric yellowing method, and relates to the technical field of daily chemical products. 5 The fabric yellowing reagent comprises 1-3% of histidine solution and a bacterial suspension with a bacterial concentration of 1*10 5 CFU / mL-9*10 The bacteria are a mixture of one or more of Staphylococcus epidermidis and Micrococcus luteus; the solvent of the histidine solution is normal saline containing 0.15-1% of trypsin peptone; and the solvent of the bacterial suspension is normal saline containing 0.1-0.2% of trypsin soybean peptone liquid culture medium. The fabric yellowing reagent can be used for simulating fabric yellowing, and has good operability, reproducibility and data reliability.

The present invention relates to the field of biological fermentation application technology, and in particular, to use of a milk peptide fermentation product in regulating skin microecological balance. The milk peptide fermentation product selectively regulates the growth of Staphylococcus epidermidis and inhibits the growth of Staphylococcus aureus and Escherichia coli. It was discovered for the first time that the milk peptide fermentation product has a selective bacteriostatic effect on skin microorganisms. The experimental results show that the milk peptide fermentation product has an inhibitory effect on Escherichia coli and Staphylococcus aureus, but has no effect on the normal growth of Staphylococcus epidermidis. This characteristic is obviously different from common broad-spectrum chemical bacteriostatic agents, and the use of the milk peptide fermentation product is more conducive to maintaining the balance of skin microbiota.

The application discloses a composition capable of effectively inhibiting the growth of Staphylococcus aureus biofilm. The application firstly optimizes the nitrogen source in the culture medium of Staphylococcus epidermidis CCSM0287 strain, fully explores the growth difference of Staphylococcus epidermidis under different culture media and culture conditions and the inhibition effect of the fermentation supernatant on Staphylococcus aureus biofilm, then compounds the fermentation supernatant of Staphylococcus epidermidis CCSM0287 obtained by using the optimized culture medium (the nitrogen source adopts tryptone + soybean peptone + fresh beef bone collagen peptide powder) with the fermentation supernatant of Lactobacillus paracasei S20, can obviously inhibit the growth of Staphylococcus aureus biofilm and destroy the surface structure of the Staphylococcus aureus biofilm, and can be applied in bacteriostatic products.

The invention belongs to the technical field of probiotics, and particularly relates to plant lactobacillus with bacteriostatic activity and application of the plant lactobacillus. Wherein the plant lactobacillus with bacteriostatic activity is separated from the intestinal tract of a healthy human body, and the LV1000 fermentation filtrate freeze-dried powder is prepared through fermentation, centrifugation, filtration, ultrafiltration concentration and vacuum freeze-drying by taking inactivated staphylococcus epidermidis bacterial suspension as an inducer through a specific induction fermentation culture medium. Meanwhile, the freeze-dried powder is compounded with an embedding compound, galactooligosaccharide, beta-cyclodextrine, erythritol and the like, so that the fermented beverage capable of improving the intestinal microecology is prepared. The freeze-dried powder of the LV1000 fermentation filtrate can specifically inhibit enteropathogenic bacteria, the tea polyphenol in the embedded compound can further synergistically enhance the bacteriostatic action and exert the antioxidant effect, and the fermented beverage which is high in stability, fine and smooth in taste, free of refrigeration and capable of being directly drunk and improving the intestinal microecology is prepared.

To provide a composition effective for treating microbial skin infections, atopic dermatitis and the like.SOLUTION: Methods and compositions comprising hogocidin peptides (SH-lantibiotics), derivatives and variants are provided. Also provided are methods and compositions, including probiotic compositions, that utilize strains of Staphylococcus hominis and Staphylococcus epidermidis that produce hogocidin, hogocidin-like peptides, or other inhibitors of skin pathogens. Methods of treating microbial skin infections and atopic dermatitis are also provided.SELECTED DRAWING: Figure 1A-B

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 9I and 16F replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-11. Compared to the template peptide PL-0, the stapling peptide PL-11 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

Described is a method for treating or preventing an infection, such as an infection associated with Staphylococcus epidermidis such as a Health-Care Associated infection, a wound, a sore, an ulcer, a burn. The method includes applying a treatment preparation to a treatment site, the treatment preparation has a first component having a single amino acid, said single amino acid being leucine, and a second component having an active chlorine compound.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 6V and 13V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-10. Compared to the template peptide PL-0, the stapling peptide PL-10 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

The invention discloses an application of a bupleurum chinense extract in preparation of an antibacterial product. According to the invention, it is found for the first time that various extracts (including aqueous extracts, ethanol extracts and extraction parts of different polar solvents) of bupleurum chinense have relatively good bacteriostatic activity on streptococcus pneumoniae, methicillin-resistant staphylococcus epidermidis, micrococcus luteus and enterococcus faecium. The antibacterial spectrums and effects of different extracts are different, and the research lays a solid foundation for developing novel anti-drug-resistant bacteria drugs and natural-source antibacterial products.

The invention discloses a plant composition for regulating scalp micro-ecology and application thereof, and relates to the technical field of cosmetics. The invention provides a plant composition which is prepared from magnolia bark and uncaria rhynchophylla, and the feeding ratio of the magnolia bark to the uncaria rhynchophylla is 7: 3-3: 7. The plant composition has the effect of synergistically promoting expression of scalp keratinocyte human beta-defensin, has an inhibiting effect on malassezia and does not have an inhibiting effect on staphylococcus epidermidis under the concentration of 0.5%-2%, has the effect of adjusting scalp flora balance, and can relieve the degree of scalp itching and reduce the number of scurf.

The invention discloses torreya grandis seed oil and application thereof, and belongs to the field of plant extracts. The torreya grandis seed oil is prepared by adopting a supercritical CO2 extraction method, the expression level of a macrophage RAW264.7 inflammatory factor IL-6 caused by LPS can be remarkably reduced, and the torreya grandis seed oil has a good anti-inflammatory effect. The traditional Chinese medicine composition disclosed by the invention can effectively inhibit the growth of propionibacterium acnes, also can effectively regulate the skin flora balance of people with acnes, has no obvious influence on the growth of staphylococcus epidermidis while remarkably inhibiting the growth of propionibacterium acnes, is an excellent acne-removing cosmetic raw material, and has a wide application prospect in the development of nursing products for people with acnes.

The invention discloses staphylococcus epidermidis CCSM009 for repairing a skin barrier and a metagen of the staphylococcus epidermidis CCSM009, and belongs to the field of microbial technologies and medicines. The staphylococcus epidermidis CCSM009 and the metagen thereof provided by the invention have a good barrier function repairing effect and application prospect in the aspect of external use, and are specifically shown as follows: the activity of damaged HaCaT cells is improved in vitro, and the expression of gene levels and protein levels in the aspects of functional proteins and structural proteins after damage is improved; meanwhile, sphingomyelinase 1 and tissue kininolase 7 (KLK7) are improved, and it is clear that the bacterium performs up-regulation of skin barrier related proteins and enzymes through an AHR-OVOL1 pathway. Therefore, the metagen of the staphylococcus epidermidis CCSM009 has a huge application prospect in preparation of cosmetics or medicines for repairing the skin barrier.

The invention discloses a staphylococcus epidermidis fermentation product, a preparation method thereof and application of the staphylococcus epidermidis fermentation product in preparation of anti-inflammatory drugs. The method comprises the following steps: inoculating staphylococcus epidermidis into a TSB culture medium for culture, and after culture is finished, sequentially centrifuging and filtering the obtained fermentation liquor to finally obtain fermentation supernate, namely a staphylococcus epidermidis fermentation product. It is found that the optimized staphylococcus epidermidis fermentation product has an anti-inflammatory effect, generation of proinflammatory factors such as NO, IL-6 and TNF-alpha can be remarkably reduced, and therefore the staphylococcus epidermidis fermentation product can be applied to the industry of research and development of soothing cosmetics, and the effect of the product is enhanced.

The invention relates to the field of daily chemicals, and discloses a plant-derived sterilization deodorant and application thereof in daily chemical products. The sterilizing deodorant comprises at least two, preferably three, of pepper leaf extract, celery leaf extract and syzygium jambos leaf extract, and is matched with decyl glucoside, citric acid, a chelating agent and an optional deep eutectic solvent to form a stable water-alcohol system. Through specific proportion compounding and process condition control, efficient bacteriostasis on staphylococcus aureus, staphylococcus epidermidis, candida albicans and trichophyton rubrum is achieved, meanwhile, the concentration of odor substances is remarkably reduced, and the deodorant has the functions of source bacteriostasis and direct deodorization. The physical stability and the antibacterial activity retention rate of the system are further improved by adopting a deep eutectic solvent composed of glycerol and choline chloride, so that the deep eutectic solvent is suitable for preparing daily chemical products such as shoe and sock spray, body spray and fabric freshener.

The application discloses a natural product 3-methylcarbazole derivative with a general formula B and a preparation method and application thereof in the technical field of organic chemistry. S. aureus S. epidermidis The derivative has good antibacterial activity, can significantly inhibit Staphylococcus aureus, Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus (MRSA), and can be used for preparing a drug for treating related bacterial infections.​

The invention relates to the technical field of cosmetics, and provides skin repair essence taking a staphylococcus epidermidis fermentation product as a core, the skin repair essence is prepared from a core repair component, a moisturizing and soothing component and a mild auxiliary component in a synergistic compatibility manner by weight: the core repair component comprises 1.0-10.0% of staphylococcus epidermidis fermentation product filtrate and 0.5-2.0% of Ectoine; a moisturizing and soothing component: 0.1 to 0.5 percent of small molecular sodium hyaluronate, 0.2 to 1.0 percent of panthenol, 0.3 to 1.5 percent of beta-glucan and 5.0 to 15.0 percent of a moisturizing agent; and a mild auxiliary component: 0.3-0.8% of a mild preservative, and the balance of deionized water. The staphylococcus epidermidis fermentation product is taken as a core to be compounded with multiple repairing components to form a synergistic system, so that the skin micro-ecology can be regulated, harmful bacteria can be inhibited, damaged barriers can be repaired, deep moisturizing can be realized, and red discomforts can be relieved. The formula is mild and non-irritant, is adaptive to sensitive skin and scenes after medical art, is stable and controllable in process, fully covers multiple scenes, improves skin stability from the source, and is superior to traditional products in use experience and repair effect.

This invention belongs to the field of peptide drugs, specifically relating to an antibacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 13V and 20V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-13. Compared to the template peptide PL-0, the stapling peptide PL-13 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

The invention belongs to the field of polypeptide drugs, and particularly relates to an antibacterial polypeptide and application thereof. According to the invention, Rink amide MBHA amino resin is used as a solid phase carrier, modification and transformation are carried out according to an amino acid sequence of a template polypeptide raniseptinPL: Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2, and on the basis of retaining key amino acid residues, original amino acids are respectively replaced by S5 and R8 at amino acid positions i, i + 4 and i and i + 7, so that a target stapling peptide is obtained. Compared with a template polypeptide PL-0, the obtained stapled peptide has the advantage that the antibacterial activity on staphylococcus aureus, enterococcus faecium, enterococcus faecalis and staphylococcus epidermidis can be obviously improved.

The application belongs to the field of polypeptide drugs, and particularly relates to an antibacterial polypeptide and application thereof in pharmacy. The application takes Rink amide MBHA amino resin as a solid phase carrier, and modifies and transforms according to a template polypeptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 amino acid sequence, wherein amino acid residues 19G and 26N are replaced by R8 and S5, to obtain a target stapled peptide PL-16. The stapled peptide PL-16 can significantly improve the antibacterial activity on Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis and Staphylococcus epidermidis relative to the template polypeptide PL-0.

Blepharitis, an infection and inflammation of the edge of the eyelids, and meibomian gland dysfunction (MGD), blockage of tiny oil glands at the eye lid margin, are quite common, usually under diagnosed and underappreciated diseases. There are number of causes for blepharitis, including bacterial components such as Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Corynebacteria sp., seborrhea or microscopic mites (Demodex). More than 50% of patients seeking ophthalmologic consultations have symptoms and signs indicating one of these entities. Another large number of patients remain undiagnosed or in early stages of disease that could be easily treated thus preventing long-term pain and suffering. MGD is in fact the leading cause of dry eye disease, a potentially preventable malady.

The application discloses a fusion membrane of Staphylococcus epidermidis vesicles and curcumin-derived exosome-like vesicles and a preparation method and application thereof. The application fuses Staphylococcus epidermidis vesicles and curcumin-derived exosome-like vesicles to obtain HMVs, and verifies the antibacterial effect of the HMVs through in-vitro experiments, and then the HMVs are loaded in hyaluronic acid microneedles. The HMVs MNs nano system can be locally administered by pressing on acne lesions, and the in-vivo experiments on Balb / c mice prove that the system has relatively remarkable antibacterial and anti-inflammatory effects. Therefore, the HMVs MNs have relatively good application potential in treating acne.

The present invention relates to isolated live bacterial strains of the species Staphylococcus epidermidis selected from the group consisting of the bacteria deposited at the CNCM under deposit numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, as well as compositions comprising them. The present invention also relates to the use of such strains for preventing a decline in and / or strengthening the skin barrier function in an individual, for improving skin moisturization, for improving skin quality, in particular the quality of dry skin, for preventing and / or treating cosmetic signs associated with dry skin, in particular selected from tightness, itching, discomfort, a dull complexion, rough skin and pronounced microrelief of the skin, and / or for preventing and / or treating pruritus, in particular pruritus of skin affected by skin disorders, such as atopic dermatitis, and / or for the prevention and / or treatment of atopic dermatitis, as well as to a non-therapeutic cosmetic method for caring for keratinous materials, said non-therapeutic cosmetic method comprising at least one step of topically applying such strains or compositions to said keratinous materials.

A genetically modified strain of commensal bacteria Staphylococcus epidermidis is provided which produces a material with UV absorbing properties. The material with UV absorbing properties may be a mycosporine-like amino acid such as shinorine.

The invention discloses a preparation method and application of an antibacterial biofilm liquid dressing with a hemostatic effect and capable of quickly targeting bacteria. The antibacterial biological membrane liquid dressing is prepared from the following components in percentage by weight: 5 to 10 percent of TMG-Ag < + >-EVs coordination liquid, 60 to 70 percent of ramie fiber pulp with the solid content of 0.2 to 0.5 percent, 10 to 20 percent of ramie water extract and 0.15 to 2 percent of povidone K30, wherein the TMG-Ag < + >-EVs coordination liquid contains betaine with the final concentration of 50 to 70 mM, silver ions with the final concentration of 20 to 30 [mu] g / ml and a staphylococcus epidermidis bacterial membrane with the final concentration of 500 to 1000 ng / ml. The betaine, the silver ions and the ramie are combined, bacterial biofilms can be efficiently destroyed, bacterial colonization is inhibited, the toxicity of the silver ions is reduced, and the composition is suitable for prevention and treatment of infection of various wound types such as skin and mucous membrane injuries.

This invention discloses the application of an artemisia annua compound as the sole active ingredient in the preparation of topical formulations for the prevention or improvement of blackheads and acne. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. This invention also discloses a topical formulation for the prevention or improvement of blackheads and acne, the sole active ingredient of which is an artemisia annua compound. Based on 100% of the total mass of the topical formulation, the artemisia annua compound accounts for 0.1% to 0.22%, and is composed of the following raw materials in parts by mass: camphene 4 to 10 parts, artemisinin 1 part. The artemisia annua compound of this invention can significantly reduce the formation of squalene peroxide in a UVA-induced squalene photo-oxidation model, and shows stronger inhibition against Malassezia and less impact on Staphylococcus epidermidis in a Malassezia vs. symbiotic bacteria comparison model.

The invention discloses a lipase mutant and application thereof, and belongs to the field of gene engineering and enzyme engineering. On the basis of staphylococcus epidermidis-derived lipase, two single-point mutants A86K and Q125L and a two-point combined mutant A86K-Q125L are obtained through screening, the reaction specificity to isoamyl alcohol is improved by the mutants, the reaction specificity to ethanol is reduced by the mutants, and the three mutants can catalyze esterification reaction under the condition of high water activity. The mutants provided by the invention are more suitable for industrial production than natural lipase, especially in the field of fusel oil removal of Baijiu, and have very huge application prospects and industrial values.

The invention discloses a preparation method of torreya grandis hydrolat and application of the torreya grandis hydrolat in acne improvement, and belongs to the field of plant extracts. The Chinese torreya hydrolat is obtained by performing steam distillation, condensation and oil-water separation on Chinese torreya aril to obtain a water phase and then performing filtration. The scavenging rates of DPPH and ABTS < + > free radicals are 85% and 90% or above respectively, and good oxidation resistance is achieved. Besides, antibacterial tests show that the Chinese torreya hydrolat can remarkably inhibit proliferation of propionibacterium acnes, has little influence on staphylococcus epidermidis and can be applied to cosmetics for improving acnes.

The invention belongs to biomedical materials, and particularly relates to an antibacterial and anticoagulant coating material for a blood contact material and application of the antibacterial and anticoagulant coating material. The antibacterial and anticoagulant coating material comprises copper tetracarboxyl phenyl porphyrin loaded with an anticoagulant and water-soluble polysaccharide. A coating formed on the basis of the antibacterial and anticoagulant coating material can achieve antibacterial and anticoagulant effects at the same time, for example, the bacteriostasis rate on escherichia coli and the bacteriostasis rate on staphylococcus epidermidis can reach 90%; in addition, the activated part thromboplastin time, prothrombin time and thrombin time of the coating are obviously higher than those of an untreated blood contact material; and the hemolysis rate is lower than 5%.

The invention provides an application of a small molecule compound in preparation of antibacterial drugs. The small molecule compound is T-2307 or 0199-0032, and the T-2307 and the optimized analogue 0199-0032 of the T-2307, the antibacterial spectrum is wide, and the pertinence is strong. The T-2307 has obvious antibacterial activity on gram-positive bacteria (including staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis and enterococcus faecium), and has a certain inhibition effect on gram-negative bacteria such as escherichia coli and the like; the antibacterial activity of the T-2307 on gram-positive bacteria is inherited by the T-2307, the broad-spectrum antibacterial effect on gram-negative bacteria is further widened, the limitation of the T-2307 on the gram-negative bacteria is made up, and the T-2307 can be applied to preparation of broad-spectrum antibacterial drugs.

This invention discloses the application of dermal ethylsin in the preparation of antibacterial and repairing cosmetics or pharmaceuticals, and compositions containing it. Dermal ethylsin can inhibit Propionibacterium acnes, Staphylococcus aureus, and / or Staphylococcus epidermidis, and can promote keratinocyte migration and / or upregulate the expression of repair-related genes. Cytotoxicity tests show that dermal ethylsin has good safety. Human efficacy evaluation shows that serums containing dermal ethylsin can significantly reduce inflammatory papules, improve skin redness, and improve barrier function. Therefore, dermal ethylsin can be used as a dual-action antibacterial and repairing active agent in cosmetics or pharmaceuticals for acne skin care, sensitive skin repair, and / or post-medical aesthetic repair. Dermal ethylsin is derived from *Gnaphalium affine*, has good safety, and is non-irritating to the skin.