The invention provides a primer group, kit and method for identifying staphylococcus in an environment at an in-species level, and belongs to the technical field of molecular biological detection. The primer group comprises primers F1 and R1 for amplifying staphylococcus aureus, primers F2 and R2 for amplifying staphylococcus warneri, primers F3 and R3 for amplifying staphylococcus epidermidis, primers F4 and R4 for amplifying staphylococcus hominis, primers F5 and R5 for amplifying staphylococcus capitis, and primers F6 and R6 for amplifying staphylococcus haemolyticus; and the nucleotide sequences of the primers F1, R1, F2, R2, F3, R3, F4, R4, F5, R5, F6 and R6 are respectively as shown in SEQ ID No. 1 to SEQ ID No. 12. The primer group, kit and method are easy to operate and high in sensitivity, and can complete the detection of environmental staphylococcus in a short time; and the method is low in cost, can identify various staphylococcus species, and is convenient for popularization and application.