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Applications of corn growth promoting rhizobacteria in promoting growth of plants

A technology of rhizosphere growth-promoting bacteria and root growth, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve the problems of rare corn growth-promoting bacteria public reports and other issues

Active Publication Date: 2021-04-20
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is an inevitable choice for the current development of ecological agriculture to explore the beneficial growth-promoting bacteria isolated from sandy fluvo-aquic soil, while reducing the use of pesticides and fertilizers and protecting the environment. public reports

Method used

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  • Applications of corn growth promoting rhizobacteria in promoting growth of plants
  • Applications of corn growth promoting rhizobacteria in promoting growth of plants
  • Applications of corn growth promoting rhizobacteria in promoting growth of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Activation of maize rhizosphere growth-promoting bacteria:

[0042] Bacterial strains are purchased from the General Microbiology Center of China Microbial Strain Collection Management Committee, and the preservation number of this bacterial strain is CGMCC 1.10840; for the convenience of recording, it is recorded as bacterial strain L60 in the present invention, and is called corn rhizosphere growth-promoting bacteria L60;

[0043] The corn rhizosphere growth-promoting bacteria L60 strain obtained above was activated on LB solid medium, placed upside down in a constant temperature incubator at 30°C for 24 hours, and activated for use.

[0044] LB medium: 5g of yeast extract, 10g of tryptone, 10g of sodium chloride, 1000ml of distilled water, 15g of solid plus agar powder, sterilized at 121°C for 20 minutes.

Embodiment 2

[0046] Identification of maize rhizosphere growth-promoting bacteria L60:

[0047] Observe the morphological characteristics of the above-mentioned activated corn rhizosphere growth-promoting bacteria L60 on the plate, such as figure 1 As shown, the colony morphology is as follows: milky white, opaque, raised, with neat edges, moist surface, and spherical shape.

[0048] 16S rDNA was identified as Staphylococcus haemolyticus.

Embodiment 3

[0050] Aerobic experiment:

[0051] Pour the freshly sterilized LB solid medium into 2 / 3 of the test tube, pick up the partially purified strain L60 with a sterile inoculation needle, and inoculate it to the bottom of the test tube. The test tubes were placed in a constant temperature incubator at 30°C for 3-7 days, and the results were observed. If the strain grows on the surface of the test tube medium, it is aerobic; if it grows along the puncture line, it is anaerobic; if both, it is facultative anaerobic.

[0052] The observation results showed that the growth of the strain L60 was distributed on the medium surface and the puncture line, and it was a facultative anaerobic bacteria.

[0053]Gram staining of strain L60 was positive, and the aerobic test was facultative anaerobic.

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Abstract

The invention relates to the technical field of microbial application, and specifically discloses applications of corn rhizosphere growth promoting bacteria in promoting growth of plants. The corn rhizosphere growth promoting bacteria are staphylococcus haemolyticus and can be used for promoting the growth of corns and arabidopis thaliana. Volatile substances produced by the strains can promote the growth of the corns and arabidopis thaliana, especially have efficient promotion effects on the roots of the corns and arabidopis thaliana.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to the application of a corn rhizosphere growth-promoting bacterium in promoting plant growth. Background technique [0002] Sandy fluvo-aquic soil is one of the main types of fluvo-aquic soil, and the area of ​​sandy fluvo-aquic soil in Henan Province is about 600,000 hm 2 , Accounting for 89.8% of the province's sandy land area, its parent material is mainly sandy sediments from the crevasses and flood mainstreams of the Yellow River, most of which are distributed in the east and northeast of Henan in the Huanghuai alluvial plain. Although this area has the advantages of light, temperature, water and other resources, due to the coarse soil texture, high sand content and loose structure, it is easy to cultivate and stand seedlings, and at the same time, it has low nutrient content, small water capacity and poor water holding capacity. , strong permeability, easy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01N37/02A01N27/00A01P21/00C12R1/44
Inventor 胡筑兵王磊郭铁城徐惠
Owner HENAN UNIVERSITY
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