A kit for detecting hemolytic staphylococcus

Abstract

The invention provides a kit for detecting staphylococcus haemolyticus. The kit comprises guide RNA specifically targeting staphylococcus haemolyticus rpoB gene, an amplification primer pair, hydration TwistAmp basic kit reaction drying balls, LbCas12a protein, a single-chain DNA probe (ssDNA), a Ribonuclease Inhibitor and a buffer solution. When the kit is used for detecting the staphylococcus haemolyticus, high detection specificity is achieved, and the staphylococcus haemolyticus can be distinguished from other staphylococcus such as staphylococcus aureus, staphylococcus hominis, staphylococcus warneri, staphylococcus capitis and staphylococcus epidermidis. Meanwhile, when the RNA sequence is used to perform detection, detection time is about 1 hour, a PCR instrument is not needed, equipment requirements are evidently lower than those of a traditional bacterial culture method or a PCR sequencing method, and a high clinical practical value is achieved.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for detection of hemolytic staphylococcus, comprising a guide RNA specifically targeting the rpoB gene of hemolytic staphylococcus, a pair of RPA amplification primers, and a hydrated TwistAmp basickit for reaction drying Ball, LbCas12a protein, single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor and buffer. This kit can be used for rapid detection of hemolytic staphylococcus. Background technique [0002] Staphylococcus hemolyticus is a common bacterial group among Gram-positive bacteria that infects humans, which brings a huge burden to patients and society. The traditional detection method is to carry out bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish hemolytic staphylococcus from other staphylococci due to the lack of specific primers, so joint generation sequencing is required. I...

AI Technical Summary

Problems solved by technology

The traditional detection method is to carry out bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish hemolytic staphylococcus from other staphylococci due to the lack of specific primers, so joint generation sequencing is required. Identification, high cost, takes several days to get results

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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