The invention discloses an application of a Chinese medicinal herb layer feed additive. The Chinese medicinal herb layer feed additive and a layer basal diet are mixed together according to a weight ratio of (1-10):(90-99) to form a layer feed for feeding layers, wherein the additive comprises the following Chinese medicinal herb components: hawthorn, astragalus root, sealwort, Chinese angelica root, herba epimedii, codonopsis pilosula, curcuma aromatic, medicated leaven, folium artemisiae argyi, bark of eucommia, spina date seeds, serissa serissoides, purslane, glossy privet fruits, nettle, mulberry, polygonatum odoratum, phyllanthus emblica, cyrtomium fortune, carthamus tinctorius, cassia seeds, eclipta alba, herba cistanches, broom cypress fruits, geum urbanum, herba aristolochiae, mint, herba dendrobii, pine needle meal and licorice. The additive applied to the layer feed can directly kill and inhibit bacteria and adjust the immune function of an organism, has a non-specific antibacterial effect, does not generate dependence, and can improve the production performance of layers, improve the laying rate and egg quality, and reduce the cholesterol level of eggs.