Plant-source skin-tendering oral liquid and preparation method thereof

Abstract

The invention relates to the technical field of oral liquid, in particular to plant-source skin-tendering oral liquid and a preparation method thereof, and the plant-source skin-tendering oral liquid comprises the following raw materials: an inclusion composition, plant-source composition fermentation powder, pleurotus citrinopileatus concentrated powder, cubilose peptide, sodium hyaluronate, haematococcus pluvialis powder and distilled water. According to the invention, the emblic leafflower fruit extract is encapsulated by using the abelmoschus esculentus pectin extract-gelatin complex, so that the emblic leafflower fruit extract is tightly wrapped in the inclusion composition, and the inclusion composition is beneficial to retaining the active ingredients of the emblic leafflower fruit extract, is more beneficial to reducing the generation of AGEs, and covers the bitter taste of the emblic leafflower fruit extract; the drinking taste of the plant source skin tendering oral liquid is improved; according to the present invention, the plant source composition is subjected to fermentation treatment, such that the synthesis and the accumulation of the rutin, the quercetin and the kaempferol in the plant source composition can be promoted, such that the plant source composition fermentation powder obtained after the fermentation treatment can easily reduce the generation of AGEs, and can enhance the anti-saccharification and anti-aging effects.

Description

Technical Field

[0001] The present invention relates to the technical field of oral liquids, and more particularly to a plant-derived skin rejuvenation oral liquid and a preparation method thereof. Background Art

[0002] The oral solution absorbs the technological characteristics of Chinese medicine injection and is obtained by further refining, concentrating, filling and sterilizing the decoction. It has the advantages of small dosage, rapid absorption, stable quality, convenient carrying and taking, and easy storage. It is especially suitable for industrial production.

[0003] Glycation refers to a slow, non-enzymatic reaction between the amino group of a protein and the carbonyl group of a reducing sugar. The reaction first forms an unstable and reversible Schiff base, which then undergoes molecular rearrangement to eventually form a complex, stable covalent polymer with diverse structures and irreversibility, called advanced glycation end products (AGEs). AGEs molecules can cross-link to form macromolecules that accumulate in various tissues and organs, including the brain and skin. These substances can cause collagen and elastin to lose their elasticity, causing the skin to become rough, loose, and show signs of aging. Therefore, in order to maintain the elasticity and luster of the skin and achieve anti-aging and skin rejuvenation effects, it is necessary to reduce the generation and accumulation of AGEs in the human body.

[0004] At present, AGEs inhibitors with anti-glycation effects are usually used to inhibit the formation and accumulation of AGEs. AGEs inhibitors are mainly divided into two categories: synthetic and natural. Synthetic inhibitors such as aminoguanidine, quinine, thiazolidinediones and metformin have the effect of inhibiting AGEs, but these substances still have many side effects and safety hazards. Plant extracts are natural inhibitors, which have been proven to have the effect of inhibiting AGEs and have stronger inhibitory ability than synthetic inhibitors. They are the best alternative to synthetic inhibitors.

[0005] Studies have shown that the extract of emblica fruit has a significant anti-glycation effect and can be used as a natural inhibitor of AGEs. The flavonoids and phenolic acid components in it have certain antioxidant properties, which can help resist the oxidative stress caused by glycation reactions and inhibit the formation of AGEs. However, due to the presence of a large number of easily oxidized active ingredients, the extract of emblica fruit may be degraded by factors such as light and temperature during storage and processing, affecting its efficacy. In addition, the extract of emblica fruit contains a large number of tannin components, which give the emblica fruit a bitter taste, which affects the taste when added to the anti-sugar oral solution, making it difficult for some consumers to accept it. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a plant-derived skin rejuvenation oral liquid which uses plant-derived extracts as raw materials, has anti-glycation efficacy, and plays the role of slowing down aging and protecting the skin and beauty, and a preparation method thereof.

[0007] A plant-derived skin rejuvenation oral liquid, comprising the following raw materials: 5-20 parts by weight of the inclusion composition, 3-24 parts by weight of fermented plant composition powder, 1-6 parts by weight of Pleurotus eryngii concentrated powder, 0.3-2 parts by weight of bird's nest peptide, 1-4 parts by weight of sodium hyaluronate, 0.5-2.5 parts by weight of Haematococcus pluvialis powder and 300-600 parts by weight of distilled water; The raw materials of the inclusion composition include: an emblica extract emulsion and an okra pectin extract-gelatin complex, wherein the mass ratio of the emblica extract emulsion to the okra pectin extract-gelatin complex is (1-5): (3-10); The raw materials of the emblica extract emulsion include: an emblica extract solution and an oil, wherein the volume ratio of the emblica extract solution to the oil is (1-2):(2-4), the emblica extract solution is prepared by mixing the emblica extract with distilled water, and the concentration of the emblica extract solution is 20-30 mg / mL; The okra pectin extract-gelatin complex is prepared by stirring a 1-3% complex dispersion and adjusting the pH value to 2.2-3.8, wherein the 1-3% complex dispersion is prepared by mixing the okra pectin extract and gelatin in a mass ratio of (1-4): (1-3), and then mixing with distilled water; The raw materials of the plant-derived composition fermentation powder include: a plant-derived composition enzymatic solution and a composite fermentation bacterial agent, wherein the added amount of the composite fermentation bacterial agent is 1 to 5% of the plant-derived composition enzymatic solution; The plant-based composition enzymatic hydrolysis solution is obtained by subjecting the plant-based composition solution to enzymatic hydrolysis reactions of pectinase and cellulase. The plant-based composition solution is prepared by mixing the plant-based composition and distilled water in a mass ratio of 1:(26-38). The raw materials of the plant-based composition include: 8-18 parts by weight of mulberry leaves, 3-15 parts by weight of buckwheat, 2-9 parts by weight of locust flowers, 5-16 parts by weight of honeysuckle, 10-30 parts by weight of apples and 6-24 parts by weight of grapes.

[0008] Furthermore, the oil is prepared by mixing rice bran oil and polyglycerol ricinoleate, wherein the volume fraction of polyglycerol ricinoleate is 5%.

[0009] A method for preparing a plant-derived skin rejuvenation oral liquid comprises the following steps: S1: Okra separation and extraction treatment, drying and grinding okra into okra powder, preparing okra powder into okra solution, and subjecting the solution to microwave extraction, deproteinization, centrifugal separation, dialysis and freeze drying to obtain okra pectin extract; S2: Preparation of okra pectin extract-gelatin complex, mixing okra pectin extract and gelatin according to a proportion, adding distilled water to prepare a complex dispersion, heating and stirring for a period of time, and adjusting the pH of the complex dispersion to 2.2-3.8 to obtain the okra pectin extract-gelatin complex; S3: Preparation and inclusion processing of emblica extract, the emblica is crushed and mixed with an ethanol aqueous solution, and the emblica extract is obtained by reflux extraction, suction filtration, decompression removal of ethanol, freeze drying and grinding, the emblica extract is mixed with distilled water and oil to obtain an emblica extract emulsion, the emblica extract emulsion and okra pectin extract-gelatin complex are mixed, and the inclusion composition is obtained by high-speed homogenization and freeze drying; S4: fermentation treatment of the plant-derived composition, mulberry leaves, buckwheat, Sophora japonica, honeysuckle, apples and grapes are crushed and mixed to obtain the plant-derived composition, the plant-derived composition is mixed with distilled water, pectinase and cellulase are added to carry out enzymolysis reaction, and then a composite fermentation agent is added to carry out fermentation reaction. After the fermentation is completed, centrifugation is carried out, the supernatant is taken, vacuum concentration is carried out and spray drying is carried out to obtain fermentation powder of the plant-derived composition; S5: Preparation of plant-derived skin rejuvenation oral liquid: the inclusion composition, plant-derived composition fermentation powder, Pleurotus eryngii concentrated powder, bird's nest peptide, sodium hyaluronate, Haematococcus pluvialis powder and distilled water are stirred and mixed, and the plant-derived skin rejuvenation oral liquid is obtained through filtering, filling and sterilization.

[0010] Further, step S1: separation and extraction of okra, specifically comprising the following steps: S1.1: Wash the okra, cut it into pieces, freeze-dry it and grind it to obtain okra powder; S1.2: Okra powder and distilled water are mixed in a mass ratio of 1: (10-50) to obtain an okra solution, and the okra solution is subjected to microwave extraction for 6-15 minutes at a temperature of 40-60°C and a microwave power of 500-900W. Subsequently, the microwave-extracted solution is centrifuged at 6000-8000rpm for 6-12 minutes, and the supernatant is collected. The microwave extraction, centrifugation and supernatant are repeatedly performed on the remaining solution after the supernatant is collected. The above operation is repeated 2-4 times, and the supernatants obtained each time are combined to obtain an okra extract; S1.3: The okra extract is concentrated under reduced pressure to 1 / 4 to 1 / 3 of the original volume, and the concentrated okra extract is mixed with an organic solvent mixture according to a volume ratio of (2 to 4): 1, and stirred for 20 to 30 minutes. Subsequently, the mixed solution of the concentrated okra extract and the organic solvent mixture is centrifuged at 6000 to 8000 rpm for 5 to 10 minutes, the upper liquid is taken, the protein precipitate and the lower organic layer are discarded, and the above-mentioned organic solvent mixture mixing, stirring, centrifugal separation and upper liquid collection operations are repeated for the upper liquid until no protein appears, thereby obtaining a deproteinized extract; S1.4: Mix the deproteinized extract and anhydrous ethanol in a volume ratio of 1:(2-4), store at 3-5°C for 10-16 hours, and then centrifuge. Put the precipitate obtained after centrifugation into a dialysis bag and dialyze it for 24-48 hours. The molecular weight cutoff of the dialysis bag is 3500Da. Then, freeze-dry to obtain the okra pectin extract.

[0011] Furthermore, in step S1.3, the organic solvent mixture is prepared by mixing chloroform and n-butanol in a volume ratio of 4:1.

[0012] Further, step S2: preparation of okra pectin extract-gelatin complex specifically comprises the following steps: S2.1: Mix okra pectin extract and gelatin in a mass ratio of (1-4):(1-3), and then add distilled water to prepare a composite dispersion with a mass fraction of 1-3%; S2.2: The complex dispersion is magnetically stirred at 40-50° C. for 2-3 h, and then a 1 mol / L hydrochloric acid solution is added to adjust the pH of the complex dispersion to 2.2-3.8, and the mixture is slowly stirred for 5-15 min to obtain an okra pectin extract-gelatin complex.

[0013] Further, step S3: preparation and inclusion processing of Phyllanthus emblica extract, specifically comprises the following steps: S3.1: crush the emblica buds and pass through a 150-250 mesh sieve to obtain emblica buds coarse powder; S3.2: The emblica powder and 70-80% ethanol aqueous solution are mixed in a mass ratio of 1: (80-120), and the mixed solution is refluxed for 2-3 times at 70-75°C in a nitrogen atmosphere, and the time of each reflux extraction is 1-2 hours. The extracts obtained after each reflux extraction are combined, and the combined extracts are cooled to room temperature, filtered, decompressed to remove ethanol, freeze-dried and ground to obtain the emblica extract; S3.3: adding the emblica extract to distilled water to prepare an emblica extract solution with a concentration of 20-30 mg / mL, mixing the emblica extract solution with an oil liquid in a volume ratio of (1-2):(2-4), and subjecting the mixture of the emblica extract solution and the oil liquid to high-speed shearing using a high-speed shear emulsifier at a shear speed of 22000-28000 rpm and a shearing time of 3-5 min to obtain an emblica extract emulsion; S3.4: The emblica extract emulsion and the okra pectin extract-gelatin complex are mixed in a volume ratio of (1-5): (3-10), and the mixed solution of the emblica extract emulsion and the okra pectin extract-gelatin complex is homogenized at high speed using a homogenizer for 3-5 minutes, followed by freeze-drying to obtain an inclusion composition.

[0014] Further, step S4: fermentation treatment of the plant-derived composition specifically comprises the following steps: S4.1: 8 to 18 parts by weight of mulberry leaves, 3 to 15 parts by weight of buckwheat, 2 to 9 parts by weight of Sophora japonica, 5 to 16 parts by weight of honeysuckle, 10 to 30 parts by weight of apples and 6 to 24 parts by weight of grapes are respectively crushed into powders and passed through a 200 to 300 mesh sieve, and all the powders obtained after sieving are mixed to obtain a plant-derived composition; S4.2: Mix the plant-derived composition and distilled water in a mass ratio of 1:(26-38) and then add sodium bicarbonate to adjust the pH of the mixed solution of the plant-derived composition and distilled water to 4.5-5.4 to obtain a plant-derived composition solution; S4.3: adding pectinase and cellulase to the plant-derived composition solution, and performing enzymolysis reaction at 30-50° C. for 2-4 hours. After the enzymolysis is completed, adding sodium bicarbonate, and adjusting the pH of the plant-derived composition solution after enzymolysis to 5.5-6.0, to obtain an enzymolysis solution of the plant-derived composition; S4.4: stirring the enzymatic hydrolysis solution of the plant-derived composition at 80-100° C. for 10-30 minutes, then cooling to 25-35° C., adding a composite fermentation agent to the enzymatic hydrolysis solution of the plant-derived composition, wherein the amount of the composite fermentation agent added is 1-5% of the enzymatic hydrolysis solution of the plant-derived composition, and fermenting for 2-5 days to obtain a fermentation solution of the plant-derived composition; S4.5: Centrifuge the fermented solution of the plant-derived composition for 5 to 10 minutes, take the supernatant, and perform vacuum low-temperature concentration and spray drying on the supernatant. The temperature of the vacuum low-temperature concentration is 45 to 60° C. to obtain the fermented powder of the plant-derived composition.

[0015] Furthermore, in step S4.3, the added amounts of pectinase and cellulase are both 2-6% of the plant-based composition solution, the enzymatic activity of cellulase is 5000-20000 U / g, and the enzymatic activity of pectinase is 5000-20000 U / g.

[0016] Further, in step S4.4, the composite fermentation bacteria agent is composed of Lactobacillus plantarum, lactic acid bacteria, Lactobacillus casei, Lactobacillus acidophilus, Bifidobacterium bifidum and Lactococcus lactis in a mass ratio of 1:1:1:1:1:1:1, and the effective live bacteria count of Lactobacillus plantarum is ≥50 million cuf / g, the effective live bacteria count of lactic acid bacteria is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus casei is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus acidophilus is ≥50 million cuf / g, the effective live bacteria count of Bifidobacterium bifidum is ≥50 million cuf / g, and the effective live bacteria count of Lactococcus lactis is ≥50 million cuf / g.

[0017] The present invention has the following advantages: 1. In the present invention, the okra pectin extract obtained after the okra separation and extraction treatment is a polysaccharide, and multiple hydroxyl groups and other polar groups exist in its molecules, which can be combined with the amino acid residues of gelatin through hydrogen bond interaction, and the okra pectin extract and gelatin further form a stable complex through the electrostatic attraction under acidic conditions. The obtained okra pectin extract-gelatin complex can form a strong gel network under strong electrostatic interaction. When used as a carrier, it can incorporate all water molecules and water-soluble molecules into its gel network structure, effectively lock in moisture and dissolved components, protect the internal active substances from damage, and keep its functions intact; and the okra pectin extract component in the okra pectin extract-gelatin complex has anti-glycation effect, can inhibit the activity of AGEs receptors, reduce the generation of AGEs, and play a role in slowing down aging and skin care.

[0018] 2. In the present invention, the okra pectin extract-gelatin complex is used to encapsulate the emblica extract. The okra pectin extract-gelatin complex tightly wraps the emblica extract, effectively preventing the external environment from directly contacting and destroying the emblica extract, and maintaining the internal stability and activity of the emblica extract. The obtained inclusion composition retains the active ingredients of the emblica extract, which is beneficial to ensuring the anti-glycation effect of the emblica extract and reducing the formation of AGEs. In addition, the emblica extract encapsulated in the inclusion composition can effectively mask the bitter taste in the emblica extract, enhance the edible taste, and improve consumer acceptance.

[0019] 3. In the present invention, the plant-based composition is fermented and the metabolism of microorganisms is utilized to promote the synthesis and accumulation of rutin, quercetin and kaempferol components in the plant-based composition, thereby increasing the effective content of rutin, quercetin and kaempferol in the plant-based composition, so that the fermented powder of the plant-based composition obtained after the fermentation treatment is more conducive to reducing the formation of AGEs and enhancing the anti-glycation and anti-aging effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The flowchart of the preparation method of the plant-derived skin rejuvenation oral liquid in the embodiment of the present invention is shown in FIG.

[0021] Figure 2 This is a graph showing the loss rate of gallic acid content of the inclusion composition in Example 1 of the present invention and the mixture of the emblica extract emulsion and the okra pectin extract-gelatin complex in Comparative Example 1 before and after standing. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1 A method for preparing a plant-derived skin rejuvenation oral liquid, such as Figure 1 As shown, the specific steps include: S1: Okra separation and extraction, S1.1: Wash the okra, cut it into pieces, freeze-dry it and grind it to obtain okra powder; S1.2: Okra powder and distilled water were mixed in a mass ratio of 1:30 to obtain an okra solution. The okra solution was subjected to microwave extraction for 15 min at a temperature of 60°C and a microwave power of 900 W. Subsequently, the microwave-extracted solution was centrifuged at 8000 rpm for 12 min, and the supernatant was collected. The microwave extraction, centrifugation and supernatant were repeatedly performed on the remaining solution after the supernatant was collected. The above operation was repeated 4 times, and the supernatants obtained each time were combined to obtain an okra extract. S1.3: The okra extract is concentrated under reduced pressure to 1 / 3 of the original volume, and the concentrated okra extract is mixed with an organic solvent mixture at a volume ratio of 4:1, and stirred for 30 minutes, wherein the organic solvent mixture is prepared by chloroform and n-butanol at a volume ratio of 4:1, and then the mixed solution of the concentrated okra extract and the organic solvent mixture is centrifuged at 8000 rpm for 10 minutes, the upper liquid is taken, the protein precipitate and the lower organic layer are discarded, and the above-mentioned organic solvent mixture mixing, stirring, centrifugal separation and upper liquid collection operations are repeated for the upper liquid until no protein appears, thereby obtaining a deproteinized extract; S1.4: The deproteinized extract and anhydrous ethanol were mixed in a volume ratio of 1:4, and the mixture was allowed to stand at 4°C for 16 hours, followed by centrifugation. The precipitate obtained after the centrifugation was placed in a dialysis bag and dialyzed for 48 hours. The molecular weight cutoff of the dialysis bag was 3500Da, and then freeze-dried to obtain the okra pectin extract. S2: Preparation of okra pectin extract-gelatin complex, S2.1: okra pectin extract and gelatin were mixed in a mass ratio of 3:1, and then distilled water was added to prepare a composite dispersion with a mass fraction of 3%; S2.2: The composite dispersion was magnetically stirred at 50°C for 3 h, and then a 1 mol / L hydrochloric acid solution was added to adjust the pH of the composite dispersion to 3.2, and the mixture was slowly stirred for 15 min to obtain an okra pectin extract-gelatin complex; S3: Preparation and inclusion processing of Phyllanthus emblica extract, S3.1: crush the emblica buds and pass through a 250-mesh sieve to obtain emblica buds coarse powder; S3.2: The emblica powder and 75% ethanol aqueous solution were mixed in a mass ratio of 1:100, and the mixed solution was refluxed and extracted three times at 75°C in a nitrogen atmosphere, each reflux extraction time was 2 hours, and the extracts obtained after each reflux extraction were combined. After the combined extracts were cooled to room temperature, they were filtered, ethanol was removed under reduced pressure, freeze-dried and ground to obtain the emblica extract; S3.3: adding the emblica extract to distilled water to prepare an emblica extract solution with a concentration of 30 mg / mL, mixing the emblica extract solution with an oil liquid at a volume ratio of 1:4, wherein the oil liquid is prepared by mixing rice bran oil and polyglycerol ricinoleate, and the volume fraction of polyglycerol ricinoleate in the oil liquid is 5%, and subjecting the mixture of the emblica extract solution and the oil liquid to high-speed shearing by a high-speed shearing emulsifier, at a shearing speed of 24000 rpm and a shearing time of 5 min, to obtain an emblica extract emulsion; S3.4: The emblica extract emulsion and the okra pectin extract-gelatin complex are mixed in a volume ratio of 3:8, and the mixed solution of the emblica extract emulsion and the okra pectin extract-gelatin complex is homogenized at high speed by a homogenizer for 5 minutes, followed by freeze drying to obtain an inclusion composition; S4: Fermentation treatment of plant-derived composition, S4.1: 18 parts by weight of mulberry leaves, 15 parts by weight of buckwheat, 9 parts by weight of Sophora japonica, 16 parts by weight of honeysuckle, 30 parts by weight of apples and 24 parts by weight of grapes are respectively crushed into powders and passed through a 300-mesh sieve, and all the powders obtained after sieving are mixed to obtain a plant-derived composition; S4.2: The plant-derived composition and distilled water are mixed evenly at a mass ratio of 1:36, and sodium bicarbonate is added to adjust the pH of the mixed solution of the plant-derived composition and distilled water to 5.0 to obtain a plant-derived composition solution; S4.3: Add pectinase and cellulase to the plant-derived composition solution, the amount of pectinase and cellulase added is 6% of the plant-derived composition solution, stir evenly, and perform enzymolysis reaction at 40° C. for 4 hours. After the enzymolysis is completed, add sodium bicarbonate and adjust the pH of the plant-derived composition solution after enzymolysis to 6.0 to obtain an enzymolysis solution of the plant-derived composition; S4.4: Stir the enzymatic hydrolysis solution of the plant-derived composition at 100°C for 10 minutes, then cool it to 35°C, add a composite fermentation agent to the enzymatic hydrolysis solution of the plant-derived composition, the amount of the composite fermentation agent added is 3% of the enzymatic hydrolysis solution of the plant-derived composition, and ferment for 5 days to obtain a fermentation solution of the plant-derived composition, wherein the composite fermentation agent is composed of Lactobacillus plantarum, lactic acid bacteria, Lactobacillus casei, Lactobacillus acidophilus, Bifidobacterium bifidum and Lactococcus lactis in a mass ratio of 1:1:1:1:1:1, and the effective live bacteria count of Lactobacillus plantarum is ≥50 million cuf / g, the effective live bacteria count of lactic acid bacteria is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus casei is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus acidophilus is ≥50 million cuf / g, the effective live bacteria count of Bifidobacterium bifidum is ≥50 million cuf / g, and the effective live bacteria count of Lactococcus lactis is ≥50 million cuf / g; S4.5: centrifuge the fermented solution of the plant-derived composition for 10 minutes, take the supernatant, and perform vacuum low-temperature concentration and spray drying on the supernatant. The vacuum low-temperature concentration temperature is 50° C. to obtain the fermented powder of the plant-derived composition; S5: Preparation of plant-derived skin rejuvenation oral solution, 20 parts by weight of the inclusion composition, 24 parts by weight of fermented powder of the plant-based composition, 6 parts by weight of Pleurotus eryngii concentrated powder, 2 parts by weight of bird's nest peptide, 4 parts by weight of sodium hyaluronate, 2.5 parts by weight of Haematococcus pluvialis powder and 600 parts by weight of distilled water are stirred and mixed, and then filtered, filled and sterilized to obtain a plant-based skin rejuvenation oral liquid.

[0024] Example 2 A method for preparing a plant-derived skin rejuvenation oral liquid, such as Figure 1 As shown, the specific steps include: S1: Okra separation and extraction, S1.1: Wash the okra, cut it into pieces, freeze-dry it and grind it to obtain okra powder; S1.2: Okra powder and distilled water were mixed in a mass ratio of 1:30 to obtain an okra solution. The okra solution was subjected to microwave extraction at a temperature of 40°C and a microwave power of 900 W for 10 min. Subsequently, the microwave-extracted solution was centrifuged at 8000 rpm for 6 min, and the supernatant was collected. The microwave extraction, centrifugation and supernatant were repeatedly performed on the remaining solution after the supernatant was collected. The above operation was repeated 4 times, and the supernatants obtained each time were combined to obtain an okra extract. S1.3: The okra extract is concentrated under reduced pressure to 1 / 3 of the original volume, and the concentrated okra extract is mixed with an organic solvent mixture at a volume ratio of 4:1, and stirred for 20 minutes, wherein the organic solvent mixture is prepared by chloroform and n-butanol at a volume ratio of 4:1, and then the mixed solution of the concentrated okra extract and the organic solvent mixture is centrifuged at 8000 rpm for 6 minutes, the upper liquid is taken, the protein precipitate and the lower organic layer are discarded, and the above-mentioned organic solvent mixture mixing, stirring, centrifugal separation and upper liquid collection operations are repeated for the upper liquid until no protein appears, thereby obtaining a deproteinized extract; S1.4: The deproteinized extract and anhydrous ethanol were mixed in a volume ratio of 1:4, and the mixture was allowed to stand at 5°C for 12 hours, followed by centrifugation. The precipitate obtained after centrifugation was placed in a dialysis bag and dialyzed for 48 hours. The molecular weight cutoff of the dialysis bag was 3500Da, and then freeze-dried to obtain the okra pectin extract. S2: Preparation of okra pectin extract-gelatin complex, S2.1: okra pectin extract and gelatin were mixed in a mass ratio of 3:1, and then distilled water was added to prepare a composite dispersion with a mass fraction of 3%; S2.2: The composite dispersion was magnetically stirred at 40°C for 2 h, and then a 1 mol / L hydrochloric acid solution was added to adjust the pH of the composite dispersion to 3.2, and the mixture was slowly stirred for 15 min to obtain an okra pectin extract-gelatin complex; S3: Preparation and inclusion processing of Phyllanthus emblica extract, S3.1: crush the emblica buds and pass through a 250-mesh sieve to obtain emblica buds coarse powder; S3.2: The emblica powder and 75% ethanol aqueous solution were mixed in a mass ratio of 1:100, and the mixed solution was refluxed and extracted three times at 70°C in a nitrogen atmosphere, each reflux extraction time was 1 hour, and the extracts obtained after each reflux extraction were combined, and the combined extracts were cooled to room temperature, filtered, decompressed to remove ethanol, freeze-dried and ground to obtain the emblica extract; S3.3: adding the emblica extract to distilled water to prepare an emblica extract solution with a concentration of 30 mg / mL, mixing the emblica extract solution with an oil liquid at a volume ratio of 1:4, wherein the oil liquid is prepared by mixing rice bran oil and polyglycerol ricinoleate, and the volume fraction of polyglycerol ricinoleate in the oil liquid is 5%, and subjecting the mixture of the emblica extract solution and the oil liquid to high-speed shearing by a high-speed shearing emulsifier, at a shearing speed of 28000 rpm and a shearing time of 3 min, to obtain an emblica extract emulsion; S3.4: The emblica extract emulsion and the okra pectin extract-gelatin complex are mixed in a volume ratio of 3:8, and the mixed solution of the emblica extract emulsion and the okra pectin extract-gelatin complex is homogenized at high speed by a homogenizer for 3 minutes, and then freeze-dried to obtain an inclusion composition; S4: Fermentation treatment of plant-derived composition, S4.1: 18 parts by weight of mulberry leaves, 15 parts by weight of buckwheat, 9 parts by weight of Sophora japonica, 16 parts by weight of honeysuckle, 30 parts by weight of apples and 24 parts by weight of grapes are respectively crushed into powders and passed through a 300-mesh sieve, and all the powders obtained after sieving are mixed to obtain a plant-derived composition; S4.2: The plant-derived composition and distilled water are mixed evenly at a mass ratio of 1:36, and sodium bicarbonate is added to adjust the pH of the mixed solution of the plant-derived composition and distilled water to 5.0 to obtain a plant-derived composition solution; S4.3: Add pectinase and cellulase to the plant-derived composition solution, the amount of pectinase and cellulase added is 6% of the plant-derived composition solution, stir evenly, and perform enzymolysis reaction at 30° C. for 2 hours. After the enzymolysis is completed, add sodium bicarbonate and adjust the pH of the plant-derived composition solution after enzymolysis to 6.0 to obtain an enzymolysis solution of the plant-derived composition; S4.4: Stir the enzymatic hydrolysis solution of the plant-derived composition at 80°C for 30 minutes, then cool it to 30°C, add a composite fermentation agent to the enzymatic hydrolysis solution of the plant-derived composition, the amount of the composite fermentation agent added is 3% of the enzymatic hydrolysis solution of the plant-derived composition, and ferment for 5 days to obtain a fermentation solution of the plant-derived composition, wherein the composite fermentation agent is composed of Lactobacillus plantarum, lactic acid bacteria, Lactobacillus casei, Lactobacillus acidophilus, Bifidobacterium bifidum and Lactococcus lactis in a mass ratio of 1:1:1:1:1:1, and the effective live bacteria count of Lactobacillus plantarum is ≥50 million cuf / g, the effective live bacteria count of lactic acid bacteria is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus casei is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus acidophilus is ≥50 million cuf / g, the effective live bacteria count of Bifidobacterium bifidum is ≥50 million cuf / g, and the effective live bacteria count of Lactococcus lactis is ≥50 million cuf / g; S4.5: centrifuge the fermented solution of the plant-derived composition for 10 minutes, take the supernatant, and perform vacuum low-temperature concentration and spray drying on the supernatant. The vacuum low-temperature concentration temperature is 50° C. to obtain the fermented powder of the plant-derived composition; S5: Preparation of plant-derived skin rejuvenation oral solution, 20 parts by weight of the inclusion composition, 24 parts by weight of fermented powder of the plant-based composition, 6 parts by weight of Pleurotus eryngii concentrated powder, 2 parts by weight of bird's nest peptide, 4 parts by weight of sodium hyaluronate, 2.5 parts by weight of Haematococcus pluvialis powder and 600 parts by weight of distilled water are stirred and mixed, and then filtered, filled and sterilized to obtain a plant-based skin rejuvenation oral liquid.

[0025] Example 3 A method for preparing a plant-derived skin rejuvenation oral liquid, such as Figure 1 As shown, the specific steps include: S1: Okra separation and extraction, S1.1: Wash the okra, cut it into pieces, freeze-dry it and grind it to obtain okra powder; S1.2: Okra powder and distilled water were mixed in a mass ratio of 1:10 to obtain an okra solution. The okra solution was subjected to microwave extraction for 15 min at a temperature of 60°C and a microwave power of 900 W. Subsequently, the microwave-extracted solution was centrifuged at 8000 rpm for 12 min, and the supernatant was collected. The microwave extraction, centrifugation and supernatant were repeatedly performed on the remaining solution after the supernatant was collected. The above operation was repeated 4 times, and the supernatants obtained each time were combined to obtain an okra extract. S1.3: The okra extract is concentrated under reduced pressure to 1 / 4 of the original volume, and the concentrated okra extract is mixed with an organic solvent mixture at a volume ratio of 2:1, and stirred for 30 minutes, wherein the organic solvent mixture is prepared by chloroform and n-butanol at a volume ratio of 4:1. Subsequently, the mixed solution of the concentrated okra extract and the organic solvent mixture is centrifuged at 8000 rpm for 10 minutes, the upper liquid is taken, the protein precipitate and the lower organic layer are discarded, and the above-mentioned organic solvent mixture mixing, stirring, centrifugal separation and upper liquid collection operations are repeated on the upper liquid until no protein appears, thereby obtaining a deproteinized extract; S1.4: The deproteinized extract and anhydrous ethanol were mixed in a volume ratio of 1:3, and the mixture was allowed to stand at 4°C for 16 hours, followed by centrifugation. The precipitate obtained after centrifugation was placed in a dialysis bag and dialyzed for 48 hours. The molecular weight cutoff of the dialysis bag was 3500Da, and then freeze-dried to obtain the okra pectin extract. S2: Preparation of okra pectin extract-gelatin complex, S2.1: okra pectin extract and gelatin were mixed in a mass ratio of 3:2, and then distilled water was added to prepare a composite dispersion with a mass fraction of 3%; S2.2: The composite dispersion was magnetically stirred at 50°C for 3 h, and then a 1 mol / L hydrochloric acid solution was added to adjust the pH of the composite dispersion to 2.8, and the mixture was slowly stirred for 15 min to obtain an okra pectin extract-gelatin complex; S3: Preparation and inclusion processing of Phyllanthus emblica extract, S3.1: crush the emblica buds and pass through a 250-mesh sieve to obtain emblica buds coarse powder; S3.2: The emblica powder and 75% ethanol aqueous solution were mixed in a mass ratio of 1:80, and the mixed solution was refluxed for extraction for 3 times at 75°C in a nitrogen atmosphere, each reflux extraction time was 2 hours, and the extracts obtained after each reflux extraction were combined. After the combined extracts were cooled to room temperature, they were filtered, ethanol was removed under reduced pressure, freeze-dried and ground to obtain the emblica extract; S3.3: adding the emblica extract to distilled water to prepare an emblica extract solution with a concentration of 20 mg / mL, mixing the emblica extract solution with an oil liquid at a volume ratio of 1:3, wherein the oil liquid is prepared by mixing rice bran oil and polyglycerol ricinoleate, and the volume fraction of polyglycerol ricinoleate in the oil liquid is 5%, and subjecting the mixture of the emblica extract solution and the oil liquid to high-speed shearing by a high-speed shearing emulsifier, at a shearing speed of 24000 rpm and a shearing time of 5 min, to obtain an emblica extract emulsion; S3.4: The emblica extract emulsion and the okra pectin extract-gelatin complex are mixed in a volume ratio of 1:4, and the mixed solution of the emblica extract emulsion and the okra pectin extract-gelatin complex is homogenized at high speed by a homogenizer for 5 minutes, and then freeze-dried to obtain an inclusion composition; S4: Fermentation treatment of plant-derived composition, S4.1: 18 parts by weight of mulberry leaves, 15 parts by weight of buckwheat, 9 parts by weight of Sophora japonica, 16 parts by weight of honeysuckle, 30 parts by weight of apples and 24 parts by weight of grapes are respectively crushed into powders and passed through a 300-mesh sieve, and all the powders obtained after sieving are mixed to obtain a plant-derived composition; S4.2: The plant-derived composition and distilled water are mixed evenly at a mass ratio of 1:30, and sodium bicarbonate is added to adjust the pH of the mixed solution of the plant-derived composition and distilled water to 5.0 to obtain a plant-derived composition solution; S4.3: Add pectinase and cellulase to the plant-derived composition solution, the amount of pectinase and cellulase added is 3% of the plant-derived composition solution, stir evenly, and perform enzymolysis reaction at 40° C. for 4 hours. After the enzymolysis is completed, add sodium bicarbonate and adjust the pH of the plant-derived composition solution after enzymolysis to 6.0 to obtain an enzymolysis solution of the plant-derived composition; S4.4: Stir the enzymatic hydrolysis solution of the plant-derived composition at 100°C for 10 minutes, then cool it to 35°C, add a composite fermentation agent to the enzymatic hydrolysis solution of the plant-derived composition, the amount of the composite fermentation agent added is 5% of the enzymatic hydrolysis solution of the plant-derived composition, and ferment for 5 days to obtain a fermentation solution of the plant-derived composition, wherein the composite fermentation agent is composed of Lactobacillus plantarum, lactic acid bacteria, Lactobacillus casei, Lactobacillus acidophilus, Bifidobacterium bifidum and Lactococcus lactis in a mass ratio of 1:1:1:1:1:1, and the effective live bacteria count of Lactobacillus plantarum is ≥ 50 million cuf / g, the effective live bacteria count of lactic acid bacteria is ≥ 50 million cuf / g, the effective live bacteria count of Lactobacillus casei is ≥ 50 million cuf / g, the effective live bacteria count of Lactobacillus acidophilus is ≥ 50 million cuf / g, the effective live bacteria count of Bifidobacterium bifidum is ≥ 50 million cuf / g, and the effective live bacteria count of Lactococcus lactis is ≥ 50 million cuf / g; S4.5: centrifuge the fermented solution of the plant-derived composition for 10 minutes, take the supernatant, and perform vacuum low-temperature concentration and spray drying on the supernatant. The vacuum low-temperature concentration temperature is 50° C. to obtain the fermented powder of the plant-derived composition; S5: Preparation of plant-derived skin rejuvenation oral solution, 20 parts by weight of the inclusion composition, 24 parts by weight of fermented powder of the plant-based composition, 6 parts by weight of Pleurotus eryngii concentrated powder, 2 parts by weight of bird's nest peptide, 4 parts by weight of sodium hyaluronate, 2.5 parts by weight of Haematococcus pluvialis powder and 600 parts by weight of distilled water are stirred and mixed, and then filtered, filled and sterilized to obtain a plant-based skin rejuvenation oral liquid.

[0026] Comparative Example 1 Compared with Example 1, the difference of Comparative Example 1 is that step S3.4 is removed, and the emblica extract emulsion and the okra pectin extract-gelatin complex are directly stirred and mixed in a volume ratio of 3:8 to obtain a mixture of the emblica extract emulsion and the okra pectin extract-gelatin complex.

[0027] In step S5: in the preparation of the plant-derived skin rejuvenation oral liquid, the inclusion composition is replaced with a mixture of equal weight parts of emblica extract emulsion and okra pectin extract-gelatin complex, and the other steps and ingredients remain unchanged to prepare the plant-derived skin rejuvenation oral liquid, which is recorded as Comparative Example 1.

[0028] Comparative Example 2 Compared with Example 1, the difference of Comparative Example 2 is that in S5: Preparation of plant-derived skin rejuvenation oral liquid, the inclusion composition is replaced with an equal weight portion of emblica extract, and the other steps and ingredients remain unchanged to prepare the plant-derived skin rejuvenation oral liquid, which is recorded as Comparative Example 2.

[0029] Comparative Example 3 Compared with Example 1, the difference of Comparative Example 3 is that steps S4.4-S4.5 are removed, the enzymatic hydrolysis solution of the plant-based composition is stirred for reaction at 100°C for 10 minutes, and then directly centrifuged for 10 minutes, the supernatant is taken, and the supernatant is vacuum-low-temperature concentrated and spray-dried at a temperature of 50°C to obtain a plant-based composition powder.

[0030] Step S5: In the preparation of the plant-based skin rejuvenation oral liquid, the fermented plant-based composition powder is replaced with an equal weight portion of the plant-based composition powder, and the remaining steps and ingredients remain unchanged to prepare the plant-based skin rejuvenation oral liquid, which is recorded as Comparative Example 3.

[0031] Test Example 1 Sensory evaluation A sensory evaluation panel of 10 people was selected to conduct sensory evaluation on the plant-derived skin rejuvenation oral liquids of Examples 1-3 and Comparative Examples 1-2. Each assessor put 5 mL of the plant-derived skin rejuvenation oral liquid in his mouth each time for sensory evaluation, so that the plant-derived skin rejuvenation oral liquid fully filled the entire oral cavity, stayed for 10 seconds, and paid attention to whether there was a bitter aftertaste. The mouth was rinsed 3 times before and after each sensory evaluation. The total score of the sensory evaluation was 10 points. The sensory evaluation criteria were as follows:

[0032] According to the sensory evaluation standard, the plant-derived skin rejuvenation oral liquids of Examples 1-3 and Comparative Examples 1-2 were subjected to sensory evaluation, and the average sensory score of the assessors was finally taken as the result. The results are shown in Table 1 below.

[0033] Table 1:

[0034] As shown in Table 1, after sensory evaluation, the sensory scores of Examples 1-3 were all greater than 8 points, and both the aroma and taste were better than those of Comparative Examples 1-2, indicating that the inclusion treatment of the Emblica officinalis extract using the okra pectin extract-gelatin complex can effectively mask the bitter taste in the Emblica officinalis extract and improve the drinking taste of the plant-based skin rejuvenation oral liquid.

[0035] Test Example 2 Determination of active ingredient content The contents of rutin, quercetin and kaempferol in the plant-derived skin rejuvenation oral liquids of Examples 1-3 and the plant-derived skin rejuvenation oral liquid of Comparative Example 3 were detected by high performance liquid chromatography. The results are shown in Table 2 below.

[0036] Table 2:

[0037] As can be seen from Table 2, the contents of rutin, quercetin and kaempferol in the plant-based skin rejuvenation oral liquid of Comparative Example 3 are lower than those in the plant-based skin rejuvenation oral liquid of Examples 1-3, indicating that, compared with the plant-based composition powder that has not been fermented, the fermented powder of the plant-based composition has significantly higher effective contents of rutin, quercetin and kaempferol. Adding the fermented powder of the plant-based composition to the plant-based skin rejuvenation oral liquid is more conducive to increasing the effective content of the active ingredients rutin, quercetin and kaempferol.

[0038] Test Example 3 In vitro inhibition of anti-glycosylation end products The inclusion composition, okra pectin extract-gelatin complex, plant-based composition fermentation powder and emblica extract prepared in Example 1 were named Sample A1, Sample A2, Sample A3 and Sample A4, respectively; the mixture of emblica extract emulsion and okra pectin extract-gelatin complex in Comparative Example 1 was named Sample B1; and the plant-based composition powder in Comparative Example 3 was named Sample C1.

[0039] A phosphate buffer solution with a concentration of 0.2 mol / L and pH = 7.4 was used as the matrix liquid to prepare a solution containing a final concentration of 5% bovine serum albumin solution and 1 mol / L glucose (hereinafter referred to as: protein high-sugar solution). Subsequently, the protein high-sugar solution was used to prepare experimental samples of different concentrations: sample A1, sample A2, sample A3, sample A4, sample B1 and sample C1 were set up with three dose groups of low (0.5 g / L), medium (2 g / L) and high (4 g / L) as experimental groups. At the same time, a positive control group and a negative control group were prepared, in which aminoguanidine (4 g / L) was used as the positive control group, and a protein high-sugar solution without the test object was used as the negative control group. The above liquids were sterilized and filtered through a filter membrane, dispensed into 1.5 mL conical bottom centrifuge tubes, and incubated continuously for 28 days at a constant temperature of 37 ° C and aerobic light-proof conditions. Different experimental samples were taken once every 7 days, and samples were taken 10 times each time. All samples were taken out and stored in a refrigerator at -20 ° C. The fluorescence intensity of each experimental sample was measured using a Shimadzu RF-510 fluorescence spectrophotometer with an excitation wavelength of 370 nm and an emission wavelength of 440 nm.

[0040] The inhibition rates of the experimental group and the positive control group on AGEs were calculated, where inhibition rate (%) = (fluorescence intensity of the negative control group - fluorescence intensity of the experimental group or positive control group) / fluorescence intensity of the negative control group. The inhibition rate results are shown in Table 3.

[0041] Table 3:

[0042] As shown in Table 3, each sample in the experimental group has the function of inhibiting the formation of AGEs. Among them, the inclusion composition (sample A1) is weaker than the mixture of the emblica extract emulsion and the okra pectin extract-gelatin complex (sample B1) in the first 14 days. However, it can be seen that its inhibitory effect on AGEs is significantly better during the period from 21 to 28 days, indicating that the inhibitory effect of the inclusion composition on AGEs gradually increases with the passage of time, and it can more effectively reduce the formation of AGEs and slow down the formation process of AGEs. Compared with the emblica extract (sample A4), the inhibitory effect of the inclusion composition (sample A1) on AGEs is better than that of the emblica extract on AGEs, indicating that the emblica extract component and the okra pectin extract-gelatin complex component in the inclusion composition can synergize with each other to further promote the inhibitory effect of AGEs. Compared with the plant-based composition powder (sample C1), the plant-based composition fermented powder (sample A3) has a more significant inhibitory effect on AGEs, indicating that after fermentation treatment, the obtained plant-based composition fermented powder is more conducive to reducing the formation of AGEs.

[0043] Test Example 4 Shelf life test 1 g of each of the inclusion composition prepared in Example 1 and the mixture of the emblica extract emulsion and okra pectin extract-gelatin complex prepared in Comparative Example 1 was weighed as a sample. 0.5 g of each sample was taken and the gallic acid content therein was detected by high performance liquid chromatography; the remaining 0.5 g of sample was left to stand at 30°C ± 2°C for 60 days. After the standing period, the gallic acid content of the 0.5 g sample after standing was detected by high performance liquid chromatography. The gallic acid content of the sample before and after standing was recorded, and the loss rate of the gallic acid content before and after standing was calculated, wherein the loss rate (%) = (gallic acid content of the sample that was not standing - gallic acid content of the sample that was standing) / gallic acid content of the sample that was not standing. The results are as follows. Figure 2 shown.

[0044] like Figure 2It can be seen that compared with the mixture of the emblica extract emulsion and the okra pectin extract-gelatin complex prepared in Comparative Example 1, the content of gallic acid in the inclusion composition prepared in Example 1 changes less before and after standing, and its loss rate result is significantly smaller, indicating that the emblica extract is encapsulated by the okra pectin extract-gelatin complex, and the obtained inclusion composition can tightly wrap the emblica extract, effectively prevent the external environment from directly contacting and destroying the emblica extract, and better retain the active ingredients of the emblica extract.

[0045] It should be understood that those skilled in the art can make improvements or changes based on the above description, and all such improvements and changes should fall within the scope of protection of the appended claims of the present invention. Parts not described in detail in this specification belong to the prior art known to those skilled in the art.

Claims

1. A plant-derived skin rejuvenation oral liquid, characterized in that: Including the following raw materials: 5-20 parts by weight of the inclusion composition, 3-24 parts by weight of fermented plant composition powder, 1-6 parts by weight of Pleurotus eryngii concentrated powder, 0.3-2 parts by weight of bird's nest peptide, 1-4 parts by weight of sodium hyaluronate, 0.5-2.5 parts by weight of Haematococcus pluvialis powder and 300-600 parts by weight of distilled water; The raw materials of the inclusion composition include: an emblica extract emulsion and an okra pectin extract-gelatin complex, wherein the mass ratio of the emblica extract emulsion to the okra pectin extract-gelatin complex is (1-5): (3-10); The raw materials of the emblica extract emulsion include: an emblica extract solution and an oil, wherein the volume ratio of the emblica extract solution to the oil is (1-2):(2-4), the emblica extract solution is prepared by mixing the emblica extract with distilled water, and the concentration of the emblica extract solution is 20-30 mg / mL; The okra pectin extract-gelatin complex is prepared by stirring a 1-3% complex dispersion and adjusting the pH value to 2.2-3.8, wherein the 1-3% complex dispersion is prepared by mixing the okra pectin extract and gelatin in a mass ratio of (1-4): (1-3), and then mixing with distilled water; The raw materials of the plant-derived composition fermentation powder include: a plant-derived composition enzymatic solution and a composite fermentation bacterial agent, wherein the added amount of the composite fermentation bacterial agent is 1 to 5% of the plant-derived composition enzymatic solution; The plant-based composition enzymatic hydrolysis solution is obtained by subjecting the plant-based composition solution to enzymatic hydrolysis reactions of pectinase and cellulase. The plant-based composition solution is prepared by mixing the plant-based composition and distilled water in a mass ratio of 1:(26-38). The raw materials of the plant-based composition include: 8-18 parts by weight of mulberry leaves, 3-15 parts by weight of buckwheat, 2-9 parts by weight of locust flowers, 5-16 parts by weight of honeysuckle, 10-30 parts by weight of apples and 6-24 parts by weight of grapes.

2. The plant-derived skin rejuvenation oral liquid according to claim 1, characterized in that: The oil is prepared by mixing rice bran oil and polyglycerol ricinoleate, wherein the volume fraction of polyglycerol ricinoleate is 5%.

3. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 1, characterized in that: The steps include: S1: Okra separation and extraction treatment, drying and grinding okra into okra powder, preparing okra powder into okra solution, and subjecting the solution to microwave extraction, deproteinization, centrifugal separation, dialysis and freeze drying to obtain okra pectin extract; S2: Preparation of okra pectin extract-gelatin complex, mixing okra pectin extract and gelatin according to a proportion, adding distilled water to prepare a complex dispersion, heating and stirring for a period of time, and adjusting the pH of the complex dispersion to 2.2-3.8 to obtain the okra pectin extract-gelatin complex; S3: Preparation and inclusion processing of emblica extract, the emblica is crushed and mixed with an ethanol aqueous solution, and the emblica extract is obtained by reflux extraction, suction filtration, decompression removal of ethanol, freeze drying and grinding, the emblica extract is mixed with distilled water and oil to obtain an emblica extract emulsion, the emblica extract emulsion and okra pectin extract-gelatin complex are mixed, and the inclusion composition is obtained by high-speed homogenization and freeze drying; S4: fermentation treatment of the plant-derived composition, mulberry leaves, buckwheat, Sophora japonica, honeysuckle, apples and grapes are crushed and mixed to obtain the plant-derived composition, the plant-derived composition is mixed with distilled water, pectinase and cellulase are added to carry out enzymolysis reaction, and then a composite fermentation agent is added to carry out fermentation reaction. After the fermentation is completed, centrifugation is carried out, the supernatant is taken, vacuum concentration is carried out and spray drying is carried out to obtain fermentation powder of the plant-derived composition; S5: Preparation of plant-derived skin rejuvenation oral liquid: the inclusion composition, plant-derived composition fermentation powder, Pleurotus eryngii concentrated powder, bird's nest peptide, sodium hyaluronate, Haematococcus pluvialis powder and distilled water are stirred and mixed, and the plant-derived skin rejuvenation oral liquid is obtained through filtering, filling and sterilization.

4. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 3, characterized in that: Step S1: separation and extraction of okra, specifically comprising the following steps: S1.1: Wash the okra, cut it into pieces, freeze-dry it and grind it to obtain okra powder; S1.2: Okra powder and distilled water are mixed in a mass ratio of 1: (10-50) to obtain an okra solution, and the okra solution is subjected to microwave extraction for 6-15 minutes at a temperature of 40-60°C and a microwave power of 500-900W. Subsequently, the microwave-extracted solution is centrifuged at 6000-8000rpm for 6-12 minutes, and the supernatant is collected. The microwave extraction, centrifugation and supernatant are repeatedly performed on the remaining solution after the supernatant is collected. The above operation is repeated 2-4 times, and the supernatants obtained each time are combined to obtain an okra extract; S1.3: The okra extract is concentrated under reduced pressure to 1 / 4 to 1 / 3 of the original volume, and the concentrated okra extract is mixed with an organic solvent mixture according to a volume ratio of (2 to 4): 1, and stirred for 20 to 30 minutes. Subsequently, the mixed solution of the concentrated okra extract and the organic solvent mixture is centrifuged at 6000 to 8000 rpm for 5 to 10 minutes, the upper liquid is taken, the protein precipitate and the lower organic layer are discarded, and the above-mentioned organic solvent mixture mixing, stirring, centrifugal separation and upper liquid collection operations are repeated for the upper liquid until no protein appears, thereby obtaining a deproteinized extract; S1.4: Mix the deproteinized extract and anhydrous ethanol in a volume ratio of 1:(2-4), store at 3-5°C for 10-16 hours, and then centrifuge. Put the precipitate obtained after centrifugation into a dialysis bag and dialyze it for 24-48 hours. The molecular weight cutoff of the dialysis bag is 3500Da. Then, freeze-dry to obtain the okra pectin extract.

5. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 4, characterized in that: In step S1.3, the organic solvent mixture is prepared by mixing chloroform and n-butanol in a volume ratio of 4:

1.

6. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 5, characterized in that: Step S2: Preparation of okra pectin extract-gelatin complex, specifically comprising the following steps: S2.1: okra pectin extract and gelatin are mixed in a mass ratio of (1-4):(1-3), and then distilled water is added to prepare a composite dispersion with a mass fraction of 1-3%; S2.2: The complex dispersion is magnetically stirred at 40-50° C. for 2-3 h, and then a 1 mol / L hydrochloric acid solution is added to adjust the pH of the complex dispersion to 2.2-3.8, and the mixture is slowly stirred for 5-15 min to obtain an okra pectin extract-gelatin complex.

7. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 6, characterized in that: Step S3: Preparation and inclusion processing of Phyllanthus emblica extract, specifically comprising the following steps: S3.1: crush the emblica buds and pass through a 150-250 mesh sieve to obtain emblica buds coarse powder; S3.2: The emblica powder and 70-80% ethanol aqueous solution are mixed in a mass ratio of 1: (80-120), and the mixed solution is refluxed for 2-3 times at 70-75°C in a nitrogen atmosphere, and the time of each reflux extraction is 1-2 hours. The extracts obtained after each reflux extraction are combined, and the combined extracts are cooled to room temperature, filtered, decompressed to remove ethanol, freeze-dried and ground to obtain the emblica extract; S3.3: adding the emblica extract to distilled water to prepare an emblica extract solution with a concentration of 20-30 mg / mL, mixing the emblica extract solution with an oil liquid in a volume ratio of (1-2):(2-4), and subjecting the mixture of the emblica extract solution and the oil liquid to high-speed shearing using a high-speed shear emulsifier at a shear speed of 22000-28000 rpm and a shearing time of 3-5 min to obtain an emblica extract emulsion; S3.4: The emblica extract emulsion and the okra pectin extract-gelatin complex are mixed in a volume ratio of (1-5): (3-10), and the mixed solution of the emblica extract emulsion and the okra pectin extract-gelatin complex is homogenized at high speed using a homogenizer for 3-5 minutes, followed by freeze-drying to obtain an inclusion composition.

8. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 7, characterized in that: Step S4: fermentation treatment of the plant-derived composition, specifically comprising the following steps: S4.1: 8 to 18 parts by weight of mulberry leaves, 3 to 15 parts by weight of buckwheat, 2 to 9 parts by weight of Sophora japonica, 5 to 16 parts by weight of honeysuckle, 10 to 30 parts by weight of apples and 6 to 24 parts by weight of grapes are respectively crushed into powders and passed through a 200 to 300 mesh sieve, and all the powders obtained after sieving are mixed to obtain a plant-derived composition; S4.2: Mix the plant-derived composition and distilled water in a mass ratio of 1:(26-38) and then add sodium bicarbonate to adjust the pH of the mixed solution of the plant-derived composition and distilled water to 4.5-5.4 to obtain a plant-derived composition solution; S4.3: adding pectinase and cellulase to the plant-derived composition solution, and performing enzymolysis reaction at 30-50° C. for 2-4 hours. After the enzymolysis is completed, adding sodium bicarbonate, and adjusting the pH of the plant-derived composition solution after enzymolysis to 5.5-6.0, to obtain an enzymolysis solution of the plant-derived composition; S4.4: stirring the enzymatic hydrolysis solution of the plant-derived composition at 80-100° C. for 10-30 minutes, then cooling to 25-35° C., adding a composite fermentation agent to the enzymatic hydrolysis solution of the plant-derived composition, wherein the amount of the composite fermentation agent added is 1-5% of the enzymatic hydrolysis solution of the plant-derived composition, and fermenting for 2-5 days to obtain a fermentation solution of the plant-derived composition; S4.5: Centrifuge the fermented solution of the plant-derived composition for 5 to 10 minutes, take the supernatant, and perform vacuum low-temperature concentration and spray drying on the supernatant. The temperature of the vacuum low-temperature concentration is 45 to 60° C. to obtain the fermented powder of the plant-derived composition.

9. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 8, characterized in that: In step S4.3, the added amount of pectinase and cellulase is 2-6% of the plant-based composition solution, the enzyme activity of cellulase is 5000-20000 U / g, and the enzyme activity of pectinase is 5000-20000 U / g.

10. The method for preparing the plant-derived skin rejuvenation oral liquid according to claim 9, characterized in that: In step S4.4, the composite fermentation bacteria agent is composed of Lactobacillus plantarum, lactic acid bacteria, Lactobacillus casei, Lactobacillus acidophilus, Bifidobacterium bifidum and Lactococcus lactis in a mass ratio of 1:1:1:1:1:1:1, and the effective live bacteria count of Lactobacillus plantarum is ≥50 million cuf / g, the effective live bacteria count of lactic acid bacteria is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus casei is ≥50 million cuf / g, the effective live bacteria count of Lactobacillus acidophilus is ≥50 million cuf / g, the effective live bacteria count of Bifidobacterium bifidum is ≥50 million cuf / g, and the effective live bacteria count of Lactococcus lactis is ≥50 million cuf / g.

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