Qi-benefiting and blood-nourishing composition and application thereof

Abstract

The invention discloses a qi-benefiting and blood-nourishing composition and application thereof. The composition comprises the following raw materials: sea buckthorn fruit powder, emblic leafflower fruit powder, rhizoma polygonati powder, black date powder, chickpea powder and purple perilla seed powder. The preparation method of the fermented composition with the efficacy of benefiting qi and nourishing blood comprises the following steps: respectively sieving the sea buckthorn fruit powder, the emblic leafflower fruit powder, the rhizoma polygonati powder, the black date powder, the chickpea powder and the purple perilla seed powder, and uniformly mixing to obtain mixed powder; adding sterile deionized water into the mixed powder, and sterilizing to obtain a mixed solution; inoculating a mixed strain solution into the mixed solution, and stirring and fermenting; and after the fermentation is finished, filtering, and freeze-drying the filtrate to obtain the fermented composition with the effects of benefiting qi and nourishing blood. The plant extract is extracted through mixed fermentation of various microorganisms, natural active substances in the plant extract are enriched, the variety of the natural active substances is enriched, and the effect of enhancing immunity is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of food health care, and particularly relates to a qi-tonifying and blood-nourishing composition and its application. Background Art

[0002] In traditional Chinese medicine theory, qi-tonifying and blood-nourishing formulas mostly use plant raw materials that are both food and medicine, and are prepared by decocting or simple extraction. However, such methods have problems such as low release rate of active ingredients, easy loss of heat-sensitive substances, and insufficient bioavailability. In recent years, microbial fermentation technology has been introduced into the field of plant active ingredient extraction. Through enzymatic hydrolysis, it can effectively break the cell structure of plants and release functional ingredients such as polysaccharides and flavonoids. However, existing fermentation processes mostly use a single strain (such as lactic acid bacteria or yeast), which has problems such as single metabolic products, low synergistic conversion efficiency, and insufficient regulation of trace elements and prebiotics during the fermentation process, restricting the enrichment and functional enhancement of active ingredients. In addition, existing compositions mostly focus on the extraction of single functional ingredients, ignoring the synergistic effect generated by the fermentation of various plant matrices, resulting in limitations in comprehensive effects such as improving hematopoietic function and enhancing immunity. Summary of the Invention

[0003] To solve the above technical problems, the purpose of the present invention is to provide a fermented composition with qi-tonifying and blood-nourishing effects prepared based on microbial fermentation technology and its application.

[0004] To achieve the above purpose, the present invention provides the following technical solutions:

[0005] In the first aspect, the present invention provides a fermented composition with qi-tonifying and blood-nourishing effects, including the following raw materials;

[0006] Seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, perilla seed powder.

[0007] In the second aspect, the present invention provides a preparation method of the fermented composition with qi-tonifying and blood-nourishing effects described in the first aspect, including the following steps:

[0008] T1: After passing the seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder through an 80-100 mesh sieve respectively, mix them according to the formula amount to obtain a mixed powder, prepare the mixed powder into a 10-15 wt% mixed solution with deionized water, and perform ultra-high temperature instantaneous sterilization on the mixed solution to obtain a sterile mixed solution;

[0009] T2: Inoculate the sterile mixture with 10 - 15 v / v% of the mixed bacterial solution, ferment at 32 ± 3°C for 48 - 72 h, and filter after fermentation to obtain the fermentation filtrate. Among them, the mixed bacterial solution contains Lactobacillus plantarum with the preservation number of CCTCC NO. M2015128 and Saccharomyces boulardii with the preservation number of CCTCC NO. M2014211. The total viable count of the mixed bacterial solution is 1×10 8 -1×10 10 CFU / mL, and the viable count ratio of Lactobacillus plantarum to Saccharomyces boulardii is 1:0.5 - 2;

[0010] T3: Lyophilize the fermentation filtrate to obtain the fermentation composition.

[0011] Preferably, the mass ratio of seabuckthorn fruit powder, phyllanthus emblica powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder in the mixed powder is 1:0.8 - 2:1.5 - 3:0.5 - 1.5:1 - 2:0.3 - 1.

[0012] Preferably, the fermentation is carried out by first performing anaerobic fermentation for 24 - 36 h and then aerobic fermentation for 24 - 36 h.

[0013] Preferably, the oxygen supply amount for aerobic fermentation is 0.1 - 0.3 vvm.

[0014] Preferably, during aerobic fermentation, sodium selenite accounting for 0.1 - 0.5 wt% of the mass of the mixed powder and chicory inulin accounting for 3 - 5 wt% of the mass of the mixed powder are supplemented and added.

[0015] In the third aspect, the present invention provides an application of the fermentation composition with the efficacy of supplementing qi and nourishing blood prepared by the first aspect or the second aspect in the preparation of health products for supplementing qi and blood.

[0016] In the fourth aspect, the present invention provides a health product for supplementing qi and blood, and the health product for supplementing qi and blood contains the fermentation composition with the efficacy of supplementing qi and blood prepared by the first aspect or the second aspect.

[0017] Preferably, the health product for supplementing qi and blood further includes a filler, a binder, and a disintegrant.

[0018] Preferably, the filler is starch;

[0019] Preferably, the binder is hydroxypropyl methylcellulose and / or polyvinylpyrrolidone K30;

[0020] Preferably, the disintegrant is sodium carboxymethyl starch and / or cross-linked carboxymethyl cellulose sodium.

[0021] Compared with the prior art, the beneficial effects of the present invention are:

[0022] Through the specific ratio (1:0.5 - 2 viable bacteria ratio) of Lactobacillus plantarum CCTCC NO.M2015128 and Saccharomyces boulardii CCTCC NO.M2014211, the complementary advantages of two-stage metabolism are realized. In the anaerobic stage, Lactobacillus plantarum secretes cellulase to efficiently break the cell wall and release iron elements and flavonoid aglycones. In the aerobic stage, Saccharomyces boulardii converts precursor substances into methylated active ingredients. With the prebiotic effect of chicory inulin, the production of short-chain fatty acids such as butyric acid is increased, significantly promoting intestinal iron absorption and the secretion of erythropoietin.

[0023] Adopt an anaerobic (24 - 36h)-aerobic (24 - 36h) staged fermentation strategy. In the aerobic stage, sodium selenite (0.1 - 0.5 wt%) is added synchronously to form a selenium-iron complex transporter, effectively improving the efficiency of hemoglobin synthesis.

[0024] Seabuckthorn fruit powder - Phyllanthus emblica fruit powder - Polygonatum sibiricum powder can form an antioxidant-hematopoietic synergistic chain. The high content of vitamin C in seabuckthorn fruit powder, as a reducing agent, can convert gallic acid in Phyllanthus emblica fruit powder into more active free phenolic acids, while protecting Polygonatum sibiricum polysaccharide from oxidative degradation. The β-glucosidase produced by Lactobacillus plantarum fermentation hydrolyzes ellagitannin in Phyllanthus emblica fruit powder into ellagic acid, forming a π-π conjugate structure with seabuckthorn flavonoids, further enhancing its antioxidant capacity. Polygonatum sibiricum polysaccharide is oxidized and cleaved by Saccharomyces boulardii into oligosaccharides, which together with seabuckthorn VC activate the TLR4 / NF-κB pathway of bone marrow stromal cells, promoting erythropoiesis.

[0025] Black date powder - chickpea powder can form an iron-selenium-protein ternary carrier system. Iron elements in black date powder are chelated by acidic substances secreted by Lactobacillus plantarum during the anaerobic fermentation stage and converted into soluble Fe ions; the peptide segments produced by chickpea powder fermentation bind to Fe ions through histidine residues to form an iron-peptide complex, improving iron absorption rate; the selenium in sodium selenite added in the aerobic stage is co-loaded with the iron-peptide complex, synchronously promoting the iron absorption rate to enhance the blood-tonifying effect.

[0026] Perilla seed powder - chicory inulin can form a membrane stability-prebiotic synergy. The ω-3 fatty acids in perilla seed powder are oxidized through fermentation to generate anti-inflammatory mediators and reduce the intestinal IL-6 level induced by cyclophosphamide; chicory inulin, as a prebiotic, promotes the proliferation of microorganisms; the acidic substances produced by microbial metabolism synergistically reduce iron loss with perilla α-linolenic acid derivatives, enhancing the blood-tonifying effect. Specific implementation method

[0028] To better understand the present invention, the present invention will be further described below in conjunction with specific embodiments. The terms used in the embodiments are for describing specific specific implementation schemes and do not constitute a limitation on the protection scope of the present invention.

[0029] For the experimental methods without specific conditions noted in the following examples, they are generally carried out under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0030] The sources of some raw materials are as follows:

[0031] Seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder were purchased from Waterex Biological.

[0032] Lactobacillus plantarum was purchased from the China Center for Type Culture Collection, strain number: CCTCC M2015128;

[0033] Commercially available Lactobacillus plantarum: purchased from Xi'an Meihe Biotechnology Co., Ltd., product number: MH-2024051603;

[0034] Saccharomyces boulardii was purchased from the China Center for Type Culture Collection, strain number: CCTCC M2014211;

[0035] Commercially available Saccharomyces cerevisiae boulardii: purchased from Xi'an Meihe Biotechnology Co., Ltd., product number MH-22122706;

[0036] Inulin from Cichorium intybus: purchased from Altay (Guangzhou) Biotechnology Co., Ltd., product number: CNE60308;

[0037] Other raw materials and reagents were all purchased commercially.

[0038] Preparation of a fermentation composition with the efficacy of supplementing qi and nourishing blood

[0039] Composition 1:

[0040] T1: After passing seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder through a 100-mesh sieve respectively, they were mixed according to the formula amount to obtain a mixed powder. The mixed powder was formulated into a 13 wt% mixed solution with deionized water, and the mixed solution was subjected to ultra-high temperature instantaneous sterilization to obtain a sterile mixed solution;

[0041] T2: 13 v / v% of the mixed bacterial solution was inoculated into the sterile mixed solution, and fermentation was carried out at 32 °C for 60 h. After fermentation, filtration was carried out to obtain a fermentation filtrate. Among them, the mixed bacterial solution contained Lactobacillus plantarum with the preservation number CCTCC NO. M2015128 and Saccharomyces boulardii with the preservation number CCTCC NO. M2014211. The total viable count of the mixed bacterial solution was 1×109 CFU / mL, the viable cell count ratio of the Lactobacillus plantarum to the Saccharomyces boulardii is 1:1.5;

[0042] T3: Lyophilize the fermentation filtrate to obtain a fermentation composition.

[0043] In the mixed powder, the mass ratio of seabuckthorn fruit powder, phyllanthus emblica powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder is 1:1.4:2.2:1:1.5:0.7.

[0044] The fermentation is carried out by first performing anaerobic fermentation for 30 h and then aerobic fermentation for 30 h.

[0045] The oxygen flow rate for the aerobic fermentation is 0.2 vvm.

[0046] During aerobic fermentation, sodium selenite accounting for 0.3 wt% of the mass of the mixed powder and chicory inulin accounting for 4 wt% of the mass of the mixed powder are supplemented and added.

[0047] Composition 2:

[0048] T1: After passing seabuckthorn fruit powder, phyllanthus emblica powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder through an 80-mesh sieve respectively, mix them according to the formula amount to obtain a mixed powder. Prepare the mixed powder into a 15 wt% mixed solution with deionized water, and perform ultra-high temperature instantaneous sterilization on the mixed solution to obtain a sterile mixed solution;

[0049] T2: Inoculate 10 v / v% of the mixed bacterial solution into the sterile mixed solution, ferment at 29 °C for 72 h, and filter after the fermentation ends to obtain a fermentation filtrate. Among them, the mixed bacterial solution contains Lactobacillus plantarum with the preservation number of CCTCC NO. M2015128 and Saccharomyces boulardii with the preservation number of CCTCC NO. M2014211. The total viable cell count of the mixed bacterial solution is 1×10 8 CFU / mL, the viable cell count ratio of the Lactobacillus plantarum to the Saccharomyces boulardii is 1:0.5;

[0050] T3: Lyophilize the fermentation filtrate to obtain a fermentation composition.

[0051] In the mixed powder, the mass ratio of seabuckthorn fruit powder, phyllanthus emblica powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder is 1:0.8:3:1.5:1:0.3.

[0052] The fermentation is carried out by first performing anaerobic fermentation for 36 h and then aerobic fermentation for 36 h.

[0053] The oxygen flow rate for the aerobic fermentation is 0.1 vvm.

[0054] When performing aerobic fermentation, sodium selenite accounting for 0.1 wt% of the mass of the mixed powder and chicory inulin accounting for 5 wt% of the mass of the mixed powder are supplemented and added.

[0055] Composition 3:

[0056] T1: The seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder are respectively passed through a 90-mesh sieve, then mixed according to the formula amount to obtain a mixed powder. The mixed powder is formulated into a 10 wt% mixed solution with deionized water, and the mixed solution is subjected to ultra-high temperature instantaneous sterilization to obtain a sterile mixed solution;

[0057] T2: Inoculate 15 v / v% of the mixed bacterial solution into the sterile mixed solution, ferment at 35 °C for 48 h, and filter after fermentation to obtain a fermentation filtrate. Among them, the mixed bacterial solution contains Lactobacillus plantarum with the preservation number CCTCC NO. M2015128 and Saccharomyces boulardii with the preservation number CCTCC NO. M2014211. The total viable count of the mixed bacterial solution is 1×10 10 CFU / mL, and the viable count ratio of Lactobacillus plantarum to Saccharomyces boulardii is 1:2;

[0058] T3: Lyophilize the fermentation filtrate to obtain a fermentation composition.

[0059] In the mixed powder, the mass ratio of seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder is 1:2:1.5:0.5:2:1.

[0060] The fermentation is carried out by first performing anaerobic fermentation for 24 h and then aerobic fermentation for 24 h.

[0061] The oxygen supply amount for the aerobic fermentation is 0.3 vvm.

[0062] When performing aerobic fermentation, sodium selenite accounting for 0.5 wt% of the mass of the mixed powder and chicory inulin accounting for 3 wt% of the mass of the mixed powder are supplemented and added.

[0063] Composition ①: Different from Composition 1, Saccharomyces boulardii CCTCC NO. M2014211 is used to replace Lactobacillus plantarum CCTCC NO. M2015128, and the remaining steps and parameters are the same as those of Composition 1.

[0064] Composition ②: Different from Composition 1, Lactobacillus plantarum CCTCC NO. M2015128 is used to replace Saccharomyces boulardii CCTCC NO. M2014211, and the remaining steps and parameters are the same as those of Composition 1.

[0065] Composition ③: Different from Composition 1, commercially available Saccharomyces boulardii is used to replace Saccharomyces boulardii CCTCCNO.M2014211, and the remaining steps and parameters are the same as those of Composition 1.

[0066] Composition ④: Different from Composition 1, commercially available Lactobacillus plantarum is used to replace Lactobacillus plantarum CCTCC NO.M2015128, and the remaining steps and parameters are the same as those of Composition 1.

[0067] Composition ⑤: Different from Composition 1, the fermentation is anaerobic fermentation for 60 h. After 30 h of anaerobic fermentation, sodium selenite accounting for 0.3 wt% of the mass of the mixed powder and inulin from Cichorium intybus accounting for 8 wt% of the mass of the mixed powder are added. The remaining steps and parameters are the same as those of Composition 1.

[0068] Composition ⑥: Different from Composition 1, the fermentation is aerobic fermentation for 60 h. After 30 h of aerobic fermentation, sodium selenite accounting for 0.3 wt% of the mass of the mixed powder and inulin from Cichorium intybus accounting for 8 wt% of the mass of the mixed powder are added. For the aerobic fermentation, the oxygen flow rate is 0.2 vvm. The remaining steps and parameters are the same as those of Composition 1.

[0069] Composition ⑦: Different from Composition 1, sodium selenite is not added, and the remaining steps and parameters are the same as those of Composition 1.

[0070] Composition ⑧: Different from Composition 1, seabuckthorn fruit powder is missing, and the remaining ganoderma lucidum powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder are supplemented in a mass ratio of 1.4:2.2:1:1.5:0.7 based on the raw material quality. The remaining steps and parameters are the same as those of Composition 1.

[0071] Composition ⑨: Different from Composition 1, ganoderma lucidum powder is missing, and the remaining seabuckthorn fruit powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder are supplemented in a mass ratio of 1:2.2:1:1.5:0.7 based on the raw material quality. The remaining steps and parameters are the same as those of Composition 1.

[0072] Composition ⑩: Different from Composition 1, perilla seed powder is missing, and the remaining seabuckthorn fruit powder, ganoderma lucidum powder, polygonatum sibiricum powder, black date powder, and chickpea powder are supplemented in a mass ratio of 1:1.4:2.2:1:1.5 based on the raw material quality. The remaining steps and parameters are the same as those of Composition 1.

[0073] Composition Different from Composition 1, chickpea powder is missing, and the remaining seabuckthorn fruit powder, ganoderma lucidum powder, polygonatum sibiricum powder, black date powder, and perilla seed powder are supplemented in a mass ratio of 1:1.4:2.2:1:0.7 based on the raw material quality. The remaining steps and parameters are the same as those of Composition 1.

[0074] Verification test for the efficacy of supplementing qi and blood:

[0075] Select healthy clean - grade ICR mice, female, with a body weight of 18 - 22 g, 200 mice. One week before the experiment, the mice were adaptively fed, given sterilized mouse food and sterilized water, and allowed to feed and drink freely. Randomly select 160 mice and divide them into 16 groups, with 10 mice in each group;

[0076] Test samples: Composition 1 - 3,

[0077] Configuration of test samples: Dilute the test samples with drinking water to a 30 wt% mixture, and prepare it immediately before use.

[0078] Normal control group: Fed normal mouse food and water. From day 1 to day 14, each mouse was gavaged with drinking water at 0.2 g / Kg (body weight) per day. From day 15 to day 18, saline was intraperitoneally injected at 40 mg / kg per day for four consecutive days.

[0079] Model control group: Fed normal mouse food and water. From day 1 to day 14, each mouse was gavaged with drinking water at 0.2 g / Kg (body weight) per day. From day 15 to day 18, cyclophosphamide was intraperitoneally injected at 40 mg / kg per day for four consecutive days to prepare a blood - deficiency mouse model.

[0080] Test group 1: Ensure normal feeding of mouse food and water. From day 1 to day 14, the fermented composition with the effect of supplementing qi and nourishing blood prepared from Composition 1 was gavaged at 0.2 g / Kg (body weight) per day. From day 15 to day 18, cyclophosphamide was intraperitoneally injected at 40 mg / kg per day for four consecutive days.

[0081] Test group 2: Ensure normal feeding of mouse food and water. From day 1 to day 14, the fermented composition with the effect of supplementing qi and nourishing blood prepared from Composition 2 was gavaged at 0.2 g / Kg (body weight) per day. From day 15 to day 18, cyclophosphamide was intraperitoneally injected at 40 mg / kg per day for four consecutive days.

[0082] Test group 3: Ensure normal feeding of mouse food and water. From day 1 to day 14, the fermented composition with the effect of supplementing qi and nourishing blood prepared from Composition 3 was gavaged at 0.2 g / Kg (body weight) per day. From day 15 to day 18, cyclophosphamide was intraperitoneally injected at 40 mg / kg per day for four consecutive days.

[0083] Test group ①: Ensure normal feeding of mouse food and water. From day 1 to day 14, the fermented composition with the effect of supplementing qi and nourishing blood prepared from Composition ① was gavaged at 0.2 g / Kg (body weight) per day. From day 15 to day 18, cyclophosphamide was intraperitoneally injected at 40 mg / kg per day for four consecutive days.

[0084] Test group ②: Ensure normal feeding of mouse food and water. From day 1 to day 14, the fermented composition with the effect of supplementing qi and nourishing blood prepared from Composition ② was gavaged at 0.2 g / Kg (body weight) per day. From day 15 to day 18, cyclophosphamide was intraperitoneally injected at 40 mg / kg per day for four consecutive days.

[0085] Group ③ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ③ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0086] Group ④ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ④ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0087] Group ⑤ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ⑤ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0088] Group ⑥ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ⑥ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0089] Group ⑦ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ⑦ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0090] Group ⑧ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ⑧ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0091] Group ⑨ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ⑨ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0092] Group ⑩ of the experiment: Ensure normal feeding of rat food and water. From day 1 to day 14, intragastric administration of the fermented composition with the effect of invigorating qi and nourishing blood prepared from Composition ⑩ was carried out daily at a dose of 0.2 g / Kg (body weight). From day 15 to day 18, cyclophosphamide was intraperitoneally injected daily at a dose of 40 mg / kg for four consecutive days.

[0093] Experiment Group: Ensure normal feeding of mouse food and water. From day 1 to day 14, the composition was intragastrically administered at 0.2 g / Kg (body weight) daily. The prepared fermented composition with the efficacy of invigorating qi and nourishing blood was intraperitoneally injected with cyclophosphamide at 40 mg / kg daily for four consecutive days from day 15 to day 18.

[0094] Experimental results:

[0095] The measurement results of the number of red blood cells (RBC), white blood cells (WBC), and hemoglobin (HGB) in the blood of mice in each group are shown in Table 1.

[0096] Statistical software was used to process the data, and the results were expressed as mean ± standard deviation.

[0097] Table 1 Result statistics

[0098]

[0099]

[0100] Note: "*" indicates p < 0.05 compared with the model control group, and "#" indicates p < 0.05 compared with the normal control group.

[0101] According to the results in Table 1, it can be seen that in experimental groups 1 - 3 of the present invention compared with the model control group, the number of red blood cells, white blood cells, and hemoglobin in mice was significantly increased, with significant differences, verifying that the composition prepared by the present invention has a good efficacy of supplementing qi and blood.

[0102] Comparing the results of experimental group 1 with those of experimental groups ① - ④, it can be seen that the mixed fermentation of Saccharomyces boulardii CCTCC NO.M2014211 and Lactobacillus plantarum CCTCC NO.M2015128 of the present invention has better effects. This may be because during the mixed fermentation process, Lactobacillus plantarum secretes extracellular enzymes such as cellulase and pectinase to destroy the cell wall structure of plants such as sea buckthorn and polygonatum, releasing active ingredients such as flavonoids, saponins, and polysaccharides. Among them, flavonoid components may be converted into flavonoid compounds with higher activity after fermentation by Lactobacillus plantarum; after further redox by Saccharomyces boulardii, methylation derivatives are generated, further enhancing its antioxidant and hematopoiesis-promoting functions. In addition, polysaccharide components are cleaved into oligosaccharides under the combined action of Lactobacillus plantarum and Saccharomyces boulardii, further enhancing their utilization rate.

[0103] From the results of Comparative Experiment Group 5 - Experiment Group 6 and Experiment Group 1, it can be seen that the fermentation composition prepared by the specific fermentation method adopted in the present invention has a significant effect of supplementing qi and blood. This may be because in the anaerobic stage, the fiber degradation function of Lactobacillus plantarum is preferentially activated to release iron elements in plants. In the aerobic stage, butyric acid secreted by Saccharomyces boulardii is induced by the dissolved oxygen content, and inulin chicory is added synchronously to promote the metabolism of the flora. The butyric acid secreted by Saccharomyces boulardii and the propionic acid secreted by Lactobacillus plantarum help to stimulate intestinal epithelial cells to secrete erythropoietin and promote bone marrow hematopoiesis function.

[0104] From the results of Comparative Experiment Group 1 and Experiment Group 7, it can be seen that sodium selenite has a certain effect in the fermentation process presented in the present invention. The principle may be that the selenium ions in sodium selenite form a selenium - iron co - transport complex with the iron ions released by Lactobacillus plantarum, promoting the absorption of iron ions.

[0105] Comparative Experiment Group 1 and Experiment Group 8 - Experiment From the results of Group 8, it can be seen that when any one of seabuckthorn fruit powder, phyllanthus emblica powder, chickpea powder, and perilla seed powder is missing in the mixed solution, the qi - and - blood - supplementing effect of the obtained composition becomes worse. It can be seen that there is a significant synergistic effect among seabuckthorn fruit powder, phyllanthus emblica powder, polygonatum sibiricum powder, black date powder, chickpea powder, and perilla seed powder in the present invention.

[0106] Raw materials for a health product for supplementing qi and blood and its preparation method:

[0107] Health product 1: Composition 1: 30 wt%, Povidone K30: 1 wt%, Sodium carboxymethyl starch: 3 wt%, Corn starch to make up 100 wt%;

[0108] Health product 2: Composition 2: 30 wt%, Povidone K30: 1 wt%, Sodium carboxymethyl starch: 3 wt%, Corn starch to make up 100 wt%;

[0109] Health product 3: Composition 3: 30 wt%, Povidone K30: 1 wt%, Sodium carboxymethyl starch: 3 wt%, Corn starch to make up 100 wt%;

[0110] The preparation methods of Health products 1 - 3 include the following steps:

[0111] S1: Dissolve povidone in 95% ethanol to prepare an adhesive solution with a weight percentage content of 20%;

[0112] S2: Mix the composition, corn starch, hydroxypropyl methylcellulose evenly in a high - shear granulator to obtain a pre - mixture;

[0113] S3: Add a binder solution to the pre-mixture for wet granulation. The stirring speed is 180 rpm, the cutter speed is 1200 rpm, and the granulation time is 10 min to obtain soft materials. Dry the soft materials at 50 °C for 2 h, and then perform dry screening through a 20-mesh sieve to obtain granules. Mix the sodium carboxymethyl starch evenly with the granules to obtain the total mixed powder, and use a tablet press to press the total mixed powder into tablets.

[0114] Toxicity test

[0115] Select healthy clean-grade ICR mice, female, with a body weight of 18 - 22 g. Divide them into a control group and experimental groups 1 - 3, with 5 mice in each group. Among them, in experimental groups 1 - 3, administer 2 g / Kg of health products 1 - 3 by gavage every day, and administer an equal amount of normal saline by gavage to the control group every day. Measure the mortality results 24 h after administration.

[0116] The results show that all the mice in the control group and the experimental groups survived and were in good condition.

[0117] The embodiments described above are some, but not all, of the embodiments of the present application. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application claimed, but merely represents the selected embodiments of the present application. All other embodiments obtained by those skilled in the art based on the embodiments in the present application without creative efforts fall within the scope of protection of the present application.

Claims

1. A fermented composition with the efficacy of supplementing qi and nourishing blood, characterized in that, It includes the following raw materials: Seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, perilla seed powder, and the mass ratio of the seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, perilla seed powder is 1:0.8 - 2:1.5 - 3:0.5 - 1.5:1 - 2:0.3 - 1; The preparation method of the fermentation composition includes the following steps: T1: After passing the seabuckthorn fruit powder, Phyllanthus emblica powder, Polygonatum sibiricum powder, black date powder, chickpea powder, perilla seed powder through a 80 - 100 mesh sieve respectively, mix them to obtain a mixed powder, and prepare the mixed powder into a 10 - 15wt% mixed solution with deionized water, and perform ultra-high temperature instantaneous sterilization on the mixed solution to obtain a sterile mixed solution; T2: Inoculate 10 - 15 v / v% of the mixed bacterial solution into the sterile mixed solution, ferment at 32 ± 3 °C for 48 - 72 h, and filter after the fermentation ends to obtain the fermentation filtrate. Among them, the mixed bacterial solution contains Lactobacillus plantarum with the preservation number of CCTCC NO.M2015128 and Saccharomyces boulardii with the preservation number of CCTCC NO.M2014211. The total viable count of the mixed bacterial solution is 1×10 8 -1×10 10 CFU / mL, and the viable count ratio of Lactobacillus plantarum to Saccharomyces boulardii is 1:0.5 - 2; T3: Freeze-dry the fermentation filtrate to obtain the fermentation composition.

2. The fermented composition with the efficacy of supplementing qi and nourishing blood according to claim 1, wherein The fermentation is carried out by first performing anaerobic fermentation for 24 - 36h, and then performing aerobic fermentation for 24 - 36h.

3. The fermented composition with the efficacy of supplementing qi and nourishing blood according to claim 2, wherein The oxygen flow rate for the aerobic fermentation is 0.1 - 0.3vvm.

4. The fermented composition with the efficacy of supplementing qi and nourishing blood according to claim 2, wherein During aerobic fermentation, sodium selenite accounting for 0.1 - 0.5wt% of the mass of the mixed powder and inulin from Cichorium intybus accounting for 3 - 5wt% of the mass of the mixed powder are supplemented and added.

5. Use of the fermentation composition according to any one of claims 1 - 4 in the preparation of food or health products.

6. A health product for supplementing qi and nourishing blood, characterized in that, The health product contains the fermentation composition according to any one of claims 1 - 4.

7. The health product according to claim 6, wherein The dosage form of the health product is a granule, and the health product also includes a filler, a binder, and a disintegrant.

8. The health care product according to claim 7, wherein The filler is starch; the binder is hydroxypropyl methylcellulose and / or polyvinylpyrrolidone K30; the disintegrant is sodium carboxymethyl starch and / or cross-linked carboxymethyl cellulose sodium.

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