Kit used for staphylococcus aureus detection

Abstract

The invention provides a kit used for staphylococcus aureus detection. The kit used for staphylococcus aureus detection contains guide RNA of a specific targeted staphylococcus aureus rpoB gene, an amplification primer pair, hydrated TwistAmp basic kit reaction drying balls, LbCas12a protein, a single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor and buffer liquid. By using the kit for staphylococcus aureus detection, the detection specificity is high, and staphylococcus aureus and other staphylococcus, including staphylococcus epidermidis, staphylococcus hominis, staphylococcus warneri,staphylococcus capitis and staphylococcus haemolyticus, can be separated. Meanwhile, by using a RNA sequence for detection, elapsed time is about 1 hour, no PCR instruments are used, the equipment requirements are low, and are significantly lower than that of a traditional bacterial culture method or PCR sequencing method, and the clinical application valve is large.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for detection of Staphylococcus aureus, comprising a guide RNA specifically targeting the rpoB gene of Staphylococcus aureus, a pair of RPA amplification primers, and a hydrated TwistAmpbasic kit Reaction dry balls, LbCas12a protein, single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor, and buffer. This kit can be used for rapid detection of Staphylococcus aureus. Background technique [0002] Staphylococcus aureus is a common bacterial group among Gram-positive bacteria that infects human beings, which brings a huge burden to patients and society. The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus aureus from other staphylococci due to the lack of specific primers, so joint generation sequencing is required It can only be identified, the ...

AI Technical Summary

Problems solved by technology

The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus aureus from other staphylococci due to the lack of specific primers, so joint generation sequencing is required Can only be identified, the cost is high, and it takes several days to get the result

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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