32 results about "Erysipelothrix rhusiopathiae" patented technology

It is intended to provide a mutant of a surface protective antigen SpaA protein of Erysipelothrix rhusiopathiae or a shortened SpaA protein (DeltaSpaA) obtained by removing a part therefrom and a process for producing the same. By introducing an amino acid substituent into a definite position of the amino acid sequence of the SpaA protein or the DeltaSpaA protein, a mutant of the SpaA protein or the DeltaSpaA protein having immunogenicity and being expressed as an inclusion body in Escherichia coli can be obtained. Because of being expressed as an insoluble inclusion body in Escherichia coli, the above-described mutant of the SpaA protein or the DeltaSpaA protein can be easily collected and purified.

The invention discloses a pair of specific primers for detecting Bergeyella, a kit and a PCR detection method. The primers comprise an upstream primer Bergeyella-F: 5'-TTGAAAGCTCCGGCGGATAG-3' and a downstream primer Bergeyella-R: 5'-ACCCTCACGAGAGTAGGTTT-3'. According to the specific primers, the kit and the PCR detection method, streptococcus, bacillus erysipelatos-suis, pasteurella multocida, salmonella, haemophilus parasuis, infectious actinobacillus pleuropneumoniae, stenotrophomonas maltophilia, pig Bergeyella and a Bergeyella zoohelcum 16S rRNA gene sequence are downloaded from a GenBankdatabase; sequence comparison is carried out through MEGA 5.05 software to search a Bergeyella 16S rRNA specific sequence; and Primer-BLAST design is utilized to amplify a pair of specific primers ofBergeyella; and a Bergeyella PCR rapid detection method is established to identify Bergeyella. The method has the advantages of being rapid, convenient, high in sensitivity and strong in specificity.

The invention relates to the technical field of aquaculture, and discloses a feed additive for preventing and treating swine erysipelas. The feed additive is prepared from, codonopsis pilosula, rhizoma polygonati, polygonatum odoratum, sanguisorba officinalis, agrimonia pilosa, nabalus, geum, erose mussaenda leaf, cudrania cochinchinensis, thunbergia grandiflora, glabrous sarcandra herb, marsdenia tenacissima, eggshell powder, mirabilitum praeparatum, black soybean powder, dietary alkali, aspergillus oryzae, bacillus natto, lycopene and compound enzyme. The feed additive does not have any toxic reagents and antibiotics, cannot generate residue and is remarkable in preventing and treating efficiency, the occurrence rate of the swine erysipelas is reduced to 0-1%, enzymolysis of various Chinese herbal medicines is achieved, tissue cell structures are destroyed, the Chinese herbal medicines are doubly fermented, palatability is improved, effective components are effectively extracted, the extracting rate reaches 86-88%, the use ratio of the Chinese herbal medicines is increased, the dietary alkali and the lycopene are added, pH(potential of hydrogen) and disease-resistant components of the feed additive are improved, activities of swine erysipelas bacilli are restrained, resistance of pigs is improved, and the swine erysipelas and other diseases are fundamentally restrained, so that aquaculture cost is reduced by 13.6%.

The invention discloses a gene gapdh for expressing erysipelothrix rhusiopathiae recombinant protein GAPDH (glyceraldehyde-3-phosphate dehydrogenase) as well as recombinant escherichia coli and applications of the gene gapdh. According to the technical scheme, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of erysipelothrix rhusiopathiae is cloned, expressed and purified. The western blot analysis for the porcine erysipelas rehabilitation pig serum shows that the recombinant protein GAPDH has very good immunogenicity. The mouse experiment shows that the recombinant protein GAPDH caninduce generation of high-titer antibodies and generate effective protection force. A novel candidate antigen is provided for the creation of a novel porcine erysipelas vaccine.

A variant of Erysipelothrix rhusiopathiae surface protective antigen SpaA protein or of a shortened form of SpaA (ΔSpaA) in which a portion of SpaA protein is deleted for protection from Erysipelothrix rhusiopathiae infection and a process for preparing the same are provided. Introduction of amino acid substitution at a specific site in the amino acid sequence of SpaA or ΔSpaA protein provides a variant of SpaA or ΔSpaA protein which is immunogenic and is expressed in E. coli as inclusion bodies. The variant of SpaA or ΔSpaA protein of the present invention may easily be recovered and purified since it is expressed in E. coli as inclusion bodies.

The present invention provides a composition and an improved single dose vaccine against E. rhusiopathiae and an improved single dose vaccine against E. rhusiopathiae and H. parasuis which provides one or more of the following: 1) confers effective immunity against E. rhusiopathiae and / or H. parasuis; 2) decreases the risk of developing clinical signs of E. rhusiopathiae and / or H. parasuis infection; 3) induces an immune response against E. rhusiopathiae and / or H. parasuis; and 4) has a DOI against E. rhusiopathiae and / or H. parasuis of at least four months. The composition or E. rhusiopathiae vaccine as well as the combined E. rhusiopathiae-H. parasuis composition or vaccine each includes a bacterial component of inactivated E. rhusiopathiae bacteria and a suitable adjuvant. The combined E. rhusiopathiae-H. parasuis composition or vaccine further includes an amount of H. parasuis antigen. The vaccines can be administered to animals in any conventional manner. The amount of the dose for intramuscular administration is preferably less than 5 ml. The amount of E. rhusiopathiae and / or H. parasuis antigen in each dose should be enough to induce an immune response in the animal receiving the vaccine or composition and will preferably confer effective immunity against and decrease the risk of developing clinical signs resulting from E. rhusiopathiae and / or H. parasuis infection for a suitable duration of immunity.

The invention relates to application of a synthetic medium and a freeze-drying protective agent in the production of a swine erysipelas live vaccine. The synthetic medium provided by the invention is designed according to growth characteristics and growth requirements of bacillus erysipelatos-suis, is controllable in raw material sources of the formula, is not influenced by materials such as beef, beef liver, pig stomach and so on, is stable in batch, simple in manufacturing, convenient to use, suitable for carrying out high cell density culture of bacteria and high in amount of culture bacteria; in the freeze-drying process, through the adoption of the freeze-drying protective agent developed for the characteristics of the bacillus erysipelatos-suis and a matched freeze-drying curve, the activity of the bacteria is effectively protected in the freeze-drying process, so that the survive rate of the freeze-dried bacteria is increased and the vaccine is preserved for a long time at 2-8 DEG C and can be preserved for 24 months.

The invention provides an LAMP kit for detection of bacillus erysipelatos-suis.The LAMP kit comprises a primer, a Bst DNA polymerase, a reaction buffer solution and nucleic acid dye.The invention further provides an LAMP method for detection of bacillus erysipelatos-suis.Through the LAMP kit or method, bacillus erysipelatos-suis can be authenticated and detected quickly and accurately, and the LAMP kit or method has the advantages of high specificity, short consumption time, high sensitivity, easy and convenient authentication and the like and is suitable for a laboratory or field tests.

The invention provides erysipelothrix rhusiopathiae. The preservation number of the erysipelothrix rhusiopathiae is CGMCC No: 23390. The invention further provides a vaccine. The vaccine is characterized in by containing inactivated erysipelothrix rhusiopathiae with the preservation number of CGMCC No: 23390. According to the erysipelothrix rhusiopathiae, a plurality of groups of clinically separated strains are screened, and an attacking protection experiment is carried out together with a traditional swine erysipelas vaccine, so that a vaccine candidate strain which has good protection effect on epidemic type 1 and type 2 strains in swine herds in China is found.